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Cellosaurus HeLa S3 (CVCL_0058)

[Text version]
Cell line name HeLa S3
Synonyms HeLa s3; HeLa-S3; HELA-S3; HeLa/S3; HeLa.S3; HeLa S 3; HeLa S-3; HeLaS3; HeLa, S3 clone; S3-HeLa; S3 HeLa
Accession CVCL_0058
Resource Identification Initiative To cite this cell line use: HeLa S3 (RRID:CVCL_0058)
Comments Part of: ENCODE project common cell types; tier 2.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Population: African American.
Virology: Has at least 4 five HPV18 integration sites in the genome (PubMed=26550473).
Doubling time: ~48 hours (DSMZ=ACC-161).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: ChIP-seq epigenome analysis.
Omics: CTCF ChIP-seq epigenome analysis.
Omics: H3K27ac ChIP-seq epigenome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: H3K4me1 ChIP-seq epigenome analysis.
Omics: H3K4me2 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K79me2 ChIP-seq epigenome analysis.
Omics: H3K9ac ChIP-seq epigenome analysis.
Omics: H4K20me1 ChIP-seq epigenome analysis.
Omics: Hi-C chromosome conformation analysis.
Omics: Deep proteome analysis.
Omics: DNA methylation analysis.
Omics: Genome sequenced.
Omics: scRNAseq analysis.
Omics: Transcriptome analysis by RNAseq.
Anecdotal: The 'S' series of HeLa clones was named after Dr. Florence Sabin.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Children:
CVCL_1R87 (GM10321)CVCL_WH96 (HeLa 292)CVCL_2320 (HeLa BU-25)
CVCL_VS42 (HeLa EGFP-654)CVCL_WH97 (HeLa MR)CVCL_4W41 (HeLa PurRII7)
CVCL_2934 (HeLa S3 (sc))CVCL_ZM48 (HeLa S3 9IV)CVCL_A0DW (HeLa S3 AGO2KO)
CVCL_C8ST (HeLa S3 ATF4 KO)CVCL_C8SU (HeLa S3 ATF5 KO)CVCL_C7IT (HeLa S3 ATG3 KO)
CVCL_D2YA (HeLa S3 AZI2 KO clone 32)CVCL_D2YB (HeLa S3 AZI2 KO clone 62)CVCL_C9DV (HeLa S3 AZI2/TBKBP1 DKO clone 13)
CVCL_D2Q0 (HeLa S3 AZI2/TBKBP1 DKO clone 14)CVCL_C8SV (HeLa S3 CHOP KO by CRISPR)CVCL_C8SW (HeLa S3 CHOP KO by TALEN)
CVCL_C8SY (HeLa S3 DELE1 KO)CVCL_HP86 (HeLa S3 EGFP-alpha-tubulin/H2B-mCherry)CVCL_HP87 (HeLa S3 EGFP-H2B)
CVCL_U209 (HeLa S3 mer-)CVCL_C2VN (HeLa S3 penta KO)CVCL_D1LA (HeLa S3 RB1CC1 KO)
CVCL_D2Y9 (HeLa S3 TBKBP1 KO clone 7)CVCL_KU44 (HeLa S3 Tet-Off)CVCL_XV61 (HeLa S3/HA-E2F1)
CVCL_UM63 (HeLa-2T)CVCL_VM98 (HeLa/Fucci(CA)2)CVCL_A7AU (HeLa/Fucci(CA)5)
CVCL_A7AV (HeLa/Fucci(SA)5)CVCL_0F80 (NBR4-RJK835)CVCL_A7NW (PRHC-1)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS; DSMZ; ECACC; JCRB; KCLB; PubMed=11416159; PubMed=14518029; RCB; TKG

