Cellosaurus HEK293 (CVCL_0045)

Cell line name HEK293
Synonyms Hek293; HEK-293; HEK 293; HEK;293; 293; 293 HEK; Human Embryonic Kidney 293
Accession CVCL_0045
Resource Identification Initiative To cite this cell line use: HEK293 (RRID:CVCL_0045)
Comments Part of: ENCODE project common cell types; tier 3.
Part of: MD Anderson Cell Lines Project.
Doubling time: ~24-30 hours (DSMZ).
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Omics: Cell surface proteome.
Omics: Deep antibody staining analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: Genome sequenced.
Omics: DNA methylation analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: Methylated arginine analysis by proteomics.
Omics: Protein expression by reverse-phase protein arrays.
Omics: Transcriptome analysis.
Omics: Virome analysis using proteomics.
Misspelling: Occasionally 'HECK293'.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_U658 (211A); CVCL_U659 (211B); CVCL_U660 (211R); CVCL_6351 (293 EcR Shh); CVCL_2285 (293 GTP-AC-free); CVCL_2286 (293 N3S); CVCL_4W25 (293 TRE/Cont); CVCL_4W26 (293 TRE/FLAG-POLK); CVCL_9832 (293-CD40); CVCL_4V25 (293-CD40L); CVCL_8468 (293-Db); CVCL_2283 (293-Hektor); CVCL_9831 (293-IL-1RI); CVCL_8469 (293-Kb); CVCL_A783 (293-LA); CVCL_2284 (293-PERV-PK-CIRCE); CVCL_M907 (293-VnR); CVCL_H362 (293/ADORA1/Galpha15); CVCL_6352 (293/CHE-Fc); CVCL_H364 (293/CRE-Luc); CVCL_J499 (293/CrmA); CVCL_H365 (293/D4); CVCL_H366 (293/EP1); CVCL_H367 (293/EP4); CVCL_H368 (293/FP/Galpha15); CVCL_H369 (293/GHRH/Galpha15); CVCL_H370 (293/H1); CVCL_Y384 (293/hMD2-CD14); CVCL_Y385 (293/hTLR1-HA); CVCL_Y386 (293/hTLR10-HA); CVCL_Y417 (293/hTLR1-TLR2); CVCL_Y387 (293/hTLR2); CVCL_Y388 (293/hTLR2-CD14); CVCL_Y389 (293/hTLR2-HA); CVCL_Y390 (293/hTLR2-TLR6); CVCL_Y391 (293/hTLR3); CVCL_Y392 (293/hTLR3-HA); CVCL_Y393 (293/hTLR4A); CVCL_Y394 (293/hTLR4-HA); CVCL_Y395 (293/hTLR4A-MD2-CD14); CVCL_Y396 (293/hTLR5); CVCL_Y397 (293/hTLR5-CD14); CVCL_Y398 (293/hTLR5-HA); CVCL_Y399 (293/hTLR6-HA); CVCL_9V14 (293/KDR); CVCL_Y101 (293/LacZ); CVCL_H372 (293/MCH1); CVCL_Y400 (293/mTLR1); CVCL_Y401 (293/mTLR1-TLR2); CVCL_Y402 (293/mTLR2); CVCL_Y403 (293/mTLR2-TLR6); CVCL_Y404 (293/mTLR3); CVCL_Y405 (293/mTLR4); CVCL_Y406 (293/mTLR4-MD2-CD14); CVCL_Y407 (293/mTLR5); CVCL_Y408 (293/mTLR6); CVCL_Y409 (293/mTLR9); CVCL_H373 (293/NPY1); CVCL_IM63 (293/pmiR-155); CVCL_IM64 (293/pmiR-K12-11); CVCL_H375 (293/TP); CVCL_E072 (293GP); CVCL_DD14 (293LAP10); CVCL_DD15 (293LAP13); CVCL_W338 (293RC21); CVCL_V648 (293sars181A); CVCL_Y381 (293XL/null); CVCL_6353 (2A); CVCL_6355 (2V6.11); CVCL_DD40 (35.32); CVCL_DD41 (37S2.8); CVCL_6361 (90.74); CVCL_6871 (AAV-293); CVCL_9804 (AD-293); CVCL_W339 (AP-1 LUCPorter); CVCL_DD22 (BHH2C); CVCL_DD23 (BHH3); CVCL_DD24 (BHH8); CVCL_9787 (C8PA); CVCL_9V15 (CRE8); CVCL_9867 (D1-HEK-293); CVCL_7261 (EcR293); CVCL_6902 (FIP293); CVCL_U421 (Flp-In-293); CVCL_U006 (Flp293A); CVCL_5285 (GFPu-1); CVCL_AQ26 (HEK 293 STF); CVCL_V349 (HEK 293 Tet-Off); CVCL_V350 (HEK 293 Tet-On 3G); CVCL_V351 (HEK 293 tTS); CVCL_GR68 (HEK-293-Munc18b); CVCL_9868 (HEK-293-TrkB); CVCL_4W07 (HEK-293.2sus); CVCL_W457 (HEK-293B1); CVCL_9869 (HEK-293B2); CVCL_9870 (HEK-293CymR); CVCL_HB82 (HEK-3KO); CVCL_IM95 (HEK-Blue hMD2-CD14); CVCL_IM80 (HEK-Blue hTLR2); CVCL_IM81 (HEK-Blue hTLR3); CVCL_IM82 (HEK-Blue hTLR4); CVCL_IM83 (HEK-Blue hTLR5); CVCL_IM84 (HEK-Blue hTLR7); CVCL_IM85 (HEK-Blue hTLR8); CVCL_IM86 (HEK-Blue hTLR9); CVCL_5I78 (HEK-Blue KD-TLR5); CVCL_IM94 (HEK-Blue mTLR13); CVCL_IM87 (HEK-Blue mTLR2); CVCL_IM88 (HEK-Blue mTLR3); CVCL_IM89 (HEK-Blue mTLR4); CVCL_IM90 (HEK-Blue mTLR5); CVCL_IM91 (HEK-Blue mTLR7); CVCL_IM92 (HEK-Blue mTLR8); CVCL_IM93 (HEK-Blue mTLR9); CVCL_E339 (HEK-CaSR); CVCL_IM79 (HEK-TLR2); CVCL_2H48 (HEK1.1); CVCL_5I96 (HEK293 (+Galpha16) AequoScreen); CVCL_0296 (HEK293 482R); CVCL_5I97 (HEK293 AequoScreen); CVCL_HC62 (HEK293 Cav3.1); CVCL_HC63 (HEK293 Cav3.2); CVCL_HC64 (HEK293 Cav3.3); CVCL_S025 (HEK293 SimpleCell O-GalNAc); CVCL_6910 (HEK293-A); CVCL_E149 (HEK293-A7); CVCL_6974 (HEK293-EBNA); CVCL_EP72 (HEK293-EPHB1); CVCL_EP73 (HEK293-EPHB2); CVCL_6996 (HEK293-ET); CVCL_6642 (HEK293-F); CVCL_6643 (HEK293-H); CVCL_5I43 (HEK293-Nav 1.3); CVCL_H513 (HEK293/5-HT2C); CVCL_H514 (HEK293/AT1); CVCL_H515 (HEK293/GCGR/Galpha15); CVCL_5A22 (HEK293/GFP-DFCP1); CVCL_H516 (HEK293/hERG); CVCL_H517 (HEK293/hKv1.5); CVCL_H518 (HEK293/hKv4.2); CVCL_H519 (HEK293/hNav1.5); CVCL_H520 (HEK293/hNav1.7); CVCL_M775 (HEK293L); CVCL_A784 (HEK293S); CVCL_0063 (HEK293T); CVCL_HB83 (HEKR1); CVCL_HB84 (HEKR2); CVCL_HB85 (HEKR2R1); CVCL_HB86 (HEKR2R3); CVCL_HB87 (HEKR3); CVCL_HB88 (HEKR3R1); CVCL_2491 (HH-8); CVCL_E065 (HKb20); CVCL_N700 (HL-2); CVCL_5I63 (K177); CVCL_0406 (MAIL8); CVCL_3772 (pEAK Rapid); CVCL_8967 (ProPak-A.52); CVCL_8968 (ProPak-A.6); CVCL_8969 (ProPak-X.36); CVCL_6448 (SODk1); CVCL_D585 (T-REx-293); CVCL_8117 (Tet-iNOS-293); CVCL_2737 (tsA201); CVCL_2767 (WSS-1)
Sex of cell Female
Category Transformed cell line
STR profile Source(s): ATCC; CLS; DSMZ; ECACC; JCRB; KCLB; PubMed=11416159; RCB; TKG

