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Cellosaurus OSC-20 (CVCL_3087)

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Cell line name OSC-20
Synonyms OSC20
Accession CVCL_3087
Resource Identification Initiative To cite this cell line use: OSC-20 (RRID:CVCL_3087)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Doubling time: 27.4 hours (Note=At 35th passage) (DOI=10.15114/tr.25.93); 65 hours (Note=Lot 02032003), ~49 hours (Note=Lot 11152011), ~35 hours (Note=Lot 07182019) (JCRB=JCRB0197).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Derived from site: Metastatic; Cervical lymph node; UBERON=UBERON_0002429.
Sequence variations
  • Mutation; HGNC; 1787; CDKN2A; Simple; p.Pro81Leu (c.242C>T); ClinVar=VCV000833629; Zygosity=Homozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 8975; PIK3CA; Simple; p.His1048Arg (c.3143A>G); ClinVar=VCV000450316; Zygosity=Heterozygous (Cosmic-CLP; DepMap).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg249Ser (c.747G>T); ClinVar=VCV000012352; Zygosity=Homozygous (Cosmic-CLP; DepMap).
HLA typing Source: PubMed=9119748
Class I
HLA-AA*02,11
HLA-BB*46,55
HLA-CC*w01,w09
Genome ancestry Source: PubMed=30894373

Origin% genome
African3.14
Native American0.23
East Asian, North75.72
East Asian, South16.66
South Asian0
European, North0
European, South4.24
Disease Tongue squamous cell carcinoma (NCIt: C4648)
Squamous cell carcinoma of the oral tongue (ORDO: Orphanet_457252)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individual CVCL_WW78 ! TcOSC-20
Sex of cell Female
Age at sampling 59Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP; DepMap

Markers:
AmelogeninX
CSF1PO12,13
D3S135816
D5S81813 (Cosmic-CLP)
13,14 (DepMap)
D7S82010
D8S117912,14
D13S3178
D16S5399,13
D18S5113
D21S1129,32.2
FGA22
Penta D9,12
Penta E14,16
TH019,9.3
TPOX8,11
vWA18

Run an STR similarity search on this cell line
Publications

DOI=10.15114/tr.25.93
Yokoi T., Hirata S., Nishimura F., Miyakawa A., Odajima T., Kohama G.-i., Mochizuki Y.
Some properties of a newly established human cell line from a oral squamous carcinoma.
Tumor Res. 25:93-103(1990)

PubMed=9119748; DOI=10.1111/j.1349-7006.1997.tb00365.x
Miyazaki A., Sato N., Takahashi S., Sasaki A., Kohama G.-i., Yamaguchi A., Yagihashi A., Kikuchi K.
Cytotoxicity of histocompatibility leukocyte antigen-DR8-restricted CD4 killer T cells against human autologous squamous cell carcinoma.
Jpn. J. Cancer Res. 88:191-197(1997)

DOI=10.15114/tr.35.25
Yonekura N., Yokosawa N., Hariya Y., Dehari H., Kohama G.-i., Fujii N.
Interferon-alpha and interferon-gamma augment apoptosis in oral carcinoma cells treated with Shiga toxin 2 (Stx2).
Tumor Res. 35:25-33(2000)

PubMed=12716470; DOI=10.1111/j.1349-7006.2002.tb01338.x
Kondo H., Sahara H., Miyazaki A., Nabeta Y., Hirohashi Y., Kanaseki T., Yamaguchi A., Yamada N., Hirayama K., Suzuki M., Hamuro J., Torigoe T., Takahashi N., Kohama G.-i., Ikeda H., Sato N.
Natural antigenic peptides from squamous cell carcinoma recognized by autologous HLA-DR8-restricted CD4+ T cells.
Jpn. J. Cancer Res. 93:917-924(2002)

PubMed=19123955; DOI=10.1186/1479-5876-7-1
Kobayashi J.-i., Torigoe T., Hirohashi Y., Idenoue S., Miyazaki A., Yamaguchi A., Hiratsuka H., Sato N.
Comparative study on the immunogenicity between an HLA-A24-restricted cytotoxic T-cell epitope derived from survivin and that from its splice variant survivin-2B in oral cancer patients.
J. Transl. Med. 7:1.1-1.11(2009)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; JCRB0197
JCRB; FDSC0051 - Discontinued
Cell line databases/resources CLO; CLO_0009950
cancercelllines; CVCL_3087
Cell_Model_Passport; SIDM00565
Cosmic-CLP; 1240196
DepMap; ACH-001626
LINCS_LDP; LCL-1220
CRISP screens repositories BioGRID_ORCS_Cell_line; 979
Chemistry resources GDSC; 1240196
PharmacoDB; OSC20_1206_2019
Encyclopedic resources Wikidata; Q54936583
Gene expression databases ArrayExpress; E-MTAB-3610
GEO; GSM827220
GEO; GSM1670309
Polymorphism and mutation databases Cosmic; 1120702
Cosmic; 1995604
Progenetix; CVCL_3087
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number33