Cellosaurus U-251MG (CVCL_0021)

Cell line name U-251MG
Synonyms U-251 MG; U-251-MG; U-251_MG; U251-MG; U251MG; U-251; U251; U251n; U251N; 251 MG; 251MG
Accession CVCL_0021
Resource Identification Initiative To cite this cell line use: U-251MG (RRID:CVCL_0021)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: JFCR39 cancer cell line panel.
Part of: MD Anderson Cell Lines Project.
Part of: NCI60 cancer cell line panel.
Doubling time: 24 hours (PubMed=25984343); 23.8 hours (NCI-DTP).
Microsatellite instability: Stable (MSS) (Sanger).
Sequence variation: Has no IDH1 mutation (PubMed=19435942).
Omics: Array-based CGH.
Omics: Cell surface proteome.
Omics: CNV analysis.
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Fluorescence phenotype profiling.
Omics: lncRNA expression profiling.
Omics: Metabolome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Astrocytoma (NCIt: C60781)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_2864 (B2-17); CVCL_2800 (KNS-89); CVCL_0535 (SNB-19); CVCL_B325 (TK-1 [Human astrocytoma]); CVCL_2809 (U-251MG (KO)); CVCL_5G63 (U-251MG AgCl1); CVCL_5G56 (U-251MG sp); CVCL_J269 (U-251MG-Luc); CVCL_2219 (U-373MG ATCC); CVCL_1G29 (U251-TR3)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): CLS; Cosmic-CLP; ECACC; JCRB; PubMed=19372543; PubMed=22570425; PubMed=24810477; PubMed=27582061

Markers:
AmelogeninX,Y
X (PubMed=24810477_some_subclones)
CSF1PO11,12 (CLS; Cosmic-CLP; ECACC; JCRB; PubMed=22570425; PubMed=27582061)
12,13 (PubMed=19372543)
D13S31710,11
D16S53912
D18S5113,15
13 (CLS; PubMed=22570425; PubMed=24810477_some_subclones)
D19S43313,15
D21S1129,30
29 (PubMed=22570425; PubMed=24810477_some_subclones)
D2S133824 (CLS)
22,24 (PubMed=27582061)
D3S135816,17
D5S81811,12
11 (PubMed=24810477_some_subclones)
D7S82010,12
D8S117913,15
FGA21,25
20,21,25 (PubMed=24810477_some_subclones)
Penta D10,12 (CLS; PubMed=27582061)
12 (PubMed=22570425)
Penta E7,10 (CLS)
7 (PubMed=22570425; PubMed=27582061)
TH019.3
TPOX8
vWA16,18
Web pages https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm
https://dtp.cancer.gov/discovery_development/nci-60/u251_snb-19.htm
https://strap.nci.nih.gov/celline_detail.php?sample_id=57
https://www.kerafast.com/product/1790/u-251-mg-glioblastoma-cell-line
http://www.proteinatlas.org/learn/cellines
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/u/cell-lines-detail-313.html
http://tcpaportal.org/mclp/
Publications

PubMed=4134536; DOI=10.1002/ijc.2910120215
Westermark B.
The deficient density-dependent growth control of human malignant glioma cells and virus-transformed glia-like cells in culture.
Int. J. Cancer 12:438-451(1973)

PubMed=4369403; DOI=10.1002/1097-0142(197408)34:2<274::AID-CNCR2820340209>3.0.CO;2-4
Wahlstrom T., Linder E., Saksela E., Westermark B.
Tumor-specific membrane antigens in established cell lines from gliomas.
Cancer 34:274-279(1974)

DOI=10.1007/978-1-4757-1647-4_7
Ponten J.
Neoplastic human glia cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.175-206; Springer; New York (1975)

PubMed=608846; DOI=10.1111/j.1601-5223.1978.tb01267.x
Mark J., Westermark B., Ponten J., Hugosson R.
Banding patterns in human glioma cell lines.
Hereditas 87:243-260(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=6260907; DOI=10.1097/00005072-198105000-00001
Bigner D.D., Bigner S.H., Ponten J., Westermark B., Mahaley M.S. Jr., Ruoslahti E., Herschman H., Eng L.F., Wikstrand C.J.
Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas.
J. Neuropathol. Exp. Neurol. 40:201-229(1981)

PubMed=6582512; DOI=10.1073/pnas.81.2.568
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=2041050; DOI=10.1093/jnci/83.11.757
Monks A., Scudiero D.A., Skehan P., Shoemaker R.H., Paull K., Vistica D.T., Hose C., Langley J., Cronise P., Vaigro-Wolff A., Gray-Goodrich M., Campbell H., Mayo J., Boyd M.
Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.
J. Natl. Cancer Inst. 83:757-766(1991)

