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Cellosaurus HEC-1-A (CVCL_0293)

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Cell line name HEC-1-A
Synonyms Hec-1-A; HEC-1A; HEC1-A; HEC1A; Hec1A
Accession CVCL_0293
Resource Identification Initiative To cite this cell line use: HEC-1-A (RRID:CVCL_0293)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: MD Anderson Cell Lines Project.
Part of: PI3K genetic alteration cell panel (ATCC TCP-1028).
Population: Japanese.
Doubling time: 31 hours (DOI=10.1007/978-4-431-53981-0_1); 27 hours (PubMed=25984343).
Microsatellite instability: Instable (MSI) (PubMed=12714694; PubMed=31068700).
Omics: Array-based CGH.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Endometrium; UBERON=UBERON_0001295.
Sequence variations
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Asp (c.35G>A); ClinVar=VCV000012582; Zygosity=Heterozygous (PubMed=12068308; DepMap).
  • Mutation; HGNC; 7329; MSH6; Simple; p.Phe1088Serfs*2 (c.3261delC); ClinVar=VCV000089363; Zygosity=Heterozygous (DepMap).
  • Mutation; HGNC; 7329; MSH6; Simple; c.4001+1G>T; ClinVar=VCV001736936; Zygosity=Heterozygous; Note=Splice donor mutation (DepMap).
  • Mutation; HGNC; 9122; PMS2; Simple; p.Arg802Ter (c.2404C>T); ClinVar=VCV000009237; Zygosity=Heterozygous (DepMap).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Homozygous (PubMed=15053063; PubMed=15901131; DepMap).
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American1.14
East Asian, North91.32
East Asian, South6.71
South Asian0.34
European, North0.49
European, South0
Disease Endometrial adenocarcinoma (NCIt: C7359)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1274 (HEC-1)
Children:
CVCL_AR90 (HEC-1-A-OZA1)CVCL_AR91 (HEC-1-A-OZA2)CVCL_AR92 (HEC-1-A-OZA3)
CVCL_AR93 (HEC-1-A-OZA4)CVCL_AR94 (HEC-1-A-OZA5)CVCL_AR95 (HEC-1-A-OZA6)
CVCL_0294 (HEC-1-B)
Sex of cell Female
Age at sampling 71Y
Category Cancer cell line
STR profile Source(s): ATCC; Genomics_Center_BCF_Technion; IdentiCell; JCRB

Markers:
AmelogeninX
CSF1PO10,12 (ATCC; Genomics_Center_BCF_Technion; IdentiCell)
10,13,14 (JCRB)
D1S165618.3,19
D2S44111,13
D2S133818,19
D3S135815
D5S81811,14,15 (ATCC)
11,15 (Genomics_Center_BCF_Technion; IdentiCell; JCRB)
D7S8209,11
D8S117913,14
D10S124812,14
D12S39119
D13S3179,11,14 (IdentiCell)
11 (Genomics_Center_BCF_Technion; JCRB)
11,12 (ATCC)
D16S53912 (Genomics_Center_BCF_Technion; IdentiCell)
12,13 (ATCC; JCRB)
D18S5116,21
D19S43313
D21S1130,31
D22S104511,15
FGA21,22
Penta D9,12
Penta E11
TH016,7
TPOX8,9,11 (JCRB)
8,11 (ATCC; Genomics_Center_BCF_Technion; IdentiCell)
vWA18,19 (ATCC; Genomics_Center_BCF_Technion)
18,19,20 (IdentiCell)
19,20,22 (JCRB)

Run an STR similarity search on this cell line
Web pages http://www.identicell.eu/files/Sample-IdentiCell-report.pdf
https://tcpaportal.org/mclp/
Publications

PubMed=4678779
Kuramoto H.
Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line.
Acta Obstet. Gynaecol. Jpn. 19:47-58(1972)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=2436984; DOI=10.1016/0090-8258(87)90286-1
Noumoff J.S., Haydock S.W., Sachdeva R., Heyner S., Pritchard M.L.
Characteristics of cell lines derived from normal and malignant endometrial tissue.
Gynecol. Oncol. 27:141-149(1987)

PubMed=3410349; DOI=10.1016/0090-8258(88)90297-1
Noumoff J.S., Simon D., Heyner S., Farber M., Haydock S.W., Pritchard M.L.
Cytogenetics of an endometrial adenocarcinoma cell line and its implications.
Gynecol. Oncol. 31:217-222(1988)

