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Cellosaurus COLO 818 (CVCL_1998)

Cell line name COLO 818
Synonyms COLO-818; COLO #818; COLO818; Colorado 818
Accession CVCL_1998
Resource Identification Initiative To cite this cell line use: COLO 818 (RRID:CVCL_1998)
Comments Problematic cell line: Contaminated. Shown to be a COLO 800 derivative (PubMed=10508494; PubMed=20143388).
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00114.
Population: Caucasian.
Doubling time: ~60 hours (DSMZ=ACC-81).
Omics: Deep exome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: Metastatic; Hypodermis; UBERON=UBERON_0002072.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (DepMap).
  • Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Cys135Arg (c.403T>C); ClinVar=VCV000376560; Zygosity=Heterozygous (DepMap).
HLA typing Source: PubMed=15592718
Class I
HLA-AA*02:01,29:02
HLA-BB*44:02,44:03:01
HLA-CC*05:01,16:01
Class II
HLA-DPDPB1*04:01,17:01
HLA-DQDQB1*03:01:01,02:01
HLA-DRDRB1*12:01,07:01

Source: PubMed=26589293
Class I
HLA-AA*02:01,29:02
HLA-BB*44:03,44:03
HLA-CC*05:01,16:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North0
East Asian, South1.07
South Asian3.33
European, North39.88
European, South55.71
Disease Cutaneous melanoma (NCIt: C3510)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1135 (COLO 800)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): DSMZ; ESTDAB; PubMed=25877200

Markers:
AmelogeninX,Y
CSF1PO10
D2S133819
D3S135818
D5S81811
D7S82010,11
D8S117914,15
D13S31711,13
D16S53911
D18S5113,14
D19S43314
D21S1129,30
FGA23
Penta D11,13
Penta E7
TH016
TPOX11
vWA16,20

Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v12_distribution.xlsx
Publications

PubMed=7780963
Easty D.J., Guthrie B.A., Maung K., Farr C.J., Lindberg R.A., Toso R.J., Herlyn M., Bennett D.C.
Protein B61 as a new growth factor: expression of B61 and up-regulation of its receptor epithelial cell kinase during melanoma progression.
Cancer Res. 55:2528-2532(1995)

PubMed=10508494; DOI=10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2
MacLeod R.A.F., Dirks W.G., Matsuo Y., Kaufmann M., Milch H., Drexler H.G.
Widespread intraspecies cross-contamination of human tumor cell lines arising at source.
Int. J. Cancer 83:555-563(1999)

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=15592718; DOI=10.1007/s00262-004-0561-5
Rodriguez T., Mendez R., Roberts C.H., Ruiz-Cabello F., Dodi I.A., Lopez Nevot M.A., Paco L., Maleno I., Marsh S.G.E., Pawelec G., Garrido F.
High frequency of homozygosity of the HLA region in melanoma cell lines reveals a pattern compatible with extensive loss of heterozygosity.
Cancer Immunol. Immunother. 54:141-148(2005)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections (Providers) DSMZ; ACC-81
ECACC; 93052622 - Discontinued
Cell line databases/resources CLO; CLO_0002562
CLO; CLO_0037122
CLDB; cl855
CLDB; cl169
cancercelllines; CVCL_1998
Cell_Model_Passport; SIDM01153
DepMap; ACH-001044
DSMZCellDive; ACC-81
ESTDAB; ESTDAB-003
LINCS_LDP; LCL-1244
Biological sample resources BioSample; SAMN03151628
BioSample; SAMN03473279
BioSample; SAMN10988106
Chemistry resources PharmacoDB; COLO818_236_2019
Encyclopedic resources Wikidata; Q54814155
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM886952
GEO; GSM888020
Polymorphism and mutation databases Cosmic; 972257
IARC_TP53; 28387
Progenetix; CVCL_1998
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number36