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Cellosaurus HaCaT (CVCL_0038)

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Cell line name HaCaT
Synonyms HaCAT; HACAT; Hacat
Accession CVCL_0038
Resource Identification Initiative To cite this cell line use: HaCaT (RRID:CVCL_0038)
Comments Doubling time: 28 hours (PubMed=32938951).
Omics: Deep antibody staining analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: Metabolome analysis.
Omics: Transcriptome analysis.
Caution: Although HaCaT is distributed by a number of sources, the only official DKFZ-approved supplier is CLS.
Derived from sampling site: Back; skin; epidermis; keratinocyte.
Sequence variations Heterozygous for TP53 p.His179Tyr (c.535C>T) (ClinVar=VCV000127815) (PubMed=8504475).
Heterozygous for TP53 p.Arg282Trp (c.843_844CC>TT) (PubMed=8504475).
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_XK16 (HaCaT A-5)CVCL_HD65 (HaCaT BHLHE40 (-/-))CVCL_FA29 (HaCaT HSP70B'-GFP reporter)
CVCL_3868 (HaCaT II-4)CVCL_S024 (HaCaT SimpleCell O-GalNAc)CVCL_QX09 (HaCaT siYAP)
CVCL_QX10 (HaCaT YAP-5SA)CVCL_ZW69 (HaCaT-shNeg)CVCL_ZW70 (HaCaT-shNrf2#1)
CVCL_ZW71 (HaCaT-shNrf2#2)CVCL_ZW72 (HaCaT-shNrf2#3)CVCL_RL61 (HaCaT-TRS4)
CVCL_4Z60 (HaCaT/SM)CVCL_XK18 (HaCaT40degree)CVCL_BW57 (NOK-SI)
CVCL_A9W9 (nr-HaCat)
Sex of cell Male
Age at sampling 62Y
Category Spontaneously immortalized cell line
STR profile Source(s): CCRID; CLS; DSMZ; PubMed=11416159; PubMed=21868764

D21S1128,30.2 (CLS; PubMed=11416159)
30.2 (CCRID)
Penta D11,13
Penta E7,12

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/HaCaT

PubMed=2450098; DOI=10.1083/jcb.106.3.761
Boukamp P., Dzarlieva-Petrussevska R.T., Breitkreutz D., Hornung J., Markham A., Fusenig N.E.
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
J. Cell Biol. 106:761-771(1988)

PubMed=8162337; DOI=10.1111/j.1600-0625.1993.tb00030.x
Schurer N., Kohne A., Schliep V., Barlag K., Goerz G.
Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes.
Exp. Dermatol. 2:179-185(1993)

PubMed=8504475; DOI=10.1093/carcin/14.5.833
Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.
p53 mutations in human immortalized epithelial cell lines.
Carcinogenesis 14:833-839(1993)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9406811; DOI=10.1111/1523-1747.ep12340708
Henseleit U., Zhang J., Wanner R., Haase I., Kolde G., Rosenbach T.
Role of p53 in UVB-induced apoptosis in human HaCaT keratinocytes.
J. Invest. Dermatol. 109:722-727(1997)

PubMed=10523843; DOI=10.1038/sj.onc.1202934
Boukamp P., Popp S., Bleuel K., Tomakidi E., Burkle A., Fusenig N.E.
Tumorigenic conversion of immortal human skin keratinocytes (HaCaT) by elevated temperature.
Oncogene 18:5638-5645(1999)

PubMed=11583982; DOI=10.1016/S0002-9440(10)62541-2
Mueller M.M., Peter W., Mappes M., Huelsen A., Steinbauer H., Boukamp P., Vaccariello M., Garlick J., Fusenig N.E.
Tumor progression of skin carcinoma cells in vivo promoted by clonal selection, mutagenesis, and autocrine growth regulation by granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor.
Am. J. Pathol. 159:1567-1579(2001)

PubMed=15389883; DOI=10.1002/jcb.20212
Lemaitre G., Lamartine J., Pitaval A., Vaigot P., Garin J., Bouet S., Petat C., Soularue P., Gidrol X., Martin M.T., Waksman G.
Expression profiling of genes and proteins in HaCaT keratinocytes: proliferating versus differentiated state.
J. Cell. Biochem. 93:1048-1062(2004)

PubMed=20097197; DOI=10.1016/j.yexcr.2010.01.020
Rasanen K., Vaheri A.
Proliferation and motility of HaCaT keratinocyte derivatives is enhanced by fibroblast nemosis.
Exp. Cell Res. 316:1739-1747(2010)

