ID   HEK293
AC   CVCL_0045
SY   Hek293; HEK-293; HEK/293; HEK 293; HEK;293; 293; 293 HEK; 293 Ad5; Human Embryonic Kidney 293
DR   BTO; BTO:0000007
DR   CLO; CLO_0001230
DR   CLO; CLO_0050903
DR   EFO; EFO_0001182
DR   MCCL; MCC:0000006
DR   CLDB; cl45
DR   CLDB; cl46
DR   CLDB; cl47
DR   CLDB; cl7133
DR   4DN; 4DNSRVF4XB1F
DR   AddexBio; T0011001/76
DR   ATCC; CRL-1573
DR   ATCC; PTA-4488
DR   BCRC; 60019
DR   BCRJ; 0009
DR   BEI_Resources; NR-9313
DR   BioSample; SAMN03471398
DR   BioSample; SAMN03473347
DR   BioSamples; SAMEA2018185
DR   CCLV; CCLV-RIE 0197
DR   CCRID; 3111C0001CCC000010
DR   CCRID; 3111C0002000000005
DR   CCRID; 3131C0001000200001
DR   CCRID; 3131C0001000200018
DR   CCRID; 3142C0001000000066
DR   CCTCC; GDC0067
DR   ChEMBL-Cells; CHEMBL3307715
DR   ChEMBL-Targets; CHEMBL614818
DR   CLS; 300192/p777_HEK293
DR   Cosmic; 2520629
DR   Cosmic; 2560255
DR   DSMZ; ACC-305
DR   eagle-i; hawaii.eagle-i.net/i/0000012b-5664-9d02-2f73-b43980000000
DR   ECACC; 85120602
DR   ENCODE; ENCBS022BLS
DR   ENCODE; ENCBS037XHS
DR   ENCODE; ENCBS209KHO
DR   ENCODE; ENCBS253HIE
DR   ENCODE; ENCBS264HQL
DR   ENCODE; ENCBS375ENC
DR   ENCODE; ENCBS393ENC
DR   ENCODE; ENCBS421ENC
DR   ENCODE; ENCBS439ENC
DR   ENCODE; ENCBS479AAA
DR   ENCODE; ENCBS480AAA
DR   ENCODE; ENCBS481AAA
DR   ENCODE; ENCBS482AAA
DR   ENCODE; ENCBS483AAA
DR   ENCODE; ENCBS484AAA
DR   ENCODE; ENCBS486AAA
DR   ENCODE; ENCBS522ENC
DR   ENCODE; ENCBS523ENC
DR   ENCODE; ENCBS613RZL
DR   ENCODE; ENCBS906GPZ
DR   FCS-free; 6-2-7-1-4-3
DR   FCS-free; 6-2-42-1-8-11
DR   FCS-free; 6-2-45-1-8-3
DR   FCS-free; 6-2-46-1-8-11
DR   FCS-free; 6-2-284-1-4-3
DR   FCS-free; 6-2-345-1-4-12
DR   FCS-free; 6-2-372-1-16-3
DR   FCS-free; 6-2-381-1-4-3
DR   FCS-free; 6-2-409-1-16-12
DR   FCS-free; 6-2-421-2-4-7
DR   FCS-free; 6-2-503-1-3-3
DR   FCS-free; 6-2-533-1-4-12
DR   FCS-free; 6-2-570-3-17-1
DR   FCS-free; 6-2-586-1-18-2
DR   GEO; GSM580027
DR   GEO; GSM580028
DR   GEO; GSM635701
DR   GEO; GSM635702
DR   GEO; GSM635703
DR   GEO; GSM635704
DR   GEO; GSM651571
DR   GEO; GSM945256
DR   GEO; GSM945288
DR   GEO; GSM1374525
DR   IBRC; C10139
DR   ICLC; HTL03003
DR   IZSLER; BS CL 129
DR   JCRB; JCRB9068
DR   KCB; KCB 200408YJ
DR   KCB; KCB 200971YJ
DR   KCLB; 21573
DR   Lonza; 46
DR   MetaboLights; MTBLS102
DR   MetaboLights; MTBLS156
DR   MetaboLights; MTBLS737
DR   NCBI_Iran; C497
DR   NIH-ARP; 103-306
DR   PRIDE; PXD000589
DR   PRIDE; PXD000623
DR   PRIDE; PXD000680
DR   PRIDE; PXD000933
DR   PRIDE; PXD000953
DR   PRIDE; PXD001468
DR   PRIDE; PXD001511
DR   PRIDE; PXD001711
DR   PRIDE; PXD001863
DR   PRIDE; PXD002383
DR   PRIDE; PXD002389
DR   PRIDE; PXD002395
