Cellosaurus Huh-7 (CVCL_0336)

Cell line name Huh-7
Synonyms HuH-7; HUH-7; HuH7; Huh7; HUH7; JTC-39; Japanese Tissue Culture-39
Accession CVCL_0336
Resource Identification Initiative To cite this cell line use: Huh-7 (RRID:CVCL_0336)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: ENCODE project common cell types; tier 3.
Part of: JFCR45 cancer cell line panel.
Part of: MD Anderson Cell Lines Project.
Doubling time: ~48 hours (CLS).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Homozygous for KDR p.Gln472His (c.1416A>T) (PubMed=29774518).
Homozygous for TP53 p.Tyr220Cys (c.659A>G) (PubMed=8389256; PubMed=29774518).
Disease Hepatocellular carcinoma (NCIt: C3099)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_U442 (B76/Huh7)CVCL_RW46 (HG23)CVCL_X943 (Huh-5-2)
CVCL_JG51 (Huh-7-Luc)CVCL_U459 (Huh-7-Lunet)CVCL_7927 (Huh-7.5)
CVCL_2957 (Huh-7D12)CVCL_LG22 (HuH-7T1)CVCL_HA62 (HUH7-ins)
CVCL_4W53 (Huh7S1)CVCL_VN30 (OR6 [Human])
Sex of cell Male
Age at sampling 57Y
Category Cancer cell line
STR profile Source(s): CLS; Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; RCB; TKG

Markers:
AmelogeninX
CSF1PO11
D12S39120
D13S31710 (CLS; RCB)
10,11 (Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; TKG)
D16S53910
D18S5115
D19S43313,14
D1S165616
D21S1130
D2S133819
D3S135815
D5S81812
D6S104313,15
D7S82011
D8S117914,15
FGA22,23
Penta D12
Penta E11
TH017
TPOX8,11
vWA18 (CLS)
16,18 (Cosmic-CLP; JCRB; KCLB; PubMed=25877200; PubMed=29774518; RCB; TKG)
Web pages https://en.wikipedia.org/wiki/Huh7
http://huh7.com/
http://genome.ucsc.edu/ENCODE/protocols/cell/human/Huh-7_Crawford_protocol.pdf
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-309.html
http://tcpaportal.org/mclp/
Publications

PubMed=6203805; DOI=10.20772/cancersci1959.75.2_151
Nakabayashi H., Taketa K., Yamane T., Miyazaki M., Miyano K., Sato J.
Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.
Gann 75:151-158(1984)

DOI=10.1007/978-4-431-68349-0_4
Alexander J.J.
Human hepatoma cell lines.
(In) Neoplasms of the liver; Okuda K., Ishak K.G. (eds.); pp.47-56; Springer; Tokyo (1987)

PubMed=8389256; DOI=10.1093/carcin/14.5.987
Hsu I.C., Tokiwa T., Bennett W., Metcalf R.A., Welsh J.A., Sun T., Harris C.C.
p53 gene mutation and integrated hepatitis B viral DNA sequences in human liver cancer cell lines.
Carcinogenesis 14:987-992(1993)

DOI=10.11418/jtca1981.16.3_173
Mihara K., Miyazaki M., Fushimi K., Tsuji T., Inoue Y., Fukaya K.-I., Ohashi R., Namba M.
The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
Tissue Cult. Res. Commun. 16:173-178(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=12029633; DOI=10.1053/jhep.2002.33683
Yasui K., Arii S., Zhao C., Imoto I., Ueda M., Nagai H., Emi M., Inazawa J.
TFDP1, CUL4A, and CDC16 identified as targets for amplification at 13q34 in hepatocellular carcinomas.
Hepatology 35:1476-1484(2002)

PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Nakatsu N., Yoshida Y., Yamazaki K., Nakamura T., Dan S., Fukui Y., Yamori T.
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23285155; DOI=10.1371/journal.pone.0052697
Murayama A., Sugiyama N., Yoshimura S., Ishihara-Sugano M., Masaki T., Kim S., Wakita T., Mishiro S., Kato T.
A subclone of HuH-7 with enhanced intracellular hepatitis C virus production and evasion of virus related-cell cycle arrest.
PLoS ONE 7:E52697-E52697(2012)

PubMed=23505090; DOI=10.1002/hep.26402
Wang K., Lim H.Y., Shi S., Lee J., Deng S., Xie T., Zhu Z., Wang Y., Pocalyko D., Yang W.J., Rejto P.A., Mao M., Park C.-K., Xu J.
Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.
Hepatology 58:706-717(2013)

PubMed=23887712; DOI=10.1038/ncomms3218
Nault J.-C., Mallet M., Pilati C., Calderaro J., Bioulac-Sage P., Laurent C., Laurent A., Cherqui D., Balabaud C., Zucman-Rossi J.
High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.
Nat. Commun. 4:2218-2218(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25574106; DOI=10.3748/wjg.v21.i1.311
Cevik D., Yildiz G., Ozturk M.
Common telomerase reverse transcriptase promoter mutations in hepatocellular carcinomas from different geographical locations.
World J. Gastroenterol. 21:311-317(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29610054; DOI=10.1016/j.dmpk.2018.03.003
Shi J., Wang X., Lyu L., Jiang H., Zhu H.-J.
Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes.
Drug Metab. Pharmacokinet. 33:133-140(2018)

PubMed=29774518; DOI=10.1007/s13577-018-0212-3
Kasai F., Hirayama N., Ozawa M., Satoh M., Kohara A.
HuH-7 reference genome profile: complex karyotype composed of massive loss of heterozygosity.
Hum. Cell 31:261-267(2018)

Cross-references
Cell line collections CCLV; CCLV-RIE 1079
CLS; 300156/p7178_HuH7
JCRB; JCRB0403
KCB; KCB 200970YJ
KCLB; 60104
RCB; RCB1366
RCB; RCB1942
TKG; TKG 0206
Cell line databases/resources CCLE; HUH7_LIVER
CGH-DB; 453-3
CGH-DB; 9072-4
Cosmic-CLP; 907071
GDSC; 907071
LINCS_LDP; LCL-1926
Lonza; 244
TOKU-E; 3614
Ontologies BTO; BTO:0001950
CLO; CLO_0050850
CLO; CLO_0050853
EFO; EFO_0005384
MCCL; MCC:0000232
Biological sample resources BioSample; SAMN03471912
BioSample; SAMN03472130
ENCODE; ENCBS226AAA
Chemistry resources ChEMBL-Cells; CHEMBL3307515
ChEMBL-Targets; CHEMBL614039
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-3610
GEO; GSM207048
GEO; GSM388882
GEO; GSM388883
GEO; GSM388884
GEO; GSM481447
GEO; GSM565880
GEO; GSM816641
GEO; GSM827171
GEO; GSM887149
GEO; GSM888221
GEO; GSM936763
GEO; GSM1374566
GEO; GSM1669919
Other Wikidata; Q27555640
Polymorphism and mutation databases Cosmic; 871515
Cosmic; 873395
Cosmic; 907071
Cosmic; 945154
Cosmic; 948063
Cosmic; 979730
Cosmic; 1187330
Cosmic; 1351512
Cosmic; 1518226
Cosmic; 1622751
Cosmic; 1995450
Cosmic; 2023863
Cosmic; 2162528
Cosmic; 2321028
Cosmic; 2668283
Cosmic; 2674232
Proteomic databases PRIDE; PXD001339
PRIDE; PXD005955
PRIDE; PXD008190