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Cellosaurus 16HBE14o- (CVCL_0112)

Cell line name 16HBE14o-
Synonyms 16-HBE14o; 16HBE140; 16-HBEo; 16HBEo-; 16-HBE; 16HBE
Accession CVCL_0112
Resource Identification Initiative To cite this cell line use: 16HBE14o- (RRID:CVCL_0112)
Comments Characteristics: Widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of CFTR (Millipore=SCC150).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) [Note=pSVori-].
Omics: Array-based CGH.
Omics: Proteome analysis by 2D-DE/MS.
Derived from site: In situ; Lung, bronchus, epithelium; UBERON=UBERON_0002031.
Cell type: Epithelial cell of bronchus; CL=CL_0002328.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_ZW91 (HBE-1A1)CVCL_S124 (NHBE-T)
Sex of cell Male
Age at sampling 1Y
Category Transformed cell line
STR profile Source(s): Millipore

Markers:
AmelogeninX
CSF1PO11
D3S135816
D5S81811,12
D7S82011
D8S117910,11
D13S31711
D16S53912,13
D18S5114
D21S1128,30.2
FGA21,22
Penta D9
Penta E5,14
TH019.3
TPOX8
vWA16,18

Run an STR similarity search on this cell line
Web pages http://www.dartmouth.edu/~cysfib/cell-lines.html
Publications

PubMed=1282304; DOI=10.1152/ajplung.1992.263.6.L692
Haws C., Krouse M.E., Xia Y.-F., Gruenert D.C., Wine J.J.
CFTR channels in immortalized human airway cells.
Am. J. Physiol. 263:L692-L707(1992)

PubMed=7507342; DOI=10.1165/ajrcmb.10.1.7507342
Cozens A.L., Yezzi M.J., Kunzelmann K., Ohrui T., Chin L., Eng K., Finkbeiner W.E., Widdicombe J.H., Gruenert D.C.
CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells.
Am. J. Respir. Cell Mol. Biol. 10:38-47(1994)

PubMed=9614386; DOI=10.1002/(SICI)1096-9896(199803)184:3<323::AID-PATH2>3.0.CO;2-2
Meng Q.-H., Springall D.R., Bishop A.E., Morgan K., Evans T.J., Habib S., Gruenert D.C., Gyi K.M., Hodson M.E., Yacoub M.H., Polak J.M.
Lack of inducible nitric oxide synthase in bronchial epithelium: a possible mechanism of susceptibility to infection in cystic fibrosis.
J. Pathol. 184:323-331(1998)

PubMed=10498856; DOI=10.1038/sj.bjp.0702772
Goncz K.K., Feeney L., Gruenert D.C.
Differential sensitivity of normal and cystic fibrosis airway epithelial cells to epinephrine.
Br. J. Pharmacol. 128:227-233(1999)

PubMed=12711171; DOI=10.1016/S0378-5173(03)00129-7
Forbes B., Shah A., Martin G.P., Lansley A.B.
The human bronchial epithelial cell line 16HBE14o- as a model system of the airways for studying drug transport.
Int. J. Pharmaceut. 257:161-167(2003)

PubMed=15463957; DOI=10.1016/j.jcf.2004.05.040
Gruenert D.C., Willems M., Cassiman J.-J., Frizzell R.A.
Established cell lines used in cystic fibrosis research.
J. Cyst. Fibros. 3:191-196(2004)

PubMed=18441018; DOI=10.1194/jlr.M700388-JLR200
Andersson C., Al-Turkmani M.R., Savaille J.E., Alturkmani R., Katrangi W., Cluette-Brown J.E., Zaman M.M., Laposata M., Freedman S.D.
Cell culture models demonstrate that CFTR dysfunction leads to defective fatty acid composition and metabolism.
J. Lipid Res. 49:1692-1700(2008)

PubMed=28970102; DOI=10.1016/j.jprot.2017.09.013
Puglia M., Landi C., Gagliardi A., Breslin L., Armini A., Brunetti J., Pini A., Bianchi L., Bini L.
The proteome speciation of an immortalized cystic fibrosis cell line: new perspectives on the pathophysiology of the disease.
J. Proteomics 170:28-42(2018)

Cross-references
Cell line collections (Providers) Millipore; SCC150
Cell line databases/resources MCCL; MCC:0000003
TOKU-E; 4023
Anatomy/cell type resources BTO; BTO:0002734
Chemistry resources ChEMBL-Cells; CHEMBL4295404
ChEMBL-Targets; CHEMBL4296395
PubChem_Cell_line; CVCL_0112
Encyclopedic resources Wikidata; Q54582314
Gene expression databases ArrayExpress; E-MTAB-4194
Proteomic databases PRIDE; PXD006933
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number18