DR   PRIDE; PXD002601
DR   PRIDE; PXD002613
DR   PRIDE; PXD002676
DR   PRIDE; PXD003310
DR   PRIDE; PXD003370
DR   PRIDE; PXD003700
DR   PRIDE; PXD003896
DR   PRIDE; PXD005535
DR   PRIDE; PXD005955
DR   PRIDE; PXD009668
DR   PRIDE; PXD014771
DR   RCB; RCB1637
DR   TKG; TKG 0306
DR   TOKU-E; 31
DR   Wikidata; Q489618
RX   DOI=10.13005/bpj/1414;
RX   PubMed=886304;
RX   PubMed=9217065;
RX   PubMed=15218237;
RX   PubMed=21214938;
RX   PubMed=21269460;
RX   PubMed=21502043;
RX   PubMed=22278370;
RX   PubMed=23325432;
RX   PubMed=24618588;
RX   PubMed=25182477;
RX   PubMed=25807930;
RX   PubMed=25894527;
RX   PubMed=25903999;
RX   PubMed=25960936;
RX   PubMed=27233776;
RX   PubMed=27577262;
RX   PubMed=28196595;
RX   PubMed=29660373;
RX   PubMed=30260228;
WW   https://en.wikipedia.org/wiki/HEK_293_cells
WW   http://www.hek293.com/
WW   http://hek293genome.org/v2/
WW   http://www.cells-talk.com/index.php/page/copelibrary?key=HEK293
WW   http://www.proteinatlas.org/learn/cellines
WW   http://141.61.102.20/mxdb/project/show/9191407937500
WW   http://fluoview.magnet.fsu.edu/gallery/cells/hek/hekcells.html
WW   http://en.vircell.com/products/293-cell-line/
WW   http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/2/cell-lines-detail-176.html
WW   https://web.archive.org/web/20140910211526/http://webserver.mbi.ufl.edu/~shaw/293.html
WW   https://cell-map.org/
WW   http://tcpaportal.org/mclp/
WW   http://www.cellresource.cn/fdetail.aspx?id=10
WW   http://www.cellresource.cn/fdetail.aspx?id=377
WW   http://www.cellresource.cn/fdetail.aspx?id=461
WW   http://www.cellresource.cn/fdetail.aspx?id=477
WW   http://www.cellresource.cn/fdetail.aspx?id=834
CC   Part of: ENCODE project common cell types; tier 3.
CC   Part of: MD Anderson Cell Lines Project.
CC   Doubling time: ~30 hours (CLS); ~24-30 hours (DSMZ).
CC   HLA typing: A*02:01,03:01; B*07:02,07:02/35:08; C*07:02,07:02; DQA1*01:02,01:02; DQB1*06:02,06:02; DRB1*15:01,15:01 (PubMed=25960936).
CC   Transformant: NCBI_TaxID; 28285; Adenovirus 5.
CC   Omics: Cell surface proteome.
CC   Omics: Deep antibody staining analysis.
CC   Omics: Deep proteome analysis.
CC   Omics: Deep RNAseq analysis.
CC   Omics: Genome sequenced.
CC   Omics: DNA methylation analysis.
CC   Omics: H3K4me3 ChIP-seq epigenome analysis.
CC   Omics: Metabolome analysis.
CC   Omics: Methylated arginine analysis by proteomics.
CC   Omics: Myristoylated proteins analysis by proteomics.
CC   Omics: Protein expression by reverse-phase protein arrays.
CC   Omics: Transcriptome analysis.
CC   Omics: Virome analysis using proteomics.
CC   Misspelling: HECK293; Occasionally.
CC   Misspelling: HEK239; Occasionally.
