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Cellosaurus SH-SY5Y (CVCL_0019)

Cell line name SH-SY5Y
Synonyms SHSY5Y; SHSY-5Y; SH-Sy5y; SK-SH-SY5Y; SY5Y
Accession CVCL_0019
Resource Identification Initiative To cite this cell line use: SH-SY5Y (RRID:CVCL_0019)
Comments Problematic cell line: Partially contaminated. Some laboratories that are redistributing this cell line are in fact redistributing a contaminated cell line of mouse origin (PubMed=25182563).
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: ENCODE project common cell types; tier 3.
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 810.
Characteristics: Neuroblastic type (N-type) (PubMed=15720811).
Doubling time: >55 hours (DSMZ).
Omics: Deep antibody staining analysis.
Omics: Deep RNAseq analysis.
Omics: GPI-anchored proteins analysis by proteomics.
Omics: Mitochondrial proteome analysis by 2D-DE/MS.
Omics: N-glycan profiling.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: SH-SY5; Occasionally.
Sequence variations ALK p.Phe1174Leu (c.3522C>A) (PubMed=28350380).
Disease Neuroblastoma (NCIt: C3270)
Derived from metastatic site: Bone marrow.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0531 (SK-N-SH)
CVCL_U924 (SH-SY5Y Rho0 64/5)CVCL_5I52 (SH-SY5Y-HFE)CVCL_5I53 (SH-SY5Y-HFE C282Y)
Sex of cell Female
Age at sampling 4Y
Category Cancer cell line
STR profile Source(s): ATCC; CLS; DSMZ; ECACC; KCLB

Penta D10,12
Penta E7,11
Web pages https://en.wikipedia.org/wiki/SH-SY5Y

Biedler J.L., Helson L., Spengler B.A.
Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.
Cancer Res. 33:2643-2652(1973)

PubMed=6137586; DOI=10.1093/jnci/71.4.741
Ross R.A., Spengler B.A., Biedler J.L.
Coordinate morphological and biochemical interconversion of human neuroblastoma cells.
J. Natl. Cancer Inst. 71:741-747(1983)

Preis P.N., Saya H., Nadasdi L., Hochhaus G., Levin V., Sadee W.
Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A.
Cancer Res. 48:6530-6534(1988)

Ciccarone V., Spengler B.A., Meyers M.B., Biedler J.L., Ross R.A.
Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.
Cancer Res. 49:219-225(1989)

PubMed=1354203; DOI=10.1002/ijc.2910520116
Gross N., Favre S., Beck D., Meyer M.
Differentiation-related expression of adhesion molecules and receptors on human neuroblastoma tissues, cell lines and variants.
Int. J. Cancer 52:85-91(1992)

PubMed=16120276; DOI=10.1016/S1567-7249(01)00007-1
Scheffler N.K., Miller S.W., Carroll A.K., Anderson C., Davis R.E., Ghosh S.S., Gibson B.W.
Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from an SH-SY5Y neuroblastoma cell line.
Mitochondrion 1:161-179(2001)

PubMed=15150091; DOI=10.1158/0008-5472.CAN-03-0809
Schaefer K.-L., Brachwitz K., Wai D.H., Braun Y., Diallo R., Korsching E., Eisenacher M., Voss R., van Valen F., Baer C., Selle B., Spahn L., Liao S.-K., Lee K.A.W., Hogendoorn P.C.W., Reifenberger G., Gabbert H.E., Poremba C.
Expression profiling of t(12;22) positive clear cell sarcoma of soft tissue cell lines reveals characteristic up-regulation of potential new marker genes including ERBB3.
Cancer Res. 64:3395-3405(2004)

PubMed=15720811; DOI=10.1593/neo.04310
Walton J.D., Kattan D.R., Thomas S.K., Spengler B.A., Guo H.-F., Biedler J.L., Cheung N.-K.V., Ross R.A.
Characteristics of stem cells from human neuroblastoma cell lines and in tumors.
Neoplasia 6:838-845(2004)

