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Cellosaurus U-937 (CVCL_0007)

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Cell line name U-937
Synonyms U937; U 937
Accession CVCL_0007
Resource Identification Initiative To cite this cell line use: U-937 (RRID:CVCL_0007)
Comments Group: Space-flown cell line (cellonaut).
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: LL-100 blood cancer cell line panel.
Characteristics: Can be induced to differentiate into macrophages by several factors such as phorbol ester (PMA) and 12-O-tetradecanoyl-13-phorbol acetate (TPA).
Doubling time: 95-100 hours (PubMed=178611); ~30-40 hours (DSMZ).
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Proteome analysis by 2D-DE/MS.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Anecdotal: Have been flown in space on shuttle flight STS-76 and on Shenzhou-8 to study growth and cytoskeletal changes in microgravity (PubMed=10352142; DOI=10.1016/j.actaastro.2013.06.007).
Discontinued: TKG; TKG 0279; probable.
Derived from sampling site: Pleural effusion.
Sequence variations PICALM-MLLT10 (CALM-AF10) gene fusion (PubMed=8643484; PubMed=25485619).
Hemizygous for PTEN p.Gly129fs*51 (c.387_388insCGCC) (PubMed=10966170; CCLE).
Homozygous for TP53 c.559+1G>A; splice donor mutation (PubMed=1571549; CCLE).
HLA typing Source: IPD-IMGT/HLA; 11507
Class II
HLA-DRDRB1*16:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0
East Asian, North2.39
East Asian, South0
South Asian0
European, North71.54
European, South26.06
Disease Adult acute monocytic leukemia (NCIt: C8263)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_3680 (EL 1)CVCL_2082 (JOSK-I)CVCL_8141 (JOSK-K)
CVCL_2083 (JOSK-M)CVCL_8142 (JOSK-S)CVCL_8442 (MOBS-1)
CVCL_L107 (PH [Human])CVCL_9997 (SAML-1)CVCL_6444 (SC)
CVCL_1775 (TUR)CVCL_2810 (U-937 cl1-14)CVCL_2811 (U-937 cl1-22)
CVCL_8765 (U-937 DE-4)CVCL_U631 (U-937 GTB)CVCL_WV56 (U-937 RERC)
CVCL_X000 (U-937-AG)CVCL_WV55 (U-937/CR)CVCL_VG84 (U-M)
CVCL_M769 (U1/HIV-1)CVCL_M770 (U38)CVCL_2Z95 (U937-DC-SIGN)
CVCL_4V23 (U937/ara-C)CVCL_Y170 (U937/clone21)CVCL_Y171 (U937/clone36)
CVCL_Y172 (U937/clone41)CVCL_Y173 (U937/clone49)CVCL_X608 (U937/HIV-2MS)
CVCL_Y174 (U937/UK110)CVCL_Y175 (U937/UK711)CVCL_8932 (U937(CD59-))
CVCL_8927 (U937(CD59+))CVCL_8466 (YAA)CVCL_8467 (YAP)
Sex of cell Male
Age at sampling 37Y
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CCRID; CLS; COG; DSMZ; ECACC; JCRB; KCLB; PubMed=11416159; PubMed=15637111; PubMed=25877200; RCB

Markers:
AmelogeninX
CSF1PO12 (AddexBio; ATCC; CCRID; CLS; COG; ECACC; JCRB; KCLB; PubMed=25877200; RCB)
10,12 (DSMZ; PubMed=15637111)
D2S133817,20
D3S135816
D5S81812 (AddexBio; ATCC; CCRID; CLS; COG; ECACC; JCRB; KCLB; PubMed=25877200; RCB)
10,12 (PubMed=15637111)
10,12,13 (DSMZ)
D7S8209,11
D8S117912,13
D13S31710,12
D16S53912
D18S5113,14
D19S43314,16
D21S1127,29 (CCRID; CLS; COG; PubMed=25877200)
27 (PubMed=15637111)
FGA22,25
Penta D12,13 (CLS; COG; PubMed=25877200)
13 (PubMed=15637111)
Penta E13
TH016,9.3 (AddexBio; ATCC; CCRID; CLS; COG; DSMZ; ECACC; JCRB_IFO50038; PubMed=11416159; PubMed=15637111; PubMed=25877200; RCB)
6,10 (JCRB_JCRB9021)
9.3 (KCLB)
TPOX8,11
vWA15 (ATCC; KCLB)
14,15 (AddexBio; CCRID; CLS; COG; DSMZ; ECACC; JCRB; PubMed=11416159; PubMed=15637111; PubMed=25877200; RCB)