Markers:
AmelogeninX
CSF1PO9,10
D2S133817
D3S135815,18
D5S81811 (JCRB_IFO50011)
11,12 (ATCC; CCRID; CLS; DSMZ; ECACC; JCRB_JCRB9010; KCLB; PubMed=14518029; RCB; TKG)
D7S8208 (JCRB_JCRB9010)
8,12 (ATCC; CCRID; CLS; DSMZ; ECACC; JCRB_IFO50011; KCLB; PubMed=14518029; RCB; TKG)
D8S117912,13
D13S31712 (RCB)
12,13.3 (DSMZ; JCRB_IFO50011)
13.3 (ATCC; CCRID; CLS; JCRB_JCRB9010; KCLB; TKG)
14 (ECACC; PubMed=14518029)
D16S5399,10
D18S5116
D19S43313,14
D21S1127,28
FGA18,21
Penta D8,15
Penta E7,17
TH017
TPOX8,12
vWA16,17,18 (JCRB_JCRB9010)
16,18 (ATCC; CCRID; CLS; DSMZ; ECACC; KCLB; PubMed=11416159; PubMed=14518029; RCB; TKG)
17,18 (JCRB_IFO50011)

Run an STR similarity search on this cell line
Web pages http://genome.ucsc.edu/ENCODE/protocols/cell/human/HeLa-S3_protocol.pdf
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-157.html
http://immortalcells.blogspot.ch/2011/12/hela-vs-hela-s3.html
Publications

PubMed=16589695; DOI=10.1073/pnas.41.7.432
Puck T.T., Marcus P.I.
A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of X-irradiated cells to supply conditioning factors.
Proc. Natl. Acad. Sci. U.S.A. 41:432-437(1955)

PubMed=13286432; DOI=10.1084/jem.103.2.273
Puck T.T., Marcus P.I., Cieciura S.J.
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
J. Exp. Med. 103:273-283(1956)

PubMed=13531635; DOI=10.1007/BF00340146
Pomerat C.M., Kent S.P., Logie L.C.
Irradiation of cells in tissue culture. I. Giant cell induction in strain cultures versus elements from primary explants.
Z. Zellforsch. Mikrosk. Anat. 47:158-174(1957)

PubMed=1180926; DOI=10.1016/0006-291x(75)90551-3
Fujiwara Y., Tatsumi M.
Repair of mitomycin C damage to DNA in mammalian cells and its impairment in Fanconi's anemia cells.
Biochem. Biophys. Res. Commun. 66:592-598(1975)

PubMed=978597; DOI=10.1269/jrr.17.154
Nikaido O., Horikawa M.
Analysis of the factors in determining radiosensitivity in mammalian cells by using radio-sensitive and -resistant clones isolated from HeLa S3 cells in vitro.
J. Radiat. Res. 17:154-173(1976)

PubMed=566722; DOI=10.1007/BF02616110
Lavappa K.S.
Survey of ATCC stocks of human cell lines for HeLa contamination.
In Vitro 14:469-475(1978)

PubMed=6256643; DOI=10.1038/288724a0
Day R.S. III, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Lubiniecki A.S., Girardi A.J., Galloway S.M., Bynum G.D.
Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains.
Nature 288:724-727(1980)

PubMed=6825208; DOI=10.1093/carcin/4.2.199
Yarosh D.B., Foote R.S., Mitra S., Day R.S. III
Repair of O6-methylguanine in DNA by demethylation is lacking in Mer- human tumor cell strains.
Carcinogenesis 4:199-205(1983)

PubMed=3986962; DOI=10.1093/carcin/6.4.549
Watatani M., Ikenaga M., Hatanaka T., Kinuta M., Takai S.-i., Mori T., Kondo S.
Analysis of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced DNA damage in tumor cell strains from Japanese patients and demonstration of MNNG hypersensitivity of Mer xenografts in athymic nude mice.
Carcinogenesis 6:549-553(1985)

PubMed=3756862
Maynard K., Parsons P.G.
Cross-sensitivity of methylating agents, hydroxyurea, and methotrexate in human tumor cells of the Mer- phenotype.
Cancer Res. 46:5009-5013(1986)

