ID   FRTL-5
AC   CVCL_0265
SY   FRTL 5; FRTL5; FRTL-5 Cl 2
DR   BTO; BTO:0001875
DR   CLO; CLO_0003396
DR   MCCL; MCC:0000156
DR   CLDB; cl1395
DR   ChEMBL-Cells; CHEMBL3308088
DR   ChEMBL-Targets; CHEMBL613879
DR   CLS; 500407
DR   ECACC; 91030711
DR   PubChem_Cell_line; CVCL_0265
DR   Wikidata; Q54835180
RX   CelloPub=CLPUB00429;
RX   CelloPub=CLPUB00430;
RX   DOI=10.1128/mcb.00663-17;
RX   Patent=US4608341;
RX   PubMed=1694496;
RX   PubMed=1726925;
RX   PubMed=1846098;
RX   PubMed=2545492;
RX   PubMed=2551628;
RX   PubMed=2868604;
RX   PubMed=3475907;
RX   PubMed=3670314;
RX   PubMed=6327870;
RX   PubMed=7836899;
RX   PubMed=7912172;
RX   PubMed=8761378;
RX   PubMed=9709917;
RX   PubMed=20151827;
RX   PubMed=21087161;
CC   Group: Patented cell line.
CC   Group: Space-flown cell line (cellonaut).
CC   Problematic cell line: Probably misclassified/misidentified. ECACC has discontinued this cell line because it does not display the non-diploid karyotype originally reported therefore its authenticity cannot be assured.
CC   Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8305.
CC   Doubling time: 34 hours (CelloPub=CLPUB00429); 30 hours (PubMed=3670314).
CC   Anecdotal: Have been flown in space on the ISS in 2005 to study the effect of UVC radiation (PubMed=21087161).
CC   Discontinued: ECACC; 91030711; true.
CC   Derived from site: In situ; Thyroid gland; UBERON=UBERON_0002046.
CC   Breed/subspecies: Fischer.
OX   NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI   CVCL_5753 ! FRTL
SX   Sex unspecified
AG   6W
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 30-01-24; Version: 26
//
RX   CelloPub=CLPUB00429;
RA   Gerber H., Peter H.-J., Bosiger J., Studer H., Drews R., Ferguson D.C.,
RA   Peterson M.E., Becker D.V.;
RT   "Different continuous cell lines from adenomatous feline goiters
RT   widely differ in morphologic, functional and growth parameters.";
RL   (In) Progress in thyroid research. Proceedings of the 10th International Thyroid Conference; Gordon A., Gross J., Hennemann G. (eds.); pp.541-544; A A Balkema Publishers; Rotterdam (1991).
//
RX   CelloPub=CLPUB00430;
RA   Peter H.-J., Gerber H., Studer H., Groscurth P., Zakarija M.;
RT   "Thyroid cell lines forming follicle-like lumina in vitro.";
RL   (In) Progress in thyroid research. Proceedings of the 10th International Thyroid Conference; Gordon A., Gross J., Hennemann G. (eds.); pp.579-582; A A Balkema Publishers; Rotterdam (1991).
//
RX   DOI=10.1128/mcb.00663-17;
RA   Fusco A., Berlingieri M.T., Di Fiore P.P., Portella G., Grieco M.,
RA   Vecchio G.;
RT   "Retraction for Fusco et al., 'One- and two-step transformations of rat
RT   thyroid epithelial cells by retroviral oncogenes'.";
RL   Mol. Cell. Biol. 38:e00663.17.1-e00663.17.1(2018).
//
RX   Patent=US4608341;
RA   Ambesi-Impiombato F.S.;
RT   "Living, fast-growing thyroid cell strain, FRTL-5.";
RL   Patent number US4608341, 26-Aug-1986.
//
RX   PubMed=1694496; DOI=10.1210/endo-127-1-419;
RA   Ossendorp F.A., Bruning P.F., Schuuring E.M.D., van den Brink J.A.M.,
RA   van der Heide D., de Vijlder J.J.M., de Bruin T.W.A.;
RT   "Thyrotropin dependent and independent thyroid cell lines selected
RT   from FRTL-5 derived tumors grown in nude mice.";
RL   Endocrinology 127:419-430(1990).
//
RX   PubMed=1726925;
RA   Gerber H., Peter H.-J., Asmis L., Studer H.;
RT   "Thyroid cell lines in research on goitrogenesis.";
RL   Thyroidology 3:115-118(1991).
//
RX   PubMed=1846098; DOI=10.1210/endo-128-1-211;
RA   Peter H.-J., Gerber H., Studer H., Groscurth P., Zakarija M.;
RT   "Comparison of FRTL-5 cell growth in vitro with that of
RT   xenotransplanted cells and the thyroid of the recipient mouse.";
RL   Endocrinology 128:211-219(1991).
