Cellosaurus A-549 (CVCL_0023)

Cell line name A-549
Synonyms A 549; A549; NCI-A549; A549/ATCC; A549 ATCC; A549ATCC; hA549
Accession CVCL_0023
Secondary accession CVCL_H249
Resource Identification Initiative To cite this cell line use: A-549 (RRID:CVCL_0023)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: ENCODE project common cell types; tier 2.
Part of: JFCR39 cancer cell line panel.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Part of: NCI60 cancer cell line panel.
Doubling time: 18 hours (in RPMI 1640+10% FBS), 37 hours (in ACL-3), 36 hours (in ACL-3+BSA) (PubMed=3940644); 22 hours (PubMed=25984343); 27 hours (from cell counting), 27 hours (from absorbance) (DOI=10.5897/IJBMBR2013.0154); 22.9 hours (NCI-DTP); ~40 hours (DSMZ).
Microsatellite instability: Stable (MSS) (PubMed=12661003; Sanger).
Sequence variation: Homozygous for KRAS p.Gly12Ser (PubMed=17088437).
Sequence variation: Homozygous for STK11 p.Gln37Ter (PubMed=17088437).
Omics: Acetylation analysis by proteomics.
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep membrane proteome analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Fluorescence phenotype profiling.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K9ac ChIP-seq epigenome analysis.
Omics: lncRNA expression profiling.
Omics: Metabolome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: Proteome analysis by 2D-DE/MS.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Omics: Virome analysis using proteomics.
Misspelling: A594; In PubMed=18227028.
Misspelling: A59; In PubMed=16354588.
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_JK07 (A549 EML4-ALK); CVCL_LB65 (A549 MX10); CVCL_DF39 (A549 NucLight Green); CVCL_DF40 (A549 NucLight Red); CVCL_LI35 (A549 VIM RFP); CVCL_IP03 (A549-CR); CVCL_5I73 (A549-Dual); CVCL_J242 (A549-Luc); CVCL_5J13 (A549-luc-C8); CVCL_5I86 (A549-Red-FLuc); CVCL_W218 (A549-Taxol); CVCL_IY87 (A549/8); CVCL_H268 (A549/CPT); CVCL_JY87 (A549/GFP); CVCL_KS36 (A549/NFkB-luc); CVCL_W190 (A549/TXL10); CVCL_W191 (A549/TXL20); CVCL_W192 (A549/TXL5); CVCL_4Z15 (A549.EpoB40); CVCL_4V06 (A549(VM)28); CVCL_4V07 (A549(VP)28); CVCL_U656 (AE1-2a); CVCL_8470 (AE1201); CVCL_KV97 (InCELL Hunter A549 EP300 Bromodomain); CVCL_KV98 (InCELL Hunter A549 G9a Methyltransferase); CVCL_KV99 (InCELL Hunter A549 GLP Methyltransferase); CVCL_KW00 (InCELL Hunter A549 PRMT3 Methyltransferase); CVCL_5992 (JHU-028); CVCL_W340 (K209); CVCL_5J44 (p53-RE-luc/A549); CVCL_KW16 (PathHunter A549 IkappaB Degradation)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CLS; Cosmic-CLP; DSMZ; ECACC; JCRB; KCLB; PubMed=19372543; PubMed=25877200; RCB; TKG

Markers:
AmelogeninX,Y
CSF1PO10,12
D13S31711
D16S53911,12
D18S5114,17
D19S43313
D21S1129
D2S133824
D3S135816
D5S81811
D7S8208,11
D8S117913,14
FGA23
Penta D9
Penta E7,11
TH018,9.3
TPOX8,11
vWA14
Web pages https://en.wikipedia.org/wiki/A549_cell
http://a549.com/
https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm
https://strap.nci.nih.gov/celline_detail.php?sample_id=8
http://genome.ucsc.edu/ENCODE/protocols/cell/human/A549_protocol.pdf
http://www.proteinatlas.org/learn/cellines
http://www.cells-talk.com/index.php/page/copelibrary?key=CCL-185
http://en.vircell.com/products/a-549-cell-line/
http://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/a549/a549cells.html
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/a/cell-lines-detail-58.html
http://141.61.102.20/mxdb/project/show/9191407937500
http://tcpaportal.org/mclp/
http://www.phe-culturecollections.org.uk/media/126404/a549-cell-line-profile.pdf
Publications

PubMed=4357758; DOI=10.1093/jnci/51.5.1417
Giard D.J., Aaronson S.A., Todaro G.J., Arnstein P., Kersey J.H., Dosik H., Parks W.P.
In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
J. Natl. Cancer Inst. 51:1417-1423(1973)