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/U937_cells
http://www.cells-talk.com/index.php/page/copelibrary?key=U937
http://www.proteinatlas.org/learn/cellines
http://www.infarktforschung.de/macrophages_cell_lines.html
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/u/cell-lines-detail-315.html
http://www.nibsc.org/Default.aspx?pageid=597&id=0012&theme=default
http://www.cellresource.cn/fdetail.aspx?id=59
http://www.cellresource.cn/fdetail.aspx?id=627
http://www.cellresource.cn/fdetail.aspx?id=725
http://www.cellresource.cn/fdetail.aspx?id=846
Publications

DOI=10.1007/978-1-4757-1647-4_13
Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=178611; DOI=10.1002/ijc.2910170504
Sundstrom C., Nilsson K.
Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
Int. J. Cancer 17:565-577(1976)

PubMed=1083890; DOI=10.1084/jem.143.6.1528
Ralph P., Moore M.A., Nilsson K.
Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.
J. Exp. Med. 143:1528-1533(1976)

PubMed=6091813
Palumbo A., Minowada J., Erikson J., Croce C.M., Rovera G.
Lineage infidelity of a human myelogenous leukemia cell line.
Blood 64:1059-1063(1984)

PubMed=6582512; DOI=10.1073/pnas.81.2.568
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

DOI=10.1159/000415060
Morikawa S., Harada T., Katoh T.
Heterogeneity of cellular origins in human malignant lymphoma cell line derived from histo-monocytic lineage cells.
(In) Cellular, molecular, genetic approaches to immunodiagnosis and immunotherapy. 8th International conference on labeled antibodies, Tokyo, November 1985; Kano K., Mori S., Sugisaki T., Torisu M. (eds.); pp.373-380; Karger; Basel (1988)

PubMed=2140233; DOI=10.1111/j.1440-1827.1990.tb01549.x
Nakano A., Harada T., Morikawa S., Kato Y.
Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines.
Acta Pathol. Jpn. 40:107-115(1990)

DOI=10.1007/978-94-011-3550-4_20
Shinohara K., Kong Z.-L., Murakami H.
Characterization of a human derived macrophage-like cell line, U-M.
(In) Animal cell culture and production of biologicals; Sasaki R., Ikura K. (eds.); pp.181-186; Springer; Dordrecht (1991)

PubMed=1913629; DOI=10.1016/0304-3835(91)90051-I
Chang K.S.-S., Liu W.-T., Josephs S.F.
Regulation of cellular trans-activating activities in two different promonocytic leukemia cell lines.
Cancer Lett. 60:75-83(1991)

PubMed=1571549
Sugimoto K., Toyoshima H., Sakai R., Miyagawa K., Hagiwara K., Ishikawa F., Takaku F., Yazaki Y., Hirai H.
Frequent mutations in the p53 gene in human myeloid leukemia cell lines.
Blood 79:2378-2383(1992)

PubMed=8300570
Rubin E., Pantazis P., Bharti A., Toppmeyer D., Giovanella B., Kufe D.
Identification of a mutant human topoisomerase I with intact catalytic activity and resistance to 9-nitro-camptothecin.
J. Biol. Chem. 269:2433-2439(1994)

PubMed=8547074; DOI=10.1111/j.1365-2141.1995.tb05302.x
Siebert R., Willers C.P., Schramm A., Fossa A., Dresen I.M.G., Uppenkamp M., Nowrousian M.R., Seeber S., Opalka B.
Homozygous loss of the MTS1/p16 and MTS2/p15 genes in lymphoma and lymphoblastic leukaemia cell lines.
Br. J. Haematol. 91:350-354(1995)

PubMed=8558913
Morita S., Tsuchiya S., Fujie H., Itano M., Ohashi Y., Minegishi M., Imaizumi M., Endo M., Takano N., Konno T.
Cell surface c-kit receptors in human leukemia cell lines and pediatric leukemia: selective preservation of c-kit expression on megakaryoblastic cell lines during adaptation to in vitro culture.
Leukemia 10:102-105(1996)

