Cellosaurus A-673 (CVCL_0080)

Cell line name A-673
Synonyms A673; RMS 1598; RMS1598
Accession CVCL_0080
Resource Identification Initiative To cite this cell line use: A-673 (RRID:CVCL_0080)
Comments Problematic cell line: Misidentified. Originally thought to be a rhabdomyosarcoma cell line but shown to be from an Ewing sarcoma (PubMed=10379870, PubMed=12606131, PubMed=14697648).
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: MD Anderson Cell Lines Project.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Doubling time: 28 hours (PubMed=25984343).
Microsatellite instability: Stable (MSS) (Sanger).
Sequence variation: Heterozygous for BRAF p.Val600Glu (ATCC).
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: H3K27ac ChIP-seq epigenome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K9me3 ChIP-seq epigenome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Ewing sarcoma (NCIt: C4817)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_JM58 (A-673 clone Asp114)
Sex of cell Female
Category Cancer cell line
STR profile Source(s): ATCC; CLS; Cosmic-CLP; ECACC; KCLB; PubMed=24312454; PubMed=25877200

Markers:
AmelogeninX
CSF1PO11,12
D13S3178,13
D16S53911
D18S5113,16
D19S43313,14
D21S1128,29 (CLS)
29,30.2 (PubMed=24312454; PubMed=25877200)
D2S133816,21
D3S135815,17 (CLS)
14 (KCLB; PubMed=24312454; PubMed=25877200)
D5S81811,12 (ATCC; Cosmic-CLP; ECACC; KCLB; PubMed=24312454; PubMed=25877200)
11 (CLS)
D7S82010,12
D8S117911,15 (CLS)
11,13 (PubMed=24312454; PubMed=25877200)
FGA20,21 (CLS)
19,20 (KCLB; PubMed=24312454; PubMed=25877200)
Penta D11,13 (CLS)
12,13 (PubMed=25877200)
Penta E12 (CLS)
10,13 (PubMed=25877200)
TH019.3
TPOX8
vWA15,18
Web pages http://tcpaportal.org/mclp/
Publications

PubMed=4357758; DOI=10.1093/jnci/51.5.1417
Giard D.J., Aaronson S.A., Todaro G.J., Arnstein P., Kersey J.H., Dosik H., Parks W.P.
In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.
J. Natl. Cancer Inst. 51:1417-1423(1973)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=8275086; DOI=10.1038/ng1193-230
Galili N., Davis R.J., Fredericks W.J., Mukhopadhyay S., Rauscher F.J. III, Emanuel B.S., Rovera G., Barr F.G.
Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma.
Nat. Genet. 5:230-235(1993)

PubMed=10379870; DOI=10.1002/(SICI)1098-2264(199907)25:3<241::AID-GCC6>3.0.CO;2-7
Roberts I., Wienberg J., Nacheva E., Grace C., Griffin D., Coleman N.
Novel method for the production of multiple colour chromosome paints for use in karyotyping by fluorescence in situ hybridisation.
Genes Chromosomes Cancer 25:241-250(1999)

PubMed=11423975; DOI=10.1038/sj.onc.1204437
Dauphinot L., De Oliveira C., Melot T., Sevenet N., Thomas V., Weissman B.E., Delattre O.
Analysis of the expression of cell cycle regulators in Ewing cell lines: EWS-FLI-1 modulates p57KIP2and c-Myc expression.
Oncogene 20:3258-3265(2001)

PubMed=11958134
Zhan X.-Q., Guan Y.-J., Li C., Chen Z.-C., Xie J.-Y., Chen P., Liang S.-P.
Differential proteomic analysis of human lung adenocarcinoma cell line A-549 and of normal cell line HBE.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 34:50-56(2002)

PubMed=12606131; DOI=10.1016/S0165-4608(02)00670-2
Martinez-Ramirez A., Rodriguez-Perales S., Melendez B., Martinez-Delgado B., Urioste M., Cigudosa J.C., Benitez J.
Characterization of the A673 cell line (Ewing tumor) by molecular cytogenetic techniques.
Cancer Genet. Cytogenet. 141:138-142(2003)

PubMed=14697648; DOI=10.1016/S0165-4608(03)00209-7
Coleman N., Roberts I.
Re: Characterization of the A673 cell line (Ewing tumor) by molecular cytogenetic techniques.
Cancer Genet. Cytogenet. 148:86-86(2004)

