Cellosaurus HaCaT (CVCL_0038)

Cell line name HaCaT
Synonyms HaCAT; HACAT; Hacat
Accession CVCL_0038
Resource Identification Initiative To cite this cell line use: HaCaT (RRID:CVCL_0038)
Comments Omics: Deep antibody staining analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: Transcriptome analysis.
Caution: Although HaCaT is distributed by a number of sources, the only official DKFZ-approved supplier is CLS.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_HD65 (HaCaT BHLHE40 (-/-)); CVCL_FA29 (HaCaT HSP70B'-GFP reporter); CVCL_3868 (HaCaT II-4); CVCL_QX09 (HaCat siYAP); CVCL_S024 (HaCaT SimpleCell O-GalNAc); CVCL_QX10 (HaCat YAP-5SA); CVCL_4Z60 (HaCaT/SM)
Sex of cell Male
Category Spontaneously immortalized cell line
STR profile Source(s): CLS; DSMZ; PubMed=11416159; PubMed=21868764

Markers:
AmelogeninX
CSF1PO9,11
D13S31710,12
D16S5399,12
D18S5112
D21S1128,30.2
D3S135816
D5S81812
D7S8209,11
D8S117914
FGA24
Penta D11,13
Penta E7,12
TH019.3
TPOX11,12
vWA16,17
Web pages https://en.wikipedia.org/wiki/HaCaT
http://www.proteinatlas.org/learn/cellines
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-347.html
Publications

PubMed=2450098; DOI=10.1083/jcb.106.3.761
Boukamp P., Petrussevska R.T., Breitkreutz D., Hornung J., Markham A., Fusenig N.E.
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
J. Cell Biol. 106:761-771(1988)

PubMed=8162337; DOI=10.1111/j.1600-0625.1993.tb00030.x
Schurer N., Kohne A., Schliep V., Barlag K., Goerz G.
Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes.
Exp. Dermatol. 2:179-185(1993)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=15389883; DOI=10.1002/jcb.20212
Lemaitre G., Lamartine J., Pitaval A., Vaigot P., Garin J., Bouet S., Petat C., Soularue P., Gidrol X., Martin M.T., Waksman G.
Expression profiling of genes and proteins in HaCaT keratinocytes: proliferating versus differentiated state.
J. Cell. Biochem. 93:1048-1062(2004)

PubMed=21269460; DOI=10.1186/1752-0509-5-17
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.
Initial characterization of the human central proteome.
BMC Syst. Biol. 5:17-17(2011)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

PubMed=26989177; DOI=10.1242/dev.133728
Elbediwy A., Vincent-Mistiaen Z.I., Spencer-Dene B., Stone R.K., Boeing S., Wculek S.K., Cordero J., Tan E.H., Ridgway R., Brunton V.G., Sahai E., Gerhardt H., Behrens A., Malanchi I., Sansom O.J., Thompson B.J.
Integrin signalling regulates YAP and TAZ to control skin homeostasis.
Development 143:1674-1687(2016)

Cross-references
Cell line collections AddexBio; T0020001/117
BCRJ; 0341
CLS; 300493/p800_HaCaT
DSMZ; ACC-771 - Discontinued
ICLC; HL14001
IZSLER; BS CL 168
KCB; KCB 200442YJ
Cell line databases/resources CLDB; cl7229
CCRID; 3111C0001CCC000373
Lonza; 56
TOKU-E; 3890
Ontologies BTO; BTO:0000552
EFO; EFO_0002056
Chemistry resources ChEMBL-Cells; CHEMBL3308509
ChEMBL-Targets; CHEMBL614392
Gene expression databases GEO; GSE57080
GEO; GSM913344
GEO; GSM913345
GEO; GSM960278
GEO; GSM960279
GEO; GSM960280
GEO; GSM960286
GEO; GSM960287
GEO; GSM960288
GEO; GSM960294
GEO; GSM960295
GEO; GSM960296
GEO; GSM1375594
GEO; GSM1375595
GEO; GSM1375596
GEO; GSM1375597
GEO; GSM1375598
GEO; GSM1375599
GEO; GSM1375600
GEO; GSM1375601
GEO; GSM1375602
GEO; GSM1375603
GEO; GSM1375604
GEO; GSM1375605
GEO; GSM1861816
GEO; GSM1861817
GEO; GSM1861820
GEO; GSM1861821
GEO; GSM2112559
GEO; GSM2112563
GEO; GSM2495796
GEO; GSM2495797
GEO; GSM2495798
Metabolomic databases MetaboLights; MTBLS127
Other Wikidata; Q5636366
Polymorphism and mutation databases Cosmic; 1530737
Proteomic databases PRIDE; PXD000496
PRIDE; PXD000670
PRIDE; PXD002460