pIcarver is a tool to visualize theoretical distributions of peptide pI on a given pH range, and proposes a fractionation scheme that generates fractions with similar peptide frequencies.
This tool has been developed as a service of the Bioinformatics Core Facility for Proteomics, within a collaboration between the Proteome Informatics Group from the Swiss Institute of Bioinformatics (SIB) and the Biomedical Proteomics Research Group (BPRG).
Java Web Start technology can be used to run
pIcarver directly from the ExPASy website.
Be sure to have at least version 1.5 of Java Runtime Environment (JRE) installed on your computer. Otherwise, you can download the latest version from the Sun website.
It has been fully tested on Windows 2000/XP (Firefox 2.0, IE 6.0), Linux (Firefox 2.0), Mac OS X (Safari 3.0). Do not hesitate to contact us with your configuration (OS & browser name and version) if you encountered some troubles.
NB: as first parameter of its user interface, pIcarver requires an input list of the peptides expected in the studied sample, as well as their corresponding pI. This information must be provided as a file where each line must contain three elements, the AC of a protein (or any text, excluding whitespaces), the peptide sequence and finally its pI. You may use our Compute pI/Mw tool to compute these values.
This example file contains all Staphylococcus aureus (N315) peptides and can be used to test the program.
New features in v3.1 (7/12/07)
The pIcarver now takes the position (given in %) of pI markers with Known pI values into account in order to adjust the theoretical pH gradient provided by the supplier to the real pH gradient used for the experiment.
Features in v3.0 (16/05/07)
The pIcarver contains now 3 cutting modes, "exact", "multiple" and the old "minimum" mode. Moreover, it provides a window to freely compute either pI or Volume(%) for any available pH gradient.
pIcarver v2.0 (06/03/2007) : First version available.