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Cellosaurus SH-SY5Y (CVCL_0019)

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Cell line name SH-SY5Y
Synonyms SH-Sy5y; SHSY5Y; SHSY-5Y; SK-SH-SY5Y; SY5Y; SH-SY5Y Parental
Accession CVCL_0019
Resource Identification Initiative To cite this cell line use: SH-SY5Y (RRID:CVCL_0019)
Comments Problematic cell line: Partially contaminated. Some laboratories that are redistributing this cell line are in fact redistributing a contaminated cell line of mouse origin (PubMed=25182563).
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: ENCODE project common cell types; tier 3.
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 810.
Characteristics: Neuroblastic type (N-type) (PubMed=15720811).
Characteristics: There seems to be differences in the retinoic acid (RA)-induced neuronal phenotype of SH-SY5Y cells from ATCC and ECACC. After 5 days of RA treatment, ECACC cells are slightly larger in size and contains significant amount of neuroblastic (N-type) cells and a small fraction of epithelial (S-type) cells (PubMed=18957096).
Doubling time: >55 hours (DSMZ).
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: Deep tyrosine phosphoproteome analysis.
Omics: Deep RNAseq analysis.
Omics: GPI-anchored proteins analysis by proteomics.
Omics: Mitochondrial proteome analysis by 2D-DE/MS.
Omics: N-glycan profiling.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: SH-SY5; Occasionally.
Sequence variations Mutation; HGNC; 427; ALK; Simple; p.Phe1174Leu (c.3522C>A); ClinVar=VCV000217852; Zygosity=Unspecified (PubMed=28350380).
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American1.17
East Asian, North0.35
East Asian, South0.1
South Asian0.08
European, North69.09
European, South29.2
Disease Neuroblastoma (NCIt: C3270)
Neuroblastoma (ORDO: Orphanet_635)
Derived from metastatic site: Bone marrow.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0531 (SK-N-SH)
CVCL_5I53 (SH-SY5Y-HFE C282Y)CVCL_5I54 (SH-SY5Y-HFE H63D)CVCL_UJ96 (SH-SY5Y-pLNCX2 empty vector)
CVCL_0523 (SH-SY5Y-SPA4CT)CVCL_UJ94 (SH-SY5Y-TrkA, clone P23A)CVCL_UJ95 (SH-SY5Y-TrkB, clone BR6)
Sex of cell Female
Age at sampling 4Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS; DSMZ; ECACC; Genomics_Center_BCF_Technion; KCLB

Penta D10,12
Penta E7,11

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/SH-SY5Y

Biedler J.L., Helson L., Spengler B.A.
Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.
Cancer Res. 33:2643-2652(1973)

PubMed=6137586; DOI=10.1093/jnci/71.4.741
Ross R.A., Spengler B.A., Biedler J.L.
Coordinate morphological and biochemical interconversion of human neuroblastoma cells.
J. Natl. Cancer Inst. 71:741-747(1983)

PubMed=3968181; DOI=10.1083/jcb.100.2.588
Meyers M.B., Spengler B.A., Chang T.-D., Melera P.W., Biedler J.L.
Gene amplification-associated cytogenetic aberrations and protein changes in vincristine-resistant Chinese hamster, mouse, and human cells.
J. Cell Biol. 100:588-597(1985)

Preis P.N., Saya H., Nadasdi L., Hochhaus G., Levin V., Sadee W.
Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A.
Cancer Res. 48:6530-6534(1988)

Ciccarone V., Spengler B.A., Meyers M.B., Biedler J.L., Ross R.A.
Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.
Cancer Res. 49:219-225(1989)

PubMed=1354203; DOI=10.1002/ijc.2910520116
Gross N., Favre S., Beck D., Meyer M.
Differentiation-related expression of adhesion molecules and receptors on human neuroblastoma tissues, cell lines and variants.
Int. J. Cancer 52:85-91(1992)

Xu Y.-Y., Wands J.R., de la Monte S.M.
Characterization of thread proteins expressed in neuroectodermal tumors.
Cancer Res. 53:3823-3829(1993)

PubMed=10630978; DOI=10.1667/0033-7587(2000)153[0062:FORIMI]2.0.CO;2
Akudugu J.M., Slabbert J.P., Serafin A., Bohm L.
Frequency of radiation-induced micronuclei in neuronal cells does not correlate with clonogenic survival.
Radiat. Res. 153:62-67(2000)

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=16120276; DOI=10.1016/S1567-7249(01)00007-1
Scheffler N.K., Miller S.W., Carroll A.K., Anderson C., Davis R.E., Ghosh S.S., Gibson B.W.
Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from an SH-SY5Y neuroblastoma cell line.
Mitochondrion 1:161-179(2001)

