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Cellosaurus Huh-7 (CVCL_0336)

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Cell line name Huh-7
Synonyms HuH-7; HUH-7; HuH7; Huh7; HUH7; HUH7.0; JTC-39; Japanese Tissue Culture-39
Accession CVCL_0336
Resource Identification Initiative To cite this cell line use: Huh-7 (RRID:CVCL_0336)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: ENCODE project common cell types; tier 3.
Part of: JFCR45 cancer cell line panel.
Part of: Liver Cancer Model Repository (LIMORE).
Part of: MD Anderson Cell Lines Project.
Part of: TCGA-110-CL cell line panel.
Population: Japanese.
Doubling time: 32.2 hours (PubMed=31378681); ~48 hours (CLS).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Genome sequenced.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Homozygous for KDR p.Gln472His (c.1416A>T) (PubMed=29774518).
POLD3 p.Lys70Arg (c.209A>G) (DOI=10.1101/2020.02.17.953281).
TERT c.228C>T (-124C>T); in promoter (PubMed=31378681).
Homozygous for TP53 p.Tyr220Cys (c.659A>G) (ClinVar=VCV000127819) (PubMed=8389256; PubMed=29774518; PubMed=31395879).
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.56
Native American0
East Asian, North79.68
East Asian, South13.08
South Asian0
European, North0
European, South5.69
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_U442 (B76/Huh7)CVCL_RW46 (HG23)CVCL_X943 (Huh-5-2)
CVCL_JG51 (Huh-7-Luc)CVCL_U459 (Huh-7-Lunet)CVCL_7927 (Huh-7.5)
CVCL_WJ09 (Huh-7/F24)CVCL_XE85 (Huh-7/M8)CVCL_2957 (Huh-7D12)
CVCL_LG22 (HuH-7T1)CVCL_UZ56 (Huh7 IFITM2-/-)CVCL_HA62 (HUH7-ins)
CVCL_4W53 (Huh7S1)CVCL_VN30 (OR6 [Human])
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
STR profile Source(s): CCRID; CLS; Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; PubMed=31378681; RCB; TKG

Markers:
AmelogeninX
CSF1PO11
D1S165616
D2S133819
D3S135815
D5S81812
D6S104313,15
D7S82011
D8S117914,15
D12S39120 (CLS)
20,21 (CCRID)
D13S31710 (CLS; RCB)
10,11 (CCRID; Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; PubMed=31378681; TKG)
D16S53910
D18S5115
D19S43313,14
D21S1130
FGA22,23
Penta D12
Penta E11
TH017
TPOX8,11
vWA16,18 (CCRID; Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; PubMed=31378681; RCB; TKG)
18 (CLS)

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/Huh7
http://huh7.com/
http://genome.ucsc.edu/ENCODE/protocols/cell/human/Huh-7_Crawford_protocol.pdf
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-309.html
http://tcpaportal.org/mclp/
http://www.cellresource.cn/fdetail.aspx?id=1692
http://www.cellresource.cn/fdetail.aspx?id=2172
http://www.cellresource.cn/fdetail.aspx?id=2792
Publications

PubMed=6203805; DOI=10.20772/cancersci1959.75.2_151
Nakabayashi H., Taketa K., Yamane T., Miyazaki M., Miyano K., Sato J.
Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.
Gann 75:151-158(1984)

DOI=10.1007/978-4-431-68349-0_4
Alexander J.J.
Human hepatoma cell lines.
(In) Neoplasms of the liver; Okuda K., Ishak K.G. (eds.); pp.47-56; Springer; Tokyo (1987)

DOI=10.11418/jtca1981.12.3_221
Namba M., Nakabayashi H., Doi I., Sato J., Miyazaki M.
Cellular characteristics and utilization of human hepatoma cell lines which were established in our laboratory and distributed by Japanese Cancer Research Resources Bank.
Tissue Cult. Res. Commun. 12:221-227(1993)

PubMed=8389256; DOI=10.1093/carcin/14.5.987
Hsu I.C., Tokiwa T., Bennett W., Metcalf R.A., Welsh J.A., Sun T., Harris C.C.
p53 gene mutation and integrated hepatitis B viral DNA sequences in human liver cancer cell lines.
Carcinogenesis 14:987-992(1993)