PubMed=16822308; DOI=10.1186/1471-2407-6-177
Dam V., Morgan B.T., Mazanek P., Hogarty M.D.
Mutations in PIK3CA are infrequent in neuroblastoma.
BMC Cancer 6:177-177(2006)

PubMed=22213050; DOI=10.1002/ijc.27415
Gawecka J.E., Geerts D., Koster J., Caliva M.J., Sulzmaier F.J., Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Int. J. Cancer 131:1556-1568(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24466371; DOI=10.1593/tlo.13544
Loschmann N., Michaelis M., Rothweiler F., Zehner R., Cinatl J., Voges Y., Sharifi M., Riecken K., Meyer J., von Deimling A., Fichtner I., Ghafourian T., Westermann F., Cinatl J. Jr.
Testing of SNS-032 in a panel of human neuroblastoma cell lines with acquired resistance to a broad range of drugs.
Transl. Oncol. 6:685-696(2013)

PubMed=25182563; DOI=10.1007/s13577-014-0096-9
Jiang T., Wang H.-L.
Identification of cross-contamination in SH-SY5Y cell line.
Hum. Cell 27:176-178(2014)

PubMed=25730103; DOI=10.1021/pr5011718
Hu Y., Mayampurath A., Khan S., Cohen J.K., Mechref Y., Volchenboum S.L.
N-linked glycan profiling in neuroblastoma cell lines.
J. Proteome Res. 14:2074-2081(2015)

PubMed=26972028; DOI=10.1016/j.jprot.2016.03.008
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Identification of glycosylphosphatidylinositol-anchored proteins and omega-sites using TiO2-based affinity purification followed by hydrogen fluoride treatment.
J. Proteomics 139:77-83(2016)

PubMed=27141528; DOI=10.1016/j.dib.2016.04.001
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Data for identification of GPI-anchored peptides and omega-sites in cancer cell lines.
Data Brief 7:1302-1305(2016)

PubMed=28118852; DOI=10.1186/s13024-017-0149-0
Xicoy H., Wieringa B., Martens G.J.M.
The SH-SY5Y cell line in Parkinson's disease research: a systematic review.
Mol. Neurodegener. 12:10-10(2017)

PubMed=28350380; DOI=10.1038/sdata.2017.33
Harenza J.L., Diamond M.A., Adams R.N., Song M.M., Davidson H.L., Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033-170033(2017)

Cell line collections AddexBio; C0005004/63
ATCC; CRL-2266
BCRJ; 0223
CLS; 300154/p822_SH-SY5Y
ECACC; 94030304
ICLC; HTL95013
KCB; KCB 2006107YJ
KCLB; 22266
NCBI_Iran; C611
Cell line databases/resources CLDB; cl4287
CLDB; cl4288
CLDB; cl4289
CLDB; cl4290
CCRID; 3111C0001CCC000026
CCRID; 3131C0001000700097
CCRID; 3142C0001000000486
CGH-DB; 63-1
Lonza; 132
TOKU-E; 3118
Ontologies BTO; BTO:0000793
CLO; CLO_0009015
EFO; EFO_0002717
MCCL; MCC:0000421
Biological sample resources ENCODE; ENCBS264AAA
Chemistry resources ChEMBL-Cells; CHEMBL3307740
ChEMBL-Targets; CHEMBL614910
Gene expression databases GEO; GSM692874
GEO; GSM887570
GEO; GSM888653
GEO; GSM1622294
GEO; GSM2371253
GEO; GSM2394368
Other Wikidata; Q7390126
Polymorphism and mutation databases Cosmic; 688084
Cosmic; 1019933
Cosmic; 1167410
Cosmic; 1212536
Cosmic; 2058109
Cosmic; 2239470
Cosmic; 2393641
Proteomic databases PRIDE; PXD003105
PRIDE; PXD003914