Cellosaurus DLD-1 (CVCL_0248)

Cell line name DLD-1
Synonyms DLD 1; DLD1
Accession CVCL_0248
Resource Identification Initiative To cite this cell line use: DLD-1 (RRID:CVCL_0248)
Comments Part of: AstraZeneca Colorectal cell line (AZCL) panel.
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: MD Anderson Cell Lines Project.
Doubling time: 33 hours (PubMed=25984343); ~48 hours (DSMZ).
Microsatellite instability: Instable (MSI) (PubMed=10674020; PubMed=24042735; PubMed=25926053).
Sequence variation: APC p.Arg727Met, p.Lys993Asn, p.Ile1417fs*2 and p.Arg2166Ter (PubMed=24755471).
Sequence variation: KRAS p.Gly13Asp (PubMed=12068308; PubMed=24755471).
Sequence variation: PIK3CA p.Glu545Lys and p.Asp549Asn (PubMed=24755471).
Sequence variation: TP53 p.Ser241Phe (PubMed=16418264).
Omics: Deep RNAseq analysis.
Omics: Deep phosphoproteome analysis.
Omics: miRNA expression profiling.
Omics: N-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: DLD; In Cosmic 1571767 and Cosmic 1676737.
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_3449 (__Parent_cell_line_of_DLD-1/HCT 8/HCT 15/HRT-18)
Children: CVCL_KV91 (cAMP Hunter DLD1 ADRB3 Gs); CVCL_KV92 (cAMP Hunter DLD1 HTR7D Gs); CVCL_KV93 (cAMP Hunter DLD1 PTGER2 Gs); CVCL_9798 (DKO-1); CVCL_9799 (DKO-3); CVCL_9800 (DKO-4); CVCL_9801 (DKs-5); CVCL_9802 (DKs-8); CVCL_HD52 (DLD-1 AKT1(-/-)); CVCL_HD53 (DLD-1 AKT2(-/-)); CVCL_HD54 (DLD-1 ATG7(+/-)); CVCL_HD55 (DLD-1 BBC3(-/-)); CVCL_HD56 (DLD-1 BRCA2(+/-)); CVCL_HD57 (DLD-1 BRCA2(-/-)); CVCL_HD58 (DLD-1 CDKN1A(-/-)); CVCL_V011 (DLD-1 clone A); CVCL_V012 (DLD-1 clone D); CVCL_J250 (DLD-1 clone#1-Luc); CVCL_HD59 (DLD-1 DICER1(-/-)); CVCL_HD60 (DLD-1 FBXW7(+/-)); CVCL_HD61 (DLD-1 FBXW7(-/-)); CVCL_HD62 (DLD-1 KRAS(+/-) 1); CVCL_HE28 (DLD-1 KRAS(+/-) 2); CVCL_HD63 (DLD-1 SLC2A1(-/-)); CVCL_HD64 (DLD-1 USP7(-/-)); CVCL_1Y04 (DLD-1/delta-alpha); CVCL_4V49 (DLDSNR6); CVCL_KZ27 (PathHunter DLD1 FFAR1 beta-arrestin); CVCL_KZ28 (PathHunter DLD1 HTR6 beta-arrestin); CVCL_KZ29 (PathHunter DLD1 mPTGER4 beta-arrestin); CVCL_KZ30 (PathHunter DLD1 MTNR1A Total GPCR Internalization)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; DSMZ; ECACC; JCRB; KCLB; PubMed=11416159; PubMed=25877200; TKG

Markers:
AmelogeninX,Y
CSF1PO11,12 (AddexBio; ATCC; DSMZ; ECACC; JCRB; KCLB; PubMed=25877200)
12 (TKG)
D13S3178,11
D16S53912,13
D18S5111,17
D21S1129,32.2
D3S135817
D5S81813
D7S82010,12
D8S117915
FGA22
Penta D9,14
Penta E7,14
TH017,9.3
TPOX8,11
vWA18,19
Web pages https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm
http://www.pawefish.path.cam.ac.uk/ColonCellLineDescriptions/DLD-1.html
http://tcpaportal.org/mclp/
Publications

PubMed=6652615; DOI=10.1016/0165-4608(83)90092-4
Chen T.-R., Hay R.J., Macy M.L.
Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins.
Cancer Genet. Cytogenet. 10:351-362(1983)

PubMed=8422623; DOI=10.1002/1097-0142(19930115)71:2<315::AID-CNCR2820710208>3.0.CO;2-B
Tibbetts L.M., Doremus C.M., Tzanakakis G.N., Vezeridis M.P.
Liver metastases with 10 human colon carcinoma cell lines in nude mice and association with carcinoembryonic antigen production.
Cancer 71:315-321(1993)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9515795
Sparks A.B., Morin P.J., Vogelstein B., Kinzler K.W.
Mutational analysis of the APC/beta-catenin/Tcf pathway in colorectal cancer.
Cancer Res. 58:1130-1134(1998)

PubMed=9809040; DOI=10.1016/S0165-4608(98)00081-8
Vermeulen S.J., Chen T.-R., Speleman F., Nollet F., van Roy F.M., Mareel M.M.
Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?
Cancer Genet. Cytogenet. 107:76-79(1998)

PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3
Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.
Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.
Eur. J. Cancer 35:1724-1729(1999)

PubMed=10612807; DOI=10.1002/(SICI)1098-2264(200002)27:2<183::AID-GCC10>3.0.CO;2-P
Ghadimi B.M., Sackett D.L., Difilippantonio M.J., Schrock E., Neumann T., Jauho A., Auer G., Ried T.
Centrosome amplification and instability occurs exclusively in aneuploid, but not in diploid colorectal cancer cell lines, and correlates with numerical chromosomal aberrations.
Genes Chromosomes Cancer 27:183-190(2000)

