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Cellosaurus NALM-6 (CVCL_0092)

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Cell line name NALM-6
Synonyms Nalm-6; NALM 6; Nalm 6; NALM6; Nalm6; NALM-6-M1
Accession CVCL_0092
Resource Identification Initiative To cite this cell line use: NALM-6 (RRID:CVCL_0092)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: LL-100 blood cancer cell line panel.
Part of: MD Anderson Cell Lines Project.
Part of: NCI Pediatric Preclinical Testing Program (PPTP) cell line panel.
Doubling time: 25 hours (PubMed=25984343); 19 hours (PubMed=20922763); 40.2 hours (PubMed=8847894); ~36 hours (DSMZ).
Microsatellite instability: Instable (MSI-high) (PubMed=31068700; Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Derived from sampling site: Peripheral blood.
Sequence variations Heterozygous for EGFR p.Arg1068Ter (c.3202C>T) (CCLE; Cosmic-CLP).
Heterozygous for NRAS p.Ala146Thr (c.436G>A) (PubMed=16969076; CCLE; Cosmic-CLP).
Heterozygous for RARA p.Gln45Ter (c.133C>T) (CCLE; Cosmic-CLP).
HLA typing Source: PubMed=83966
Class I
HLA-AA*01,02
HLA-BB*08,15
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.12
Native American0.55
East Asian, North0
East Asian, South2.1
South Asian0
European, North75.01
European, South22.21
Disease Adult B acute lymphoblastic leukemia (NCIt: C9143)
Precursor B-cell acute lymphoblastic leukemia (ORDO: Orphanet_99860)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_8173 (BLIN-1)CVCL_8260 (LR10.6)CVCL_UJ07 (N6-1000)
CVCL_UJ08 (N6-500)CVCL_UJ06 (N6/ADR)CVCL_HE04 (NALM-6 DCLRE1C(-/-))
CVCL_HE05 (NALM-6 LIG4(-/-))CVCL_HE06 (NALM-6 MUS81(-/-))CVCL_HE07 (NALM-6 PRKDC(+/-))
CVCL_HE08 (NALM-6 RAD54L(-/-))CVCL_HE09 (NALM-6 TOP1(+/-))CVCL_HE10 (NALM-6 TOP2B(-/-))
CVCL_HE11 (NALM-6 TP53(+/-))CVCL_HE12 (NALM-6 TP53(-/-))CVCL_HE13 (NALM-6 XRCC5(+/-))
CVCL_J365 (Nalm-6-MSH+)CVCL_UD71 (Nalm-6-MSH+/TK+/-)CVCL_UD96 (Nalm-6-TK+/-)
CVCL_0U51 (NALM-6/SP-B)CVCL_UJ05 (NALM6, clone G5)CVCL_RA33 (Nalm6-eGFP-Puro)
CVCL_RA35 (Nalm6-Fluc-hNIS)CVCL_RA34 (Nalm6-Fluc-Neo/eGFP-Puro)CVCL_RA36 (Nalm6-Fluc-Puro)
CVCL_4V57 (NALM6/Clo)CVCL_8270 (PBEI)CVCL_JH08 (POLZ CD MSH-)
CVCL_M249 (POLZ KO MSH-)
Originate from same individual CVCL_WW76 ! B85
CVCL_5677 ! NALM-7
CVCL_L057 ! NALM-8
CVCL_L058 ! NALM-9
CVCL_L059 ! NALM-10
CVCL_L060 ! NALM-11
CVCL_5660 ! NALM-12
CVCL_L061 ! NALM-13
Sex of cell Male
Age at sampling 19Y
Category Cancer cell line
STR profile Source(s): AddexBio; COG; Cosmic-CLP; DSMZ; PubMed=11416159; PubMed=20922763; PubMed=25877200; RCB; TKG

