Cell line name | SH-SY5Y | ||||||||||||||||||||||||||||||||||||||||||
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Synonyms | SHSY5Y; SHSY-5Y; SH-Sy5y; SK-SH-SY5Y; SY5Y; SH-SY5Y Parental | ||||||||||||||||||||||||||||||||||||||||||
Accession | CVCL_0019 | ||||||||||||||||||||||||||||||||||||||||||
Resource Identification Initiative | To cite this cell line use: SH-SY5Y (RRID:CVCL_0019) | ||||||||||||||||||||||||||||||||||||||||||
Comments | Problematic cell line: Partially contaminated. Some laboratories that are redistributing this cell line are in fact redistributing a contaminated cell line of mouse origin (PubMed=25182563). Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: ENCODE project common cell types; tier 3. From: Memorial Sloan Kettering Cancer Center; New York; USA. Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 810. Characteristics: Neuroblastic type (N-type) (PubMed=15720811). Characteristics: There seems to be differences in the retinoic acid (RA)-induced neuronal phenotype of SH-SY5Y cells from ATCC and ECACC. After 5 days of RA treatment, ECACC cells are slightly larger in size and contains significant amount of neuroblastic (N-type) cells and a small fraction of epithelial (S-type) cells (PubMed=18957096). Doubling time: >55 hours (DSMZ). Omics: Deep antibody staining analysis. Omics: Deep exome analysis. Omics: Deep proteome analysis. Omics: Deep tyrosine phosphoproteome analysis. Omics: Deep RNAseq analysis. Omics: GPI-anchored proteins analysis by proteomics. Omics: Mitochondrial proteome analysis by 2D-DE/MS. Omics: N-glycan profiling. Omics: SNP array analysis. Omics: Transcriptome analysis. Misspelling: SH-SY5; Occasionally. | ||||||||||||||||||||||||||||||||||||||||||
Sequence variations | ALK p.Phe1174Leu (c.3522C>A) (ClinVar=VCV000217852) (PubMed=28350380). | ||||||||||||||||||||||||||||||||||||||||||
Genome ancestry | Source: PubMed=30894373
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Disease | Neuroblastoma (NCIt: C3270) Neuroblastoma (ORDO: Orphanet_635) Derived from metastatic site: Bone marrow. | ||||||||||||||||||||||||||||||||||||||||||
Species of origin | Homo sapiens (Human)
(NCBI Taxonomy: 9606)
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Hierarchy | Parent: CVCL_0531 (SK-N-SH) Children:
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Sex of cell | Female | ||||||||||||||||||||||||||||||||||||||||||
Age at sampling | 4Y | ||||||||||||||||||||||||||||||||||||||||||
Category | Cancer cell line | ||||||||||||||||||||||||||||||||||||||||||
STR profile | Source(s): ATCC; CCRID; CLS; DSMZ; ECACC; KCLB Markers:
Run an STR similarity search on this cell line | ||||||||||||||||||||||||||||||||||||||||||
Web pages | https://en.wikipedia.org/wiki/SH-SY5Y http://www.proteinatlas.org/learn/cellines http://genome.ucsc.edu/ENCODE/protocols/cell/human/SH-SY5Y_Farnham_protocol.pdf http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-101.html https://www.mskcc.org/research-advantage/support/technology/tangible-material/human-neuroblastoma-cell-line-sh-sy5y https://www.phe-culturecollections.org.uk/media/114601/sh-sy5y-cell-line-profile.pdf http://www.cellresource.cn/fdetail.aspx?id=26 http://www.cellresource.cn/fdetail.aspx?id=665 http://www.cellresource.cn/fdetail.aspx?id=1133 http://www.cellresource.cn/fdetail.aspx?id=2174 http://www.cellresource.cn/fdetail.aspx?id=2201 | ||||||||||||||||||||||||||||||||||||||||||
