Cellosaurus THP-1 (CVCL_0006)

Cell line name THP-1
Synonyms THP1; THP 1; THPI; THP-1(ATCC); Tohoku Hospital Pediatrics-1
Accession CVCL_0006
Resource Identification Initiative To cite this cell line use: THP-1 (RRID:CVCL_0006)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: MD Anderson Cell Lines Project.
Part of: RAS genetic alteration cell panel (ATCC TCP-1031).
Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Characteristics: Can differentiate from monocytes into macrophage-like cells upon stimulation with PMA.
Doubling time: 26 hours (PubMed=25984343); ~35-50 hours (DSMZ).
Microsatellite instability: Stable (MSS) (Sanger).
Sequence variation: Heterozygous for NRAS p.Gly12Asp (ATCC).
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep promoter analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Glycoproteome analysis by proteomics.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Derived from sampling site: Peripheral blood.
Disease Childhood acute monocytic leukemia (NCIt: C9163)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_5115 (A-THP-1); CVCL_X687 (BF24); CVCL_KB46 (CellSensor NFkB-bla THP-1); CVCL_KS29 (CellSensor SIE-bla THP-1); CVCL_KS30 (CellSensor TNFalpha-bla THP-1); CVCL_KZ70 (PathHunter THP-1 IkappaB Degradation); CVCL_IR08 (PSC-THP1); CVCL_DF52 (THP-1 NucLight Red); CVCL_4V22 (THP-1/ara-C); CVCL_0R21 (THP-1/DC-SIGN); CVCL_0R25 (THP-1(NCI)); CVCL_3426 (THP-1h); CVCL_3427 (THP-1l); CVCL_X588 (THP1-Blue ISG); CVCL_X589 (THP1-Blue ISG-KD-STING); CVCL_X585 (THP1-Blue NF-kappaB); CVCL_X598 (THP1-defASC); CVCL_5I76 (THP1-defCASP1); CVCL_X600 (THP1-defNLRP3); CVCL_X599 (THP1-Dual); CVCL_X587 (THP1-Lucia ISG); CVCL_X590 (THP1-Lucia NF-kappaB); CVCL_5I77 (THP1-NLRC4); CVCL_X601 (THP1-Null); CVCL_X582 (THP1-Xblue)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CLS; Cosmic-CLP; DSMZ; ECACC; ICLC; JCRB; KCLB; PubMed=11416159; PubMed=25877200; RCB; TKG

Markers:
AmelogeninX,Y
CSF1PO11,13
D13S31713 (AddexBio; ATCC; CLS; Cosmic-CLP; ECACC; JCRB; KCLB; PubMed=25877200)
8,13 (DSMZ; RCB; TKG)
D16S53911,12
D18S5113,14
D21S1130,31.2
D3S135815,17
D5S81811,12
D7S82010
D8S117910,14
FGA24,25
Penta D10,12
Penta E11,15
TH018,9.3 (AddexBio; ATCC; CLS; Cosmic-CLP; DSMZ; ECACC; ICLC; KCLB; PubMed=11416159; PubMed=25877200; RCB; TKG)
8,10 (JCRB)
TPOX8,11
vWA16 (AddexBio; ATCC; CLS; Cosmic-CLP; ECACC; ICLC; JCRB; KCLB; PubMed=11416159; PubMed=25877200)
16,17 (DSMZ; RCB; TKG)
Web pages https://en.wikipedia.org/wiki/THP-1_cell_line
http://www.proteinatlas.org/learn/cellines
https://strap.nci.nih.gov/celline_detail.php?sample_id=78
http://www.infarktforschung.de/macrophages_cell_lines.html
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/t/cell-lines-detail-444.html
http://tcpaportal.org/mclp/
Publications

PubMed=6970727; DOI=10.1002/ijc.2910260208
Tsuchiya S., Yamabe M., Yamaguchi Y., Kobayashi Y., Konno T., Tada K.
Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).
Int. J. Cancer 26:171-176(1980)