Markers:
AmelogeninX,Y
CSF1PO12 (AddexBio; COG; Cosmic-CLP; DSMZ; PubMed=25877200)
12,13 (PubMed=20922763)
13 (RCB; TKG)
D2S133817,24
D3S135816,17 (COG; PubMed=25877200)
15,16,17 (PubMed=20922763)
D5S81811 (AddexBio)
10,11,12 (COG; DSMZ; PubMed=25877200)
11,12 (Cosmic-CLP; PubMed=20922763; RCB; TKG)
D7S8208,12 (AddexBio)
10 (COG)
8,9,10 (Cosmic-CLP; DSMZ; PubMed=25877200)
8,11,12 (PubMed=20922763)
8,11 (RCB; TKG)
D8S117911,13,14 (COG; PubMed=25877200)
12,13 (PubMed=20922763)
D13S3179,11 (AddexBio)
8,9,12 (COG; DSMZ)
9,12 (Cosmic-CLP; PubMed=25877200)
9,12,13 (PubMed=20922763)
9,13 (RCB; TKG)
D16S53911 (AddexBio)
9,10,11 (COG; DSMZ)
10,11 (Cosmic-CLP; PubMed=20922763; PubMed=25877200; RCB; TKG)
D18S5112,15 (COG; PubMed=25877200)
11,12,14,15 (PubMed=20922763)
D19S43313,15 (COG)
12,13,15 (PubMed=20922763)
D21S1128,29
FGA21,22 (COG)
22 (PubMed=11416159; PubMed=25877200)
22,23 (PubMed=20922763)
Penta D8,14
Penta E11
TH018,9 (AddexBio; COG; Cosmic-CLP; DSMZ; PubMed=20922763; RCB; TKG)
8 (PubMed=11416159; PubMed=25877200)
TPOX8,10
vWA15,18 (AddexBio)
15,16 (COG; Cosmic-CLP; DSMZ; PubMed=20922763; PubMed=25877200; RCB; TKG)

Run an STR similarity search on this cell line
Web pages http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-607.html
http://www.cogcell.org/cellreqs-ncipptp.php
http://www.pptpinvitro.org/cell_lines_panel.php
http://tcpaportal.org/mclp/
Publications

CLPUB00085
Minowada J., Oshimura M., Abe S., Greaves M.F., Janossy G., Sandberg A.A.
Human leukemia cell lines. Evidence for differentiation toward T- and B-cell axis within a leukemia clone.
Proc. Am. Assoc. Cancer Res. 19:109-109(1978)

PubMed=155647
Han T., Minowada J.
Use of stimulating capacity of mixed lymphocyte reaction (MLR-S) as a possible marker for the cell-origin of null-cell acute lymphoblastic leukaemia.
Immunology 35:333-339(1978)

PubMed=83966; DOI=10.1002/ijc.2910230206
Hurwitz R., Hozier J., LeBien T.W., Minowada J., Gajl-Peczalska K., Kubonishi I., Kersey J.
Characterization of a leukemic cell line of the pre-B phenotype.
Int. J. Cancer 23:174-180(1979)

PubMed=2415623
Goldmacher V.S., Lambert J.M., Young A.Y., Anderson J., Tinnel N.L., Kornacki M., Ritz J., Blattler W.A.
Expression of the common acute lymphoblastic leukemia antigen (CALLA) on the surface of individual cells of human lymphoblastoid lines.
J. Immunol. 136:320-325(1986)

PubMed=2783384
Luo Y., Hara H., Haruta Y., Seon B.K.
Establishment of ascitic tumor of human pre-B acute lymphoblastic leukemia in nonconditioned nude mice.
Cancer Res. 49:706-710(1989)

PubMed=2140233; DOI=10.1111/j.1440-1827.1990.tb01549.x
Nakano A., Harada T., Morikawa S., Kato Y.
Expression of leukocyte common antigen (CD45) on various human leukemia/lymphoma cell lines.
Acta Pathol. Jpn. 40:107-115(1990)

