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Cellosaurus HEK293T (CVCL_0063)

Cell line name HEK293T
Synonyms Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo
Accession CVCL_0063
Resource Identification Initiative To cite this cell line use: HEK293T (RRID:CVCL_0063)
Comments Part of: ENCODE project common cell types; tier 3.
Part of: MD Anderson Cell Lines Project.
Doubling time: ~24-30 hours (DSMZ).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [tsA].
Omics: Genome sequenced.
Omics: miRNA expression profiling.
Omics: Protein expression by reverse-phase protein arrays.
Misspelling: HECK293T; Occasionally.
Misspelling: HEK239T; Occasionally.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0045 (HEK293)
Children:
CVCL_DR94 (293/ACE2)CVCL_JZ09 (293LTV)CVCL_JZ10 (293RTV)
CVCL_UE53 (293T cyno BCMA)CVCL_UE54 (293T cyno CCR6)CVCL_UE55 (293T cyno CD123)
CVCL_UE56 (293T cyno CD137)CVCL_UE58 (293T cyno DLL3)CVCL_UE57 (293T cyno LAG3)
CVCL_UE59 (293T cyno OX40)CVCL_0V13 (293T GNE)CVCL_UE34 (293T human B7H4)
CVCL_UE35 (293T human BCMA)CVCL_UE36 (293T human CCR6)CVCL_UC03 (293T human CD123)
CVCL_UE19 (293T human CD133)CVCL_UE20 (293T human CD137)CVCL_UE21 (293T human CD138)
CVCL_UE22 (293T human CD154(CD40L))CVCL_UE23 (293T human CD22)CVCL_UE24 (293T human CD33)
CVCL_UE25 (293T human CD40)CVCL_UC04 (293T human CLDN18.1)CVCL_UE18 (293T human CLDN18.2)
CVCL_UE37 (293T human CTLA4)CVCL_UE38 (293T human DLL3)CVCL_UE93 (293T human EGFR-vIII)
CVCL_UE39 (293T human Epcam)CVCL_UE40 (293T human FOLH1)CVCL_UE41 (293T human GPC3)
CVCL_UE42 (293T human LAG3)CVCL_UE43 (293T human NRP1)CVCL_UE44 (293T human OX40)
CVCL_UE45 (293T human OX40L)CVCL_UE46 (293T human PD-1)CVCL_UE47 (293T human PD-L1)
CVCL_UE48 (293T human PD-L2)CVCL_UE49 (293T human ROR1)CVCL_UE50 (293T human TIGIT)
CVCL_UE51 (293T human TIM3)CVCL_UE52 (293T human TROP2)CVCL_UE60 (293T mouse CCR6)
CVCL_UE61 (293T mouse CLDN18.2)CVCL_UE94 (293T OS8)CVCL_UE95 (293T OS8-hB7H4)
CVCL_UE96 (293T OS8-hPDL1)CVCL_RU09 (293T R-Spondin1 expressing)CVCL_1H20 (293T-CEP4SIVtat)
CVCL_LC70 (293T-S)CVCL_W645 (293T/APOBEC3G)CVCL_H376 (293T/AT1)
CVCL_JZ02 (293T/GFP-Puro)CVCL_KS35 (293T/NFkB-luc)CVCL_H377 (293T/NMU2)
CVCL_LC69 (293T/pAPtag-4)CVCL_BT05 (293Ta)CVCL_AR71 (293TGPRT+R1)
CVCL_9705 (293TsiLL)CVCL_1D85 (293TT)CVCL_0P39 (5H7)
CVCL_KS65 (CellSensor AP1-bla HEK 293T)CVCL_KS67 (CellSensor c-fos-bla HEK 293T)CVCL_KS57 (CellSensor CRE-bla HEK 293T)
CVCL_KS62 (CellSensor ISRE-bla HEK 293T)CVCL_KB26 (CellSensor NFAT-bla HEK 293T)CVCL_KS61 (CellSensor NFkB-bla HEK 293T)
CVCL_KS17 (CellSensor SBE-bla HEK 293T)CVCL_KS27 (CellSensor SIE-bla HEK 293T)CVCL_LF40 (GeneBLAzer UAS-bla HEK 293T)
CVCL_RU08 (HA-R-Spondin1-Fc 293T)CVCL_RW32 (Hana3A)CVCL_4U22 (HEK-SC-293T)
CVCL_VR11 (HEK293T GALE KO clone 4)CVCL_VR12 (HEK293T GALK1 KO clone 10)CVCL_VR13 (HEK293T GALK2 KO clone 7)
CVCL_QZ51 (HEK293T shPARG)CVCL_QW54 (HEK293T SMARCB1-/-)CVCL_1926 (HEK293T/17)
CVCL_HA71 (HEK293T(DMT1))CVCL_1H36 (HZ-293TS)CVCL_KS48 (MultiScreen HEK293T mGluR2)
CVCL_KS49 (MultiScreen HEK293T mGluR4)CVCL_RN04 (PDIS-1)CVCL_RN05 (PDIS-12)
CVCL_RN07 (PDIS-2)CVCL_RN08 (PDIS-22)CVCL_H716 (Phoenix-Ampho)
CVCL_H717 (Phoenix-Eco)CVCL_H718 (Phoenix-gp)CVCL_B489 (Plat-A)
CVCL_B488 (Plat-E)CVCL_B490 (Plat-GP)CVCL_AR73 (STAR)
CVCL_DA04 (Tau RD P301S FRET Biosensor)
Sex of cell Female
Age at sampling Fetus
Category Transformed cell line
STR profile Source(s): ATCC; DSMZ; ECACC; RCB