DOI=10.1016/B978-0-12-333530-2.50005-8
Nister M., Westermark B.
Human glioma cell lines.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.17-42; Academic Press; New York (1994)

PubMed=9220028; DOI=10.1016/S0303-7207(97)00080-4
Sharif T.R., Luo W., Sharif M.
Functional expression of bombesin receptor in most adult and pediatric human glioblastoma cell lines; role in mitogenesis and in stimulating the mitogen-activated protein kinase pathway.
Mol. Cell. Endocrinol. 130:119-130(1997)

PubMed=10700174; DOI=10.1038/73432
Ross D.T., Scherf U., Eisen M.B., Perou C.M., Rees C., Spellman P., Iyer V., Jeffrey S.S., Van de Rijn M., Waltham M., Pergamenschikov A., Lee J.C.F., Lashkari D., Shalon D., Myers T.G., Weinstein J.N., Botstein D., Brown P.O.
Systematic variation in gene expression patterns in human cancer cell lines.
Nat. Genet. 24:227-235(2000)

PubMed=16232199; DOI=10.1111/j.1349-7006.2005.00099.x
Saigusa K., Hashimoto N., Tsuda H., Yokoi S., Maruno M., Yoshimine T., Aoyagi M., Ohno K., Imoto I., Inazawa J.
Overexpressed Skp2 within 5p amplification detected by array-based comparative genomic hybridization is associated with poor prognosis of glioblastomas.
Cancer Sci. 96:676-683(2005)

PubMed=16391870; DOI=10.3892/or.15.2.463
Castigli E., Sciaccaluga M., Schiavoni G., Brozzi F., Fabiani R., Gorello P., Gianfranceschi G.L.
GL15 and U251 glioblastoma-derived human cell lines are peculiarly susceptible to induction of mitotic death by very low concentrations of okadaic acid.
Oncol. Rep. 15:463-470(2006)

PubMed=17088437; DOI=10.1158/1535-7163.MCT-06-0433
Ikediobi O.N., Davies H., Bignell G.R., Edkins S., Stevens C., O'Meara S., Santarius T., Avis T., Barthorpe S., Brackenbury L., Buck G., Butler A., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Hunter C., Jenkinson A., Jones D., Kosmidou V., Lugg R., Menzies A., Mironenko T., Parker A., Perry J., Raine K., Richardson D., Shepherd R., Small A., Smith R., Solomon H., Stephens P., Teague J.W., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Reinhold W.C., Weinstein J.N., Stratton M.R., Futreal P.A., Wooster R.
Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.
Mol. Cancer Ther. 5:2606-2612(2006)

PubMed=19372543; DOI=10.1158/1535-7163.MCT-08-0921
Lorenzi P.L., Reinhold W.C., Varma S., Hutchinson A.A., Pommier Y., Chanock S.J., Weinstein J.N.
DNA fingerprinting of the NCI-60 cell line panel.
Mol. Cancer Ther. 8:713-724(2009)

PubMed=19435942; DOI=10.1215/15228517-2009-025
Ichimura K., Pearson D.M., Kocialkowski S., Backlund L.M., Chan R., Jones D.T.W., Collins V.P.
IDH1 mutations are present in the majority of common adult gliomas but rare in primary glioblastomas.
Neuro-oncol. 11:341-347(2009)

PubMed=22336246; DOI=10.1016/j.bmc.2012.01.017
Kong D., Yamori T.
JFCR39, a panel of 39 human cancer cell lines, and its application in the discovery and development of anticancer drugs.
Bioorg. Med. Chem. 20:1947-1951(2012)

PubMed=22384151; DOI=10.1371/journal.pone.0032096
Lee J.-S., Kim Y.K., Kim H.J., Hajar S., Tan Y.L., Kang N.-Y., Ng S.H., Yoon C.N., Chang Y.-T.
Identification of cancer cell-line origins using fluorescence image-based phenomic screening.
PLoS ONE 7:E32096-E32096(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22570425; DOI=10.1093/neuonc/nos072
Bady P., Diserens A.-C., Castella V., Kalt S., Heinimann K., Hamou M.-F., Delorenzi M., Hegi M.E.
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Neuro-oncol. 14:701-711(2012)

PubMed=22628656; DOI=10.1126/science.1218595
Jain M., Nilsson R., Sharma S., Madhusudhan N., Kitami T., Souza A.L., Kafri R., Kirschner M.W., Clish C.B., Mootha V.K.
Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation.
Science 336:1040-1044(2012)