PubMed=3180844; DOI=10.1159/000132579
Chen T.-R.
Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes.
Cytogenet. Cell Genet. 48:19-24(1988)

PubMed=1522048; DOI=10.1007/BF02634140
Kataoka E., Honma M., Ohnishi K., Sofuni T., Mizusawa H.
Application of highly polymorphic DNA markers to the identification of HeLa cell sublines.
In Vitro Cell. Dev. Biol. Anim. 28:553-556(1992)

CLPUB00481
Kuno H., Yoshida T.
Detection of human papillomavirus types 16, 18, and 33 in cell lines derived from human genital organs by polymerase chain reaction.
Res. Commun. Inst. Ferment. Osaka 18:6-12(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

DOI=10.11418/jtca1981.17.3_101
Itami C., Ishida R., Utsumi K.R., Nakabayashi T.
Variation in three groups of HeLa cell sublines as revealed by karyotype analysis and O6-methylguaninedna-methyltransferase activity.
Tissue Cult. Res. Commun. 17:101-106(1998)

PubMed=10338214; DOI=10.1016/s0092-8674(00)80760-6
Griffith J.D., Comeau L., Rosenfield S., Stansel R.M., Bianchi A., Moss H., de Lange T.
Mammalian telomeres end in a large duplex loop.
Cell 97:503-514(1999)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=14518029; DOI=10.1002/pros.10290
van Bokhoven A., Varella-Garcia M., Korch C.T., Johannes W.U., Smith E.E., Miller H.L., Nordeen S.K., Miller G.J., Lucia M.S.
Molecular characterization of human prostate carcinoma cell lines.
Prostate 57:205-225(2003)

PubMed=19450234; DOI=10.2144/000113089
Rahbari R., Sheahan T., Modes V., Collier P., Macfarlane C.M., Badge R.M.
A novel L1 retrotransposon marker for HeLa cell line identification.
BioTechniques 46:277-284(2009)

PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)

PubMed=23925245; DOI=10.1038/nature12064
Adey A., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R.-L., Lee C., Shendure J.
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

PubMed=24185095; DOI=10.1038/nbt.2727
Burton J.N., Adey A., Patwardhan R.P., Qiu R.-L., Kitzman J.O., Shendure J.
Chromosome-scale scaffolding of de novo genome assemblies based on chromatin interactions.
Nat. Biotechnol. 31:1119-1125(2013)

PubMed=26550473; DOI=10.1186/s13742-015-0091-4
Wu L., Zhang X.-L., Zhao Z.-K., Wang L., Li B., Li G.-B., Dean M., Yu Q.-C., Wang Y.-H., Lin X.-X., Rao W.-J., Mei Z.-L., Li Y., Jiang R.-Z., Yang H., Li F.-Q., Xie G.-Y., Xu L.-Q., Wu K., Zhang J., Chen J.-H., Wang T., Kristiansen K., Zhang X.-Q., Li Y.-R., Yang H.-M., Wang J., Hou Y., Xu X.
Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells.
GigaScience 4:51.1-51.17(2015)

PubMed=30175587; DOI=10.1021/acs.jproteome.8b00392
Robin T., Bairoch A., Muller M., Lisacek F., Lane L.
Large-scale reanalysis of publicly available HeLa cell proteomics data in the context of the Human Proteome Project.
J. Proteome Res. 17:4160-4170(2018)

PubMed=30778230; DOI=10.1038/s41587-019-0037-y
Liu Y.-S., Mi Y., Mueller T., Kreibich S., Williams E.G., Van Drogen A., Borel C., Frank M., Germain P.-L., Bludau I., Mehnert M., Seifert M., Emmenlauer M., Sorg I., Bezrukov F., Sloan-Bena F., Zhou H., Dehio C., Testa G., Saez-Rodriguez J., Antonarakis S.E., Hardt W.-D., Aebersold R.
Multi-omic measurements of heterogeneity in HeLa cells across laboratories.
Nat. Biotechnol. 37:314-322(2019)