PubMed=8558920
Dirks W.G., Zaborski M., Jager K., Challier C., Shiota M., Quentmeier H., Drexler H.G.
The (2;5)(p23;q35) translocation in cell lines derived from malignant lymphomas: absence of t(2;5) in Hodgkin-analogous cell lines.
Leukemia 10:142-149(1996)

PubMed=8643484; DOI=10.1073/pnas.93.10.4804
Dreyling M.H., Martinez-Climent J.A., Zheng M., Mao J., Rowley J.D., Bohlander S.K.
The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.
Proc. Natl. Acad. Sci. U.S.A. 93:4804-4809(1996)

PubMed=9371509
Saleem A., Ibrahim N., Patel M., Li X.G., Gupta E., Mendoza J., Pantazis P., Rubin E.H.
Mechanisms of resistance in a human cell line exposed to sequential topoisomerase poisoning.
Cancer Res. 57:5100-5106(1997)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x
Hosoya N., Hangaishi A., Ogawa S., Miyagawa K., Mitani K., Yazaki Y., Hirai H.
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=9685479; DOI=10.1093/nar/26.16.3651
Hultdin M., Gronlund E., Norrback K.-F., Eriksson-Lindstrom E., Just T., Roos G.
Telomere analysis by fluorescence in situ hybridization and flow cytometry.
Nucleic Acids Res. 26:3651-3656(1998)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x
Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10352142; DOI=10.1096/fasebj.13.9001.s23
Hatton J.P., Gaubert F., Lewis M.L., Darsel Y., Ohlmann P., Cazenave J.-P., Schmitt D.
The kinetics of translocation and cellular quantity of protein kinase C in human leukocytes are modified during spaceflight.
FASEB J. 13:S23-S33(1999)

PubMed=10676951; DOI=10.1038/35000501
Alizadeh A.A., Eisen M.B., Davis R.E., Ma C., Lossos I.S., Rosenwald A., Boldrick J.C., Sabet H., Tran T., Yu X., Powell J.I., Yang L., Marti G.E., Moore T., Hudson J. Jr., Lu L., Lewis D.B., Tibshirani R., Sherlock G., Chan W.C., Greiner T.C., Weisenburger D.D., Armitage J.O., Warnke R., Levy R., Wilson W., Grever M.R., Byrd J.C., Botstein D., Brown P.O., Staudt L.M.
Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling.
Nature 403:503-511(2000)

PubMed=10966170; DOI=10.1034/j.1600-0609.2000.90181.x
Aggerholm A., Gronbaek K., Guldberg P., Hokland P.
Mutational analysis of the tumour suppressor gene MMAC1/PTEN in malignant myeloid disorders.
Eur. J. Haematol. 65:109-113(2000)

PubMed=11701946; DOI=10.1159/000048774
Strefford J.C., Foot N.J., Chaplin T., Neat M.J., Oliver R.T.D., Young B.D., Jones L.K.
The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH.
Cytogenet. Cell Genet. 94:9-14(2001)

PubMed=12393283; DOI=10.1016/S0165-4608(02)00565-4
Lee J.-Y., Lee C.-H., Shim S.-H., Seo H.-K., Kyhm J.-H., Cho S., Cho Y.-H.
Molecular cytogenetic analysis of the monoblastic cell line U937 karyotype clarification by G-banding, whole chromosome painting, microdissection and reverse painting, and comparative genomic hybridization.
Cancer Genet. Cytogenet. 137:124-132(2002)

PubMed=15637111; DOI=10.1096/fj.04-3062fje
Parson W., Kirchebner R., Muhlmann R., Renner K., Kofler A., Schmidt S., Kofler R.
Cancer cell line identification by short tandem repeat profiling: power and limitations.
FASEB J. 19:434-436(2005)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Andersson A., Eden P., Lindgren D., Nilsson J., Lassen C., Heldrup J., Fontes M., Borg A., Mitelman F., Johansson B., Hoglund M., Fioretos T.
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=16408098; DOI=10.1038/sj.leu.2404081
Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)

PubMed=20801507; DOI=10.1016/j.leukres.2010.07.040
Minafra L., Di Cara G., Albanese N.N., Cancemi P.
Proteomic differentiation pattern in the U937 cell line.
Leuk. Res. 35:226-236(2011)

PubMed=21269460; DOI=10.1186/1752-0509-5-17
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.
Initial characterization of the human central proteome.
BMC Syst. Biol. 5:17-17(2011)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