Markers:
AmelogeninX
CSF1PO11,12
D13S31712,14 (ATCC; CLS; DSMZ; ECACC; KCLB; PubMed=11416159; RCB)
12 (JCRB; TKG)
D16S5399,13 (ATCC; ECACC; JCRB; RCB; TKG)
9 (CLS; DSMZ)
D18S5118 (CLS)
17,18 (PubMed=11416159)
D21S1128,30.2
D3S135815,17
D5S8188,9
D7S82011,12
D8S117912,14
FGA23
Penta D9,10
Penta E7,15
TH017,9.3
TPOX11
vWA16,19
Web pages https://en.wikipedia.org/wiki/HEK_293_cells
http://hek293.com/
http://hek293genome.org/v2/
http://www.cells-talk.com/index.php/page/copelibrary?key=HEK293
http://www.proteinatlas.org/learn/cellines
http://141.61.102.20/mxdb/project/show/9191407937500
http://www.addexbio.com/productdetail?pid=76
http://fluoview.magnet.fsu.edu/gallery/cells/hek/hekcells.html
http://en.vircell.com/products/293-cell-line/
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/2/cell-lines-detail-176.html
http://web.archive.org/web/20140910211526/http://webserver.mbi.ufl.edu/~shaw/293.html
http://tcpaportal.org/mclp/
Publications

