Cellosaurus logo
expasy logo

Cellosaurus J.CaM2 (CVCL_R862)

[Text version]
Cell line name J.CaM2
Synonyms JCaM2; JCam2; Jurkat-derived Ca2+ mutant no. 2
Accession CVCL_R862
Resource Identification Initiative To cite this cell line use: J.CaM2 (RRID:CVCL_R862)
Comments Population: Caucasian.
Characteristics: Lacks LAT expression.
Transformant: NCIt; C129643; Cesium-137 gamma radiation.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: T-cell; CL=CL_0000084.
Sequence variations
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Argfs*19 (c.121delG); ClinVar=VCV000009512; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 959; BAX; Simple; p.Glu41Glyfs*33 (c.121dupG); ClinVar=VCV000009511; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg505Cys (c.1513C>T); ClinVar=VCV000069961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; p.Gln345Ter (c.1033C>T); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6079; INPP5D; Simple; c.1097+1065_1097+1112del47; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 18874; LAT; Simple; g.237C>T; Zygosity=Heterozygous (PubMed=27278128; PubMed=33562083).
  • Mutation; HGNC; 18874; LAT; Simple; p.Thr56Met (c.167C>T); ClinVar=VCV001493770; Zygosity=Heterozygous (PubMed=27278128; PubMed=33562083).
  • Mutation; HGNC; 7325; MSH2; Simple; p.Arg711Ter (c.2131C>T); ClinVar=VCV000090903; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 11491; SYK; Simple; p.Met34Hisfs*3 (c.98_99insG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg196Ter (c.586C>T); ClinVar=VCV000043589; Zygosity=Heterozygous (from parent cell line).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0367 (Jurkat E6.1)
Children:
CVCL_DR61 (J.CaM2.5)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
Publications

PubMed=3263650; DOI=10.1073/pnas.85.22.8613
Goldsmith M.A., Dazin P.F., Weiss A.
At least two non-antigen-binding molecules are required for signal transduction by the T-cell antigen receptor.
Proc. Natl. Acad. Sci. U.S.A. 85:8613-8617(1988)

PubMed=9846483; DOI=10.1016/S1074-7613(00)80659-7
Finco T.S., Kadlecek T., Zhang W.-G., Samelson L.E., Weiss A.
LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway.
Immunity 9:617-626(1998)

PubMed=27278128; DOI=10.1038/gene.2016.25
Marek-Bukowiec K., Aguado E., Miazek A.
Phorbol ester-mediated re-expression of endogenous LAT adapter in J.CaM2 cells: a model for dissecting drivers and blockers of LAT transcription.
Genes Immun. 17:313-320(2016)

PubMed=33562083; DOI=10.3390/cells10020343
Vico-Barranco I., de Arbulo-Echevarria M.M., Serrano-Garcia I., Perez-Linaza A., Miranda-Sayago J.M., Miazek A., Narbona-Sanchez I., Aguado E.
A novel, LAT/Lck double deficient T cell subline J.CaM1.7 for combined analysis of early TCR signaling.
Cells 10:343.1-343.20(2021)

Cross-references
Cell line databases/resources cancercelllines; CVCL_R862
Encyclopedic resources Wikidata; Q54898428
Entry history
Entry creation05-Nov-2013
Last entry update30-Jan-2024
Version number20