DOI=10.1016/j.actaastro.2013.06.007
Paulsen K., Tauber S., Goelz N., Simmet D.M., Engeli S., Birlem M., Dumrese C., Karer A., Hunziker S., Biskup J., Konopasek S., Suh D., Hurlimann E., Signer C., Wang A., Sang C., Grote K.-H., Zhuang F.-Y., Ullrich O.
Severe disruption of the cytoskeleton and immunologically relevant surface molecules in a human macrophageal cell line in microgravity -- results of an in vitro experiment on board of the Shenzhou-8 space mission.
Acta Astronaut. 94:277-292(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5
Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H., Koeffler H.P.
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940-940(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x
Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A.F., Nagel S., Steube K., Uphoff C.C., Drexler H.G.
The LL-100 panel: 100 cell lines for blood cancer studies.
Sci. Rep. 9:8218-8218(2019)

Cross-references
Cell line collections AddexBio; C0003005/72
ATCC; CRL-1593 - Discontinued
ATCC; CRL-1593.2
BCRC; 60435
BCRJ; 0242
CCTCC; GDC0080
CLS; 300368/p474_U-937
DSMZ; ACC-5
ECACC; 85011440
ICLC; HTL94002
IZSLER; BS TCL 73
JCRB; IFO50038
JCRB; JCRB9021
KCB; KCB 200676YJ
KCLB; 21593.1
NCBI_Iran; C130
RCB; RCB1978 - Discontinued
TKG; TKG 0279
Cell line databases/resources CLDB; cl4597
CLDB; cl4599
CLDB; cl4600
CLDB; cl4601
CLDB; cl4602
CLDB; cl4603
CLDB; cl4604
CLDB; cl4605
CLDB; cl4606
CLDB; cl4608
CCLE; U937_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE
CCRID; 3111C0001CCC000059
CCRID; 3131C0001000700059
CCRID; 3131C0001000700159
CCRID; 3142C0001000000078
Cell_Model_Passport; SIDM01195
DepMap; ACH-000406
IPD-IMGT/HLA; 11507
LINCS_HMS; 50869
LINCS_LDP; LCL-1125
Lonza; 135
TOKU-E; 3382
Ontologies BTO; BTO:0001412
CLO; CLO_0009450
CLO; CLO_0009465
EFO; EFO_0001257
MCCL; MCC:0000470
MeSH; D020298
Biological sample resources BioSample; SAMN03472409
BioSample; SAMN03473173
BioSample; SAMN03473567
BioSample; SAMN10988180
Chemistry resources ChEMBL-Cells; CHEMBL3308006
ChEMBL-Targets; CHEMBL612794
PharmacoDB; U937_1624_2019
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-7721
ArrayExpress; E-MTAB-7722
GEO; GSE66854
GEO; GSM2020
GEO; GSM236809
GEO; GSM236845
GEO; GSM482491
GEO; GSM887724
GEO; GSM888818
GEO; GSM1028244
GEO; GSM1028245
GEO; GSM1374975
GEO; GSM1670560
Other Wikidata; Q7863610
Polymorphism and mutation databases Cosmic; 787483
Cosmic; 798666
Cosmic; 821044
Cosmic; 850382
Cosmic; 852000
Cosmic; 919123
Cosmic; 922162
Cosmic; 932770
Cosmic; 947364
Cosmic; 975307
Cosmic; 991558
Cosmic; 994178
Cosmic; 999781
Cosmic; 1012127
Cosmic; 1019837
Cosmic; 1067446
Cosmic; 1070690
Cosmic; 1078726
Cosmic; 1082516
Cosmic; 1086353
Cosmic; 1089523
Cosmic; 1107178
Cosmic; 1127260
Cosmic; 1150904
Cosmic; 1176581
Cosmic; 1181605
Cosmic; 1191692
Cosmic; 1465967
Cosmic; 1523828
Cosmic; 1524826
Cosmic; 1582388
Cosmic; 1601066
Cosmic; 1604865
Cosmic; 1995661
Cosmic; 2301570
Cosmic; 2361376
IARC_TP53; 2199
LiGeA; CCLE_826
Proteomic databases PRIDE; PXD001521
PRIDE; PXD001522
PRIDE; PXD001523
Entry history
Entry creation04-Apr-2012
Last entry updated06-Sep-2019
Version number31