Issue 63 October 2005 ( PDF )

The colour of the rose

by Vivienne Baillie Gerritsen

One of the beauties of Autumn is the firework of orange, yellow and red hues it displays. Anthocyanin is a plant pigment involved in this colourful palette. And not only in the autumnal shades but also in the reds, blues and purples of petals and fruit all year round. The array of colours offered to us by Nature has always fascinated scientists who have put a great deal of effort into understanding both the structure of the various pigments but also the pathways leading to their synthesis. The final steps leading to anthocyanin are performed by an enzyme known as anthocyanidin glucosyltransferase. And in roses, this particular enzyme catalyses not one reaction – as is the case in the production of other flower anthocyanins known to date – but two reactions which lead to rose anthocyanin.

« Anthocyanin is a plant pigment which is not only at the heart of the autumnal colors but also the reds, blues and purples of petals and fruit all year round.»

Roses have been exhibiting their colours in gardens for thousands of years – the first rose gardens seem to have been Sumerian – and incessant crossbreeding has produced the many tints we know today. In 1840, the horticultural societies of Britain and Belgium offered a 500’000 franc prize to those who could produce a blue flower. Why blue? Simply because blue roses do not exist, yet they seem to have all the necessary ingredients to conjure up such a colour. It was perhaps an event like this that sparked the scientific desire to know what pigments are on a more intimate level.

The German chemist Richard Willstätter (1872-1942) made the first breakthrough. Not towards the growth of blue roses but towards a greater understanding of the chemical structure of plant pigments and how colours could vary from plant to plant. And, as a result, he was rewarded the 1915 Nobel Prize in Chemistry. He demonstrated that anthocyanins are glucosides that can be split into glucose – mainly – and a colour component, cyanidin. He also showed that the colour of a plant is not only dependent on the pigment’s structure but also the plant’s sap – or to be more precise its physiological pH – and the concentration of the pigments themselves.

[ A rose bud in dusk. Courtesy of Bruce M. Burton. ]

A rose bud in dusk

Courtesy of Bruce M. Burton

Surprisingly, despite Willstätter’s clear sightedness, he – amongst others – did not believe that enzymes were biological entities but ‘merely’ chemical substances. And it was only in the 1930s that the notion of an enzyme as something truly biological was agreed upon universally. Willstätter’s work was interrupted by World War I when he was asked to turn his attention to the making of a gas mask… After the war, he pursued his research, which was interrupted a second time by World War II. Of Jewish background, Willstätter decided to leave Germany in 1939 for Switzerland where he died only three years later. In the decades that followed, the different enzymes and chemical entities which are part of anthocyanin biosynthesis were revealed, and today the pathway is known in its molecular detail and entirety.

« Roses have a very particular anthocyanin biosynthesis pathway. Though why remains a mystery. »

Anthocyanin glucosyltransferase is present in all plants. Roses, however, have their specific transferase: an anthocyanidin 5,3-O-glucosyltransferase, which catalyses two succeeding glycosylation reactions, as opposed to only one. The enzyme’s first substrate is anthocyanidin. Anthocyanidins are low molecular weight phenylbenzopyrones – or flavonoids – of which there are over 4000 compounds. Unglycosylated anthocyanidins are unstable and they are the first substrate to which the rose glucosyltransferase adds a glucoside. Only it adds it in such a way that the novel glucoside is unstable. So the glucosyltransferase adds a further glucoside thus creating a diglucoside. Thus gylcosylated, the anthocyanin is stable in roses, and gives the flower its hue.

Why is it that glycosylation in roses does not proceed in the same way as in other plants? The intricateness of colour – as of smell – evolved in plants for the purpose of pollination. Plants fast needed the aid of insects – and vice versa – not only to survive but also to spread, and the two species have been evolving in unison for millions of years. Besides their role in pollination, anthocyanins also protect plant cells from harmful UV radiation thanks to their antioxidative properties. Both these properties – colouration and protection – are of great practical interest. Anthocyanins can replace harmful dyes which have been banned, and their anti-oxidant properties – revealed by the apparent lower risk of chronic diseases such as cancer and cardiovascular disease associated with the consumption of fruit and vegetables – have been proved. In July 2005, a first account of anthocyanin synthesis via recombinant Escherichia coli was reported. Such an adventure should permit the synthesis of anthocyanin compounds on an industrial scale but also the creation of fresh ones with novel catalytic acitivities, singular structures and – who knows – perhaps the colour blue?