PubMed=8835345; DOI=10.1002/(SICI)1096-9071(199602)48:2<133::AID-JMV3>3.0.CO;2-A
Tsuboi S., Nagamori S., Miyazaki M., Mihara K., Fukaya K., Teruya K., Kosaka T., Tsuji T., Namba M.
Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
J. Med. Virol. 48:133-140(1996)

DOI=10.11418/jtca1981.16.3_173
Mihara K., Miyazaki M., Fushimi K., Tsuji T., Inoue Y., Fukaya K.-I., Ohashi R., Namba M.
The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
Tissue Cult. Res. Commun. 16:173-178(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9359923; DOI=10.18926/AMO/30789
Mihara K., Miyazaki M., Kondo T., Fushimi K., Tsuji T., Inoue Y., Fukaya K.-I., Ishioka C., Namba M.
Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.
Acta Med. Okayama 51:261-265(1997)

PubMed=10523694; DOI=10.3892/or.6.6.1267
Gao C., Ohashi R., Pu H., Inoue Y., Tsuji T., Miyazaki M., Namba M.
Yeast functional assay of the p53 gene status in 11 cell lines and 26 surgical specimens of human hepatocellular carcinoma.
Oncol. Rep. 6:1267-1271(1999)

PubMed=12029633; DOI=10.1053/jhep.2002.33683
Yasui K., Arii S., Zhao C., Imoto I., Ueda M., Nagai H., Emi M., Inazawa J.
TFDP1, CUL4A, and CDC16 identified as targets for amplification at 13q34 in hepatocellular carcinomas.
Hepatology 35:1476-1484(2002)

PubMed=15708988; DOI=10.1128/JVI.79.5.2689-2699.2005
Sumpter R. Jr., Loo Y.-M., Foy E., Li K., Yoneyama M., Fujita T., Lemon S.M., Gale M. Jr.
Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I.
J. Virol. 79:2689-2699(2005)

PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Nakatsu N., Yoshida Y., Yamazaki K., Nakamura T., Dan S., Fukui Y., Yamori T.
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23285155; DOI=10.1371/journal.pone.0052697
Murayama A., Sugiyama N., Yoshimura S., Ishihara-Sugano M., Masaki T., Kim S., Wakita T., Mishiro S., Kato T.
A subclone of HuH-7 with enhanced intracellular hepatitis C virus production and evasion of virus related-cell cycle arrest.
PLoS ONE 7:E52697-E52697(2012)

PubMed=23505090; DOI=10.1002/hep.26402
Wang K., Lim H.Y., Shi S., Lee J., Deng S., Xie T., Zhu Z., Wang Y., Pocalyko D., Yang W.J., Rejto P.A., Mao M., Park C.-K., Xu J.
Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.
Hepatology 58:706-717(2013)

PubMed=23887712; DOI=10.1038/ncomms3218
Nault J.-C., Mallet M., Pilati C., Calderaro J., Bioulac-Sage P., Laurent C., Laurent A., Cherqui D., Balabaud C., Zucman-Rossi J.
High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.
Nat. Commun. 4:2218-2218(2013)

PubMed=24973239; DOI=10.1099/vir.0.065995-0
Richter M., Reimann I., Schirrmeier H., Kirkland P.D., Beer M.
The viral envelope is not sufficient to transfer the unique broad cell tropism of Bungowannah virus to a related pestivirus.
J. Gen. Virol. 95:2216-2222(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25574106; DOI=10.3748/wjg.v21.i1.311
Cevik D., Yildiz G., Ozturk M.
Common telomerase reverse transcriptase promoter mutations in hepatocellular carcinomas from different geographical locations.
World J. Gastroenterol. 21:311-317(2015)

PubMed=27329724; DOI=10.18632/oncotarget.10161
Watari K., Nishitani A., Shibata T., Noda M., Kawahara A., Akiba J., Murakami Y., Yano H., Kuwano M., Ono M.
Phosphorylation of mTOR Ser2481 is a key target limiting the efficacy of rapalogs for treating hepatocellular carcinoma.
Oncotarget 7:47403-47417(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29610054; DOI=10.1016/j.dmpk.2018.03.003
Shi J., Wang X., Lyu L., Jiang H., Zhu H.-J.
Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.
Drug Metab. Pharmacokinet. 33:133-140(2018)