PubMed=23856246; DOI=10.1158/0008-5472.CAN-12-3342
Abaan O.D., Polley E.C., Davis S.R., Zhu Y.J., Bilke S., Walker R.L., Pineda M., Gindin Y., Jiang Y., Reinhold W.C., Holbeck S.L., Simon R.M., Doroshow J.H., Pommier Y., Meltzer P.S.
The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology.
Cancer Res. 73:4372-4382(2013)

PubMed=23933261; DOI=10.1016/j.celrep.2013.07.018
Moghaddas Gholami A., Hahne H., Wu Z., Auer F.J., Meng C., Wilhelm M., Kuster B.
Global proteome analysis of the NCI-60 cell line panel.
Cell Rep. 4:609-620(2013)

PubMed=24279929; DOI=10.1186/2049-3002-1-20
Dolfi S.C., Chan L.L.-Y., Qiu J., Tedeschi P.M., Bertino J.R., Hirshfield K.M., Oltvai Z.N., Vazquez A.
The metabolic demands of cancer cells are coupled to their size and protein synthesis rates.
Cancer Metab. 1:20-20(2013)

PubMed=24670534; DOI=10.1371/journal.pone.0092047
Varma S., Pommier Y., Sunshine M., Weinstein J.N., Reinhold W.C.
High resolution copy number variation data in the NCI-60 cancer cell lines from whole genome microarrays accessible through CellMiner.
PLoS ONE 9:E92047-E92047(2014)

PubMed=24810477; DOI=10.1002/cam4.219
Torsvik A., Stieber D., Enger P.O., Golebiewska A., Molven A., Svendsen A., Westermark B., Niclou S.P., Olsen T.K., Chekenya Enger M., Bjerkvig R.
U-251 revisited: genetic drift and phenotypic consequences of long-term cultures of glioblastoma cells.
Cancer Med. 3:812-824(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

PubMed=27377824; DOI=10.1038/sdata.2016.52
Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.
Sci. Data 3:160052-160052(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27582061; DOI=10.1126/scitranslmed.aaf6853
Allen M., Bjerke M., Edlund H., Nelander S., Westermark B.
Origin of the U87MG glioma cell line: good news and bad news.
Sci. Transl. Med. 8:354re3-354re3(2016)

PubMed=27807467; DOI=10.1186/s13100-016-0078-4
Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.
A map of mobile DNA insertions in the NCI-60 human cancer cell panel.
Mob. DNA 7:20-20(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections CLS; 300385/p534_U251_MG
ECACC; 09063001
ICLC; HTL99014
IFO; IFO50288
KCB; KCB 200965YJ
NCI-DTP; U-251
RCB; RCB0461 - Discontinued
Cell line databases/resources CLDB; cl4583
CCLE; U251MG_CENTRAL_NERVOUS_SYSTEM
CCRID; 3111C0001CCC000058
CCRID; 3131C0001000700058
CCRID; 3142C0001000000112
CGH-DB; 155-1
Cosmic-CLP; 905983
GDSC; 905983
LINCS_LDP; LCL-1400
Lonza; 105
Lonza; 1007
SKY/M-FISH/CGH; 2825
TOKU-E; 4005
Ontologies BTO; BTO:0002035
CLO; CLO_0009456
EFO; EFO_0006498
Chemistry resources ChEMBL-Cells; CHEMBL3307756
ChEMBL-Targets; CHEMBL615021
ChEMBL-Targets; CHEMBL615022
Gene expression databases GEO; GSM2103
GEO; GSM50180
GEO; GSM50244
GEO; GSM750795
GEO; GSM799328
GEO; GSM799391
GEO; GSM847166
GEO; GSM844718
GEO; GSM844719
GEO; GSM887720
GEO; GSM888814
GEO; GSM1153398
GEO; GSM1181247
GEO; GSM1181342
GEO; GSM1670555
GEO; GSM2124691
Polymorphism and mutation databases Cosmic; 687451
Cosmic; 848340
Cosmic; 849850
Cosmic; 850742
Cosmic; 875896
Cosmic; 897440
Cosmic; 905983
Cosmic; 920818
Cosmic; 923899
Cosmic; 970094
Cosmic; 974243
Cosmic; 1044250
Cosmic; 1066235
Cosmic; 1092644
Cosmic; 1171225
Cosmic; 1175823
Cosmic; 1217673
Cosmic; 1312345
Cosmic; 1436038
Cosmic; 1610741
Cosmic; 1998487
Cosmic; 2367502
Cosmic; 2367554
Cosmic; 2516042
Cosmic; 2550363
Proteomic databases PRIDE; PXD000589