PubMed=175022; DOI=10.1002/ijc.2910170110
Lieber M.M., Smith B.T., Szakal A., Nelson-Rees W.A., Todaro G.J.
A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells.
Int. J. Cancer 17:62-70(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=842942; DOI=10.1164/arrd.1977.115.2.285
Smith B.T.
Cell line A549: a model system for the study of alveolar type II cell function.
Am. Rev. Respir. Dis. 115:285-293(1977)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
Wright W.C., Daniels W.P., Fogh J.
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222
Hay R.J., Williams C.D., Macy M.L., Lavappa K.S.
Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.
Am. Rev. Respir. Dis. 125:222-232(1982)

PubMed=6148444; DOI=10.1093/jnci/73.4.801
Morstyn G., Russo A., Carney D.N., Karawya E., Wilson S.H., Mitchell J.B.
Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.
J. Natl. Cancer Inst. 73:801-807(1984)

PubMed=6500159; DOI=10.1159/000163283
Gershwin M.E., Lentz D., Owens R.B.
Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.
Exp. Cell Biol. 52:361-370(1984)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3940644
Brower M., Carney D.N., Oie H.K., Gazdar A.F., Minna J.D.
Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.
Cancer Res. 46:798-806(1986)

PubMed=3945555; DOI=10.1093/nar/14.2.843
Valenzuela D.M., Groffen J.
Four human carcinoma cell lines with novel mutations in position 12 of c-K-ras oncogene.
Nucleic Acids Res. 14:843-852(1986)

PubMed=3413074; DOI=10.1073/pnas.85.16.604
Pereira-Smith O.M., Smith J.R.
Genetic analysis of indefinite division in human cells: identification of four complementation groups.
Proc. Natl. Acad. Sci. U.S.A. 85:6042-6046(1988)

PubMed=2041050; DOI=10.1093/jnci/83.11.757
Monks A., Scudiero D.A., Skehan P., Shoemaker R.H., Paull K., Vistica D.T., Hose C., Langley J., Cronise P., Vaigro-Wolff A., Gray-Goodrich M., Campbell H., Mayo J., Boyd M.
Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.
J. Natl. Cancer Inst. 83:757-766(1991)

PubMed=7736387; DOI=10.1002/1097-0142(19950515)75:10<2442::AID-CNCR2820751009>3.0.CO;2-Q
Campling B.G., Sarda I.R., Baer K.A., Pang S.C., Baker H.M., Lofters W.S., Flynn T.G.
Secretion of atrial natriuretic peptide and vasopressin by small cell lung cancer.
Cancer 75:2442-2451(1995)

PubMed=8626706; DOI=10.1074/jbc.271.19.11477
Quinn K.A., Treston A.M., Unsworth E.J., Miller M.-J., Vos M., Grimley C., Battey J., Mulshine J.L., Cuttitta F.
Insulin-like growth factor expression in human cancer cell lines.
J. Biol. Chem. 271:11477-11483(1996)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9649128; DOI=10.1038/bjc.1998.361
Yi S., Chen J.-R., Viallet J., Schwall R.H., Nakamura T., Tsao M.-S.
Paracrine effects of hepatocyte growth factor/scatter factor on non-small-cell lung carcinoma cell lines.
Br. J. Cancer 77:2162-2170(1998)

PubMed=10700174; DOI=10.1038/73432
Ross D.T., Scherf U., Eisen M.B., Perou C.M., Rees C., Spellman P., Iyer V., Jeffrey S.S., Van de Rijn M., Waltham M., Pergamenschikov A., Lee J.C.F., Lashkari D., Shalon D., Myers T.G., Weinstein J.N., Botstein D., Brown P.O.
Systematic variation in gene expression patterns in human cancer cell lines.
Nat. Genet. 24:227-235(2000)

PubMed=11005564; DOI=10.1038/sj.neo.7900094
Kohno T., Sato T., Takakura S., Takei K., Inoue K., Nishioka M., Yokota J.
Mutation and expression of the DCC gene in human lung cancer.
Neoplasia 2:300-305(2000)

PubMed=11369051; DOI=10.1016/S0165-4608(00)00363-0
Luk C., Tsao M.-S., Bayani J., Shepherd F., Squire J.A.
Molecular cytogenetic analysis of non-small cell lung carcinoma by spectral karyotyping and comparative genomic hybridization.
Cancer Genet. Cytogenet. 125:87-99(2001)

PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.-F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)

PubMed=14581340
Yokoi S., Yasui K., Iizasa T., Imoto I., Fujisawa T., Inazawa J.
TERC identified as a probable target within the 3q26 amplicon that is detected frequently in non-small cell lung cancers.
Clin. Cancer Res. 9:4705-4713(2003)

PubMed=15746151; DOI=10.1093/hmg/ddi092
Izumi H., Inoue J., Yokoi S., Hosoda H., Shibata T., Sunamori M., Hirohashi S., Inazawa J., Imoto I.
Frequent silencing of DBC1 is by genetic or epigenetic mechanisms in non-small cell lung cancers.
Hum. Mol. Genet. 14:997-1007(2005)

