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Cellosaurus A-253 (CVCL_1060)

Cell line name A-253
Synonyms A 253; A253
Accession CVCL_1060
Resource Identification Initiative To cite this cell line use: A-253 (RRID:CVCL_1060)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Population: Caucasian.
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Array-based CGH.
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Salivary gland, submandibular gland; UBERON=UBERON_0001736.
Sequence variations
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Ala17fs*5 (c.49_61delGCCACGGCCGCGG); Zygosity=Heterozygous (ATCC; Cosmic-CLP; DepMap).
  • Mutation; HGNC; 7133; KMT2D; Simple; p.Gly3092Ter (c.9274G>T); Zygosity=Heterozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Leu152Pro (c.455T>C); ClinVar=VCV000536551; Zygosity=Homozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 11998; TP53; Simple; p.Glu180fs*67 (c.539delA); Zygosity=Homozygous (ATCC; Cosmic-CLP; DepMap).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*32:01,32:01
HLA-BB*44:02,44:02
HLA-CC*03:04,05:01
Class II
HLA-DQDQB1*06:08,06:08
HLA-DRDRB1*13:10,13:10
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.97
Native American0
East Asian, North1.81
East Asian, South0
South Asian0
European, North61.82
European, South35.39
Disease Submandibular gland squamous cell carcinoma (NCIt: C5943)
Squamous cell carcinoma of salivary glands (ORDO: Orphanet_500481)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A2EP (A-253 clone B2)CVCL_A2EQ (A-253 clone B4)CVCL_A2ER (A-253 clone B9)
CVCL_A2ES (A-253 clone D3)CVCL_A2ET (A-253 clone F11)
Sex of cell Male
Age at sampling 54Y
Category Cancer cell line
STR profile Source(s): ATCC; Cosmic-CLP; IZSLER; PubMed=29923277

Markers:
AmelogeninX
CSF1PO12,13
D5S81811
D7S82010,12
D13S3179,12
D16S53912
D21S1130,30.2
TH016,8
TPOX8,12
vWA16

Run an STR similarity search on this cell line
Publications

PubMed=4357758; DOI=10.1093/jnci/51.5.1417
Giard D.J., Aaronson S.A., Todaro G.J., Arnstein P., Kersey J.H., Dosik H., Parks W.P.
In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
J. Natl. Cancer Inst. 51:1417-1423(1973)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=6256643; DOI=10.1038/288724a0
Day R.S. III, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Lubiniecki A.S., Girardi A.J., Galloway S.M., Bynum G.D.
Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains.
Nature 288:724-727(1980)

PubMed=22282976; DOI=10.1093/carcin/1.1.21
Day R.S. III, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Mattern M.R.
Human tumor cell strains defective in the repair of alkylation damage.
Carcinogenesis 1:21-32(1980)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3138611; DOI=10.1177/019459988809800603
Wustrow T.P.U., Raffael A., Valet G.K.
Multiparametric flow cytometry of human squamous cell carcinoma lines from the head and neck.
Otolaryngol. Head Neck Surg. 98:552-557(1988)

DOI=10.1016/B978-0-12-333530-2.50007-1
Carey T.E.
Head and neck tumor cell lines.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.79-120; Academic Press; New York (1994)

PubMed=20014447; DOI=10.1002/hed.21311
Tsui I.F.L., Garnis C.
Integrative molecular characterization of head and neck cancer cell model genomes.
Head Neck 32:1143-1160(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29923277; DOI=10.1111/odi.12920
Lin L.-C., Elkashty O., Ramamoorthi M., Trinh N., Liu Y.-N., Sunavala-Dossabhoy G., Pranzatelli T., Michael D.G., Chivasso C., Perret J., Chiorini J.A., Delporte C., Tran S.D.
Cross-contamination of the human salivary gland HSG cell line with HeLa cells: a STR analysis study.
Oral Dis. 24:1477-1483(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-7902 - Discontinued
ATCC; HTB-41
BCRC; 60367
BCRJ; 0322
IZSLER; BS TCL 199
KCLB; 30041 - Discontinued
Cell line databases/resources CLO; CLO_0001569
CLO; CLO_0001570
cancercelllines; CVCL_1060
Cell_Model_Passport; SIDM00796
Cosmic-CLP; 906794
DepMap; ACH-000740
LINCS_LDP; LCL-2034
TOKU-E; 462
Anatomy/cell type resources BTO; BTO:0003206
Biological sample resources BioSample; SAMN03471117
BioSample; SAMN10988034
CRISP screens repositories BioGRID_ORCS_Cell_line; 824
Chemistry resources ChEMBL-Cells; CHEMBL3307893
ChEMBL-Targets; CHEMBL614255
GDSC; 906794
PharmacoDB; A253_39_2019
PubChem_Cell_line; CVCL_1060
Encyclopedic resources Wikidata; Q54605985
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM422867
GEO; GSM423670
GEO; GSM886852
GEO; GSM887917
GEO; GSM1669580
Polymorphism and mutation databases Cosmic; 688036
Cosmic; 906794
Cosmic; 921831
Cosmic; 1017790
Cosmic; 1006471
Cosmic; 1041671
Cosmic; 1995330
Cosmic; 2646649
IARC_TP53; 21160
LiGeA; CCLE_437
Progenetix; CVCL_1060
Proteomic databases PRIDE; PXD002486
PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number40