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Cellosaurus C2C12 (CVCL_0188)

Cell line name C2C12
Synonyms C2c12; C2-C12; C12
Accession CVCL_0188
Resource Identification Initiative To cite this cell line use: C2C12 (RRID:CVCL_0188)
Comments Part of: ENCODE project mouse cell lines.
Characteristics: Myoblast cell line capable, upon starvation, of differentiating into contractile myotubes that express muscle-specific proteins.
Doubling time: ~20 hours (DSMZ).
Omics: Cell surface proteome.
Omics: Deep proteome analysis.
Omics: H3K27me3 ChIP-seq epigenome analysis.
Omics: H3K36me3 ChIP-seq epigenome analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: H3K79me2 ChIP-seq epigenome analysis.
Omics: H3K79me3 ChIP-seq epigenome analysis.
Omics: Metabolome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: C1C12; Occasionally.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C3H.
Hierarchy Parent: CVCL_6812 (C2 [Mouse myoblast])
Children:
CVCL_AS32 (C2C12 Clone 5 DYSF shRNA)CVCL_RQ02 (C2C12-Bmpr1b)CVCL_RQ03 (C2C12-R486Q-Bmpr1b)
CVCL_RQ04 (C2C12-R486W-Bmpr1b)CVCL_KS37 (C2C12/NFkB-luc)CVCL_VR09 (C2C12i)
CVCL_0189 (C2C12N)CVCL_3900 (COM-3)CVCL_3902 (INC-2)
CVCL_KW18 (PathHunter C2C12 CXCR4 beta-arrestin)CVCL_KW19 (PathHunter C2C12 CXCR5 beta-arrestin)CVCL_KW20 (PathHunter C2C12 KISS1R beta-arrestin)
CVCL_KW21 (PathHunter C2C12 TACR1 beta-arrestin)
Sex of cell Female
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/C2C12
http://php.med.unsw.edu.au/cellbiology/index.php?title=Cells_-_C2C12
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-123.html
https://web.archive.org/web/20130502024425/http://www.stanford.edu/group/blau/protocols/c2lineprotocol.html
Publications

PubMed=563524; DOI=10.1038/270725a0
Yaffe D., Saxel O.
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle.
Nature 270:725-727(1977)

PubMed=8023908; DOI=10.1152/ajpcell.1994.266.6.C1795
McMahon D.K., Anderson P.A.W., Nassar R., Bunting J.B., Saba Z., Oakeley A.E., Malouf N.N.
C2C12 cells: biophysical, biochemical, and immunocytochemical properties.
Am. J. Physiol. 266:C1795-C1802(1994)

PubMed=15596414
Burattini S., Ferri P., Battistelli M., Curci R., Luchetti F., Falcieri E.
C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization.
Eur. J. Histochem. 48:223-233(2004)

PubMed=24735950; DOI=10.1016/j.scr.2014.03.004
Willkomm L., Schubert S., Jung R., Elsen M., Borde J., Gehlert S., Suhr F., Bloch W.
Lactate regulates myogenesis in C2C12 myoblasts in vitro.
Stem Cell Res. 12:742-753(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847-847(2014)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

Cross-references
Cell line collections AddexBio; P0028001/4665
ATCC; CRL-1772
BCRC; 60083
BCRJ; 0058
CLS; 400476/p732_C2C12
DSMZ; ACC-565
ECACC; 91031101
IZSLER; BS CL 148
KCB; KCB 2012115YJ
NCBI_Iran; C521
RCB; RCB0987
Cell line databases/resources CLDB; cl563
CLDB; cl564
CLDB; cl5172
CCRID; 3111C0001CCC000099
CCRID; 3142C0001000000143
Lonza; 692
TOKU-E; 728
Ontologies BTO; BTO:0000165
CLO; CLO_0002071
CLO; CLO_0050871
EFO; EFO_0001098
MCCL; MCC:0000079
Biological sample resources ENCODE; ENCBS124ENC
ENCODE; ENCBS299ZOZ
ENCODE; ENCBS981MXJ
Chemistry resources ChEMBL-Cells; CHEMBL3308003
ChEMBL-Targets; CHEMBL612655
Gene expression databases GEO; GSE30192
GEO; GSM915162
GEO; GSM915166
GEO; GSM915174
GEO; GSM915179
GEO; GSM915182
GEO; GSM915186
GEO; GSM915188
GEO; GSM918408
GEO; GSM918410
GEO; GSM918411
GEO; GSM918415
GEO; GSM918417
GEO; GSM918422
GEO; GSM1354755
Metabolomic databases MetaboLights; MTBLS127
Other Wikidata; Q5008075
Proteomic databases PRIDE; PXD000022
PRIDE; PXD000288
PRIDE; PXD000328
PRIDE; PXD000589
PRIDE; PXD003211
PRIDE; PXD004151
PRIDE; PXD004960