Publications | PubMed=4748425 PubMed=6137586; DOI=10.1093/jnci/71.4.741 PubMed=3968181; DOI=10.1083/jcb.100.2.588 PubMed=2846152 PubMed=2535691 PubMed=1354203; DOI=10.1002/ijc.2910520116 PubMed=10630978; DOI=10.1667/0033-7587(2000)153[0062:FORIMI]2.0.CO;2 PubMed=16120276; DOI=10.1016/S1567-7249(01)00007-1 PubMed=15150091; DOI=10.1158/0008-5472.CAN-03-0809 PubMed=15720811; DOI=10.1593/neo.04310 PubMed=16822308; DOI=10.1186/1471-2407-6-177 PubMed=18957096; DOI=10.1186/1756-0500-1-95 PubMed=22213050; DOI=10.1002/ijc.27415 PubMed=22460905; DOI=10.1038/nature11003 PubMed=23421552; DOI=10.1089/ast.2012.0822 PubMed=24466371; DOI=10.1593/tlo.13544 PubMed=25182563; DOI=10.1007/s13577-014-0096-9 PubMed=25730103; DOI=10.1021/pr5011718 PubMed=25884760; DOI=10.1371/journal.pcbi.1004130 PubMed=26342562; DOI=10.1016/j.scr.2015.08.008 PubMed=26972028; DOI=10.1016/j.jprot.2016.03.008 PubMed=27141528; DOI=10.1016/j.dib.2016.04.001 PubMed=28118852; DOI=10.1186/s13024-017-0149-0 PubMed=28350380; DOI=10.1038/sdata.2017.33 PubMed=28601559; DOI=10.1016/j.cels.2017.05.009 PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747 PubMed=31068700; DOI=10.1038/s41586-019-1186-3 | ||||||||||||||||||||||||||||||||||||||||||
Cross-references | |||||||||||||||||||||||||||||||||||||||||||
Cell line collections | AddexBio; C0005004/63
ATCC; CRL-2266 BCRJ; 0223 CCTCC; GDC0210 CLS; 300154/p822_SH-SY5Y DSMZ; ACC-209 ECACC; 94030304 ICLC; HTL95013 IZSLER; BS TCL 232 KCB; KCB 2006107YJ KCLB; 22266 Kerafast; ECP004 NCBI_Iran; C611 | ||||||||||||||||||||||||||||||||||||||||||
Cell line databases/resources | CLDB; cl4287
CLDB; cl4288 CLDB; cl4289 CLDB; cl4290 CCLE; SHSY5Y_AUTONOMIC_GANGLIA CCRID; 3111C0001CCC000026 CCRID; 3131C0001000700097 CCRID; 3142C0001000000486 CCRID; 3142C0001000000964 CCRID; 3153C0001000000223 Cell_Model_Passport; SIDM01236 CGH-DB; 63-1 DepMap; ACH-001188 LINCS_LDP; LCL-2000 Lonza; 132 TOKU-E; 3118 | ||||||||||||||||||||||||||||||||||||||||||
Ontologies | BTO; BTO:0000793
CLO; CLO_0009015 EFO; EFO_0002717 MCCL; MCC:0000421 | ||||||||||||||||||||||||||||||||||||||||||
Biological sample resources | BioSample; SAMN10987717
ENCODE; ENCBS264AAA | ||||||||||||||||||||||||||||||||||||||||||
Chemistry resources | ChEMBL-Cells; CHEMBL3307740
ChEMBL-Targets; CHEMBL614910 PharmacoDB; SHSY5Y_1374_2019 | ||||||||||||||||||||||||||||||||||||||||||
Gene expression databases | GEO; GSM231608
GEO; GSM231609 GEO; GSM231610 GEO; GSM231611 GEO; GSM231612 GEO; GSM231613 GEO; GSM231614 GEO; GSM231615 GEO; GSM231616 GEO; GSM231617 GEO; GSM231618 GEO; GSM231619 GEO; GSM231620 GEO; GSM231621 GEO; GSM231622 GEO; GSM231623 GEO; GSM231624 GEO; GSM231625 GEO; GSM231626 GEO; GSM231627 GEO; GSM231628 GEO; GSM231629 GEO; GSM231630 GEO; GSM231631 GEO; GSM231632 GEO; GSM231633 GEO; GSM231634 GEO; GSM231635 GEO; GSM231636 GEO; GSM231637 GEO; GSM231638 GEO; GSM231639 GEO; GSM231640 GEO; GSM231641 GEO; GSM231642 GEO; GSM231643 GEO; GSM231644 GEO; GSM231645 GEO; GSM231646 GEO; GSM231647 GEO; GSM231648 GEO; GSM231649 GEO; GSM231650 GEO; GSM231651 GEO; GSM231652 GEO; GSM231653 GEO; GSM231654 GEO; GSM231655 GEO; GSM231656 GEO; GSM231657 GEO; GSM231658 GEO; GSM231659 GEO; GSM231660 GEO; GSM231661 GEO; GSM231662 GEO; GSM231663 GEO; GSM231664 GEO; GSM231665 GEO; GSM231666 GEO; GSM231667 GEO; GSM231668 GEO; GSM231669 GEO; GSM231670 GEO; GSM231671 GEO; GSM231672 GEO; GSM231673 GEO; GSM692874 GEO; GSM887570 GEO; GSM888653 GEO; GSM1622294 GEO; GSM2371253 GEO; GSM2394368 | ||||||||||||||||||||||||||||||||||||||||||
Metabolomic databases | MetaboLights; MTBLS455 | ||||||||||||||||||||||||||||||||||||||||||
Other | Wikidata; Q7390126 | ||||||||||||||||||||||||||||||||||||||||||
Polymorphism and mutation databases | Cosmic; 688084
Cosmic; 1019933 Cosmic; 1167410 Cosmic; 1212536 Cosmic; 2058109 Cosmic; 2239470 Cosmic; 2393641 Progenetix; CVCL_0019 | ||||||||||||||||||||||||||||||||||||||||||
Proteomic databases | PRIDE; PXD003105
PRIDE; PXD003914 PRIDE; PXD004452 PRIDE; PXD010005 PRIDE; PXD010027 | ||||||||||||||||||||||||||||||||||||||||||
Entry history | |||||||||||||||||||||||||||||||||||||||||||
Entry creation | 04-Apr-2012 | ||||||||||||||||||||||||||||||||||||||||||
Last entry update | 29-Oct-2020 | ||||||||||||||||||||||||||||||||||||||||||
Version number | 34 |