PubMed=17088437; DOI=10.1158/1535-7163.MCT-06-0433
Ikediobi O.N., Davies H., Bignell G.R., Edkins S., Stevens C., O'Meara S., Santarius T., Avis T., Barthorpe S., Brackenbury L., Buck G., Butler A., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Hunter C., Jenkinson A., Jones D., Kosmidou V., Lugg R., Menzies A., Mironenko T., Parker A., Perry J., Raine K., Richardson D., Shepherd R., Small A., Smith R., Solomon H., Stephens P., Teague J.W., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Reinhold W.C., Weinstein J.N., Stratton M.R., Futreal P.A., Wooster R.
Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.
Mol. Cancer Ther. 5:2606-2612(2006)

PubMed=17332333; DOI=10.1158/0008-5472.CAN-06-3339
Okabe T., Okamoto I., Tamura K., Terashima M., Yoshida T., Satoh T., Takada M., Fukuoka M., Nakagawa K.
Differential constitutive activation of the epidermal growth factor receptor in non-small cell lung cancer cells bearing EGFR gene mutation and amplification.
Cancer Res. 67:2046-2053(2007)

PubMed=19372543; DOI=10.1158/1535-7163.MCT-08-0921
Lorenzi P.L., Reinhold W.C., Varma S., Hutchinson A.A., Pommier Y., Chanock S.J., Weinstein J.N.
DNA fingerprinting of the NCI-60 cell line panel.
Mol. Cancer Ther. 8:713-724(2009)

PubMed=19472407; DOI=10.1002/humu.21028
Blanco R., Iwakawa R., Tang M., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.
A gene-alteration profile of human lung cancer cell lines.
Hum. Mutat. 30:1199-1206(2009)

PubMed=19608861; DOI=10.1126/science.1175371
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Science 325:834-840(2009)

PubMed=19657000
Rubporn A., Srisomsap C., Subhasitanont P., Chokchaichamnankit D., Chiablaem K., Svasti J., Sangvanich P.
Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line.
Cancer Genomics Proteomics 6:229-237(2009)

PubMed=22278370; DOI=10.1074/mcp.M111.014050
Geiger T., Wehner A., Schaab C., Cox J., Mann M.
Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins.
Mol. Cell. Proteomics 11:M111.014050-M111.014050(2012)

PubMed=22313637; DOI=10.4161/cbt.19238
Takata M., Chikumi H., Miyake N., Adachi K., Kanamori Y., Yamasaki A., Igishi T., Burioka N., Nanba E., Shimizu E.
Lack of AKT activation in lung cancer cells with EGFR mutation is a novel marker of cetuximab sensitivity.
Cancer Biol. Ther. 13:369-378(2012)

PubMed=22336246; DOI=10.1016/j.bmc.2012.01.017
Kong D., Yamori T.
JFCR39, a panel of 39 human cancer cell lines, and its application in the discovery and development of anticancer drugs.
Bioorg. Med. Chem. 20:1947-1951(2012)

PubMed=22384151; DOI=10.1371/journal.pone.0032096
Lee J.-S., Kim Y.K., Kim H.J., Hajar S., Tan Y.L., Kang N.-Y., Ng S.H., Yoon C.N., Chang Y.-T.
Identification of cancer cell-line origins using fluorescence image-based phenomic screening.
PLoS ONE 7:E32096-E32096(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22576795; DOI=10.3892/or.2012.1763
Ashinuma H., Takiguchi Y., Kitazono S., Kitazono-Saitoh M., Kitamura A., Chiba T., Tada Y., Kurosu K., Sakaida E., Sekine I., Tanabe N., Iwama A., Yokosuka O., Tatsumi K.
Antiproliferative action of metformin in human lung cancer cell lines.
Oncol. Rep. 28:8-14(2012)

PubMed=22628656; DOI=10.1126/science.1218595
Jain M., Nilsson R., Sharma S., Madhusudhan N., Kitami T., Souza A.L., Kafri R., Kirschner M.W., Clish C.B., Mootha V.K.
Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation.
Science 336:1040-1044(2012)

DOI=10.5897/IJBMBR2013.0154
Iloki Assanga S.B., Gil-Salido A.A., Lewis Lujan L.M., Rosas-Durazo A., Acosta-Silva A.L., Rivera-Castaneda E.G., Rubio-Pino J.L.
Cell growth curves for different cell lines and their relationship with biological activities.
Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=23404217; DOI=10.1007/s11626-013-9581-9
Chang D., Xu H.-W., Guo Y.-H., Jiang X., Liu Y., Li K.-L., Pan C.-X., Yuan M., Wang J.-F., Li T.-Z., Liu C.-T.
Simulated microgravity alters the metastatic potential of a human lung adenocarcinoma cell line.
In Vitro Cell. Dev. Biol. Anim. 49:170-177(2013)