ST   Source(s): ATCC; CCRID; CLS; DSMZ; ECACC; JCRB; KCLB; PubMed=11416159; PubMed=15218237; RCB; TKG
ST   Amelogenin: X
ST   CSF1PO: 11,12
ST   D13S317: 12,14 (ATCC; CCRID; CLS; DSMZ; ECACC; KCLB; PubMed=11416159; PubMed=15218237; RCB)
ST   D13S317: 12 (JCRB; TKG)
ST   D16S539: 9,13 (ATCC; CCRID; CLS; ECACC; JCRB; RCB; TKG)
ST   D16S539: 9 (DSMZ)
ST   D18S51: 17,18 (CCRID; PubMed=11416159)
ST   D18S51: 18 (CLS)
ST   D18S51: 17 (PubMed=15218237)
ST   D19S433: 15,18 (CCRID)
ST   D19S433: 18 (CLS)
ST   D21S11: 28,30.2
ST   D2S1338: 19
ST   D3S1358: 15,17
ST   D5S818: 8,9
ST   D7S820: 11,12
ST   D8S1179: 12,14
ST   FGA: 23
ST   Penta D: 9,10
ST   Penta E: 7,15
ST   TH01: 7,9.3
ST   TPOX: 11
ST   vWA: 16,19
OX   NCBI_TaxID=9606; ! Homo sapiens
SX   Female
AG   Fetus
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 02-07-20; Version: 32
//
RX   DOI=10.13005/bpj/1414;
RA   Yuan J., Xu W.W.-W., Jiang S.Y.-L., Yu H.X.-Y., Poon H.-F.;
RT   "The scattered twelve tribes of HEK293.";
RL   Biomed. Pharmacol. J. 11:621-623(2018).
//
RX   PubMed=886304; DOI=10.1099/0022-1317-36-1-59;
RA   Graham F.L., Smiley J., Russell W.C., Nairn R.;
RT   "Characteristics of a human cell line transformed by DNA from human
RT   adenovirus type 5.";
RL   J. Gen. Virol. 36:59-74(1977).
//
RX   PubMed=9217065; DOI=10.1006/viro.1997.8597;
RA   Louis N., Evelegh C., Graham F.L.;
RT   "Cloning and sequencing of the cellular-viral junctions from the human
RT   adenovirus type 5 transformed 293 cell line.";
RL   Virology 233:423-429(1997).
//
RX   PubMed=15218237; DOI=10.1159/000078556;
RA   Bylund L., Kytola S., Lui W.-O., Larsson C., Weber G.;
RT   "Analysis of the cytogenetic stability of the human embryonal kidney
RT   cell line 293 by cytogenetic and STR profiling approaches.";
RL   Cytogenet. Genome Res. 106:28-32(2004).
//
RX   PubMed=21214938; DOI=10.1186/1471-2164-12-14;
RA   Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.;
RT   "Expression of G protein-coupled receptors and related proteins in
RT   HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray
RT   analysis.";
RL   BMC Genomics 12:14-14(2011).
//
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
//
RX   PubMed=21502043;
RA   Rubino M.J.;
RT   "Experiences with HEK293: a human cell line.";
RL   PDA J. Pharm. Sci. Technol. 64:392-395(2010).
//
RX   PubMed=22278370; DOI=10.1074/mcp.M111.014050;
RA   Geiger T., Wehner A., Schaab C., Cox J., Mann M.;
RT   "Comparative proteomic analysis of eleven common cell lines reveals
RT   ubiquitous but varying expression of most proteins.";
RL   Mol. Cell. Proteomics 11:M111.014050-M111.014050(2012).
//
RX   PubMed=23325432; DOI=10.1101/gr.147942.112;
RA   Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E.,
RA   Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A.,
RA   Crawford G.E., Absher D.M., Wold B.J., Myers R.M.;
RT   "Dynamic DNA methylation across diverse human cell lines and
RT   tissues.";
RL   Genome Res. 23:555-567(2013).
//
RX   PubMed=24618588; DOI=10.1371/journal.pone.0091433;
RA   Chernobrovkin A.L., Zubarev R.A.;
RT   "Detection of viral proteins in human cells lines by xeno-proteomics:
RT   elimination of the last valid excuse for not testing every cellular
RT   proteome dataset for viral proteins.";
RL   PLoS ONE 9:E91433-E91433(2014).
