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Cellosaurus 2fTGH (CVCL_0115)

[Text version]

Cell line name 2fTGH
Synonyms 2FTGH; 2-fTGH
Accession CVCL_0115
Resource Identification Initiative To cite this cell line use: 2fTGH (RRID:CVCL_0115)
Comments Characteristics: 2fTGH was derived from a HPRT negative subline of HT-1080. Expression of gpt is under the control of a IFN-alpha inducible IFI6 (6-16) promoter and is thus only expressed when IFN-alpha is present in the growth medium.
Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
Transfected with: UniProtKB; P0A9M5; E.coli gpt.
Transfected with: UniProtKB; P00557; E.coli hygromycin-B 4-O-kinase (hph).
Sequence variations Gene deletion; HGNC; 1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
Mutation; HGNC; 5382; IDH1; Simple; p.Arg132Cys (c.394C>T); ClinVar=VCV000375891; Zygosity=Heterozygous (from parent cell line).
Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (from parent cell line).
Disease Fibrosarcoma (NCIt: C3043)
Fibrosarcoma (ORDO: Orphanet_2030)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0317 (HT-1080)
Children:
CVCL_A3FC (2fTGH-U1A)CVCL_M019 (2fTGH-U2A)CVCL_9469 (2fTGH-U3A)
CVCL_9470 (2fTGH-U4A)CVCL_D314 (2fTGH-U4C)CVCL_D315 (2fTGH-U5A)
CVCL_D316 (2fTGH-U6A)
Sex of cell Male
Age at sampling 35Y
Category Cancer cell line
STR profile Source(s): ECACC

Markers:
AmelogeninX,Y
CSF1PO12
D5S81811,13
D7S8209,10
D13S31712,14
D16S5399,12
TH016
TPOX8
vWA14,19

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Publications

PubMed=2513475; DOI=10.1128/MCB.9.11.4605
Pellegrini S., John J., Shearer M., Kerr I.M., Stark G.R.
Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.
Mol. Cell. Biol. 9:4605-4612(1989)

PubMed=1837150; DOI=10.1073/pnas.88.24.11455
McKendry R., John J., Flavell D., Muller M., Kerr I.M., Stark G.R.
High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons.
Proc. Natl. Acad. Sci. U.S.A. 88:11455-11459(1991)

PubMed=1906577; DOI=10.1128/MCB.11.8.4189
John J., McKendry R., Pellegrini S., Flavell D., Kerr I.M., Stark G.R.
Isolation and characterization of a new mutant human cell line unresponsive to alpha and beta interferons.
Mol. Cell. Biol. 11:4189-4195(1991)

Cross-references
Cell line collections ECACC; 12021508
Ximbio; 151484
Ontologies BTO; BTO:0005765
MCCL; MCC:0000007
Other Wikidata; Q54584472
Entry history
Entry creation04-Apr-2012
Last entry update20-May-2021
Version number17