PubMed=23856246; DOI=10.1158/0008-5472.CAN-12-3342
Abaan O.D., Polley E.C., Davis S.R., Zhu Y.J., Bilke S., Walker R.L., Pineda M., Gindin Y., Jiang Y., Reinhold W.C., Holbeck S.L., Simon R.M., Doroshow J.H., Pommier Y., Meltzer P.S.
The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology.
Cancer Res. 73:4372-4382(2013)

PubMed=23933261; DOI=10.1016/j.celrep.2013.07.018
Moghaddas Gholami A., Hahne H., Wu Z., Auer F.J., Meng C., Wilhelm M., Kuster B.
Global proteome analysis of the NCI-60 cell line panel.
Cell Rep. 4:609-620(2013)

PubMed=24135919; DOI=10.1038/ncomms3617
Balbin O.A., Prensner J.R., Sahu A., Yocum A., Shankar S., Malik R., Fermin D., Dhanasekaran S.M., Chandler B., Thomas D., Beer D.G., Cao X., Nesvizhskii A.I., Chinnaiyan A.M.
Reconstructing targetable pathways in lung cancer by integrating diverse omics data.
Nat. Commun. 4:2617-2617(2013)

PubMed=24279929; DOI=10.1186/2049-3002-1-20
Dolfi S.C., Chan L.L.-Y., Qiu J., Tedeschi P.M., Bertino J.R., Hirshfield K.M., Oltvai Z.N., Vazquez A.
The metabolic demands of cancer cells are coupled to their size and protein synthesis rates.
Cancer Metab. 1:20-20(2013)

PubMed=24618588; DOI=10.1371/journal.pone.0091433
Chernobrovkin A.L., Zubarev R.A.
Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
PLoS ONE 9:E91433-E91433(2014)

PubMed=24670534; DOI=10.1371/journal.pone.0092047
Varma S., Pommier Y., Sunshine M., Weinstein J.N., Reinhold W.C.
High resolution copy number variation data in the NCI-60 cancer cell lines from whole genome microarrays accessible through CellMiner.
PLoS ONE 9:E92047-E92047(2014)

PubMed=25120651; DOI=10.3892/ol.2014.2234
Suzuki M., Ikeda K., Shiraishi K., Eguchi A., Mori T., Yoshimoto K., Shibata H., Ito T., Baba Y., Baba H.
Aberrant methylation and silencing of IRF8 expression in non-small cell lung cancer.
Oncol. Lett. 8:1025-1030(2014)

PubMed=25475639; DOI=10.5731/pdajpst.2014.01027
Shabram P., Kolman J.L.
Evaluation of A549 as a new vaccine cell substrate: digging deeper with massively parallel sequencing.
PDA J. Pharm. Sci. Technol. 68:639-650(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26202522; DOI=10.1021/acs.jproteome.5b00477
Kitata R.B., Dimayacyac-Esleta B.R., Choong W.-K., Tsai C.-F., Lin T.-D., Tsou C.-C., Weng S.-H., Chen Y.-J., Yang P.-C., Arco S.D., Nesvizhskii A.I., Sung T.-Y., Chen Y.-J.
Mining missing membrane proteins by high-pH reverse-phase stagetip fractionation and multiple reaction monitoring mass spectrometry.
J. Proteome Res. 14:3658-3669(2015)

PubMed=26554430; DOI=10.1021/acs.analchem.5b03639
Dimayacyac-Esleta B.R., Tsai C.-F., Kitata R.B., Lin P.-Y., Choong W.-K., Lin T.-D., Wang Y.-T., Weng S.-H., Yang P.-C., Arco S.D., Sung T.-Y., Chen Y.-J.
Rapid high-pH reverse phase stagetip for sensitive small-scale membrane proteomic profiling.
Anal. Chem. 87:12016-12023(2015)