PubMed=19787792; DOI=10.1002/gcc.20717
Ottaviano L., Schaefer K.-L., Gajewski M., Huckenbeck W., Baldus S., Rogel U., Mackintosh C., de Alava E., Myklebost O., Kresse S.H., Meza-Zepeda L.A., Serra M., Cleton-Jansen A.-M., Hogendoorn P.C.W., Buerger H., Aigner T., Gabbert H.E., Poremba C.
Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.
Genes Chromosomes Cancer 49:40-51(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23882450; DOI=10.3389/fonc.2013.00183
Hinson A.R.P., Jones R., Crose L.E.S., Belyea B.C., Barr F.G., Linardic C.M.
Human rhabdomyosarcoma cell lines for rhabdomyosarcoma research: utility and pitfalls.
Front. Oncol. 3:183-183(2013)

PubMed=24312454; DOI=10.1371/journal.pone.0080060
May W.A., Grigoryan R.S., Keshelava N., Cabral D.J., Christensen L.L., Jenabi J., Ji L., Triche T.J., Lawlor E.R., Reynolds C.P.
Characterization and drug resistance patterns of Ewing's sarcoma family tumor cell lines.
PLoS ONE 8:E80060-E80060(2013)

PubMed=25010205; DOI=10.1371/journal.pgen.1004475
Brohl A.S., Solomon D.A., Chang W., Wang J., Song Y., Sindiri S., Patidar R., Hurd L., Chen L., Shern J.F., Liao H., Wen X., Gerard J., Kim J.-S., Lopez Guerrero J.A., Machado I., Wai D.H., Picci P., Triche T.J., Horvai A.E., Miettinen M., Wei J.S., Catchpoole D., Llombart-Bosch A., Waldman T., Khan J.
The genomic landscape of the Ewing Sarcoma family of tumors reveals recurrent STAG2 mutation.
PLoS Genet. 10:E1004475-E1004475(2014)

PubMed=25223734; DOI=10.1158/2159-8290.CD-14-0622
Tirode F., Surdez D., Ma X., Parker M., Le Deley M.C., Bahrami A., Zhang Z., Lapouble E., Grossetete-Lalami S., Rusch M., Reynaud S., Rio-Frio T., Hedlund E., Wu G., Chen X., Pierron G., Oberlin O., Zaidi S., Lemmon G., Gupta P., Vadodaria B., Easton J., Gut M., Ding L., Mardis E.R., Wilson R.K., Shurtleff S., Laurence V., Michon J., Marec-Berard P., Gut I., Downing J., Dyer M., Zhang J., Delattre O.
Genomic landscape of Ewing sarcoma defines an aggressive subtype with co-association of STAG2 and TP53 mutations.
Cancer Discov. 4:1342-1353(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074
Teicher B.A., Polley E., Kunkel M., Evans D., Silvers T.E., Delosh R.M., Laudeman J., Ogle C., Reinhart R., Selby M., Connelly J., Harris E., Monks A., Morris J.
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections ATCC; CRL-1598
ATCC; CRL-7910 - Discontinued
CLS; 300454/p491_A-673
ECACC; 85111504
KCLB; 21598
Cell line databases/resources CLDB; cl166
CCLE; A673_BONE
CCRID; 3131C0001000700006
Cosmic-CLP; 684052
GDSC; 684052
IGRhCellID; A673
LINCS_LDP; LCL-1410
Lonza; 1455
Ontologies BTO; BTO:0003768
CLO; CLO_0001605
CLO; CLO_0001606
EFO; EFO_0002106
Biological sample resources BioSample; SAMN03473282
ENCODE; ENCBS304EKV
ENCODE; ENCBS385OLC
ENCODE; ENCBS615MBV
ENCODE; ENCBS790NPQ
Chemistry resources ChEMBL-Cells; CHEMBL3307384
ChEMBL-Targets; CHEMBL614517
Gene expression databases GEO; GSM510002
GEO; GSM827304
GEO; GSM886859
GEO; GSM887924
GEO; GSM1669590
GEO; GSM1676306
GEO; GSM1701623
Polymorphism and mutation databases Cosmic; 684052
Cosmic; 1091064
Cosmic; 1097757
Cosmic; 1388901
Cosmic; 1718108
Cosmic; 1995338
Cosmic; 2228213
Cosmic; 2250469
Cosmic; 2294580