PubMed=6091813
Palumbo A., Minowada J., Erikson J., Croce C.M., Rovera G.
Lineage infidelity of a human myelogenous leukemia cell line.
Blood 64:1059-1063(1984)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x
Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=14671638; DOI=10.1038/sj.leu.2403236
Drexler H.G., Quentmeier H., MacLeod R.A.F.
Malignant hematopoietic cell lines: in vitro models for the study of MLL gene alterations.
Leukemia 18:227-232(2004)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Andersson A., Eden P., Lindgren D., Nilsson J., Lassen C., Heldrup J., Fontes M., Borg A., Mitelman F., Johansson B., Hoglund M., Fioretos T.
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=16408098; DOI=10.1038/sj.leu.2404081
Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)

PubMed=19377474; DOI=10.1038/ng.375
Suzuki H., Forrest A.R.R., van Nimwegen E., Daub C.O., Balwierz P.J., Irvine K.M., Lassmann T., Ravasi T., Hasegawa Y., de Hoon M.J.L., Katayama S., Schroder K., Carninci P., Tomaru Y., Kanamori-Katayama M., Kubosaki A., Akalin A., Ando Y., Arner E., Asada M., Asahara H., Bailey T., Bajic V.B., Bauer D., Beckhouse A.G., Bertin N., Bjorkegren J., Brombacher F., Bulger E., Chalk A.M., Chiba J., Cloonan N., Dawe A., Dostie J., Engstrom P.G., Essack M., Faulkner G.J., Fink J.L., Fredman D., Fujimori K., Furuno M., Gojobori T., Gough J., Grimmond S.M., Gustafsson M., Hashimoto M., Hashimoto T., Hatakeyama M., Heinzel S., Hide W., Hofmann O., Hornquist M., Huminiecki L., Ikeo K., Imamoto N., Inoue S., Inoue Y., Ishihara R., Iwayanagi T., Jacobsen A., Kaur M., Kawaji H., Kerr M.C., Kimura R., Kimura S., Kimura Y., Kitano H., Koga H., Kojima T., Kondo S., Konno T., Krogh A., Kruger A., Kumar A., Lenhard B., Lennartsson A., Lindow M., Lizio M., MacPherson C., Maeda N., Maher C.A., Maqungo M., Mar J., Matigian N.A., Matsuda H., Mattick J.S., Meier S., Miyamoto S., Miyamoto-Sato E., Nakabayashi K., Nakachi Y., Nakano M., Nygaard S., Okayama T., Okazaki Y., Okuda-Yabukami H., Orlando V., Otomo J., Pachkov M., Petrovsky N., Plessy C., Quackenbush J., Radovanovic A., Rehli M., Saito R., Sandelin A., Schmeier S., Schonbach C., Schwartz A.S., Semple C.A., Sera M., Severin J., Shirahige K., Simons C., St Laurent G., Suzuki M., Suzuki T., Sweet M.J., Taft R.J., Takeda S., Takenaka Y., Tan K., Taylor M.S., Teasdale R.D., Tegner J., Teichmann S., Valen E., Wahlestedt C., Waki K., Waterhouse A., Wells C.A., Winther O., Wu L., Yamaguchi K., Yanagawa H., Yasuda J., Zavolan M., Hume D.A., Arakawa T., Fukuda S., Imamura K., Kai C., Kaiho A., Kawashima T., Kawazu C., Kitazume Y., Kojima M., Miura H., Murakami K., Murata M., Ninomiya N., Nishiyori H., Noma S., Ogawa C., Sano T., Simon C., Tagami M., Takahashi Y., Kawai J., Hayashizaki Y.
The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line.
Nat. Genet. 41:553-562(2009)