PubMed=27377824; DOI=10.1038/sdata.2016.52
Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.
Sci. Data 3:160052-160052(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27807467; DOI=10.1186/s13100-016-0078-4
Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.
A map of mobile DNA insertions in the NCI-60 human cancer cell panel.
Mob. DNA 7:20-20(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections AddexBio; C0016002/23
ATCC; CCL-185
ATCC; CRM-CCL-185
ATCC; CRL-7909 - Discontinued
BCRC; 60074
BCRJ; 0033
CLS; 300114/p686_A-549
DSMZ; ACC-107
ECACC; 86012804
ICLC; HTL03001
IFO; IFO50153
IZSLER; BS TCL 101
JCRB; JCRB0076
KCB; KCB 200434YJ
KCLB; 10185
NCBI_Iran; C137
NCI-DTP; A549
RCB; RCB0098
RCB; RCB3677
TKG; TKG 0184
Cell line databases/resources CLDB; cl202
CLDB; cl203
CLDB; cl204
CLDB; cl205
CLDB; cl206
CLDB; cl207
CLDB; cl5100
CLDB; cl7131
CCLE; A549_LUNG
CCRID; 3111C0001CCC000002
CCRID; 3111C0002000000008
CCRID; 3131C0001000700005
CCRID; 3131C0001000700150
CCRID; 3142C0001000000062
CCRID; 3153C0001000000062
CGH-DB; 202-1
CGH-DB; 9164-4
Cosmic-CLP; 905949
GDSC; 905949
IGRhCellID; A549GEO
LINCS_HMS; 50084
LINCS_LDP; LCL-1601
Lonza; 675
SKY/M-FISH/CGH; 2809
TOKU-E; 3615
Ontologies BTO; BTO:0000018
CLO; CLO_0001601
CLO; CLO_0050025
EFO; EFO_0001086
Biological sample resources BioSample; SAMN01821615
BioSample; SAMN03472926
ENCODE; ENCBS004IAZ
ENCODE; ENCBS004LAJ
ENCODE; ENCBS004RKE
ENCODE; ENCBS005TRZ
ENCODE; ENCBS006TYD
ENCODE; ENCBS007LES
ENCODE; ENCBS009FYJ
ENCODE; ENCBS009OIC
ENCODE; ENCBS011GKY
ENCODE; ENCBS015HRP
ENCODE; ENCBS018TOF
ENCODE; ENCBS021JVU
ENCODE; ENCBS023FRY
ENCODE; ENCBS025UWS
ENCODE; ENCBS027OSU
ENCODE; ENCBS029TTL
ENCODE; ENCBS032DUJ
ENCODE; ENCBS044YWQ
ENCODE; ENCBS059RHT
ENCODE; ENCBS061GHE
ENCODE; ENCBS066ACG
ENCODE; ENCBS069CLB
ENCODE; ENCBS070TPK
ENCODE; ENCBS072GEK
ENCODE; ENCBS076NFI
ENCODE; ENCBS083TEY
ENCODE; ENCBS086UGG
ENCODE; ENCBS087LLT
ENCODE; ENCBS090WQR
ENCODE; ENCBS091SYA
ENCODE; ENCBS092WKJ
ENCODE; ENCBS093FKM
ENCODE; ENCBS096ODB
ENCODE; ENCBS097ZMG
ENCODE; ENCBS099QUK
ENCODE; ENCBS100LYC
ENCODE; ENCBS106FRF
ENCODE; ENCBS106VMV
ENCODE; ENCBS107VVO
ENCODE; ENCBS108SIA
ENCODE; ENCBS108VDW
ENCODE; ENCBS109SYJ
ENCODE; ENCBS116MCD
ENCODE; ENCBS117OOH
ENCODE; ENCBS117POP
ENCODE; ENCBS121YTQ
ENCODE; ENCBS127GWZ
ENCODE; ENCBS131VWP
ENCODE; ENCBS133BUS
ENCODE; ENCBS133KFY
ENCODE; ENCBS139BXF
ENCODE; ENCBS142GEF
ENCODE; ENCBS144NAE
ENCODE; ENCBS146CRN
ENCODE; ENCBS148XSC
ENCODE; ENCBS151QJK
ENCODE; ENCBS153BPN
ENCODE; ENCBS154OIS
ENCODE; ENCBS157WWD
ENCODE; ENCBS157YZR
ENCODE; ENCBS159AAA
ENCODE; ENCBS160AAA
ENCODE; ENCBS161AAA
ENCODE; ENCBS162AAA
ENCODE; ENCBS163AAA
ENCODE; ENCBS163NSQ
ENCODE; ENCBS164AAA
