Cellosaurus SK-BR-3 (CVCL_0033)

Cell line name SK-BR-3
Synonyms SK-Br-3; Sk-Br-3; SKBR-3; SKBr-3; SK-BR3; SKBr3; SkBr3; SKBR3
Accession CVCL_0033
Resource Identification Initiative To cite this cell line use: SK-BR-3 (RRID:CVCL_0033)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: ICBP43 breast cancer cell line panel.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
From: Memorial Sloan-Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan-Kettering Cancer Center Office of Technology Development; SK 808.
Doubling time: ~2-3 days (DSMZ).
Microsatellite instability: Stable (MSS) (PubMed=23671654).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: Deep antibody staining analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: Exosome proteome analysis.
Omics: miRNA expression profiling.
Omics: N-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: SKBR3B; In PubMed=26378940.
Disease Breast adenocarcinoma (NCIt: C5214)
Derived from metastatic site: Pleural effusion.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_4V52 (SK-BR-3-LR); CVCL_4Y11 (SK-BR-3-Luc); CVCL_EI04 (SKBR3-AZDRa); CVCL_EI05 (SKBR3-AZDRb); CVCL_EI06 (SKBR3-AZDRc); CVCL_4V68 (SKBR3-LAP[r]); CVCL_EI07 (SKBR3-LAPRa); CVCL_EI08 (SKBR3-LAPRb); CVCL_LG17 (SKBR3-LR); CVCL_LG18 (SKBR3-NR); CVCL_EQ69 (SKBR3-PR); CVCL_LG19 (SKBR3-TR)
Originate from same individual CVCL_1074 ! AU565
Sex of cell Female
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CLS; DSMZ; KCLB; PubMed=25877200; RCB

Markers:
AmelogeninX
CSF1PO12
D13S31711,12
D16S5399
D18S5110,13
D21S1130,30.2
D3S135817
D5S8189,12
D7S8209,12
D8S117911,12 (CLS)
12 (PubMed=25877200)
FGA20
Penta D9,13 (CLS)
9,12 (PubMed=25877200)
Penta E10,11
TH018,9
TPOX8,11
vWA17
Web pages https://en.wikipedia.org/wiki/SKBR3
http://www.pawefish.path.cam.ac.uk/BreastCellLineDescriptions/sk-br-3.htm
http://colt.ccbr.utoronto.ca/cancer/get_cellline.pl?cellline=SK-BR-3
http://www.proteinatlas.org/learn/cellines
http://lincs.hms.harvard.edu/resources/reagents/icbp43/
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-57.html
https://www.mskcc.org/research-advantage/support/technology/tangible-material/human-breast-cell-line-sk-br-3
http://tcpaportal.org/mclp/
Publications

DOI=10.1007/978-1-4757-1647-4_5
Fogh J., Trempe G.L.
New human tumor cell lines.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.115-159; Springer; New York (1975)

PubMed=1013510; DOI=10.1007/978-3-642-81043-5_5
Trempe G.L.
Human breast cancer in culture.
Recent Results Cancer Res. 57:33-41(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
Wright W.C., Daniels W.P., Fogh J.
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=6500159; DOI=10.1159/000163283
Gershwin M.E., Lentz D., Owens R.B.
Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.
Exp. Cell Biol. 52:361-370(1984)

PubMed=6582512; DOI=10.1073/pnas.81.2.568
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=9671407; DOI=10.1038/sj.onc.1201814
Sweeney K.J., Swarbrick A., Sutherland R.L., Musgrove E.A.
Lack of relationship between CDK activity and G1 cyclin expression in breast cancer cells.
Oncogene 16:2865-2878(1998)

PubMed=10862037; DOI=10.1002/1098-2264(200007)28:3<308::AID-GCC9>3.0.CO;2-B
Kytola S., Rummukainen J., Nordgren A., Karhu R., Farnebo F., Isola J., Larsson C.
Chromosomal alterations in 15 breast cancer cell lines by comparative genomic hybridization and spectral karyotyping.
Genes Chromosomes Cancer 28:308-317(2000)

PubMed=11044355; DOI=10.1054/bjoc.2000.1458
Davidson J.M., Gorringe K.L., Chin S.-F., Orsetti B., Besret C., Courtay-Cahen C., Roberts I., Theillet C., Caldas C., Edwards P.A.W.
Molecular cytogenetic analysis of breast cancer cell lines.
Br. J. Cancer 83:1309-1317(2000)

PubMed=12800145; DOI=10.1002/gcc.10218
Adelaide J., Huang H.-E., Murati A., Alsop A.E., Orsetti B., Mozziconacci M.-J., Popovici C., Ginestier C., Letessier A., Basset C., Courtay-Cahen C., Jacquemier J., Theillet C., Birnbaum D., Edwards P.A.W., Chaffanet M.
A recurrent chromosome translocation breakpoint in breast and pancreatic cancer cell lines targets the neuregulin/NRG1 gene.
Genes Chromosomes Cancer 37:333-345(2003)

PubMed=15677628; DOI=10.1093/carcin/bgi032
Gorringe K.L., Chin S.-F., Pharoah P., Staines J.M., Oliveira C., Edwards P.A.W., Caldas C.
Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.
Carcinogenesis 26:923-930(2005)

