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Cellosaurus NCI-H660 (CVCL_1576)

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Cell line name NCI-H660
Synonyms H660; H-660; NCIH660; NCI660
Accession CVCL_1576
Resource Identification Initiative To cite this cell line use: NCI-H660 (RRID:CVCL_1576)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 100 hours (PubMed=25984343).
Microsatellite instability: Stable (MSS) (PubMed=23671654; Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Caution: Was originally published as originating from a small cell lung carcinoma (PubMed=2985257), but was later shown to originate from a prostate small cell carcinoma (PubMed=2569043).
Misspelling: NCI_H600; Cosmic=2669168; Note=Prior to 2020.
Derived from site: Metastatic; Cervical lymph node; UBERON=UBERON_0002429.
Sequence variations
  • Gene fusion; HGNC; 3446; ERG + HGNC; 11876; TMPRSS2; Name(s)=TMPRSS2-ERG (PubMed=17401460).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,68:01
HLA-BB*44:02,57:01
HLA-CC*07:04,07:04
Genome ancestry Source: PubMed=30894373

Origin% genome
African2.18
Native American0
East Asian, North2.77
East Asian, South0
South Asian0
European, North58.05
European, South37
Disease Prostate small cell carcinoma (NCIt: C6766)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 63Y
Category Cancer cell line
STR profile Source(s): ATCC; Cosmic-CLP; PubMed=11304728; PubMed=14518029; PubMed=25877200

Markers:
AmelogeninX
CSF1PO12
D3S135814,18
D5S81811
D7S8208,10
D8S11799,10
D13S3178
D16S53911
D18S5115,16
D21S1128,31
FGA23,24
Penta D9,12
Penta E12
TH017,9.3
TPOX8
vWA17

Run an STR similarity search on this cell line
Publications

PubMed=2985257
Carney D.N., Gazdar A.F., Bepler G., Guccion J.G., Marangos P.J., Moody T.W., Zweig M.H., Minna J.D.
Establishment and identification of small cell lung cancer cell lines having classic and variant features.
Cancer Res. 45:2913-2923(1985)

PubMed=2569043; DOI=10.1093/jnci/81.16.1223
Johnson B.E., Whang-Peng J., Naylor S.L., Zbar B., Brauch H., Lee E., Simmons A.M., Russell E., Nam M.H., Gazdar A.F.
Retention of chromosome 3 in extrapulmonary small cell cancer shown by molecular and cytogenetic studies.
J. Natl. Cancer Inst. 81:1223-1228(1989)

PubMed=1563005
Giaccone G., Battey J., Gazdar A.F., Oie H.K., Draoui M., Moody T.W.
Neuromedin B is present in lung cancer cell lines.
Cancer Res. 52:2732s-2736s(1992)

PubMed=8806092; DOI=10.1002/jcb.240630505
Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. 63 Suppl. 24:32-91(1996)

PubMed=8806103; DOI=10.1002/jcb.240630516
Johnson B.E., Russell E., Simmons A.M., Phelps R.M., Steinberg S.M., Ihde D.C., Gazdar A.F.
MYC family DNA amplification in 126 tumor cell lines from patients with small cell lung cancer.
J. Cell. Biochem. 63 Suppl. 24:210-217(1996)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=11304728; DOI=10.1002/pros.1045
van Bokhoven A., Varella-Garcia M., Korch C.T., Hessels D., Miller G.J.
Widely used prostate carcinoma cell lines share common origins.
Prostate 47:36-51(2001)

PubMed=14518029; DOI=10.1002/pros.10290
van Bokhoven A., Varella-Garcia M., Korch C.T., Johannes W.U., Smith E.E., Miller H.L., Nordeen S.K., Miller G.J., Lucia M.S.
Molecular characterization of human prostate carcinoma cell lines.
Prostate 57:205-225(2003)

CLPUB00698
van Bokhoven A.
Models for prostate cancer. Molecular characterization and critical appraisal of human prostate carcinoma cell lines.
Thesis PhD (2004), Katholieke Universiteit Nijmegen, Netherlands

PubMed=15643172; DOI=10.1097/01.ju.0000141580.30910.57
Sobel R.E., Sadar M.D.
Cell lines used in prostate cancer research: a compendium of old and new lines -- part 1.
J. Urol. 173:342-359(2005)

PubMed=17401460; DOI=10.1593/neo.07103
Mertz K.D., Setlur S.R., Dhanasekaran S.M., Demichelis F., Perner S., Tomlins S., Tchinda J., Laxman B., Vessella R.L., Beroukhim R., Lee C., Chinnaiyan A.M., Rubin M.A.
Molecular characterization of TMPRSS2-ERG gene fusion in the NCI-H660 prostate cancer cell line: a new perspective for an old model.
Neoplasia 9:200-206(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23671654; DOI=10.1371/journal.pone.0063056
Lu Y.-H., Soong T.D., Elemento O.
A novel approach for characterizing microsatellite instability in cancer cells.
PLoS ONE 8:E63056-E63056(2013)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-5813
Cell line databases/resources CLO; CLO_0008102
cancercelllines; CVCL_1576
Cell_Model_Passport; SIDM01123
Cosmic-CLP; 1330975
DepMap; ACH-000177
IGRhCellID; NCIH660
Anatomy/cell type resources BTO; BTO:0001970
Biological sample resources BioSample; SAMN03473052
BioSample; SAMN10988121
Chemistry resources PharmacoDB; NCIH660_1126_2019
Encyclopedic resources Wikidata; Q54908097
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM482672
GEO; GSM648822
GEO; GSM887440
GEO; GSM888520
GEO; GSM1670256
Polymorphism and mutation databases Cosmic; 877360
Cosmic; 1021869
Cosmic; 1043331
Cosmic; 1075272
Cosmic; 1689713
Cosmic; 2651769
Cosmic; 2669168
IARC_TP53; 21084
IARC_TP53; 21725
LiGeA; CCLE_659
Progenetix; CVCL_1576
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number34