ENCODE; ENCBS165AAA
ENCODE; ENCBS166AAA
ENCODE; ENCBS167AAA
ENCODE; ENCBS167GTR
ENCODE; ENCBS168AAA
ENCODE; ENCBS169AAA
ENCODE; ENCBS170AAA
ENCODE; ENCBS176HWE
ENCODE; ENCBS178CIW
ENCODE; ENCBS179WSX
ENCODE; ENCBS181LVW
ENCODE; ENCBS182KPQ
ENCODE; ENCBS183GJP
ENCODE; ENCBS185EJU
ENCODE; ENCBS189KLL
ENCODE; ENCBS191VYO
ENCODE; ENCBS194QRX
ENCODE; ENCBS198BBA
ENCODE; ENCBS198WYR
ENCODE; ENCBS198XRT
ENCODE; ENCBS204ZVO
ENCODE; ENCBS213WGX
ENCODE; ENCBS218RFX
ENCODE; ENCBS219THB
ENCODE; ENCBS229QHJ
ENCODE; ENCBS230KLP
ENCODE; ENCBS232SYQ
ENCODE; ENCBS235ANB
ENCODE; ENCBS236JDF
ENCODE; ENCBS239RRE
ENCODE; ENCBS241DOQ
ENCODE; ENCBS246IJP
ENCODE; ENCBS257YHA
ENCODE; ENCBS257YLQ
ENCODE; ENCBS260HPC
ENCODE; ENCBS261PDK
ENCODE; ENCBS262AII
ENCODE; ENCBS266ZMP
ENCODE; ENCBS270TGZ
ENCODE; ENCBS272CXK
ENCODE; ENCBS273XQH
ENCODE; ENCBS277AMO
ENCODE; ENCBS277NZL
ENCODE; ENCBS281AZP
ENCODE; ENCBS287FJW
ENCODE; ENCBS290TVV
ENCODE; ENCBS291TVJ
ENCODE; ENCBS293IUY
ENCODE; ENCBS299XUO
ENCODE; ENCBS302GLU
ENCODE; ENCBS306KID
ENCODE; ENCBS307KAV
ENCODE; ENCBS314UJS
ENCODE; ENCBS316RGG
ENCODE; ENCBS317YUF
ENCODE; ENCBS318NXZ
ENCODE; ENCBS323CFG
ENCODE; ENCBS327IUO
ENCODE; ENCBS328YSW
ENCODE; ENCBS330OQB
ENCODE; ENCBS331QMP
ENCODE; ENCBS336FCB
ENCODE; ENCBS342HZL
ENCODE; ENCBS343YHO
ENCODE; ENCBS349YRA
ENCODE; ENCBS369ESF
ENCODE; ENCBS370PMJ
ENCODE; ENCBS372GEL
ENCODE; ENCBS380JKY
ENCODE; ENCBS381CYW
ENCODE; ENCBS385PXA
ENCODE; ENCBS389XCA
ENCODE; ENCBS392TVW
ENCODE; ENCBS398XGZ
ENCODE; ENCBS399VKP
ENCODE; ENCBS405EQX
ENCODE; ENCBS405WSN
ENCODE; ENCBS410NHM
ENCODE; ENCBS417IIV
ENCODE; ENCBS419HCP
ENCODE; ENCBS419TTO
ENCODE; ENCBS420GJD
ENCODE; ENCBS420ZHM
ENCODE; ENCBS423AHV
ENCODE; ENCBS423ENC
ENCODE; ENCBS423SRU
ENCODE; ENCBS430EPP
ENCODE; ENCBS431FWG
ENCODE; ENCBS435PZT
ENCODE; ENCBS444ENC
ENCODE; ENCBS444MVA
ENCODE; ENCBS445ENC
ENCODE; ENCBS446ENC
ENCODE; ENCBS447ENC
ENCODE; ENCBS447LYH
ENCODE; ENCBS448ENC
ENCODE; ENCBS449ENC
ENCODE; ENCBS450ENC
ENCODE; ENCBS451ENC
ENCODE; ENCBS452TOO
ENCODE; ENCBS455PIS
ENCODE; ENCBS460QXP
ENCODE; ENCBS461GBL
ENCODE; ENCBS461XLE
ENCODE; ENCBS462FXS
ENCODE; ENCBS462SQL
ENCODE; ENCBS466BWX
ENCODE; ENCBS468TPT
ENCODE; ENCBS471LLC
ENCODE; ENCBS473OMG
ENCODE; ENCBS477BQZ
ENCODE; ENCBS478IHD
ENCODE; ENCBS478XMC
ENCODE; ENCBS479HSK
ENCODE; ENCBS485UNU
ENCODE; ENCBS487FRL
ENCODE; ENCBS488EBW
ENCODE; ENCBS488ZDO
ENCODE; ENCBS490YBT
ENCODE; ENCBS492GFZ
ENCODE; ENCBS497NSM
ENCODE; ENCBS498NRD
ENCODE; ENCBS503NHT
ENCODE; ENCBS507SWE
ENCODE; ENCBS508IUN
ENCODE; ENCBS508LJT
ENCODE; ENCBS514NHD
ENCODE; ENCBS518HSM
ENCODE; ENCBS518WHI
ENCODE; ENCBS525GZL
ENCODE; ENCBS528HKR
ENCODE; ENCBS538GZG
ENCODE; ENCBS540LZG
ENCODE; ENCBS547ACC
ENCODE; ENCBS547VCK
ENCODE; ENCBS547WFZ