PubMed=16397213; DOI=10.1158/0008-5472.CAN-05-2853
Elstrodt F., Hollestelle A., Nagel J.H.A., Gorin M., Wasielewski M., van den Ouweland A., Merajver S.D., Ethier S.P., Schutte M.
BRCA1 mutation analysis of 41 human breast cancer cell lines reveals three new deleterious mutants.
Cancer Res. 66:41-45(2006)

PubMed=17157791; DOI=10.1016/j.ccr.2006.10.008
Neve R.M., Chin K., Fridlyand J., Yeh J., Baehner F.L., Fevr T., Clark L., Bayani N., Coppe J.P., Tong F., Speed T., Spellman P.T., DeVries S., Lapuk A., Wang N.J., Kuo W.L., Stilwell J.L., Pinkel D., Albertson D.G., Waldman F.M., McCormick F., Dickson R.B., Johnson M.D., Lippman M., Ethier S.P., Gazdar A.F., Gray J.W.
A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes.
Cancer Cell 10:515-527(2006)

PubMed=19582160; DOI=10.1371/journal.pone.0006146
Kao J., Salari K., Bocanegra M., Choi Y.-L., Girard L., Gandhi J., Kwei K.A., Hernandez-Boussard T., Wang P., Gazdar A.F., Minna J.D., Pollack J.R.
Molecular profiling of breast cancer cell lines defines relevant tumor models and provides a resource for cancer gene discovery.
PLoS ONE 4:E6146-E6146(2009)

PubMed=19727395; DOI=10.1371/journal.pone.0006888
Wadlow R.C., Wittner B.S., Finley S.A., Bergquist H., Upadhyay R., Finn S., Loda M., Mahmood U., Ramaswamy S.
Systems-level modeling of cancer-fibroblast interaction.
PLoS ONE 4:E6888-E6888(2009)

PubMed=20070913; DOI=10.1186/1471-2407-10-15
Tsuji K., Kawauchi S., Saito S., Furuya T., Ikemoto K., Nakao M., Yamamoto S., Oka M., Hirano T., Sasaki K.
Breast cancer cell lines carry cell line-specific genomic alterations that are distinct from aberrations in breast cancer tissues: comparison of the CGH profiles between cancer cell lines and primary cancer tissues.
BMC Cancer 10:15-15(2010)

PubMed=21247443; DOI=10.1186/gb-2011-12-1-r6
Edgren H., Murumagi A., Kangaspeska S., Nicorici D., Hongisto V., Kleivi K., Rye I.H., Nyberg S., Wolf M., Borresen-Dale A.-L., Kallioniemi O.
Identification of fusion genes in breast cancer by paired-end RNA-sequencing.
Genome Biol. 12:R6.1-R6.13(2011)

PubMed=21378333
Ford C.H.J., Al-Bader M., Al-Ayadhi B., Francis I.
Reassessment of estrogen receptor expression in human breast cancer cell lines.
Anticancer Res. 31:521-527(2011)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23151021; DOI=10.1186/1471-2164-13-619
Grigoriadis A., Mackay A., Noel E., Wu P.J., Natrajan R., Frankum J., Reis-Filho J.S., Tutt A.
Molecular characterisation of cell line models for triple-negative breast cancers.
BMC Genomics 13:619-619(2012)

PubMed=23601657; DOI=10.1186/bcr3415
Riaz M., van Jaarsveld M.T.M., Hollestelle A., Prager-van der Smissen W.J.C., Heine A.A.J., Boersma A.W.M., Liu J., Helmijr J.C.A., Ozturk B., Smid M., Wiemer E.A., Foekens J.A., Martens J.W.M.
miRNA expression profiling of 51 human breast cancer cell lines reveals subtype and driver mutation-specific miRNAs.
Breast Cancer Res. 15:R33-R33(2013)

PubMed=23671654; DOI=10.1371/journal.pone.0063056
Lu Y., Soong T.D., Elemento O.
A novel approach for characterizing microsatellite instability in cancer cells.
PLoS ONE 8:E63056-E63056(2013)

PubMed=24009699; DOI=10.1371/journal.pone.0072704
Liu X., Nie H., Zhang Y.-B., Yao Y.-F., Maitikabili A., Qu Y.-P., Shi S.-L., Chen C.-Y., Li Y.
Cell surface-specific N-glycan profiling in breast cancer.
PLoS ONE 8:E72704-E72704(2013)

PubMed=24162158; DOI=10.1007/s10549-013-2743-3
Prat A., Karginova O., Parker J.S., Fan C., He X., Bixby L., Harrell J.C., Roman E., Adamo B., Troester M., Perou C.M.
Characterization of cell lines derived from breast cancers and normal mammary tissues for the study of the intrinsic molecular subtypes.
Breast Cancer Res. Treat. 142:237-255(2013)

