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UniProtKB/Swiss-Prot variant pages

UniProtKB/Swiss-Prot Q8N4C8: Variant p.Pro775Leu

Misshapen-like kinase 1
Gene: MINK1
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Variant information Variant position: help 775 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Type of variant: help LB/B The variants are classified into three categories: LP/P, LB/B and US.
  • LP/P: likely pathogenic or pathogenic.
  • LB/B: likely benign or benign.
  • US: uncertain significance

Residue change: help From Proline (P) to Leucine (L) at position 775 (P775L, p.Pro775Leu). Indicates the amino acid change of the variant. The one-letter and three-letter codes for amino acids used in UniProtKB/Swiss-Prot are those adopted by the commission on Biochemical Nomenclature of the IUPAC-IUB.
Physico-chemical properties: help Similar physico-chemical property. Both residues are medium size and hydrophobic. The physico-chemical property of the reference and variant residues and the change implicated.
BLOSUM score: help -3 The score within a Blosum matrix for the corresponding wild-type to variant amino acid change. The log-odds score measures the logarithm for the ratio of the likelihood of two amino acids appearing by chance. The Blosum62 substitution matrix is used. This substitution matrix contains scores for all possible exchanges of one amino acid with another:
  • Lowest score: -4 (low probability of substitution).
  • Highest score: 11 (high probability of substitution).
More information can be found on the following page

Other resources: help Links to websites of interest for the variant.


Sequence information Variant position: help 775 The position of the amino-acid change on the UniProtKB canonical protein sequence.
Protein sequence length: help 1332 The length of the canonical sequence.
Location on the sequence: help HGHLPQAGSLERNRVGVSSK P DSSPVLSPGNKAKPDDHRSR The residue change on the sequence. Unless the variant is located at the beginning or at the end of the protein sequence, both residues upstream (20) and downstream (20) of the variant will be shown.
Residue conservation: help The multiple alignment of the region surrounding the variant against various orthologous sequences.
Human                         HGHLPQAGSLE--RNRVGVSSKPDSSPVLSPGNKAKPDDHRSR

Mouse                         HGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHR

Rat                           HGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHR

Sequence annotation in neighborhood: help The regions or sites of interest surrounding the variant. In general the features listed are posttranslational modifications, binding sites, enzyme active sites, local secondary structure or other characteristics reported in the cited references. The "Sequence annotation in neighborhood" lines have a fixed format:
  • Type: the type of sequence feature.
  • Positions: endpoints of the sequence feature.
  • Description: contains additional information about the feature.
TypePositionsDescription
Chain 1 – 1332 Misshapen-like kinase 1
Region 395 – 887 Disordered
Compositional bias 766 – 780 Polar residues
Modified residue 763 – 763 Phosphoserine
Modified residue 777 – 777 Phosphoserine
Modified residue 778 – 778 Phosphoserine
Modified residue 782 – 782 Phosphoserine



Literature citations
Identification and functional characterization of a novel human misshapen/Nck interacting kinase-related kinase, hMINK beta.
Hu Y.; Leo C.; Yu S.; Huang B.C.; Wang H.; Shen M.; Luo Y.; Daniel-Issakani S.; Payan D.G.; Xu X.;
J. Biol. Chem. 279:54387-54397(2004)
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4); FUNCTION; SUBCELLULAR LOCATION; INTERACTION WITH NCK1; TISSUE SPECIFICITY; ALTERNATIVE SPLICING; AUTOPHOSPHORYLATION; VARIANTS ALA-771 AND LEU-775; The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
The MGC Project Team;
Genome Res. 14:2121-2127(2004)
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3); VARIANTS ALA-771 AND LEU-775; Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.
Daub H.; Olsen J.V.; Bairlein M.; Gnad F.; Oppermann F.S.; Korner R.; Greff Z.; Keri G.; Stemmann O.; Mann M.;
Mol. Cell 31:438-448(2008)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641; SER-763 AND SER-782; VARIANT [LARGE SCALE ANALYSIS] LEU-775; IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]; A quantitative atlas of mitotic phosphorylation.
Dephoure N.; Zhou C.; Villen J.; Beausoleil S.A.; Bakalarski C.E.; Elledge S.J.; Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763; SER-777; SER-778 AND SER-782; VARIANT [LARGE SCALE ANALYSIS] LEU-775; IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]; Large-scale proteomics analysis of the human kinome.
Oppermann F.S.; Gnad F.; Olsen J.V.; Hornberger R.; Greff Z.; Keri G.; Mann M.; Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-763; VARIANT [LARGE SCALE ANALYSIS] LEU-775; IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]; Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.
Mayya V.; Lundgren D.H.; Hwang S.-I.; Rezaul K.; Wu L.; Eng J.K.; Rodionov V.; Han D.K.;
Sci. Signal. 2:RA46-RA46(2009)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-778; VARIANT [LARGE SCALE ANALYSIS] LEU-775; IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]; Toward a comprehensive characterization of a human cancer cell phosphoproteome.
Zhou H.; Di Palma S.; Preisinger C.; Peng M.; Polat A.N.; Heck A.J.; Mohammed S.;
J. Proteome Res. 12:260-271(2013)
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641; SER-701; SER-754; SER-763 AND SER-778; VARIANT [LARGE SCALE ANALYSIS] LEU-775; IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]; Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.
Gauci S.; Helbig A.O.; Slijper M.; Krijgsveld J.; Heck A.J.; Mohammed S.;
Anal. Chem. 81:4493-4501(2009)
Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-775; IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS];
Disclaimer: Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. They are not in any way intended to be used as a substitute for professional medical advice, diagnostic, treatment or care.