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Cellosaurus HCA7 (CVCL_0289)

[Text version]
Cell line name HCA7
Synonyms HCA-7; HCA 7
Accession CVCL_0289
Resource Identification Initiative To cite this cell line use: HCA7 (RRID:CVCL_0289)
Comments Part of: KuDOS 95 cell line panel.
Microsatellite instability: Instable (MSI) (PubMed=24755471; PubMed=25926053).
Omics: Deep proteome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; 583; APC; Simple; p.Glu582fs*20 (c.1742_1743insA); Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; 16712; FBXW7; Simple; p.Arg479Ter (c.1435C>T); Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; 11998; TP53; Simple; p.Pro301Glnfs*44 (c.898delC); Zygosity=Unspecified (PubMed=16418264; PubMed=24755471).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,02:01
HLA-BB*37:01,51:01
HLA-CC*06:02,14:02
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_2469 (HCA7 colony 1)CVCL_2470 (HCA7 colony 11)CVCL_2471 (HCA7 colony 24)
CVCL_2472 (HCA7 colony 26)CVCL_2473 (HCA7 colony 27)CVCL_2474 (HCA7 colony 29)
CVCL_2475 (HCA7 colony 3)CVCL_2476 (HCA7 colony 30)CVCL_2477 (HCA7 colony 6)
Sex of cell Female
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): AddexBio; ECACC; PubMed=25877200; PubMed=25926053

Markers:
AmelogeninX
CSF1PO11,14
D3S135815,19
D5S8188,12 (ECACC; PubMed=25877200)
9,11,12 (PubMed=25926053)
9,12 (AddexBio)
D7S82011,12
D8S117912,14
D13S3179
D16S53910,11 (AddexBio; ECACC; PubMed=25926053)
11 (PubMed=25877200)
D18S5119
D21S1126,28.2 (PubMed=25877200)
26,28.2,29.2 (PubMed=25926053)
FGA18.2,22
Penta D9
Penta E12,18
TH016,7
TPOX8,10
vWA14,16

Run an STR similarity search on this cell line
Web pages https://www.cellosaurus.org/pawefish/ColonCellLineDescriptions/HCA7.html
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-584.html
Publications

PubMed=4016751
Kirkland S.C.
Dome formation by a human colonic adenocarcinoma cell line (HCA-7).
Cancer Res. 45:3790-3795(1985)

PubMed=3718830; DOI=10.1038/bjc.1986.132
Kirkland S.C., Bailey I.G.
Establishment and characterisation of six human colorectal adenocarcinoma cell lines.
Br. J. Cancer 53:779-785(1986)

PubMed=8410493; DOI=10.1002/path.1711700407
Marsh K.A., Stamp G.W.H., Kirkland S.C.
Isolation and characterization of multiple cell types from a single human colonic carcinoma: tumourigenicity of these cell types in a xenograft system.
J. Pathol. 170:441-450(1993)

PubMed=8464898; DOI=10.1073/pnas.90.7.2842
Browning M.J., Krausa P., Rowan A.J., Bicknell D.C., Bodmer J.G., Bodmer W.F.
Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
Proc. Natl. Acad. Sci. U.S.A. 90:2842-2845(1993)

PubMed=9294210; DOI=10.1073/pnas.94.19.10330
Ilyas M., Tomlinson I.P.M., Rowan A.J., Pignatelli M., Bodmer W.F.
Beta-catenin mutations in cell lines established from human colorectal cancers.
Proc. Natl. Acad. Sci. U.S.A. 94:10330-10334(1997)

PubMed=10737795; DOI=10.1073/pnas.97.7.3352
Rowan A.J., Lamlum H., Ilyas M., Wheeler J., Straub J., Papadopoulou A., Bicknell D.C., Bodmer W.F., Tomlinson I.P.M.
APC mutations in sporadic colorectal tumors: a mutational 'hotspot' and interdependence of the 'two hits'.
Proc. Natl. Acad. Sci. U.S.A. 97:3352-3357(2000)

PubMed=11226274; DOI=10.1073/pnas.041603298
Abdel-Rahman W.M., Katsura K., Rens W., Gorman P.A., Sheer D., Bicknell D.C., Bodmer W.F., Arends M.J., Wyllie A.H., Edwards P.A.W.
Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement.
Proc. Natl. Acad. Sci. U.S.A. 98:2538-2543(2001)

PubMed=16418264; DOI=10.1073/pnas.0510146103
Liu Y., Bodmer W.F.
Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines.
Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006)

PubMed=18258742; DOI=10.1073/pnas.0712176105
Emaduddin M., Bicknell D.C., Bodmer W.F., Feller S.M.
Cell growth, global phosphotyrosine elevation, and c-Met phosphorylation through Src family kinases in colorectal cancer cells.
Proc. Natl. Acad. Sci. U.S.A. 105:2358-2362(2008)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192
Janakiraman M., Vakiani E., Zeng Z.-S., Pratilas C.A., Taylor B.S., Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C.M., Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M., Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=20606684; DOI=10.1038/sj.bjc.6605780
Bracht K., Nicholls A.M., Liu Y., Bodmer W.F.
5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.
Br. J. Cancer 103:340-346(2010)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=29101300; DOI=10.15252/msb.20177701
Frejno M., Zenezini Chiozzi R., Wilhelm M., Koch H., Zheng R.-S., Klaeger S., Ruprecht B., Meng C., Kramer K., Jarzab A., Heinzlmeir S., Johnstone E., Domingo E., Kerr D., Jesinghaus M., Slotta-Huspenina J., Weichert W., Knapp S., Feller S.M., Kuster B.
Pharmacoproteomic characterisation of human colon and rectal cancer.
Mol. Syst. Biol. 13:951-951(2017)

Cross-references
Cell line collections (Providers) AddexBio; C0009003/40
ECACC; 06061902
Ximbio; 152451 - Discontinued
Cell line databases/resources MCCL; MCC:0000179
cancercelllines; CVCL_0289
ColonAtlas; HCA7
Anatomy/cell type resources BTO; BTO:0003031
Biological sample resources BioSample; SAMN03471988
Chemistry resources PharmacoDB; HCA7_445_2019
Encyclopedic resources Wikidata; Q54881491
Experimental variables resources EFO; EFO_0006415
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM741260
GEO; GSM1346872
GEO; GSM1374491
GEO; GSM1374492
GEO; GSM1448125
GEO; GSM3145708
Polymorphism and mutation databases Cosmic; 876722
Cosmic; 948860
Cosmic; 985995
Cosmic; 995407
Cosmic; 1043813
Cosmic; 1223140
Cosmic; 1312301
Cosmic; 1479629
Cosmic; 1486131
Cosmic; 1805252
Cosmic; 1933017
Cosmic; 2301976
Cosmic; 2760084
IARC_TP53; 21760
Proteomic databases PRIDE; PXD005354
PRIDE; PXD005355
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number35