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Cellosaurus U-87MG ATCC (CVCL_0022)

Cell line name U-87MG ATCC
Synonyms U-87MG; U-87 MG; U87 MG; U-87-MG; U87-MG; U87MG; U-87; U87; 87 MG; 87MG
Accession CVCL_0022
Resource Identification Initiative To cite this cell line use: U-87MG ATCC (RRID:CVCL_0022)
Comments Problematic cell line: Misidentified. This cell line is not the original glioblastoma cell line established in 1968 at the University of Uppsala. As described in PubMed=27582061 it is most probably also a glioblastoma cell line but whose origin is unknow. See U-87MG Uppsala (CVCL_GP63) for the original U-87MG cell line.
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: ENCODE project common cell types; tier 3.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00535.
Doubling time: 72 hours (PubMed=25984343); ~34 hours (PBCF).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Cell surface proteome.
Omics: CNV analysis.
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Genome sequenced.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Has no IDH1 mutation (PubMed=19435942).
HLA typing Source: PubMed=25960936
Class I
HLA-AA*02:01,02:01
HLA-BB*44:02,44:02
HLA-CC*05:01,05:01
Class II
HLA-DQDQA1*01:02,01:02
DQB1*06:02,06:02
HLA-DRDRB1*15:01,13:07
Disease Glioblastoma (NCIt: C3058)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_5J15 (U-87 MG-luc2)CVCL_UE09 (U-87MG ATCC IDH1 p.R132H)CVCL_VL16 (U-87MG/DDP)
CVCL_5J12 (U87 MG-Red-FLuc)CVCL_X702 (U87-CD4 Clone 2)CVCL_4V16 (U87-MG/Pt)
CVCL_3428 (U87/DK)CVCL_3429 (U87/WT)CVCL_X626 (U87.CD4)
CVCL_JY78 (U87MG.deltaEGFR)CVCL_JY80 (U87MG.DY1)CVCL_JY81 (U87MG.DY2)
CVCL_JY82 (U87MG.DY3)CVCL_JY83 (U87MG.DY4)CVCL_JY84 (U87MG.DY5)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CLS; Cosmic-CLP; ECACC; KCLB; PubMed=11416159; PubMed=22570425; PubMed=25877200; PubMed=27582061

Markers:
AmelogeninX,Y (ATCC; Cosmic-CLP; KCLB)
X (AddexBio; CLS; ECACC; PubMed=11416159; PubMed=22570425; PubMed=25877200; PubMed=27582061)
CSF1PO10,11
D13S3178,11
D16S53912
D18S5113 (CLS; PubMed=22570425)
13,14 (PubMed=11416159; PubMed=25877200)
D19S43315,15.2
D21S1128,32.2
D2S133820,23
D3S135816,17
D5S81811,12
D7S8208,9
D8S117910,11
FGA18,24
Penta D9,14
Penta E7,14
TH019.3
TPOX8
vWA15,17
Web pages https://en.wikipedia.org/wiki/U87
https://physics.cancer.gov/docs/bioresource/brain/NCI-PBCF-HTB14_U-87_MG_SOP-508.pdf
https://strap.nci.nih.gov/celline_detail.php?sample_id=58
http://www.proteinatlas.org/learn/cellines
http://genome.ucsc.edu/ENCODE/protocols/cell/human/U-87_Myers_protocol.pdf
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/u/cell-lines-detail-314.html
http://iclac.org/wp-content/uploads/Cross-Contaminations_v9_distribution.xlsx
Publications

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=450131; DOI=10.1038/279797a0
Day R.S. III, Ziolkowski C.H.J.
Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5.
Nature 279:797-799(1979)

PubMed=22282976; DOI=10.1093/carcin/1.1.21
Day R.S. III, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Mattern M.R.
Human tumor cell strains defective in the repair of alkylation damage.
Carcinogenesis 1:21-32(1980)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=10074188
Arbour N., Cote G., Lachance C., Tardieu M., Cashman N.R., Talbot P.J.
Acute and persistent infection of human neural cell lines by human coronavirus OC43.
J. Virol. 73:3338-3350(1999)

PubMed=10416987; DOI=10.1111/j.1750-3639.1999.tb00536.x
Ishii N., Maier D., Merlo A., Tada M., Sawamura Y., Diserens A.-C., Van Meir E.G.
Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.
Brain Pathol. 9:469-479(1999)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=11966317; DOI=10.1667/0033-7587(2002)157[0516:LDRHIH]2.0.CO;2
Chandna S., Dwarakanath B.S., Khaitan D., Mathew T.L., Jain V.
Low-dose radiation hypersensitivity in human tumor cell lines: effects of cell-cell contact and nutritional deprivation.
Radiat. Res. 157:516-525(2002)

PubMed=16232199; DOI=10.1111/j.1349-7006.2005.00099.x
Saigusa K., Hashimoto N., Tsuda H., Yokoi S., Maruno M., Yoshimine T., Aoyagi M., Ohno K., Imoto I., Inazawa J.
Overexpressed Skp2 within 5p amplification detected by array-based comparative genomic hybridization is associated with poor prognosis of glioblastomas.
Cancer Sci. 96:676-683(2005)