PubMed=14676279; DOI=10.1242/jcs.00834
Goldschneider D., Blanc E., Raguenez G., Barrois M., Legrand A., Le Roux G., Haddada H., Benard J., Douc-Rasy S.
Differential response of p53 target genes to p73 overexpression in SH-SY5Y neuroblastoma cell line.
J. Cell Sci. 117:293-301(2004)

PubMed=15150091; DOI=10.1158/0008-5472.CAN-03-0809
Schaefer K.-L., Brachwitz K., Wai D.H., Braun Y., Diallo R., Korsching E., Eisenacher M., Voss R., van Valen F., Baer C., Selle B., Spahn L., Liao S.-K., Lee K.A.W., Hogendoorn P.C.W., Reifenberger G., Gabbert H.E., Poremba C.
Expression profiling of t(12;22) positive clear cell sarcoma of soft tissue cell lines reveals characteristic up-regulation of potential new marker genes including ERBB3.
Cancer Res. 64:3395-3405(2004)

PubMed=15720811; DOI=10.1593/neo.04310
Walton J.D., Kattan D.R., Thomas S.K., Spengler B.A., Guo H.-F., Biedler J.L., Cheung N.-K.V., Ross R.A.
Characteristics of stem cells from human neuroblastoma cell lines and in tumors.
Neoplasia 6:838-845(2004)

PubMed=16822308; DOI=10.1186/1471-2407-6-177
Dam V., Morgan B.T., Mazanek P., Hogarty M.D.
Mutations in PIK3CA are infrequent in neuroblastoma.
BMC Cancer 6:177.1-177.10(2006)

PubMed=18957096; DOI=10.1186/1756-0500-1-95
Nishida Y., Adati N., Ozawa R., Maeda A., Sakaki Y., Takeda T.
Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y.
BMC Res. Notes 1:95.1-95.11(2008)

PubMed=20655465; DOI=10.1016/j.cell.2010.06.004
Holzel M., Huang S., Koster J., Ora I., Lakeman A., Caron H., Nijkamp W., Xie J., Callens T., Asgharzadeh S., Seeger R.C., Messiaen L., Versteeg R., Bernards R.
NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.
Cell 142:218-229(2010)

PubMed=22213050; DOI=10.1002/ijc.27415
Gawecka J.E., Geerts D., Koster J., Caliva M.J., Sulzmaier F.J., Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Int. J. Cancer 131:1556-1568(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23421552; DOI=10.1089/ast.2012.0822
Zhang Y.-Q., Wang H.-B., Lai C.-J., Wang L., Deng Y.-L.
Comparative proteomic analysis of human SH-SY5Y neuroblastoma cells under simulated microgravity.
Astrobiology 13:143-150(2013)

PubMed=24466371; DOI=10.1593/tlo.13544
Loschmann N., Michaelis M., Rothweiler F., Zehner R., Cinatl J., Voges Y., Sharifi M., Riecken K., Meyer J., von Deimling A., Fichtner I., Ghafourian T., Westermann F., Cinatl J. Jr.
Testing of SNS-032 in a panel of human neuroblastoma cell lines with acquired resistance to a broad range of drugs.
Transl. Oncol. 6:685-696(2013)

PubMed=25182563; DOI=10.1007/s13577-014-0096-9
Jiang T., Wang H.-L.
Identification of cross-contamination in SH-SY5Y cell line.
Hum. Cell 27:176-178(2014)

PubMed=25730103; DOI=10.1021/pr5011718
Hu Y., Mayampurath A., Khan S., Cohen J.K., Mechref Y., Volchenboum S.L.
N-linked glycan profiling in neuroblastoma cell lines.
J. Proteome Res. 14:2074-2081(2015)

PubMed=25884760; DOI=10.1371/journal.pcbi.1004130
Palacios-Moreno J., Foltz L., Guo A.-L., Stokes M.P., Kuehn E.D., George L., Comb M.J., Grimes M.L.
Neuroblastoma tyrosine kinase signaling networks involve FYN and LYN in endosomes and lipid rafts.
PLoS Comput. Biol. 11:E1004130-E1004130(2015)

PubMed=26342562; DOI=10.1016/j.scr.2015.08.008
Ross R.A., Walton J.D., Han D., Guo H.-F., Cheung N.-K.V.
A distinct gene expression signature characterizes human neuroblastoma cancer stem cells.
Stem Cell Res. 15:419-426(2015)

PubMed=26972028; DOI=10.1016/j.jprot.2016.03.008
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Identification of glycosylphosphatidylinositol-anchored proteins and omega-sites using TiO2-based affinity purification followed by hydrogen fluoride treatment.
J. Proteomics 139:77-83(2016)

