Cellosaurus SiHa (CVCL_0032)

Cell line name SiHa
Synonyms Siha; SIHA
Accession CVCL_0032
Resource Identification Initiative To cite this cell line use: SiHa (RRID:CVCL_0032)
Comments Part of: COSMIC cell lines project.
Characteristics: Contains a complete HPV16 genome with a disruption of the E2 region and a small deletion.
Microsatellite instability: Stable (MSS) (Sanger).
Transformant: NCBI_TaxID; 333760; Human papillomavirus type 16 (HPV16).
Omics: CNV analysis.
Omics: Deep antibody staining analysis.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Human papillomavirus-related cervical squamous cell carcinoma (NCIt: C27676)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_X529 (SiHa-NTRpuro)CVCL_K031 (SiHa/cDDP)
Sex of cell Female
Age at sampling 55Y
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; Cosmic-CLP; KCLB; PubMed=25877200

Markers:
AmelogeninX
CSF1PO12
D13S31711
D16S53912
D18S5115
D21S1129,31
D3S135816,17
D5S8189
D7S82010
D8S117913,16
FGA21
Penta D9,12
Penta E10,12
TH016,9
TPOX8
vWA14,17
Web pages http://www.proteinatlas.org/learn/cellines
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-22.html
Publications

PubMed=5529598; DOI=10.3181/00379727-135-35091a
Friedl F., Kimura I., Osato T., Ito Y.
Studies on a new human cell line (SiHa) derived from carcinoma of uterus. I. Its establishment and morphology.
Proc. Soc. Exp. Biol. Med. 135:543-545(1970)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=2990217
Yee C., Krishnan-Hewlett I., Baker C.C., Schlegel R., Howley P.M.
Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.
Am. J. Pathol. 119:361-366(1985)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=3029430
Baker C.C., Phelps W.C., Lindgren V., Braun M.J., Gonda M.A., Howley P.M.
Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.
J. Virol. 61:962-971(1987)

PubMed=3029760; DOI=10.1073/pnas.84.4.1070
Durst M., Croce C.M., Gissmann L., Schwarz E., Huebner K.
Papillomavirus sequences integrate near cellular oncogenes in some cervical carcinomas.
Proc. Natl. Acad. Sci. U.S.A. 84:1070-1074(1987)

PubMed=1394225
Somers K.D., Merrick M.A., Lopez M.E., Incognito L.S., Schechter G.L., Casey G.
Frequent p53 mutations in head and neck cancer.
Cancer Res. 52:5997-6000(1992)

PubMed=15531914; DOI=10.1038/sj.onc.1208235
Baldus S.E., Schwarz E., Lohrey C., Zapatka M., Landsberg S., Hahn S.A., Schmidt D., Dienes H.-P., Schmiegel W.H., Schwarte-Waldhoff I.
Smad4 deficiency in cervical carcinoma cells.
Oncogene 24:810-819(2005)

PubMed=17311676; DOI=10.1186/1471-2164-8-53
Kloth J.N., Oosting J., van Wezel T., Szuhai K., Knijnenburg J., Gorter A., Kenter G.G., Fleuren G.J., Jordanova E.S.
Combined array-comparative genomic hybridization and single-nucleotide polymorphism-loss of heterozygosity analysis reveals complex genetic alterations in cervical cancer.
BMC Genomics 8:53-53(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20429727; DOI=10.3109/02841861003614343
Sorensen B.S., Toustrup K., Horsman M.R., Overgaard J., Alsner J.
Identifying pH independent hypoxia induced genes in human squamous cell carcinomas in vitro.
Acta Oncol. 49:895-905(2010)

PubMed=21439369; DOI=10.1016/j.mcp.2011.03.003
Schmitt M., Pawlita M.
The HPV transcriptome in HPV16 positive cell lines.
Mol. Cell. Probes 25:108-113(2011)

PubMed=23881600; DOI=10.1002/jcp.24433
Ravi M., Sah S., Bhammar R.
Differences of SiHa (human cancer of cervix) and BMG-1 (brain glioma) cell lines as 2D and 3D cultures.
J. Cell. Physiol. 229:127-131(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

Cross-references
Cell line collections AddexBio; C0008002/372
ATCC; HTB-35
ICLC; HTL98017
IZSLER; BS TCL 112
KCB; KCB 2013026YJ
KCLB; 30035
Cell line databases/resources CLDB; cl4298
CLDB; cl4299
CLDB; cl4900
CCRID; 3111C0001CCC000294
CCRID; 3131C0001000700113
CCRID; 3142C0001000000089
Cosmic-CLP; 930297
GDSC; 930297
IGRhCellID; SiHa
LINCS_LDP; LCL-1542
TOKU-E; 3119
Ontologies BTO; BTO:0002210
CLO; CLO_0009017
EFO; EFO_0002379
MCCL; MCC:0000451
Biological sample resources BioSample; SAMN03473215
Chemistry resources ChEMBL-Cells; CHEMBL3307968
ChEMBL-Targets; CHEMBL612542
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-3610
GEO; GSM113866
GEO; GSM226740
GEO; GSM226876
GEO; GSM253302
GEO; GSM675738
GEO; GSM685444
GEO; GSM723057
GEO; GSM723058
GEO; GSM1670422
GEO; GSM1716411
GEO; GSM1716412
GEO; GSM1716413
GEO; GSM1716414
GEO; GSM1716415
GEO; GSM1716416
GEO; GSM1716423
GEO; GSM1716424
GEO; GSM1716425
GEO; GSM1716426
Other Wikidata; Q54953470
Polymorphism and mutation databases Cosmic; 760220
Cosmic; 809229
Cosmic; 846184
Cosmic; 877467
Cosmic; 925356
Cosmic; 928911
Cosmic; 930297
Cosmic; 1019306
Cosmic; 1131517
Cosmic; 1193028
Cosmic; 1201773
Cosmic; 1324212
Cosmic; 1995627
Cosmic; 2301542
Cosmic; 2660193
Proteomic databases PRIDE; PXD001806