ENCODE; ENCBS549TVN
ENCODE; ENCBS552KSE
ENCODE; ENCBS553BBK
ENCODE; ENCBS555HHK
ENCODE; ENCBS561DRV
ENCODE; ENCBS570ODA
ENCODE; ENCBS570PSF
ENCODE; ENCBS570WAL
ENCODE; ENCBS573ZZW
ENCODE; ENCBS574SHM
ENCODE; ENCBS581KGX
ENCODE; ENCBS582CLM
ENCODE; ENCBS583GBH
ENCODE; ENCBS593PHP
ENCODE; ENCBS594NFK
ENCODE; ENCBS597DCL
ENCODE; ENCBS598CCT
ENCODE; ENCBS598OWL
ENCODE; ENCBS599ALV
ENCODE; ENCBS600ZKV
ENCODE; ENCBS606CKR
ENCODE; ENCBS606KBB
ENCODE; ENCBS606UTY
ENCODE; ENCBS608WXT
ENCODE; ENCBS609BAO
ENCODE; ENCBS611JSC
ENCODE; ENCBS613VBS
ENCODE; ENCBS615RCL
ENCODE; ENCBS616EUG
ENCODE; ENCBS620TKD
ENCODE; ENCBS620YUM
ENCODE; ENCBS626ALW
ENCODE; ENCBS630KEQ
ENCODE; ENCBS633FJA
ENCODE; ENCBS640AFN
ENCODE; ENCBS643KEB
ENCODE; ENCBS646DYP
ENCODE; ENCBS646TBQ
ENCODE; ENCBS652QEI
ENCODE; ENCBS657XCY
ENCODE; ENCBS658RMM
ENCODE; ENCBS659JPG
ENCODE; ENCBS664ATC
ENCODE; ENCBS675TOV
ENCODE; ENCBS678LVM
ENCODE; ENCBS681JBN
ENCODE; ENCBS682PYF
ENCODE; ENCBS684TZE
ENCODE; ENCBS685EHX
ENCODE; ENCBS685QBT
ENCODE; ENCBS686TDM
ENCODE; ENCBS687HVT
ENCODE; ENCBS691ANI
ENCODE; ENCBS700HNY
ENCODE; ENCBS701ZPZ
ENCODE; ENCBS705AWL
ENCODE; ENCBS706OMV
ENCODE; ENCBS708WVC
ENCODE; ENCBS712HYP
ENCODE; ENCBS712NKI
ENCODE; ENCBS714CHO
ENCODE; ENCBS714IGN
ENCODE; ENCBS719BFR
ENCODE; ENCBS719FNK
ENCODE; ENCBS720VDH
ENCODE; ENCBS721HGM
ENCODE; ENCBS721JKR
ENCODE; ENCBS722NAW
ENCODE; ENCBS724JIH
ENCODE; ENCBS731OAC
ENCODE; ENCBS734NQI
ENCODE; ENCBS735SFW
ENCODE; ENCBS736RMD
ENCODE; ENCBS741LOB
ENCODE; ENCBS742QNT
ENCODE; ENCBS744CZP
ENCODE; ENCBS745XRX
ENCODE; ENCBS747KQQ
ENCODE; ENCBS749QGQ
ENCODE; ENCBS754LRU
ENCODE; ENCBS756YTM
ENCODE; ENCBS772RGZ
ENCODE; ENCBS774FRJ
ENCODE; ENCBS777ZVL
ENCODE; ENCBS779CAS
ENCODE; ENCBS780PIR
ENCODE; ENCBS780YEC
ENCODE; ENCBS784LUH
ENCODE; ENCBS787TLC
ENCODE; ENCBS787WXX
ENCODE; ENCBS788QQI
ENCODE; ENCBS788RGJ
ENCODE; ENCBS790LFN
ENCODE; ENCBS792THF
ENCODE; ENCBS795HVL
ENCODE; ENCBS797SEZ
ENCODE; ENCBS798DNC
ENCODE; ENCBS804CVK
ENCODE; ENCBS805BJW
ENCODE; ENCBS807RCH
ENCODE; ENCBS808EGR
ENCODE; ENCBS810BRT
ENCODE; ENCBS815HMT
ENCODE; ENCBS818CJE
ENCODE; ENCBS818RAI
ENCODE; ENCBS820MUM
ENCODE; ENCBS821RUT
ENCODE; ENCBS823HFB
ENCODE; ENCBS828JTD
ENCODE; ENCBS834ANM
ENCODE; ENCBS837CSI
ENCODE; ENCBS844DON
ENCODE; ENCBS844JPS
ENCODE; ENCBS848SUF
ENCODE; ENCBS851NMG
ENCODE; ENCBS858LUL
ENCODE; ENCBS859WOF
ENCODE; ENCBS861FXN
ENCODE; ENCBS868JZZ
ENCODE; ENCBS875AZA
ENCODE; ENCBS875EJK
ENCODE; ENCBS875EKF
ENCODE; ENCBS875WSM
ENCODE; ENCBS876BXS
ENCODE; ENCBS876CAE
ENCODE; ENCBS884PLU
ENCODE; ENCBS886DPW
ENCODE; ENCBS887WBA
ENCODE; ENCBS887WFS
ENCODE; ENCBS888YJZ
ENCODE; ENCBS889QXH
ENCODE; ENCBS890NQU
ENCODE; ENCBS892LLD
ENCODE; ENCBS894DBY
ENCODE; ENCBS896RCE