Markers:
AmelogeninX
CSF1PO11,12
D13S31712,14 (ATCC; DSMZ; RCB)
12 (ECACC)
D16S5399,13
D5S8188,9
D7S82011
TH017,9.3
TPOX11
vWA16,19
Web pages https://en.wikipedia.org/wiki/293T
http://genome.ucsc.edu/ENCODE/protocols/cell/human/HEK293T_Crawford_protocol.pdf
http://hek293genome.org/v2/
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/2/cell-lines-detail-153.html
https://www.fishersci.com/shop/products/hek293t-cell-line-hcl4517/nc0260915
http://www.genhunter.com/products/293t-cells.html
http://tcpaportal.org/mclp/
Publications

PubMed=3031469; DOI=10.1128/MCB.7.1.379
DuBridge R.B., Tang P., Hsia H.C., Leong P.-M., Miller J.H., Calos M.P.
Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system.
Mol. Cell. Biol. 7:379-387(1987)

PubMed=25182477; DOI=10.1038/ncomms5767
Lin Y.-C., Boone M., Meuris L., Lemmens I., Van Roy N., Soete A., Reumers J., Moisse M., Plaisance S., Drmanac R., Chen J., Speleman F., Lambrechts D., Van de Peer Y., Tavernier J., Callewaert N.
Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.
Nat. Commun. 5:4767-4767(2014)

PubMed=26694163; DOI=10.1371/journal.pone.0144924
Janiszewska J., Szaumkessel M., Kostrzewska-Poczekaj M., Bednarek K., Paczkowska J., Jackowska J., Grenman R., Szyfter K., Wierzbicka M., Giefing M., Jarmuz-Szymczak M.
Global miRNA expression profiling identifies miR-1290 as novel potential oncomiR in laryngeal carcinoma.
PLoS ONE 10:E0144924-E0144924(2015)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections AddexBio; T0011002/445
ATCC; CRL-3216
CCLV; CCLV-RIE 1018
DSMZ; ACC-635
ECACC; 12022001
ICLC; HTL04001
KCB; KCB 200744YJ
NCBI_Iran; C498
NCBI_Iran; C644
RCB; RCB2202
Cell line databases/resources CLDB; cl7154
CCRID; 3111C0001CCC000091
CCRID; 3131C0001000200017
Lonza; 504
TOKU-E; 3537
Ontologies BTO; BTO:0002181
CLO; CLO_0050894
EFO; EFO_0001082
EFO; EFO_0001184
Biological sample resources BioSample; SAMN01821609
BioSample; SAMN03473454
BioSamples; SAMEA2168958
BioSamples; SAMEA2536418
BioSamples; SAMEA2536419
eagle-i; hawaii.eagle-i.net/i/0000012b-5665-c7e2-2f73-b43980000000
ENCODE; ENCBS634AAA
Chemistry resources ChEMBL-Cells; CHEMBL3706569
ChEMBL-Targets; CHEMBL3706568
Gene expression databases GEO; GSM1008573
Other Wikidata; Q27546876
Polymorphism and mutation databases Cosmic; 1326280
Cosmic; 2440479
Proteomic databases PRIDE; PXD000593
PRIDE; PXD001062
PRIDE; PXD001165
PRIDE; PXD001609
PRIDE; PXD006698