PubMed=10381137; DOI=10.1016/s0304-3835(98)00270-5
Ohta H., Sakamoto H., Satoh K.
In vitro effects of gonadotropin-releasing hormone (GnRH) analogue on cancer cell sensitivity to cis-platinum.
Cancer Lett. 134:111-118(1998)

PubMed=12068308; DOI=10.1038/nature00766
Davies H., Bignell G.R., Cox C., Stephens P.J., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C.S., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H.F., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=12227503; DOI=10.1111/j.1749-0774.2002.tb00103.x
Kuramoto H., Hamano M., Imai M.
HEC-1 cells.
Hum. Cell 15:81-95(2002)

DOI=10.1007/978-4-431-53981-0_1
Kuramoto H., Hamano M., Imai M., Fujisawa T., Kamata Y., Arai T., Kawaguchi M.
HEC-1 cells: establishment of an in vitro experimental system in endometrial carcinoma.
(In) Cell and molecular biology of endometrial carcinoma; Kuramoto H., Nishida M. (eds.); pp.3-34; Springer; Tokyo (2003)

PubMed=12714694; DOI=10.1093/mutage/18.3.277
Yamada N.A., Castro A., Farber R.A.
Variation in the extent of microsatellite instability in human cell lines with defects in different mismatch repair genes.
Mutagenesis 18:277-282(2003)

PubMed=12893190; DOI=10.1016/s0090-8258(03)00335-4
Tanaka R., Saito T., Ashihara K., Nishimura M., Mizumoto H., Kudo R.
Three-dimensional coculture of endometrial cancer cells and fibroblasts in human placenta derived collagen sponges and expression matrix metalloproteinases in these cells.
Gynecol. Oncol. 90:297-304(2003)

PubMed=15053063
Kamata Y., Watanabe J., Hata H., Hamano M., Kuramoto H.
Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Eur. J. Gynaecol. Oncol. 25:55-60(2004)

PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
Murai Y., Hayashi S., Takahashi H., Tsuneyama K., Takano Y.
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
Pathol. Res. Pract. 201:109-115(2005)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20218740; DOI=10.1177/153303461000900207
Wang Y.-M., Yang D., Cogdell D., Hu L.-M., Xue F.-X., Broaddus R., Zhang W.
Genomic characterization of gene copy-number aberrations in endometrial carcinoma cell lines derived from endometrioid-type endometrial adenocarcinoma.
Technol. Cancer Res. Treat. 9:179-189(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017
Korch C.T., Spillman M.A., Jackson T.A., Jacobsen B.M., Murphy S.K., Lessey B.A., Jordan V.C., Bradford A.P.
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

Cross-references
Cell line collections (Providers) ATCC; HTB-112
BCRC; 60552
CLS; 305077
JCRB; JCRB1117
JCRB; NIHS0388 - Discontinued
Cell line databases/resources CLO; CLO_0003674
MCCL; MCC:0000183
cancercelllines; CVCL_0293
CCRID; 3101HUMTCHu149
Cell_Model_Passport; SIDM00595
DepMap; ACH-000954
IGRhCellID; HEC1A
Anatomy/cell type resources BTO; BTO:0002960
Biological sample resources BioSample; SAMN03471093
BioSample; SAMN03472456
BioSample; SAMN10988293
CRISP screens repositories BioGRID_ORCS_Cell_line; 421
Chemistry resources ChEMBL-Cells; CHEMBL4295424
ChEMBL-Targets; CHEMBL4296424
PharmacoDB; HEC1A_524_2019
PubChem_Cell_line; CVCL_0293
Encyclopedic resources Wikidata; Q54882246
Experimental variables resources EFO; EFO_0002191
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
GEO; GSM844555
GEO; GSM844556
GEO; GSM887069
GEO; GSM888139
Polymorphism and mutation databases Cosmic; 713474
Cosmic; 713494
Cosmic; 724853
Cosmic; 809130
Cosmic; 846177
Cosmic; 871552
Cosmic; 889115
Cosmic; 1007160
Cosmic; 1066222
Cosmic; 1177620
Cosmic; 1177685
Cosmic; 1576457
Cosmic; 1594188
Cosmic; 1622896
Cosmic; 2030464
Cosmic; 2301550
Cosmic; 2464665
Cosmic; 2646619
IARC_TP53; 1110
LiGeA; CCLE_208
Progenetix; CVCL_0293
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update30-Jan-2024
Version number39