PubMed=24176112; DOI=10.1186/gb-2013-14-10-r110
Daemen A., Griffith O.L., Heiser L.M., Wang N.J., Enache O.M., Sanborn Z., Pepin F., Durinck S., Korkola J.E., Griffith M., Hur J.S., Huh N., Chung J., Cope L., Fackler M.J., Umbricht C., Sukumar S., Seth P., Sukhatme V.P., Jakkula L.R., Lu Y., Mills G.B., Cho R.J., Collisson E.A., van't Veer L.J., Spellman P.T., Gray J.W.
Modeling precision treatment of breast cancer.
Genome Biol. 14:R110.1-R110.14(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26055192; DOI=10.1021/acs.jproteome.5b00375
Cifani P., Kirik U., Waldemarson S., James P.
Molecular portrait of breast-cancer-derived cell lines reveals poor similarity with tumors.
J. Proteome Res. 14:2819-2827(2015)

PubMed=26378940; DOI=10.1021/acs.analchem.5b02586
Clark D.J., Fondrie W.E., Liao Z., Hanson P.I., Fulton A., Mao L., Yang A.J.
Redefining the breast cancer exosome proteome by tandem mass tag quantitative proteomics and multivariate cluster analysis.
Anal. Chem. 87:10462-10469(2015)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections AddexBio; C0006007/65
ATCC; HTB-30
BCRJ; 0226
CLS; 300333/p3803_SK-BR-3
DSMZ; ACC-736
ICLC; HTL03005
IZSLER; BS TCL 156
KCB; KCB 200723YJ
KCLB; 30030
NCBI_Iran; C207
RCB; RCB2132 - Discontinued
Cell line databases/resources CLDB; cl4310
CLDB; cl7143
CCLE; SKBR3_BREAST
CCRID; 3111C0001CCC000085
CCRID; 3131C0001000700049
IMGT/HLA; 12368
LINCS_HMS; 50057
LINCS_LDP; LCL-1475
Lonza; 70
TOKU-E; 3129
Ontologies BTO; BTO:0002419
CLO; CLO_0009034
EFO; EFO_0001236
MCCL; MCC:0000424
Biological sample resources BioSample; SAMN01821597
BioSample; SAMN03471462
Chemistry resources ChEMBL-Cells; CHEMBL3308062
ChEMBL-Targets; CHEMBL613834
Gene expression databases GEO; GSM69195
GEO; GSM73742
GEO; GSM115115
GEO; GSM149978
GEO; GSM149986
GEO; GSM149994
GEO; GSM152556
GEO; GSM152557
GEO; GSM152558
GEO; GSM152559
GEO; GSM154096
GEO; GSM154097
GEO; GSM154098
GEO; GSM154099
GEO; GSM154100
GEO; GSM154101
GEO; GSM154102
GEO; GSM154104
GEO; GSM154107
GEO; GSM155215
GEO; GSM170624
GEO; GSM170625
GEO; GSM170626
GEO; GSM170627
GEO; GSM170630
GEO; GSM170631
GEO; GSM170633
GEO; GSM170634
GEO; GSM183440
GEO; GSM183441
GEO; GSM186457
GEO; GSM186458
GEO; GSM217596
GEO; GSM274644
GEO; GSM274663
GEO; GSM344357
GEO; GSM344407
GEO; GSM350501
GEO; GSM378146
GEO; GSM481334
GEO; GSM590106
GEO; GSM595617
GEO; GSM783927
GEO; GSM827582
GEO; GSM845399
GEO; GSM847432
GEO; GSM847499
GEO; GSM844686
GEO; GSM844687
GEO; GSM887575
GEO; GSM888658
GEO; GSM1008913
GEO; GSM1038850
GEO; GSM1038851
GEO; GSM1038852
GEO; GSM1038853
GEO; GSM1038854
GEO; GSM1053720
GEO; GSM1172989
GEO; GSM1172895
GEO; GSM1214572
GEO; GSM1264069
GEO; GSM1264074
GEO; GSM1264111
GEO; GSM1264116
GEO; GSM1374872
GEO; GSM1374873
GEO; GSM1374874
GEO; GSM1401659
GEO; GSM1661988
GEO; GSM1833627
Other Wikidata; Q7390592
Polymorphism and mutation databases Cosmic; 687499
Cosmic; 755294
Cosmic; 759896
Cosmic; 871159
Cosmic; 894090
Cosmic; 897742
Cosmic; 904385
Cosmic; 921974
Cosmic; 923062
Cosmic; 934531
Cosmic; 944297
Cosmic; 979713
Cosmic; 991329
Cosmic; 997935
Cosmic; 1000127
Cosmic; 1010922
Cosmic; 1018478
Cosmic; 1044209
Cosmic; 1046958
Cosmic; 1129655
Cosmic; 1136357
Cosmic; 1176645
Cosmic; 1287914
Cosmic; 1289405
Cosmic; 1308997
Cosmic; 1434957
Cosmic; 1524342
Cosmic; 1571789
Cosmic; 1609462
Cosmic; 2036669
Cosmic; 2165013
Cosmic; 2301535
Cosmic; 2318379
Cosmic; 2361351
Proteomic databases PRIDE; PXD000691
PRIDE; PXD002053
PRIDE; PXD002057
PRIDE; PXD002281
PRIDE; PXD002486