PubMed=886304; DOI=10.1099/0022-1317-36-1-59
Graham F.L., Smiley J., Russell W.C., Nairn R.
Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
J. Gen. Virol. 36:59-74(1977)

PubMed=21502043
Rubino M.J.
Experiences with HEK293: a human cell line.
PDA J. Pharm. Sci. Technol. 64:392-395(2010)

PubMed=21269460; DOI=10.1186/1752-0509-5-17
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.
Initial characterization of the human central proteome.
BMC Syst. Biol. 5:17-17(2011)

PubMed=22278370; DOI=10.1074/mcp.M111.014050
Geiger T., Wehner A., Schaab C., Cox J., Mann M.
Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins.
Mol. Cell. Proteomics 11:M111.014050-M111.014050(2012)

PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=24618588; DOI=10.1371/journal.pone.0091433
Chernobrovkin A.L., Zubarev R.A.
Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
PLoS ONE 9:E91433-E91433(2014)

PubMed=25182477; DOI=10.1038/ncomms5767
Lin Y.-C., Boone M., Meuris L., Lemmens I., Van Roy N., Soete A., Reumers J., Moisse M., Plaisance S., Drmanac R., Chen J., Speleman F., Lambrechts D., Van de Peer Y., Tavernier J., Callewaert N.
Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.
Nat. Commun. 5:4767-4767(2014)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

PubMed=27577262; DOI=10.1126/scisignal.aaf7329
Larsen S.C., Sylvestersen K.B., Mund A., Lyon D., Mullari M., Madsen M.V., Daniel J.A., Jensen L.J., Nielsen M.L.
Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells.
Sci. Signal. 9:Rs9-Rs9(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections ATCC; CRL-1573
ATCC; PTA-4488
BCRC; 60019
BCRJ; 0009
CCLV; CCLV-RIE 0197
CLS; 300192/p777_HEK293
DSMZ; ACC-305
ECACC; 85120602
ICLC; HTL03003
IZSLER; BS CL 129
JCRB; JCRB9068
KCB; KCB 200408YJ
KCB; KCB 200971YJ
KCLB; 21573
NCBI_Iran; C497
NIH-ARP; 103-306
RCB; RCB1637
TKG; TKG 0306
Cell line databases/resources CCRID; 3111C0001CCC000010
CCRID; 3111C0002000000005
CCRID; 3131C0001000200001
CCRID; 3131C0001000200018
CLDB; cl45
CLDB; cl46
CLDB; cl47
CLDB; cl7133
Lonza; 46
TOKU-E; 31
Ontologies BTO; BTO:0000007
CLO; CLO_0001230
CLO; CLO_0050903
EFO; EFO_0001182
MCCL; MCC:0000006
Biological sample resources BioSample; SAMN03471398
BioSample; SAMN03473347
BioSamples; SAMEA2018185
ENCODE; ENCBS022BLS
ENCODE; ENCBS209KHO
ENCODE; ENCBS264HQL
ENCODE; ENCBS375ENC
ENCODE; ENCBS393ENC
ENCODE; ENCBS421ENC
ENCODE; ENCBS439ENC
ENCODE; ENCBS479AAA
ENCODE; ENCBS480AAA
ENCODE; ENCBS481AAA
ENCODE; ENCBS482AAA
ENCODE; ENCBS483AAA
ENCODE; ENCBS484AAA
ENCODE; ENCBS486AAA
ENCODE; ENCBS522ENC
ENCODE; ENCBS523ENC
ENCODE; ENCBS613RZL
ENCODE; ENCBS906GPZ
Chemistry resources ChEMBL-Cells; CHEMBL3307715
ChEMBL-Targets; CHEMBL614818
Gene expression databases GEO; GSM1374525
GEO; GSM580027
GEO; GSM580028
GEO; GSM651571
GEO; GSM945256
GEO; GSM945288
Other Wikidata; Q489618
Polymorphism and mutation databases Cosmic; 2520629
Proteomic databases PRIDE; PXD000589
PRIDE; PXD000623
PRIDE; PXD000680
PRIDE; PXD000933
PRIDE; PXD001468
PRIDE; PXD001511
PRIDE; PXD001711
PRIDE; PXD002389
PRIDE; PXD002395
PRIDE; PXD002601
PRIDE; PXD002676
PRIDE; PXD003700
PRIDE; PXD003896