1. Ogata J., Kanno Y., Itoh Y., Tsugawa H., Suzuki M.
Plant biochemistry: anthocyanin biosynthesis in roses
Nature 435:757-758(2005)
PMID: 15944692

2. Yan Y., Chemler J., Huang L., Martens S., Koffas M.A.
Metabolic engineering of anthocyanin biosynthesis in Escherichia coli
Appl. Environ. Microbiol. 71:3617-3623(2005)
PMID: 16000769
Swiss-Prot cross references
Anthocyanidin 5,3-O-glucosyltransferase, Rosa hybrid cultivar: Q4R1I9
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Dans mon village d'origine, Bodio, la salle de cinéma Fax était encore ouverte au début des années 60. A l'époque, j'avais 8 ou 10 ans et j'adorais le cinéma. J'en faisais une grande consommation: tous les dimanches, notre curé projetait un film à la salle parroissale. Entrée 1 Frs. Je n'en manquais pas un. Aussi, lorsque je passais mes étés en Italie chez ma grande mère, j'allais aussi souvent que possible au cinéma du centre, l'Odéon, comme beaucoup d'autres enfants. Par contre, je ne pouvais pas profiter du Fax, juste en bas du quartier où j'habitais: l'entrée aux spectacles était permise seulement à partir de 16 ans, peu importe le genre de film. C'était le loi, il paraît. Il y avait une ou deux exceptions à Noël et nouvel an, mais c'êst tout. Ce n'était pas un endroit pour les enfants. Un des opérateurs, Monsieur Danilo, était un ami de mes parents. Un jour, il leur a proposé de me laisser regarder une pellicule depuis la salle de projection. La salle était presque entièrement occupée par trois énormes projecteurs à arc, avec cheminée d'aération. Un film nécessitait plusieurs bobines, qu'il fallait enchaîner en passant au vol la projection d'un projecteur à l'autre, sans que le publique ne remarque rien. Ceci était le travail principal de Danilo. Quelques petites fenètres donnaient sur la salle et sur l'écran. A travers une d'elles, perché sur un haut tabouret, j'ai savouré cette projection. C'était "La rose (bleu) de Bagdad". C'est là que j'ai connu Omar Kayyam, les assassins ou hashishins, le vieux de la montagne, ainsi que la rareté de la rose bleu. J'ai longtemps phantasmé sur moi même recevant l'admiration de toutes les filles, en plus d'une une fortune matérielle considérable, pour avoir découvert fortuitement, mais héroïquement, la rose bleu. Ceci a duré environ un lustre, jusqu'à ce que je la trouve offerte sur un catalogue de fleurs Hollandais. Quant à Omar Kayyam, peu après j'ai pu chiper "La vie et les amours d'Omar Kayyam" de la table de nuit de ma mère et depuis ce moment là je me suis considéré comme suffisamment renseigné sur le sujet, même si je n'ai pas encore lu un seul quatrin de lui. Le vieux de la montagne et la substance qu'il faisait consommer à ses assassins méritent d'être traités lors d'une prochaine issue du Protein Spotlight Update. Le cinéma Fax a fermé quelques années plus tard. On dit qu'il aurait été tué par la télévision dans tous les ménages.
Il est probable que j'aie vu d'autres films, perché sur ce tabouret dans la salle de projection, mais je me rapelle seulement de la rose bleu de Bagdad.

Posted by Anto Boverio on Monday 24 October 2005 10:21 CET

I loved it!!!! It was great 4 my Science Fair Project. Thanks for all the help!!!!!
-Shevah Rezaee

Posted by Shevah Rezaee on Wednesday 7 December 2005 0:31 CET

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