PubMed=8847894
Tani A., Tatsumi E., Nakamura F., Kumagai S., Kosaka Y., Sano K., Nakamura H., Amakawa R., Ohno H.
Sensitivity to dexamethasone and absence of bcl-2 protein in Burkitt's lymphoma cell line (Black93) derived from a patient with acute tumor lysis syndrome: comparative study with other BL and non-BL lines.
Leukemia 10:1592-1603(1996)

PubMed=9067587; DOI=10.1038/sj.leu.2400571
Uphoff C.C., MacLeod R.A.F., Denkmann S.A., Golub T.R., Borkhardt A., Janssen J.W.G., Drexler H.G.
Occurrence of TEL-AML1 fusion resulting from (12;21) translocation in human early B-lineage leukemia cell lines.
Leukemia 11:441-447(1997)

PubMed=9510473; DOI=10.1111/j.1349-7006.1998.tb00476.x
Hosoya N., Hangaishi A., Ogawa S., Miyagawa K., Mitani K., Yazaki Y., Hirai H.
Frameshift mutations of the hMSH6 gene in human leukemia cell lines.
Jpn. J. Cancer Res. 89:33-39(1998)

PubMed=9680106; DOI=10.1016/S0145-2126(98)00050-2
Matsuo Y., Drexler H.G.
Establishment and characterization of human B cell precursor-leukemia cell lines.
Leuk. Res. 22:567-579(1998)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x
Takizawa J., Suzuki R., Kuroda H., Utsunomiya A., Kagami Y., Joh T., Aizawa Y., Ueda R., Seto M.
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10490826; DOI=10.1038/sj.onc.1202874
Fu X., McGrath S., Pasillas M., Nakazawa S., Kamps M.P.
EB-1, a tyrosine kinase signal transduction gene, is transcriptionally activated in the t(1;19) subset of pre-B ALL, which express oncoprotein E2a-Pbx1.
Oncogene 18:4920-4929(1999)

PubMed=10695022
Matsuo Y., Nishizaki C., Drexler H.G.
Efficient DNA fingerprinting method for the identification of cross-culture contamination of cell lines.
Hum. Cell 12:149-154(1999)

PubMed=11021758; DOI=10.1038/sj.leu.2401891
Majka M., Rozmyslowicz T., Honczarenko M., Ratajczak J., Wasik M.A., Gaulton G.N., Ratajczak M.Z.
Biological significance of the expression of HIV-related chemokine coreceptors (CCR5 and CXCR4) and their ligands by human hematopoietic cell lines.
Leukemia 14:1821-1832(2000)

DOI=10.1016/B978-0-12-221970-2.50457-5
Drexler H.G.
The leukemia-lymphoma cell line factsbook.
(In) ISBN 9780122219702; pp.1-733; Academic Press; London (2001)

PubMed=11986953; DOI=10.1038/sj.leu.2402485
O'Donnell P.H., Guo W.-X., Reynolds C.P., Maurer B.J.
N-(4-hydroxyphenyl)retinamide increases ceramide and is cytotoxic to acute lymphoblastic leukemia cell lines, but not to non-malignant lymphocytes.
Leukemia 16:902-910(2002)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Andersson A., Eden P., Lindgren D., Nilsson J., Lassen C., Heldrup J., Fontes M., Borg A., Mitelman F., Johansson B., Hoglund M., Fioretos T.
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=16405397; DOI=10.1089/dna.2006.25.19
Adachi N., So S., Iiizumi S., Nomura Y., Murai K., Yamakawa C., Miyagawa K., Koyama H.
The human pre-B cell line Nalm-6 is highly proficient in gene targeting by homologous recombination.
DNA Cell Biol. 25:19-24(2006)

PubMed=16408098; DOI=10.1038/sj.leu.2404081
Quentmeier H., MacLeod R.A.F., Zaborski M., Drexler H.G.
JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)

