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UniProtKB/Swiss-Prot variant pages

UniProtKB/Swiss-Prot P51681: Variant p.Cys20Ser

C-C chemokine receptor type 5
Gene: CCR5
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Variant information Variant position: help 20 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Type of variant: help LB/B The variants are classified into three categories: LP/P, LB/B and US.
  • LP/P: likely pathogenic or pathogenic.
  • LB/B: likely benign or benign.
  • US: uncertain significance

Residue change: help From Cysteine (C) to Serine (S) at position 20 (C20S, p.Cys20Ser). Indicates the amino acid change of the variant. The one-letter and three-letter codes for amino acids used in UniProtKB/Swiss-Prot are those adopted by the commission on Biochemical Nomenclature of the IUPAC-IUB.
Physico-chemical properties: help Change from medium size and polar (C) to small size and polar (S) The physico-chemical property of the reference and variant residues and the change implicated.
BLOSUM score: help -1 The score within a Blosum matrix for the corresponding wild-type to variant amino acid change. The log-odds score measures the logarithm for the ratio of the likelihood of two amino acids appearing by chance. The Blosum62 substitution matrix is used. This substitution matrix contains scores for all possible exchanges of one amino acid with another:
  • Lowest score: -4 (low probability of substitution).
  • Highest score: 11 (high probability of substitution).
More information can be found on the following page

Polymorphism: help Variations in CCR5 are associated with resistance or susceptibility to immunodeficiency virus type 1 (resistance or susceptibility to HIV-1) [MIM:609423]. Variations in CCR5 gene also influence the rate of progression to AIDS after infection.Variations in CCR5 are associated with susceptibility to West Nile virus (WNV) infection [MIM:610379]. - Additional information on the polymorphism described.
Other resources: help Links to websites of interest for the variant.


Sequence information Variant position: help 20 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Protein sequence length: help 352 The length of the canonical sequence.
Location on the sequence: help MDYQVSSPIYDINYYTSEP C QKINVKQIAARLLPPLYSLV The residue change on the sequence. Unless the variant is located at the beginning or at the end of the protein sequence, both residues upstream (20) and downstream (20) of the variant will be shown.
Sequence annotation in neighborhood: help The regions or sites of interest surrounding the variant. In general the features listed are posttranslational modifications, binding sites, enzyme active sites, local secondary structure or other characteristics reported in the cited references. The "Sequence annotation in neighborhood" lines have a fixed format:
  • Type: the type of sequence feature.
  • Positions: endpoints of the sequence feature.
  • Description: contains additional information about the feature.
TypePositionsDescription
Chain 1 – 352 C-C chemokine receptor type 5
Topological domain 1 – 30 Extracellular
Modified residue 3 – 3 Sulfotyrosine
Modified residue 10 – 10 Sulfotyrosine
Modified residue 14 – 14 Sulfotyrosine
Modified residue 15 – 15 Sulfotyrosine
Glycosylation 6 – 6 O-linked (GalNAc...) serine
Glycosylation 7 – 7 O-linked (GalNAc...) serine
Disulfide bond 20 – 269
Mutagenesis 3 – 3 Y -> D. No sulfation and strongly decreases binding with CCL4 and CCL5; when associated with D-10; D-14 and D-15. Restores most CCL4 binding; when associated with D-10 and D-15.
Mutagenesis 3 – 3 Y -> F. No sulfation and strongly decreases binding with CCL4 and CCL5; when associated with F-10; F-14 and F-15.
Mutagenesis 6 – 6 S -> A. Strongly decreases CCL4 binding. No change in glycosylation status.
Mutagenesis 7 – 7 S -> A. No change in glycosylation status and binds CCL4 as efficiently as wild type.
Mutagenesis 10 – 10 Y -> F. No sulfation and greatly decreases binding of CCL4 and CCL5; when associated with F-3; F-14 and F-15. Small loss of sulfation; when associated with F-14 and F-15.
Mutagenesis 14 – 14 Y -> D. No sulfation and greatly decreased binding of CCL4 and CCL5; when associated with D-3; D-10 and D-14. No restoration of CCL4 binding; when associated with D-10 and D-15.
Mutagenesis 14 – 14 Y -> F. No sulfation and greatly decreases binding of CCL4 and CCL5; when associated with F-3; F-10; and F-15. Small loss of sulfation; when associated with F-10 and F-15.
Mutagenesis 15 – 15 Y -> D. No sulfation and greatly decreased binding of CCL4 and CCL5; when associated with D-3; D-10 and D-14. Restored most CCL4 binding; when associated with D-3 and D-10.
Mutagenesis 15 – 15 Y -> F. No sulfation and greatly decreases binding of CCL4 and CCL5; when associated with F-3; F-10 and F-14. Small loss of sulfation; when associated with F-10 and F-14.
Mutagenesis 20 – 20 C -> A. Decreases to 40% surface expression. No effect on conformational integrity. Disrupts binding of CCL4. Decreases cell HIV infection.
Turn 20 – 22



Literature citations
Novel alleles of the chemokine-receptor gene CCR5.
Carrington M.; Kissner T.; Gerrard B.; Ivanov S.; O'Brien S.J.; Dean M.;
Am. J. Hum. Genet. 61:1261-1267(1997)
Cited for: VARIANTS LEU-12; SER-20; SER-29; PHE-42; GLN-55; SER-60; VAL-73; GLN-223; LYS-228 DEL; VAL-301; VAL-335 AND PHE-339; ASSOCIATION WITH SUSCEPTIBILITY TO HIV-1;
Disclaimer: Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. They are not in any way intended to be used as a substitute for professional medical advice, diagnostic, treatment or care.