PubMed=33205894; DOI=10.15252/msb.20209946
Schwabe D., Formichetti S., Junker J.P., Falcke M., Rajewsky N.
The transcriptome dynamics of single cells during the cell cycle.
Mol. Syst. Biol. 16:e9946.1-e9946.20(2020)

Cross-references
Cell line collections (Providers) ATCC; CCL-2.2
ATCC; CRL-7924 - Discontinued
BCRC; 60101
CCTCC; GDC0193
CLS; 300384
DSMZ; ACC-161
ECACC; 87110901
ICLC; HTL95020
IZSLER; BS TCL 22
JCRB; IFO50011
JCRB; JCRB9010
KCLB; 10002.2
NCBI_Iran; C572
RCB; RCB0191
RCB; RCB1525
RCB; RCB3682
TKG; TKG 0444
Cell line databases/resources CLO; CLO_0003695
CLO; CLO_0003696
CLO; CLO_0003699
CLO; CLO_0050921
CLO; CLO_0050936
MCCL; MCC:0000220
CLDB; cl1616
CLDB; cl1617
CLDB; cl1619
CLDB; cl1620
CLDB; cl1621
CLDB; cl1622
CCRID; 1101HUM-PUMC000188
CCRID; 1102HUM-NIFDC00009
CCRID; 4201HUM-CCTCC00193
DSMZCellDive; ACC-161
Lonza; 877
TOKU-E; 1437
Anatomy/cell type resources BTO; BTO:0000568
Biological sample resources 4DN; 4DNSRGTEEYSL
BioSample; SAMN03472408
BioSample; SAMN03472767
ENCODE; ENCBS031GUL
ENCODE; ENCBS061SJE
ENCODE; ENCBS075PNA
ENCODE; ENCBS094KYE
ENCODE; ENCBS113ENC
ENCODE; ENCBS146YHK
ENCODE; ENCBS163VBG
ENCODE; ENCBS199XIN
ENCODE; ENCBS229UDI
ENCODE; ENCBS254ZSR
ENCODE; ENCBS292TEU
ENCODE; ENCBS352IHZ
ENCODE; ENCBS376OKH
ENCODE; ENCBS403WXR
ENCODE; ENCBS417UAY
ENCODE; ENCBS423QWP
ENCODE; ENCBS424JTA
ENCODE; ENCBS432ENC
ENCODE; ENCBS434SUM
ENCODE; ENCBS450RLH
ENCODE; ENCBS468RLW
ENCODE; ENCBS493MKF
ENCODE; ENCBS500SDW
ENCODE; ENCBS529RSF
ENCODE; ENCBS575PET
ENCODE; ENCBS577WSP
ENCODE; ENCBS580FYL
ENCODE; ENCBS585KIN
ENCODE; ENCBS598ZWC
ENCODE; ENCBS635AAA
ENCODE; ENCBS636AAA
ENCODE; ENCBS637AAA
ENCODE; ENCBS646PGB
ENCODE; ENCBS647UNF
ENCODE; ENCBS655ARO
ENCODE; ENCBS656NDW
ENCODE; ENCBS658BKJ
ENCODE; ENCBS681BHB
ENCODE; ENCBS684AAA
ENCODE; ENCBS685AAA
ENCODE; ENCBS686AAA
ENCODE; ENCBS687AAA
ENCODE; ENCBS688AAA
ENCODE; ENCBS689AAA
ENCODE; ENCBS714AAA
ENCODE; ENCBS735AAA
ENCODE; ENCBS736AAA
ENCODE; ENCBS737AAA
ENCODE; ENCBS738AAA
ENCODE; ENCBS739AAA
ENCODE; ENCBS740AAA
ENCODE; ENCBS748HNU
ENCODE; ENCBS804AAA
ENCODE; ENCBS805AAA
ENCODE; ENCBS812AOY
ENCODE; ENCBS822AAA
ENCODE; ENCBS823AAA
ENCODE; ENCBS833ISA
ENCODE; ENCBS834AAA
ENCODE; ENCBS838AAA