PubMed=11226274; DOI=10.1073/pnas.041603298
Abdel-Rahman W.M., Katsura K., Rens W., Gorman P.A., Sheer D., Bicknell D.C., Bodmer W.F., Arends M.J., Wyllie A.H., Edwards P.A.W.
Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement.
Proc. Natl. Acad. Sci. U.S.A. 98:2538-2543(2001)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=12068308; DOI=10.1038/nature00766
Davies H., Bignell G.R., Cox C., Stephens P., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C., Shipley J., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=16418264; DOI=10.1073/pnas.0510146103
Liu Y., Bodmer W.F.
Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines.
Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006)

PubMed=16854228; DOI=10.1186/1476-4598-5-29
Bandres E., Cubedo E., Agirre X., Malumbres R., Zarate R., Ramirez N., Abajo A., Navarro A., Moreno I., Monzo M., Garcia-Foncillas J.
Identification by real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues.
Mol. Cancer 5:29-29(2006)

PubMed=19927377; DOI=10.1002/gcc.20730
Knutsen T., Padilla-Nash H.M., Wangsa D., Barenboim-Stapleton L., Camps J., McNeil N., Difilippantonio M.J., Ried T.
Definitive molecular cytogenetic characterization of 15 colorectal cancer cell lines.
Genes Chromosomes Cancer 49:204-223(2010)

PubMed=20606684; DOI=10.1038/sj.bjc.6605780
Bracht K., Nicholls A.M., Liu Y., Bodmer W.F.
5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.
Br. J. Cancer 103:340-346(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23272949; DOI=10.1186/1755-8794-5-66
Schlicker A., Beran G., Chresta C.M., McWalter G., Pritchard A., Weston S., Runswick S., Davenport S., Heathcote K., Castro D.A., Orphanides G., French T., Wessels L.F.A.
Subtypes of primary colorectal tumors correlate with response to targeted treatment in colorectal cell lines.
BMC Med. Genomics 5:66-66(2012)

PubMed=24042735; DOI=10.1038/oncsis.2013.35
Ahmed D., Eide P.W., Eilertsen I.A., Danielsen S.A., Eknaes M., Hektoen M., Lind G.E., Lothe R.A.
Epigenetic and genetic features of 24 colon cancer cell lines.
Oncogenesis 2:E71-E71(2013)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25841592; DOI=10.1016/j.jprot.2015.03.019
Piersma S.R., Knol J.C., de Reus I., Labots M., Sampadi B.K., Pham T.V., Ishihama Y., Verheul H.M.W., Jimenez C.R.
Feasibility of label-free phosphoproteomics and application to base-line signaling of colorectal cancer cell lines.
J. Proteomics 127:247-258(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002-7002(2015)

PubMed=26537799; DOI=10.1074/mcp.M115.051235
Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J., Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E., Tollenaar R.A., Rombouts Y., Wuhrer M.
N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.
Mol. Cell. Proteomics 15:124-140(2016)

PubMed=28192450; DOI=10.1371/journal.pone.0171435
Fasterius E., Raso C., Kennedy S., Rauch N., Lundin P., Kolch W., Uhlen M., Al-Khalili Szigyarto C.
A novel RNA sequencing data analysis method for cell line authentication.
PLoS ONE 12:E0171435-E0171435(2017)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections AddexBio; C0009007/36
ATCC; CCL-221
BCRC; 60132
DSMZ; ACC-278
ECACC; 90102540
ICLC; HTL95011
JCRB; JCRB9094
KCB; KCB 201196YJ
KCLB; 10221
TKG; TKG 0379
Cell line databases/resources CLDB; cl1065
CLDB; cl1066
CCLE; DLD1_LARGE_INTESTINE
CCRID; 3131C0001000700134
ColonAtlas; DLD1
LINCS_LDP; LCL-1175
Lonza; 806
SKY/M-FISH/CGH; 1992
SKY/M-FISH/CGH; 2763
TOKU-E; 1078
Ontologies BTO; BTO:0000391
CLO; CLO_0002785
EFO; EFO_0006389
MCCL; MCC:0000138
Biological sample resources BioSample; SAMN01821551
BioSample; SAMN01821680
BioSample; SAMN03472323
Chemistry resources ChEMBL-Cells; CHEMBL3307580
ChEMBL-Targets; CHEMBL614285
Gene expression databases GEO; GSM206467
GEO; GSM274715
GEO; GSM274716
GEO; GSM274727
GEO; GSM513817
GEO; GSM514291
GEO; GSM741247
GEO; GSM886979
GEO; GSM888048
GEO; GSM1346869
GEO; GSM1374463
GEO; GSM1448161
Metabolomic databases MetaboLights; MTBLS227
Polymorphism and mutation databases Cosmic; 687519
Cosmic; 755307
Cosmic; 870451
Cosmic; 873699
Cosmic; 876713
Cosmic; 905005
Cosmic; 913890
Cosmic; 934560
Cosmic; 948123
Cosmic; 983737
Cosmic; 985994
Cosmic; 1043810
Cosmic; 1066204
Cosmic; 1122323
Cosmic; 1154643
Cosmic; 1175839
Cosmic; 1184331
Cosmic; 1184082
Cosmic; 1187305
Cosmic; 1223137
Cosmic; 1310949
Cosmic; 1466813
Cosmic; 1479591
Cosmic; 1481418
Cosmic; 1519360
Cosmic; 1524004
Cosmic; 1552176
Cosmic; 1571767
Cosmic; 1609511
Cosmic; 1676737
Cosmic; 1805251
Cosmic; 2301544
Cosmic; 2301972
Cosmic; 2464668
Cosmic; 2550352
Proteomic databases PRIDE; PXD000218
PRIDE; PXD001550