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Cellosaurus RTG-2 (CVCL_1920)

[Text version]
Cell line name RTG-2
Synonyms RTG 2; RTG2; Rainbow Trout Gonad-2
Accession CVCL_1920
Resource Identification Initiative To cite this cell line use: RTG-2 (RRID:CVCL_1920)
Comments Group: Fish cell line.
Doubling time: ~2 days (Note=At 20 Celsius) (PubMed=5167740; PubMed=3410806); ~3-7 days (DSMZ=ACC-352).
Anecdotal: First fish cell line to have been established, in 1962.
Misspelling: RTG-22; Note=Occasionally.
Misspelling: RTG22; Note=Occasionally.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Fibroblast of ovary; CL=CL_2000063.
Species of origin Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) (NCBI Taxonomy: 8022)
Hierarchy Children:
CVCL_0I50 (RTG-2/f)CVCL_1921 (RTG-P1)
Sex of cell Female
Age at sampling Yearling
Category Spontaneously immortalized cell line
Publications

PubMed=14007940; DOI=10.1126/science.135.3508.1065
Wolf K., Quimby M.C.
Established eurythermic line of fish cells in vitro.
Science 135:1065-1066(1962)

DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=5167740; DOI=10.1007/BF02619003
Plumb J.A., Wolf K.
Fish cell growth rates. Quantitative comparison of RTG-2 cell growth at 5-25 degrees C.
In Vitro 7:42-45(1971)

PubMed=235493; DOI=10.1128/IAI.11.4.815-822.1975
De Sena J., Rio G.J.
Partial purification and characterization of RTG-2 fish cell interferon.
Infect. Immun. 11:815-822(1975)

DOI=10.1139/f78-164
Kelly R.K., Souter B.W., Miller H.R.
Fish cell lines: comparisons of CHSE-214, FHM, and RTG-2 in assaying IHN and IPN viruses.
J. Fish. Res. Board Can. 35:1009-1011(1978)

DOI=10.3354/dao001209
Kimura T., Yoshimizu M., Gorie S.
A new rhabdovirus isolated in Japan from cultured hirame (Japanese flounder) Paralichthys olivaceus and ayu Plecoglossus altivelis.
Dis. Aquat. Organ. 1:209-217(1986)

CLPUB00103
Yoshimizu M., Kamei M., Dirakbusarakom S., Kimura T.
Fish cell lines: susceptibility to salmonid viruses.
(In) Invertebrate and fish tissue culture; Kuroda Y., Kurstak E., Maramorosch K. (eds.); pp.207-217; Japan Sci. Soc.; Tokyo (1988)

PubMed=3410806; DOI=10.1007/BF02623650
Lee L.E.J., Martinez A., Bols N.C.
Culture conditions for arresting and stimulating the proliferation of a rainbow trout fibroblast cell line, RTG-2.
In Vitro Cell. Dev. Biol. 24:795-802(1988)

DOI=10.1111/j.1365-2761.1989.tb01290.x
Frerichs G.N., Hill B.J., Way K.
Ulcerative disease rhabdovirus: cell-line susceptibility and serological comparison with other fish rhabdoviruses.
J. Fish Dis. 12:51-56(1989)

PubMed=2591197; DOI=10.1016/0305-0491(89)90357-x
Tocher D.R., Carr J., Sargent J.R.
Polyunsaturated fatty acid metabolism in fish cells: differential metabolism of (n-3) and (n-6) series acids by cultured cells originating from a freshwater teleost fish and from a marine teleost fish.
Comp. Biochem. Physiol. 94B:367-374(1989)

PubMed=1368400; DOI=10.1007/BF02540026
Ganassin R.C., Bols N.C.
Effect of purine supplementation on the growth of salmonid cell lines in different mammalian sera.
Cytotechnology 8:21-29(1992)

DOI=10.3147/jsfp.28.27
Fernandez R.D., Yoshimizu M., Ezura Y., Kimura T.
Comparative growth response of fish cell lines in different media, temperatures, and and sodium chloride concentrations.
Fish Pathol. 28:27-34(1993)

DOI=10.3147/jsfp.30.23
Oh M.-J., Yoshimizu M., Kimura T., Ezura Y.
A new virus isolated from salmonid fish.
Fish Pathol. 30:23-32(1995)

PubMed=8736390; DOI=10.1177/019262339602400310
Carter C.A., Ellington W.W., Van Beneden R.J.
Confocal laser scanning microscopy of oncogene localization in rainbow trout cell lines derived from normal and tumor tissue.
Toxicol. Pathol. 24:339-345(1996)

PubMed=11501281; DOI=10.1002/tox.1039
Nehls S., Segner H.
Detection of DNA damage in two cell lines from rainbow trout, RTG-2 and RTL-W1, using the comet assay.
Environ. Toxicol. 16:321-329(2001)

PubMed=19002917; DOI=10.1023/A:1020516804173
Perry G.M.L., McDonald G.J., Ferguson M.M., Ganassin R.C., Bols N.C.
Characterization of rainbow trout cell lines using microsatellite DNA profiling.
Cytotechnology 37:143-151(2001)

DOI=10.1016/S1873-0140(05)80005-0
Bols N.C., Dayeh V.R., Lee L.E.J., Schirmer K.
Use of fish cell lines in the toxicology and ecotoxicology of fish Piscine cell lines in environmental toxicology.
(In) Biochemistry and molecular biology of fishes, Vol. 6; Mommsen T.P., Moon T.W. (eds.); pp.43-84; Elsevier; Amsterdam (2005)

PubMed=20420918; DOI=10.1016/j.fsi.2010.04.007
Kawano A., Kales S.C., Fujiki K., DeWitte-Orr S.J., Dixon B., Lee L.E.J., Bols N.C.
A comparison of rainbow trout cell lines for their expression of the major histocompatibility complex genes and the induction of beta-2-microglobulin by dsRNA.
Fish Shellfish Immunol. 29:312-318(2010)

PubMed=28374170; DOI=10.1007/s11626-017-0142-5
Bols N.C., Pham P.H., Dayeh V.R., Lee L.E.J.
Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout.
In Vitro Cell. Dev. Biol. Anim. 53:383-405(2017)

PubMed=33053677; DOI=10.3390/pathogens9100833
Pham P.H., Misk E., Papazotos F., Jones G., Polinski M.P., Contador E., Russell S., Garver K.A., Lumsden J.S., Bols N.C.
Screening of fish cell lines for piscine orthoreovirus-1 (PRV-1) amplification: identification of the non-supportive PRV-1 invitrome.
Pathogens 9:833.1-833.25(2020)

PubMed=34382581; DOI=10.3354/dao03615
Ito T., Kamaishi T.
Japanese amberjack Seriola quinqueradiata and red sea bream Pagrus major susceptibility to infectious hematopoietic necrosis virus (IHNV) isolate.
Dis. Aquat. Organ. 146:1-8(2021)

Cross-references
Cell line collections (Providers) ATCC; CCL-55
BCRC; 60445
CCLV; CCLV-RIE 0038
CCLV; CCLV-RIE 0088
CCTCC; GDC0169
DSMZ; ACC-352
ECACC; 90102529
IZSLER; BS CL 76
KCB; KCB 89021YJ
Cell line databases/resources CLO; CLO_0008895
CLDB; cl4202
CLDB; cl4203
CLDB; cl4204
DSMZCellDive; ACC-352
Anatomy/cell type resources BTO; BTO:0005472
Encyclopedic resources Wikidata; Q54951397
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number28