PubMed=27141528; DOI=10.1016/j.dib.2016.04.001
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Data for identification of GPI-anchored peptides and omega-sites in cancer cell lines.
Data Brief 7:1302-1305(2016)

PubMed=28118852; DOI=10.1186/s13024-017-0149-0
Xicoy H., Wieringa B., Martens G.J.M.
The SH-SY5Y cell line in Parkinson's disease research: a systematic review.
Mol. Neurodegener. 12:10.1-10.11(2017)

PubMed=28350380; DOI=10.1038/sdata.2017.33
Harenza J.L., Diamond M.A., Adams R.N., Song M.M., Davidson H.L., Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033-170033(2017)

PubMed=28601559; DOI=10.1016/j.cels.2017.05.009
Bekker-Jensen D.B., Kelstrup C.D., Batth T.S., Larsen S.C., Haldrup C., Bramsen J.B., Sorensen K.D., Hoyer S., Orntoft T.F., Andersen C.L., Nielsen M.L., Olsen J.V.
An optimized shotgun strategy for the rapid generation of comprehensive human proteomes.
Cell Syst. 4:587-599.e4(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cell line collections Abcam; ab275475
AddexBio; C0005004/63
ATCC; CRL-2266
BCRJ; 0223
CLS; 300154/p822_SH-SY5Y
ECACC; 94030304
ICLC; HTL95013
KCB; KCB 2006107YJ
KCLB; 22266
Kerafast; ECP004
NCBI_Iran; C611
Cell line databases/resources CLO; CLO_0009015
MCCL; MCC:0000421
CLDB; cl4287
CLDB; cl4288
CLDB; cl4289
CLDB; cl4290
CCRID; 1101HUM-PUMC000026
Cell_Model_Passport; SIDM01236
CGH-DB; 63-1
DepMap; ACH-001188
Lonza; 132
TOKU-E; 3118
Anatomy/cell type resources BTO; BTO:0000793
Biological sample resources BioSample; SAMN10987717
Chemistry resources ChEMBL-Cells; CHEMBL3307740
ChEMBL-Targets; CHEMBL614910
PharmacoDB; SHSY5Y_1374_2019
Encyclopedic resources Wikidata; Q7390126
Experimental variables resources EFO; EFO_0002717
Gene expression databases GEO; GSM231608
GEO; GSM231609
GEO; GSM231610
GEO; GSM231611
GEO; GSM231612
GEO; GSM231613
GEO; GSM231614
GEO; GSM231615
GEO; GSM231616
GEO; GSM231617
GEO; GSM231618
GEO; GSM231619
GEO; GSM231620
GEO; GSM231621
GEO; GSM231622
GEO; GSM231623
GEO; GSM231624
GEO; GSM231625
GEO; GSM231626
GEO; GSM231627
GEO; GSM231628
GEO; GSM231629
GEO; GSM231630
GEO; GSM231631
GEO; GSM231632
GEO; GSM231633
GEO; GSM231634
GEO; GSM231635
GEO; GSM231636
GEO; GSM231637
GEO; GSM231638
GEO; GSM231639
GEO; GSM231640
GEO; GSM231641
GEO; GSM231642
GEO; GSM231643
GEO; GSM231644
GEO; GSM231645
GEO; GSM231646
GEO; GSM231647
GEO; GSM231648
GEO; GSM231649
GEO; GSM231650
GEO; GSM231651
GEO; GSM231652
GEO; GSM231653
GEO; GSM231654
GEO; GSM231655
GEO; GSM231656
GEO; GSM231657
GEO; GSM231658
GEO; GSM231659
GEO; GSM231660
GEO; GSM231661
GEO; GSM231662
GEO; GSM231663
GEO; GSM231664
GEO; GSM231665
GEO; GSM231666
GEO; GSM231667
GEO; GSM231668
GEO; GSM231669
GEO; GSM231670
GEO; GSM231671
GEO; GSM231672
GEO; GSM231673
GEO; GSM692874
GEO; GSM887570
GEO; GSM888653
GEO; GSM1622294
GEO; GSM2371253
GEO; GSM2394368
Metabolomic databases MetaboLights; MTBLS455
Polymorphism and mutation databases Cosmic; 688084
Cosmic; 1019933
Cosmic; 1167410
Cosmic; 1212536
Cosmic; 2058109
Cosmic; 2239470
Cosmic; 2393641
IARC_TP53; 23599
Progenetix; CVCL_0019
Proteomic databases PRIDE; PXD003105
PRIDE; PXD003914
PRIDE; PXD004452
PRIDE; PXD010005
PRIDE; PXD010027
Entry history
Entry creation04-Apr-2012
Last entry update16-Dec-2021
Version number37