PubMed=21269460; DOI=10.1186/1752-0509-5-17
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.
Initial characterization of the human central proteome.
BMC Syst. Biol. 5:17-17(2011)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

PubMed=21185708; DOI=10.1016/j.jnutbio.2010.08.004
Serini S., Donato V., Piccioni E., Trombino S., Monego G., Toesca A., Innocenti I., Missori M., De Spirito M., Celleno L., Fasano E., Ranelletti F.O., Calviello G.
Docosahexaenoic acid reverts resistance to UV-induced apoptosis in human keratinocytes: involvement of COX-2 and HuR.
J. Nutr. Biochem. 22:874-885(2016)

PubMed=26989177; DOI=10.1242/dev.133728
Elbediwy A., Vincent-Mistiaen Z.I., Spencer-Dene B., Stone R.K., Boeing S., Wculek S.K., Cordero J., Tan E.H., Ridgway R., Brunton V.G., Sahai E., Gerhardt H., Behrens A., Malanchi I., Sansom O.J., Thompson B.J.
Integrin signalling regulates YAP and TAZ to control skin homeostasis.
Development 143:1674-1687(2016)

PubMed=29660373; DOI=10.1016/j.bbagen.2018.04.012
Touat-Hamici Z., Bulteau A.-L., Bianga J., Jean-Jacques H., Szpunar J., Lobinski R., Chavatte L.
Selenium-regulated hierarchy of human selenoproteome in cancerous and immortalized cells lines.
Biochim. Biophys. Acta 1862:2493-2505(2018)

PubMed=31629070; DOI=10.1016/j.tiv.2019.104666
da Silva A.C.G., Chialchia A.R., de Castro E.G., e Silva M.R.L., Arantes D.A.C., Batista A.C., Kitten G.T., Valadares M.C.
A new corneal epithelial biomimetic 3D model for in vitro eye toxicity assessment: development, characterization and applicability.
Toxicol. In Vitro 62:104666-104666(2019)

PubMed=32938951; DOI=10.1038/s41598-020-71315-0
Pavez Lorie E., Stricker N., Plitta-Michalak B., Chen I.-P., Volkmer B., Greinert R., Jauch A., Boukamp P., Rapp A.
Characterisation of the novel spontaneously immortalized and invasively growing human skin keratinocyte line HaSKpw.
Sci. Rep. 10:15196-15196(2020)

Cell line collections AddexBio; T0020001/117
BCRJ; 0341 - Discontinued
CLS; 300493/p800_HaCaT
DSMZ; ACC-771 - Discontinued
ICLC; HL14001 - Discontinued
KCB; KCB 200442YJ
Cell line databases/resources CLDB; cl7229
CCRID; 3111C0001CCC000373
CCRID; 3142C0001000001712
Lonza; 56
TOKU-E; 3890
Ontologies BTO; BTO:0000552
EFO; EFO_0002056
Chemistry resources ChEMBL-Cells; CHEMBL3308509
ChEMBL-Targets; CHEMBL614392
Gene expression databases GEO; GSE57080
GEO; GSM913344
GEO; GSM913345
GEO; GSM960278
GEO; GSM960279
GEO; GSM960280
GEO; GSM960286
GEO; GSM960287
GEO; GSM960288
GEO; GSM960294
GEO; GSM960295
GEO; GSM960296
GEO; GSM1375594
GEO; GSM1375595
GEO; GSM1375596
GEO; GSM1375597
GEO; GSM1375598
GEO; GSM1375599
GEO; GSM1375600
GEO; GSM1375601
GEO; GSM1375602
GEO; GSM1375603
GEO; GSM1375604
GEO; GSM1375605
GEO; GSM1861816
GEO; GSM1861817
GEO; GSM1861820
GEO; GSM1861821
GEO; GSM2112559
GEO; GSM2112563
GEO; GSM2495796
GEO; GSM2495797
GEO; GSM2495798
Metabolomic databases MetaboLights; MTBLS127
Other Wikidata; Q5636366
Polymorphism and mutation databases Cosmic; 1530737
IARC_TP53; 1820
Proteomic databases PRIDE; PXD000496
PRIDE; PXD000670
PRIDE; PXD002460
PRIDE; PXD007789
PRIDE; PXD013113
Entry history
Entry creation04-Apr-2012
Last entry update12-Jan-2021
Version number31