//
RX   PubMed=2545492; DOI=10.1016/0303-7207(89)90020-8;
RA   Sugawa H., Mori T., Imura H.;
RT   "Establishment of 8-azaguanine-resistant mutants from rat thyroid cell
RT   line FRTL-5.";
RL   Mol. Cell. Endocrinol. 62:319-326(1989).
//
RX   PubMed=2551628; DOI=10.1210/endo-125-4-1783;
RA   Pang X.-P., Hershman J.M., Chung M., Pekary A.E.;
RT   "Characterization of tumor necrosis factor-alpha receptors in human
RT   and rat thyroid cells and regulation of the receptors by
RT   thyrotropin.";
RL   Endocrinology 125:1783-1788(1989).
//
RX   PubMed=2868604; DOI=10.1530/acta.0.1110054;
RA   Brandi M.L., Rotella C.M., Zonefrati R., Toccafondi R., Aloj S.M.;
RT   "Loss of adrenergic regulation of cAMP production in the FRTL-5 cell
RT   line.";
RL   Acta Endocrinol. 111:54-61(1986).
//
RX   PubMed=3475907; DOI=10.1530/acta.0.114s242;
RA   Ambesi-Impiombato F.S., Villone G.;
RT   "The FRTL-5 thyroid cell strain as a model for studies on thyroid cell
RT   growth.";
RL   Acta Endocrinol. 116 Suppl. 281:242-245(1987).
//
RX   PubMed=3670314; DOI=10.1128/mcb.7.9.3365-3370.1987;
RA   Fusco A., Berlingieri M.T., Di Fiore P.P., Portella G., Grieco M.,
RA   Vecchio G.;
RT   "One- and two-step transformations of rat thyroid epithelial cells by
RT   retroviral oncogenes.";
RL   Mol. Cell. Biol. 7:3365-3370(1987).
//
RX   PubMed=6327870; DOI=10.1677/joe.0.1010269;
RA   Bidey S.P., Chiovato L., Day A., Turmaine M., Gould R.P., Ekins R.P.,
RA   Marshall N.J.;
RT   "Evaluation of the rat thyroid cell strain FRTL-5 as an in-vitro
RT   bioassay system for thyrotrophin.";
RL   J. Endocrinol. 101:269-276(1984).
//
RX   PubMed=7836899; DOI=10.1677/joe.0.1430527;
RA   MacNeil S., Munro D.S., Metcalfe R., Cotterell S., Ruban L.N.,
RA   Davies R., Weetman A.P.;
RT   "An investigation of the ability of TSH and Graves' immunoglobulin G
RT   to increase intracellular calcium in human thyroid cells, rat FRTL-5
RT   thyroid cells and eukaryotic cells transfected with the human TSH
RT   receptor.";
RL   J. Endocrinol. 143:527-540(1994).
//
RX   PubMed=7912172; DOI=10.1111/j.1365-2265.1994.tb03017.x;
RA   Michelangeli V.P., Munro D.S., Poon C.W., Frauman A.G., Colman P.G.;
RT   "Measurement of thyroid stimulating immunoglobulins in a new cell line
RT   transfected with a functional human TSH receptor (JPO9 cells),
RT   compared with an assay using FRTL-5 cells.";
RL   Clin. Endocrinol. (Oxf.) 40:645-652(1994).
//
RX   PubMed=8761378; DOI=10.1038/bjc.1996.409;
RA   van der Kallen C.J.H., Coes J.H., van Grafhorst J.P., Schuuring E.M.D.,
RA   Ossendorp F.A., Thijssen J.H.H., Blankenstein M.A., de Bruin T.W.A.;
RT   "Dissociation of thyrotropin receptor function and thyrotropin
RT   dependency in rat thyroid tumour cell lines derived from FRTL-5.";
RL   Br. J. Cancer 74:606-612(1996).
//
RX   PubMed=9709917; DOI=10.1089/thy.1998.8.623;
RA   Tasevski V., Benn D., Peters G., Luttrell B., Simpson A.;
RT   "The Fischer rat thyroid cell line FRTL-5 exhibits a nondiploid
RT   karyotype.";
RL   Thyroid 8:623-626(1998).
//
RX   PubMed=20151827; DOI=10.1089/thy.2009.0171;
RA   Sebai H., Hovsepian S., Ristorcelli E., Aouani A., Lombardo D.,
RA   Fayet G.;
RT   "Resveratrol increases iodide trapping in the rat thyroid cell line
RT   FRTL-5.";
RL   Thyroid 20:195-203(2010).
//
RX   PubMed=21087161; DOI=10.1089/ast.2010.0461;
RA   Albi E., Ambesi-Impiombato F.S., Villani M., De Pol I., Spelat R.,
RA   Lazzarini R., Perrella G.;
RT   "Thyroid cell growth: sphingomyelin metabolism as non-invasive marker
RT   for cell damage acquired during spaceflight.";
RL   Astrobiology 10:811-820(2010).
//