Cellosaurus cell line ECV-304 (CVCL_2029)

• Mutation; HGNC; 5173; HRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012600; Zygosity=Homozygous (from parent cell line).
  
• Mutation; HGNC; 11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  
• Mutation; HGNC; 11998; TP53; Simple; p.Tyr126Ter (c.378C>G); ClinVar=VCV000578988; Zygosity=Homozygous (from parent cell line).
  

PubMed=1690702; DOI=10.1007/BF02624456
Takahashi K., Sawasaki Y., Hata J.-i., Mukai K., Goto T.
Spontaneous transformation and immortalization of human endothelial cells.
In Vitro Cell. Dev. Biol. 26:265-274(1990)

PubMed=1817644
Kobayashi M., Kondo M., Mitsui Y.
Establishment of human endothelial cell lines in a serum-free culture and its application for expression of transfected prepro endothelin gene.
Hum. Cell 4:296-305(1991)

PubMed=1378828; DOI=10.1007/BF02634037
Takahasi K., Sawasaki Y.
Rare spontaneously transformed human endothelial cell line provides useful research tool.
In Vitro Cell. Dev. Biol. Anim. 28:380-382(1992)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

DOI=10.11418/jtca1981.18.4_329
Tanabe H., Takada Y., Minegishi D., Kurematsu M., Masui T., Mizusawa H.
Cell line individualization by STR multiplex system in the cell bank found cross-contamination between ECV304 and EJ-1/T24.
Tissue Cult. Res. Commun. 18:329-338(1999)

PubMed=10508494; DOI=10.1002/(SICI)1097-0215(19991112)83:4<555::AID-IJC19>3.0.CO;2-2
MacLeod R.A.F., Dirks W.G., Matsuo Y., Kaufmann M., Milch H., Drexler H.G.
Widespread intraspecies cross-contamination of human tumor cell lines arising at source.
Int. J. Cancer 83:555-563(1999)

PubMed=10614862; DOI=10.1007/s11626-999-0091-8
Dirks W.G., MacLeod R.A.F., Drexler H.G.
ECV304 (endothelial) is really T24 (bladder carcinoma): cell line cross-contamination at source.
In Vitro Cell. Dev. Biol. Anim. 35:558-559(1999)

PubMed=10653001; DOI=10.1038/labinvest.3780006
Brown J., Reading S.J., Jones S., Fitchett C.J., Howl J., Martin A., Longland C.L., Michelangeli F., Dubrova Y.E., Brown C.A.
Critical evaluation of ECV304 as a human endothelial cell model defined by genetic analysis and functional responses: a comparison with the human bladder cancer derived epithelial cell line T24/83.
Lab. Invest. 80:37-45(2000)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11669284; DOI=10.1290/1071-2690(2001)037<0505:PCOHUV>2.0.CO;2
Suda K., Rothen-Rutishauser B., Gunthert M., Wunderli-Allenspach H.
Phenotypic characterization of human umbilical vein endothelial (ECV304) and urinary carcinoma (T24) cells: endothelial versus epithelial features.
In Vitro Cell. Dev. Biol. Anim. 37:505-514(2001)

PubMed=12197766; DOI=10.1290/1071-2690(2002)038<0185:BCCLEI>2.0.CO;2
Drexler H.G., Quentmeier H., Dirks W.G., MacLeod R.A.F.
Bladder carcinoma cell line ECV304 is not a model system for endothelial cells.
In Vitro Cell. Dev. Biol. Anim. 38:185-186(2002)

PubMed=12453433; DOI=10.1006/mvre.2002.2434
Unger R.E., Krump-Konvalinkova V., Peters K., Kirkpatrick C.J.
In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.
Microvasc. Res. 64:384-397(2002)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=27554326; DOI=10.1038/srep31811
Xie Y.-J., Wang J.-L., Zhang Y.-Y., Liu X.-F., Wang X.-R., Liu K.-H., Huang X.-H., Wang Y.-C.
Quantitative profiling of spreading-coupled protein tyrosine phosphorylation in migratory cells.
Sci. Rep. 6:31811-31811(2016)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)