Fig 6:
AACompSim output where the serine protease inhibitor
IPIA_TOBAC was
matched against proteins from ALL species in the
Swiss-Prot database. Many
other serine proteases of type potato chymotrypsin inhibitor I were found,
including
ICI1_LYCPE which has only 49% sequence identity with
IPIA_TOBAC.
This illustrates that proteins that belong to a family have well-conserved
amino acid compositions. Note how the proteins
POL_SIVCZ and
IPIB_TOBAC are
not present in the third list because their pI and MW do not fall within the
specified windows. The AACompSim output was shortened for this figure.
------------------------------------------
Protein IPIA_TOBAC
==================
pI: 8.80 Range: ( 8.55, 9.05)
Mw: 7891 Range: ( 6313, 9469)
The closest Swiss-Prot entries for the species ALL:
Rank Score Protein (pI Mw) Description
=================================================
1 0 IPIA_TOBAC 8.80 7891 PROTEINASE INHIBITOR I-A.
2 55 IPIB_TOBAC 9.18 7646 PROTEINASE INHIBITOR I-B.
3 59 IER1_LYCES 8.74 7914 ETHYLENE-RESPONSIVE PROTEINASE INHIB
4 60 ICID_SOLTU 8.76 8052 WOUND-INDUCED PROTEINASE INHIBITOR I.
5 61 POL_SIVCZ 9.55 20169 PROTEASE.
The closest Swiss-Prot entries:
Rank Score Protein (pI Mw) Description
=================================================
1 0 IPIA_TOBAC 8.80 7891 PROTEINASE INHIBITOR I-A.
2 55 IPIB_TOBAC 9.18 7646 PROTEINASE INHIBITOR I-B.
3 59 IER1_LYCES 8.74 7914 ETHYLENE-RESPONSIVE PROTEINASE INHIB
4 60 ICID_SOLTU 8.76 8052 WOUND-INDUCED PROTEINASE INHIBITOR I.
5 61 POL_SIVCZ 9.55 20169 PROTEASE.
The Swiss-Prot entries having pI and Mw values in the specified range:
Rank Score Protein (pI Mw) Description
=================================================
1 0 IPIA_TOBAC 8.80 7891 PROTEINASE INHIBITOR I-A.
2 59 IER1_LYCES 8.74 7914 ETHYLENE-RESPONSIVE PROTEINASE INHIB
3 60 ICID_SOLTU 8.76 8052 WOUND-INDUCED PROTEINASE INHIBITOR I.
4 97 ICI1_LYCPE 8.73 8471 WOUND-INDUCED PROTEINASE INHIBITOR I.
5 118 GRO2_RABIT 8.83 7877 GROWTH REGULATED PROTEIN HOMOLOG.
------------------------------------------