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Cellosaurus HCA7 (CVCL_0289)

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Cell line name HCA7
Synonyms HCA-7; HCA 7
Accession CVCL_0289
Resource Identification Initiative To cite this cell line use: HCA7 (RRID:CVCL_0289)
Comments Part of: KuDOS 95 cell line panel.
Microsatellite instability: Instable (MSI) (PubMed=25926053).
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations APC p.Glu582fs*20 (c.1742_1743insA) and p.Thr1556fs*3 (c.4666_4667insA) (PubMed=24755471).
FBXW7 p.Arg479Ter (c.1435C>T) (PubMed=24755471).
TP53 p.Pro301fs*44 (c.898delC) (PubMed=16418264; PubMed=24755471).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_2469 (HCA7 colony 1)CVCL_2470 (HCA7 colony 11)CVCL_2471 (HCA7 colony 24)
CVCL_2472 (HCA7 colony 26)CVCL_2473 (HCA7 colony 27)CVCL_2474 (HCA7 colony 29)
CVCL_2475 (HCA7 colony 3)CVCL_2476 (HCA7 colony 30)CVCL_2477 (HCA7 colony 6)
Sex of cell Female
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): AddexBio; ECACC; PubMed=25877200; PubMed=25926053

Markers:
AmelogeninX
CSF1PO11,14
D3S135815,19
D5S8189,12 (AddexBio)
8,12 (ECACC; PubMed=25877200)
9,11,12 (PubMed=25926053)
D7S82011,12
D8S117912,14
D13S3179
D16S53910,11 (AddexBio; ECACC; PubMed=25926053)
11 (PubMed=25877200)
D18S5119
D21S1126,28.2 (PubMed=25877200)
26,28.2,29.2 (PubMed=25926053)
FGA18.2,22
Penta D9
Penta E12,18
TH016,7
TPOX8,10
vWA14,16

Run an STR similarity search on this cell line
Web pages http://www.pawefish.path.cam.ac.uk/ColonCellLineDescriptions/HCA7.html
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-584.html
Publications

PubMed=4016751
Kirkland S.C.
Dome formation by a human colonic adenocarcinoma cell line (HCA-7).
Cancer Res. 45:3790-3795(1985)

PubMed=3718830; DOI=10.1038/bjc.1986.132
Kirkland S.C., Bailey I.G.
Establishment and characterisation of six human colorectal adenocarcinoma cell lines.
Br. J. Cancer 53:779-785(1986)

PubMed=8410493; DOI=10.1002/path.1711700407
Marsh K.A., Stamp G.W.H., Kirkland S.C.
Isolation and characterization of multiple cell types from a single human colonic carcinoma: tumourigenicity of these cell types in a xenograft system.
J. Pathol. 170:441-450(1993)

PubMed=9294210; DOI=10.1073/pnas.94.19.10330
Ilyas M., Tomlinson I.P.M., Rowan A., Pignatelli M., Bodmer W.F.
Beta-catenin mutations in cell lines established from human colorectal cancers.
Proc. Natl. Acad. Sci. U.S.A. 94:10330-10334(1997)

PubMed=10737795; DOI=10.1073/pnas.97.7.3352
Rowan A.J., Lamlum H., Ilyas M., Wheeler J., Straub J., Papadopoulou A., Bicknell D.C., Bodmer W.F., Tomlinson I.P.M.
APC mutations in sporadic colorectal tumors: a mutational 'hotspot' and interdependence of the 'two hits'.
Proc. Natl. Acad. Sci. U.S.A. 97:3352-3357(2000)

PubMed=11226274; DOI=10.1073/pnas.041603298
Abdel-Rahman W.M., Katsura K., Rens W., Gorman P.A., Sheer D., Bicknell D.C., Bodmer W.F., Arends M.J., Wyllie A.H., Edwards P.A.W.
Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement.
Proc. Natl. Acad. Sci. U.S.A. 98:2538-2543(2001)

PubMed=16418264; DOI=10.1073/pnas.0510146103
Liu Y., Bodmer W.F.
Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines.
Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006)

PubMed=18258742; DOI=10.1073/pnas.0712176105
Emaduddin M., Bicknell D.C., Bodmer W.F., Feller S.M.
Cell growth, global phosphotyrosine elevation, and c-Met phosphorylation through Src family kinases in colorectal cancer cells.
Proc. Natl. Acad. Sci. U.S.A. 105:2358-2362(2008)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192
Janakiraman M., Vakiani E., Zeng Z., Pratilas C.A., Taylor B.S., Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C., Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M., Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=20606684; DOI=10.1038/sj.bjc.6605780
Bracht K., Nicholls A.M., Liu Y., Bodmer W.F.
5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.
Br. J. Cancer 103:340-346(2010)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002-7002(2015)

PubMed=29101300; DOI=10.15252/msb.20177701
Frejno M., Zenezini Chiozzi R., Wilhelm M., Koch H., Zheng R., Klaeger S., Ruprecht B., Meng C., Kramer K., Jarzab A., Heinzlmeir S., Johnstone E., Domingo E., Kerr D., Jesinghaus M., Slotta-Huspenina J., Weichert W., Knapp S., Feller S.M., Kuster B.
Pharmacoproteomic characterisation of human colon and rectal cancer.
Mol. Syst. Biol. 13:951-951(2017)

Cross-references
Cell line collections AddexBio; C0009003/40
ECACC; 06061902
Ximbio; 152451
Cell line databases/resources ColonAtlas; HCA7
Ontologies BTO; BTO:0003031
EFO; EFO_0006415
MCCL; MCC:0000179
Biological sample resources BioSample; SAMN03471988
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM741260
GEO; GSM1346872
GEO; GSM1374491
GEO; GSM1374492
GEO; GSM1448125
Other Wikidata; Q54881491
Polymorphism and mutation databases Cosmic; 876722
Cosmic; 948860
Cosmic; 985995
Cosmic; 995407
Cosmic; 1043813
Cosmic; 1223140
Cosmic; 1312301
Cosmic; 1479629
Cosmic; 1486131
Cosmic; 1805252
Cosmic; 1933017
Cosmic; 2301976
IARC_TP53; 21760
Proteomic databases PRIDE; PXD005354