ENCODE; ENCBS839AAA
ENCODE; ENCBS854BZN
ENCODE; ENCBS877WSH
ENCODE; ENCBS890POO
ENCODE; ENCBS899VUW
ENCODE; ENCBS979JZP
Chemistry resources ChEMBL-Cells; CHEMBL3886002
ChEMBL-Targets; CHEMBL4296436
PubChem_Cell_line; CVCL_0058
Encyclopedic resources Wikidata; Q54882556
Experimental variables resources EFO; EFO_0002791
Gene expression databases GEO; GSM472905
GEO; GSM472916
GEO; GSM472932
GEO; GSM733669
GEO; GSM733682
GEO; GSM733684
GEO; GSM733689
GEO; GSM733696
GEO; GSM733711
GEO; GSM733734
GEO; GSM733756
GEO; GSM733785
GEO; GSM736510
GEO; GSM736564
GEO; GSM749721
GEO; GSM749729
GEO; GSM749739
GEO; GSM798322
GEO; GSM803454
GEO; GSM803455
GEO; GSM803478
GEO; GSM803533
GEO; GSM816436
GEO; GSM816643
GEO; GSM816633
GEO; GSM822273
GEO; GSM822285
GEO; GSM822286
GEO; GSM923449
GEO; GSM935272
GEO; GSM935276
GEO; GSM935290
GEO; GSM935302
GEO; GSM935317
GEO; GSM935318
GEO; GSM935320
GEO; GSM935326
GEO; GSM935328
GEO; GSM935341
GEO; GSM935342
GEO; GSM935351
GEO; GSM935362
GEO; GSM935365
GEO; GSM935366
GEO; GSM935367
GEO; GSM935369
GEO; GSM935370
GEO; GSM935383
GEO; GSM935384
GEO; GSM935395
GEO; GSM935405
GEO; GSM935408
GEO; GSM935416
GEO; GSM935432
GEO; GSM935435
GEO; GSM935436
GEO; GSM935459
GEO; GSM935476
GEO; GSM935484
GEO; GSM935486
GEO; GSM935489
GEO; GSM935498
GEO; GSM935500
GEO; GSM935508
GEO; GSM935511
GEO; GSM935552
GEO; GSM935553
GEO; GSM935554
GEO; GSM935555
GEO; GSM935560
GEO; GSM935561
GEO; GSM935570
GEO; GSM935571
GEO; GSM935578
GEO; GSM935582
GEO; GSM935606
GEO; GSM935615
GEO; GSM935625
GEO; GSM935635
GEO; GSM935636
GEO; GSM935637
GEO; GSM935638
GEO; GSM945201
GEO; GSM945208
GEO; GSM945230
GEO; GSM945253
GEO; GSM1003480
GEO; GSM1003483
GEO; GSM1003520
GEO; GSM1003618
GEO; GSM2423737
GEO; GSM2423738
GEO; GSM4224315
GEO; GSM4226257
Polymorphism and mutation databases Cosmic; 846181
Cosmic; 1201780
Cosmic; 1324209
Proteomic databases PRIDE; PRD000146
PRIDE; PXD000212
PRIDE; PXD000674
PRIDE; PXD000964
PRIDE; PXD001200
PRIDE; PXD001385
PRIDE; PXD001592
PRIDE; PXD002436
PRIDE; PXD002802
PRIDE; PXD003346
PRIDE; PXD003709
PRIDE; PXD004655
PRIDE; PXD006322
Sequence databases dbGAP; phs000665.v9.p1
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number41