PubMed=29774518; DOI=10.1007/s13577-018-0212-3
Kasai F., Hirayama N., Ozawa M., Satoh M., Kohara A.
HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.
Hum. Cell 31:261-267(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31378681; DOI=10.1016/j.ccell.2019.07.001
Qiu Z.-X., Li H., Zhang Z.-T., Zhu Z.-F., He S., Wang X.-J., Wang P.-C., Qin J.-J., Zhuang L.-P., Wang W., Xie F.-B., Gu Y., Zou K.-K., Li C., Li C., Wang C.-H., Cen J., Chen X.-T., Shu Y.-J., Zhang Z., Sun L.-L., Min L.-H., Fu Y., Huang X.-W., Lv H., Zhou H., Ji Y., Zhang Z.-G., Meng Z.-Q., Shi X.-L., Zhang H.-B., Li Y.-X., Hui L.-J.
A pharmacogenomic landscape in human liver cancers.
Cancer Cell 36:179-193.e11(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2
Yu K., Chen B., Aran D., Charalel J., Yau C., Wolf D.M., van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat. Commun. 10:3574-3574(2019)

DOI=10.1101/2020.02.17.953281
Kawamoto M., Yamaji T., Saito K., Satomura K., Endo T., Fukasawa M., Hanada K., Osada N.
Identification of characteristic genomic markers in human hepatoma Huh7 and Huh7.5.1-8 cell lines.
bioRxiv 2020:953281-953281(2020)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

Cross-references
Cell line collections CCLV; CCLV-RIE 1079
CCTCC; GDC0134
CLS; 300156/p7178_HuH7
JCRB; JCRB0403
KCB; KCB 200970YJ
KCLB; 60104
RCB; RCB1366
RCB; RCB1942
TKG; TKG 0206
Cell line databases/resources CCLE; HUH7_LIVER
CCRID; 3111C0001CCC000679
CCRID; 3131C0001000700182
CCRID; 3153C0001000000221
Cell_Model_Passport; SIDM00585
CGH-DB; 453-3
CGH-DB; 9072-4
Cosmic-CLP; 907071
DepMap; ACH-000480
FCS-free; 213-2-437-1-3-3
FCS-free; 213-2-540-1-3-3
LIMORE; HuH7
LINCS_LDP; LCL-1926
Lonza; 244
TOKU-E; 3614
Ontologies BTO; BTO:0001950
CLO; CLO_0050850
CLO; CLO_0050853
EFO; EFO_0005384
MCCL; MCC:0000232
Biological sample resources BioSample; SAMN03471912
BioSample; SAMN03472130
BioSample; SAMN10987834
ENCODE; ENCBS226AAA
Chemistry resources ChEMBL-Cells; CHEMBL3307515
ChEMBL-Targets; CHEMBL614039
GDSC; 907071
PharmacoDB; HuH7_640_2019
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM207048
GEO; GSM388882
GEO; GSM388883
GEO; GSM388884
GEO; GSM481447
GEO; GSM501781
GEO; GSM565880
GEO; GSM816641
GEO; GSM827171
GEO; GSM887149
GEO; GSM888221
GEO; GSM936763
GEO; GSM1178257
GEO; GSM1178258
GEO; GSM1374566
GEO; GSM1669919
GEO; GSM2551570
Other Wikidata; Q27555640
Polymorphism and mutation databases Cosmic; 871515
Cosmic; 873395
Cosmic; 907071
Cosmic; 945154
Cosmic; 948063
Cosmic; 979730
Cosmic; 1187330
Cosmic; 1351512
Cosmic; 1518226
Cosmic; 1622751
Cosmic; 1995450
Cosmic; 2023863
Cosmic; 2162528
Cosmic; 2321028
Cosmic; 2668283
Cosmic; 2674232
IARC_TP53; 2011
LiGeA; CCLE_245
Proteomic databases PRIDE; PXD001339
PRIDE; PXD005955
PRIDE; PXD008190
Entry history
Entry creation04-Apr-2012
Last entry update02-Jul-2020
Version number34