PubMed=16969076; DOI=10.4161/cbt.5.8.3251
Edkins S., O'Meara S., Parker A., Stevens C., Reis M., Jones S., Greenman C., Davies H., Dalgliesh G.L., Forbes S., Hunter C., Smith R., Stephens P., Goldstraw P., Nicholson A., Chan T.L., Velculescu V.E., Yuen S.T., Leung S.Y., Stratton M.R., Futreal P.A.
Recurrent KRAS codon 146 mutations in human colorectal cancer.
Cancer Biol. Ther. 5:928-932(2006)

PubMed=20103924
Adachi N., Nishijima H., Shibahara K.-I.
Gene targeting using the human Nalm-6 pre-B cell line.
Biosci. Trends 2:169-180(2008)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20922763; DOI=10.1002/pbc.22801
Kang M.H., Smith M.A., Morton C.L., Keshelava N., Houghton P.J., Reynolds C.P.
National Cancer Institute pediatric preclinical testing program: model description for in vitro cytotoxicity testing.
Pediatr. Blood Cancer 56:239-249(2011)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23596518; DOI=10.1371/journal.pone.0061189
Suzuki T., Ukai A., Honma M., Adachi N., Nohmi T.
Restoration of mismatch repair functions in human cell line Nalm-6, which has high efficiency for gene targeting.
PLoS ONE 8:E61189-E61189(2013)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=30285677; DOI=10.1186/s12885-018-4840-5
Tan K.-T., Ding L.-W., Sun Q.-Y., Lao Z.-T., Chien W., Ren X., Xiao J.-F., Loh X.-Y., Xu L., Lill M., Mayakonda A., Lin D.-C., Yang H., Koeffler H.P.
Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines.
BMC Cancer 18:940-940(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x
Quentmeier H., Pommerenke C., Dirks W.G., Eberth S., Koeppel M., MacLeod R.A.F., Nagel S., Steube K., Uphoff C.C., Drexler H.G.
The LL-100 panel: 100 cell lines for blood cancer studies.
Sci. Rep. 9:8218-8218(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

Cross-references
Cell line collections AddexBio; C0003030/5015
CLS; 300297/NA - Discontinued
DSMZ; ACC-128
NCBI_Iran; C212
RCB; RCB1933
TKG; TKG 0413
Cell line databases/resources CLDB; cl3631
CLDB; cl3632
CCLE; NALM6_HAEMATOPOIETIC_AND_LYMPHOID_TISSUE
Cell_Model_Passport; SIDM00429
Cosmic-CLP; 908158
DepMap; ACH-000938
IGRhCellID; NALM6
LINCS_LDP; LCL-1007
Lonza; 812
Ontologies BTO; BTO:0003288
CLO; CLO_0007938
CLO; CLO_0050970
EFO; EFO_0002245
Biological sample resources BioSample; SAMN03472035
BioSample; SAMN03473163
BioSample; SAMN03473413
BioSample; SAMN10988491
Chemistry resources ChEMBL-Cells; CHEMBL3307559
ChEMBL-Targets; CHEMBL614187
GDSC; 908158
PharmacoDB; NALM6_980_2019
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
ArrayExpress; E-MTAB-7721
ArrayExpress; E-MTAB-7722
GEO; GSM236797
GEO; GSM236833
GEO; GSM887347
GEO; GSM888423
GEO; GSM1670142
Other Wikidata; Q54907426
Polymorphism and mutation databases Cosmic; 801722
Cosmic; 850385
Cosmic; 889112
Cosmic; 908158
Cosmic; 919147
Cosmic; 991553
Cosmic; 998708
Cosmic; 999748
Cosmic; 1012106
Cosmic; 1037711
Cosmic; 1070706
Cosmic; 1130246
Cosmic; 1191699
Cosmic; 1483361
Cosmic; 1509198
Cosmic; 1524810
Cosmic; 2491082
Cosmic; 2649232
IARC_TP53; 21527
LiGeA; CCLE_665
Entry history
Entry creation04-Apr-2012
Last entry update12-Mar-2020
Version number34