PubMed=19365568; DOI=10.1371/journal.pone.0005209
Bax D.A., Little S.E., Gaspar N., Perryman L., Marshall L., Viana-Pereira M., Jones T.A., Williams R.D., Grigoriadis A., Vassal G., Workman P., Sheer D., Reis R.M., Pearson A.D.J., Hargrave D., Jones C.
Molecular and phenotypic characterisation of paediatric glioma cell lines as models for preclinical drug development.
PLoS ONE 4:E5209-E5209(2009)

PubMed=19435942; DOI=10.1215/15228517-2009-025
Ichimura K., Pearson D.M., Kocialkowski S., Backlund L.M., Chan R., Jones D.T.W., Collins V.P.
IDH1 mutations are present in the majority of common adult gliomas but rare in primary glioblastomas.
Neuro-oncol. 11:341-347(2009)

PubMed=20126413; DOI=10.1371/journal.pgen.1000832
Clark M.J., Homer N., O'Connor B.D., Chen Z., Eskin A., Lee H., Merriman B., Nelson S.F.
U87MG decoded: the genomic sequence of a cytogenetically aberrant human cancer cell line.
PLoS Genet. 6:E1000832-E1000832(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20587068; DOI=10.1186/1471-2407-10-339
Nakabayashi H., Yawata T., Shimizu K.
Anti-invasive and antiangiogenic effects of MMI-166 on malignant glioma cells.
BMC Cancer 10:339-339(2010)

PubMed=21406405; DOI=10.1158/0008-5472.CAN-10-3112
Grzmil M., Morin P. Jr., Lino M.M., Merlo A., Frank S., Wang Y., Moncayo G., Hemmings B.A.
MAP kinase-interacting kinase 1 regulates SMAD2-dependent TGF-beta signaling pathway in human glioblastoma.
Cancer Res. 71:2392-2402(2011)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22570425; DOI=10.1093/neuonc/nos072
Bady P., Diserens A.-C., Castella V., Kalt S., Heinimann K., Hamou M.-F., Delorenzi M., Hegi M.E.
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Neuro-oncol. 14:701-711(2012)

PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=25960936; DOI=10.4161/21624011.2014.954893
Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:E954893-E954893(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

PubMed=26496030; DOI=10.18632/oncotarget.6171
Patil V., Pal J., Somasundaram K.
Elucidating the cancer-specific genetic alteration spectrum of glioblastoma derived cell lines from whole exome and RNA sequencing.
Oncotarget 6:43452-43471(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27582061; DOI=10.1126/scitranslmed.aaf6853
Allen M., Bjerke M., Edlund H., Nelander S., Westermark B.
Origin of the U87MG glioma cell line: good news and bad news.
Sci. Transl. Med. 8:354re3-354re3(2016)

Cross-references
Cell line collections AddexBio; C0005002/71
ATCC; HTB-14
BCRC; 60360
BCRJ; 0241
CLS; 300367/p658_U-87_MG
ECACC; 89081402
ICLC; HTL00013
IZSLER; BS TCL 189
KCB; KCB 2011101YJ
KCLB; 30014
NCBI_Iran; C531
NIH-ARP; 2188-324
RCB; RCB0419 - Discontinued
Cell line databases/resources CLDB; cl4592
CLDB; cl4594
CLDB; cl4595
CLDB; cl7166
CCLE; U87MG_CENTRAL_NERVOUS_SYSTEM
CCRID; 3111C0001CCC000208
CCRID; 3131C0001000700138
CGH-DB; 157-1
Cosmic-CLP; 687590
GDSC; 687590
LINCS_HMS; 50868
LINCS_LDP; LCL-1364
Lonza; 37
Lonza; 808
TOKU-E; 3381
Ontologies BTO; BTO:0002036
CLO; CLO_0009463
CLO; CLO_0009464
EFO; EFO_0005237
MCCL; MCC:0000469
Biological sample resources BioSample; SAMN03472715
BioSample; SAMN03473126
BioSample; SAMN05292456
ENCODE; ENCBS424ENC
Chemistry resources ChEMBL-Cells; CHEMBL3307575
ChEMBL-Targets; CHEMBL614247
Gene expression databases ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM326243
GEO; GSM481417
GEO; GSM500897
GEO; GSM887723
GEO; GSM888817
GEO; GSM923437
GEO; GSM1374974
GEO; GSM1670559
Other Wikidata; Q7863603
Polymorphism and mutation databases Cosmic; 687590
Cosmic; 849853
Cosmic; 850741
Cosmic; 850822
Cosmic; 920816
Cosmic; 1036336
Cosmic; 1064099
Cosmic; 1066234
Cosmic; 1171224
Cosmic; 1175825
Cosmic; 1198263
Cosmic; 1217671
Cosmic; 1219449
Cosmic; 1237542
Cosmic; 1294951
Cosmic; 1610737
Cosmic; 1746951
Cosmic; 1945859
Cosmic; 2302316
Cosmic; 2367555
Cosmic; 2491106
Cosmic; 2516044
Cosmic; 2550364
Cosmic; 2568870
Cosmic; 2580918
Cosmic; 2701083
Proteomic databases PRIDE; PXD000589
PRIDE; PXD000661
PRIDE; PXD001565