//
RX   PubMed=25182477; DOI=10.1038/ncomms5767;
RA   Lin Y.-C., Boone M., Meuris L., Lemmens I., Van Roy N., Soete A.,
RA   Reumers J., Moisse M., Plaisance S., Drmanac R., Chen J., Speleman F.,
RA   Lambrechts D., Van de Peer Y., Tavernier J., Callewaert N.;
RT   "Genome dynamics of the human embryonic kidney 293 lineage in response
RT   to cell biology manipulations.";
RL   Nat. Commun. 5:4767-4767(2014).
//
RX   PubMed=25807930; DOI=10.1002/anie.201500342;
RA   Broncel M., Serwa R.A., Ciepla P., Krause E., Dallman M.J.,
RA   Magee A.I., Tate E.W.;
RT   "Multifunctional reagents for quantitative proteome-wide analysis of
RT   protein modification in human cells and dynamic profiling of protein
RT   lipidation during vertebrate development.";
RL   Angew. Chem. Int. Ed. 54:5948-5951(2015).
//
RX   PubMed=25894527; DOI=10.1371/journal.pone.0121314;
RA   Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F.,
RA   Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R.,
RA   Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P.,
RA   Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.;
RT   "A mass spectrometric-derived cell surface protein atlas.";
RL   PLoS ONE 10:E0121314-E0121314(2015).
//
RX   PubMed=25903999; DOI=10.1002/biot.201400388;
RA   Genzel Y.;
RT   "Designing cell lines for viral vaccine production: where do we
RT   stand?";
RL   Biotechnol. J. 10:728-740(2015).
//
RX   PubMed=25960936; DOI=10.4161/21624011.2014.954893;
RA   Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.;
RT   "A catalog of HLA type, HLA expression, and neo-epitope candidates in
RT   human cancer cell lines.";
RL   OncoImmunology 3:E954893-E954893(2014).
//
RX   PubMed=27233776; DOI=10.1002/pmic.201500349;
RA   Lobas A.A., Karpov D.S., Kopylov A.T., Solovyeva E.M., Ivanov M.V.,
RA   Ilina I.Y., Lazarev V.N., Kuznetsova K.G., Ilgisonis E.V., Zgoda V.G.,
RA   Gorshkov M.V., Moshkovskii S.A.;
RT   "Exome-based proteogenomics of HEK-293 human cell line: coding genomic
RT   variants identified at the level of shotgun proteome.";
RL   Proteomics 16:1980-1991(2016).
//
RX   PubMed=27577262; DOI=10.1126/scisignal.aaf7329;
RA   Larsen S.C., Sylvestersen K.B., Mund A., Lyon D., Mullari M.,
RA   Madsen M.V., Daniel J.A., Jensen L.J., Nielsen M.L.;
RT   "Proteome-wide analysis of arginine monomethylation reveals widespread
RT   occurrence in human cells.";
RL   Sci. Signal. 9:Rs9-Rs9(2016).
//
RX   PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005;
RA   Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P.,
RA   Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W.,
RA   Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D.,
RA   Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B.,
RA   Liang H.;
RT   "Characterization of human cancer cell lines by reverse-phase protein
RT   arrays.";
RL   Cancer Cell 31:225-239(2017).
//
RX   PubMed=29660373; DOI=10.1016/j.bbagen.2018.04.012;
RA   Touat-Hamici Z., Bulteau A.-L., Bianga J., Jean-Jacques H., Szpunar J.,
RA   Lobinski R., Chavatte L.;
RT   "Selenium-regulated hierarchy of human selenoproteome in cancerous and
RT   immortalized cells lines.";
RL   Biochim. Biophys. Acta 1862:2493-2505(2018).
//
RX   PubMed=30260228; DOI=10.1021/acs.jproteome.8b00538;
RA   Knott M.E., Manzi M., Zabalegui N., Salazar M.O., Puricelli L.I.,
RA   Monge M.E.;
RT   "Metabolic footprinting of a clear cell renal cell carcinoma in vitro
RT   model for human kidney cancer detection.";
RL   J. Proteome Res. 17:3877-3888(2018).
//