PubMed=22645513; DOI=10.3389/fendo.2011.00089
Keuper M., Dzyakanchuk A., Amrein K.E., Wabitsch M., Fischer-Posovszky P.
THP-1 macrophages and SGBS adipocytes - a new human in vitro model system of inflamed adipose tissue.
Front. Endocrinol. 2:89-89(2011)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23955599; DOI=10.1038/ng.2731
Kon A., Shih L.-Y., Minamino M., Sanada M., Shiraishi Y., Nagata Y., Yoshida K., Okuno Y., Bando M., Nakato R., Ishikawa S., Sato-Otsubo A., Nagae G., Nishimoto A., Haferlach C., Nowak D., Sato Y., Alpermann T., Nagasaki M., Shimamura T., Tanaka H., Chiba K., Yamamoto R., Yamaguchi T., Otsu M., Obara N., Sakata-Yanagimoto M., Nakamaki T., Ishiyama K., Nolte F., Hofmann W.K., Miyawaki S., Chiba S., Mori H., Nakauchi H., Koeffler H.P., Aburatani H., Haferlach T., Shirahige K., Miyano S., Ogawa S.
Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.
Nat. Genet. 45:1232-1237(2013)

PubMed=25130606; DOI=10.1016/j.intimp.2014.08.002
Chanput W., Mes J.J., Wichers H.J.
THP-1 cell line: an in vitro cell model for immune modulation approach.
Int. Immunopharmacol. 23:37-45(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=28404994; DOI=10.1038/s41598-017-00828-y
Muller M.M., Lehmann R., Klassert T.E., Reifenstein S., Conrad T., Moore C., Kuhn A., Behnert A., Guthke R., Driesch D., Slevogt H.
Global analysis of glycoproteins identifies markers of endotoxin tolerant monocytes and GPR84 as a modulator of TNFalpha expression.
Sci. Rep. 7:838-838(2017)

Cross-references
Cell line collections AddexBio; C0003024/4956
ATCC; TIB-202
BCRC; 60430
BCRJ; 0234
CLS; 300356/p804_THP-1
DSMZ; ACC-16
ECACC; 88081201
ICLC; HTL97014
IZSLER; BS TCL 138
JCRB; JCRB0112
JCRB; JCRB0112.1
KCB; KCB 200549YJ
KCLB; 40202
NCBI_Iran; C563
NIH-ARP; 9942-142
RCB; RCB1189
TKG; TKG 0267
Cell line databases/resources CLDB; cl4513
CLDB; cl4514
CLDB; cl4515
CLDB; cl4517
CCLE; THP1_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE
CCRID; 3111C0001CCC000057
CCRID; 3111C0002000000058
CCRID; 3131C0001000700057
CCRID; 3142C0001000000114
Cosmic-CLP; 909771
GDSC; 909771
IGRhCellID; THP1
LINCS_HMS; 50861
LINCS_LDP; LCL-1072
Ontologies BTO; BTO:0001370
CLO; CLO_0009348
CLO; CLO_0050999
EFO; EFO_0001253
MCCL; MCC:0000461
Biological sample resources BioSample; SAMN03470795
BioSample; SAMN03473256
BioSample; SAMN03473419
Chemistry resources ChEMBL-Cells; CHEMBL3307574
ChEMBL-Targets; CHEMBL614245
Gene expression databases GEO; GSM236807
GEO; GSM236843
GEO; GSM482495
GEO; GSM887706
GEO; GSM888799
GEO; GSM1374962
GEO; GSM1446742
GEO; GSM1670543
Other Wikidata; Q7670282
Polymorphism and mutation databases Cosmic; 717684
Cosmic; 724818
Cosmic; 787480
Cosmic; 909771
Cosmic; 919122
Cosmic; 924051
Cosmic; 975303
Cosmic; 991556
Cosmic; 996316
Cosmic; 998748
Cosmic; 1012124
Cosmic; 1037666
Cosmic; 1070694
Cosmic; 1078722
Cosmic; 1089517
Cosmic; 1150901
Cosmic; 1176587
Cosmic; 1181604
Cosmic; 1187864
Cosmic; 1197931
Cosmic; 1278779
Cosmic; 1281356
Cosmic; 1319551
Cosmic; 1476425
Cosmic; 1519163
Cosmic; 1524842
Cosmic; 1601064
Cosmic; 2089655
Cosmic; 2131559
Cosmic; 2306241
Cosmic; 2392976
Proteomic databases PRIDE; PRD000345
PRIDE; PXD000328
PRIDE; PXD000953
PRIDE; PXD004034