ENCODE; ENCBS897QKO
ENCODE; ENCBS898FLP
ENCODE; ENCBS901YZG
ENCODE; ENCBS914AZQ
ENCODE; ENCBS914SLE
ENCODE; ENCBS915DYB
ENCODE; ENCBS929VTV
ENCODE; ENCBS933OVE
ENCODE; ENCBS933PFW
ENCODE; ENCBS938EZW
ENCODE; ENCBS938LTP
ENCODE; ENCBS944KBZ
ENCODE; ENCBS947WTB
ENCODE; ENCBS950YDL
ENCODE; ENCBS951SRH
ENCODE; ENCBS952KGJ
ENCODE; ENCBS953AYB
ENCODE; ENCBS953UYS
ENCODE; ENCBS960LFK
ENCODE; ENCBS962DEK
ENCODE; ENCBS969VAO
ENCODE; ENCBS972JHA
ENCODE; ENCBS976TIO
ENCODE; ENCBS979STP
ENCODE; ENCBS981XNC
ENCODE; ENCBS993AJE
ENCODE; ENCBS996NTP
ENCODE; ENCBS996ZFV
ENCODE; ENCBS998UBJ
Chemistry resources ChEMBL-Cells; CHEMBL3307651
ChEMBL-Targets; CHEMBL392
Gene expression databases GEO; GSM2114
GEO; GSM50209
GEO; GSM50272
GEO; GSM185869
GEO; GSM206445
GEO; GSM253306
GEO; GSM274739
GEO; GSM274740
GEO; GSM353234
GEO; GSM434265
GEO; GSM513947
GEO; GSM514317
GEO; GSM651560
GEO; GSM651561
GEO; GSM729842
GEO; GSM729843
GEO; GSM729844
GEO; GSM729845
GEO; GSM729846
GEO; GSM729847
GEO; GSM729848
GEO; GSM729849
GEO; GSM750779
GEO; GSM750780
GEO; GSM750788
GEO; GSM750789
GEO; GSM750791
GEO; GSM784244
GEO; GSM794260
GEO; GSM799336
GEO; GSM799399
GEO; GSM816649
GEO; GSM827464
GEO; GSM846285
GEO; GSM843439
GEO; GSM886858
GEO; GSM887923
GEO; GSM923418
GEO; GSM923420
GEO; GSM923425
GEO; GSM923432
GEO; GSM923433
GEO; GSM923435
GEO; GSM945243
GEO; GSM945244
GEO; GSM1086289
GEO; GSM1086290
GEO; GSM1153406
GEO; GSM1181254
GEO; GSM1181311
GEO; GSM1181344
GEO; GSM1181353
GEO; GSM1181354
GEO; GSM1181355
GEO; GSM1181356
GEO; GSM1181364
GEO; GSM1374385
GEO; GSM1374386
GEO; GSM1374387
GEO; GSM1557133
GEO; GSM1669589
GEO; GSM2124637
Metabolomic databases MetaboLights; MTBLS102
Other Wikidata; Q4649475
Polymorphism and mutation databases Cosmic; 713864
Cosmic; 716170
Cosmic; 719675
Cosmic; 735605
Cosmic; 755468
Cosmic; 801571
Cosmic; 844592
Cosmic; 844826
Cosmic; 875854
Cosmic; 876152
Cosmic; 877443
Cosmic; 889090
Cosmic; 905949
Cosmic; 910552
Cosmic; 910560
Cosmic; 914947
Cosmic; 917386
Cosmic; 917972
Cosmic; 929148
Cosmic; 929962
Cosmic; 932915
Cosmic; 961833
Cosmic; 974287
Cosmic; 1004698
Cosmic; 1017830
Cosmic; 1028950
Cosmic; 1047097
Cosmic; 1089216
Cosmic; 1092591
Cosmic; 1146871
Cosmic; 1152518
Cosmic; 1154597
Cosmic; 1188587
Cosmic; 1219057
Cosmic; 1239867
Cosmic; 1305342
Cosmic; 1312328
Cosmic; 1352406
Cosmic; 1434961
Cosmic; 1436014
Cosmic; 1466799
Cosmic; 1571729
Cosmic; 1600605
Cosmic; 1608839
Cosmic; 1656896
Cosmic; 1802312
Cosmic; 1870260
Cosmic; 1945870
Cosmic; 1995337
Cosmic; 1998433
Cosmic; 2036687
Cosmic; 2042861
Cosmic; 2125176
Cosmic; 2301571
Cosmic; 2433750
Cosmic; 2630410
Proteomic databases PRIDE; PXD000418
PRIDE; PXD001548
PRIDE; PXD002224
PRIDE; PXD002395
PRIDE; PXD003627