Cellosaurus IMR-32 (CVCL_0346)

Cell line name IMR-32
Synonyms IMR 32; IMR32; Institute for Medical Research-32; GM03320
Accession CVCL_0346
Resource Identification Initiative To cite this cell line use: IMR-32 (RRID:CVCL_0346)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Doubling time: 48 hours (PubMed=5459762); ~20 hours (ATCC); ~40-50 hours (DSMZ).
Omics: Cell surface proteome.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: IMR-92; Occasionally.
Misspelling: IRM-32; Occasionally.
Disease Neuroblastoma (NCIt: C3270)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_7166 (CHP-100); CVCL_S028 (IMR-32 SimpleCell O-GalNAc); CVCL_ER17 (IMR-32_pCMV-DPP3); CVCL_ER18 (IMR-32_pCMV-SQSTM1); CVCL_1306 (IMR-5)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): ATCC; CLS; COG; DSMZ; JCRB; KCLB; PubMed=25877200; RCB; TKG

Markers:
AmelogeninX,Y
CSF1PO11,12
D13S3179
D16S5398,10 (CLS)
8 (ATCC; COG; DSMZ; JCRB; PubMed=25877200; RCB; TKG)
D18S5112,15
D19S43314,15
D21S1130,31
D2S133823,24
D3S135816
D5S81811,12
D7S8209,10
D8S117913
FGA21,24
Penta D11,12
Penta E7,15
TH017,9.3 (CLS; COG; DSMZ; JCRB_IFO50283; KCLB; PubMed=25877200; RCB; TKG)
7,10 (JCRB_JCRB9050)
TPOX11
vWA15
Web pages http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/i/cell-lines-detail-508.html
Publications

PubMed=5459762
Tumilowicz J.J., Nichols W.W., Cholon J.J., Greene A.E.
Definition of a continuous human cell line derived from neuroblastoma.
Cancer Res. 30:2110-2118(1970)

DOI=10.1007/978-1-4757-1647-4_13
Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=62055; DOI=10.1093/jnci/57.3.683
Biedler J.L., Spengler B.A.
A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture.
J. Natl. Cancer Inst. 57:683-695(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=922665; DOI=10.1002/1097-0142(197711)40:5<2256::AID-CNCR2820400536>3.0.CO;2-1
Brodeur G.M., Sekhon G., Goldstein M.N.
Chromosomal aberrations in human neuroblastomas.
Cancer 40:2256-2263(1977)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
Wright W.C., Daniels W.P., Fogh J.
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=7037175
Reynolds C.P., Reynolds D.A., Frenkel E.P., Smith R.G.
Selective toxicity of 6-hydroxydopamine and ascorbate for human neuroblastoma in vitro: a model for clearing marrow prior to autologous transplant.
Cancer Res. 42:1331-1336(1982)

PubMed=7139592; DOI=10.1016/0165-4608(82)90105-4
Gilbert F., Balaban G., Moorhead P.S., Bianchi D., Schlesinger H.R.
Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.
Cancer Genet. Cytogenet. 7:33-42(1982)

PubMed=6888561; DOI=10.1038/305245a0
Schwab M., Alitalo K., Klempnauer K.-H., Varmus H.E., Bishop J.M., Gilbert F., Brodeur G.M., Goldstein M.N., Trent J.
Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumour.
Nature 305:245-248(1983)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=2535691
Ciccarone V., Spengler B.A., Meyers M.B., Biedler J.L., Ross R.A.
Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.
Cancer Res. 49:219-225(1989)

PubMed=8221663
Komuro H., Hayashi Y., Kawamura M., Hayashi K., Kaneko Y., Kamoshita S., Hanada R., Yamamoto K., Hongo T., Yamada M., Tsuchida Y.
Mutations of the p53 gene are involved in Ewing's sarcomas but not in neuroblastomas.
Cancer Res. 53:5284-5288(1993)

DOI=10.1016/B978-0-12-333530-2.50006-X
Israel M.A., Thiele C.J.
Tumor cell lines of the peripheral nervous system.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.43-78; Academic Press; New York (1994)

PubMed=7838528
Cheng N.C., Van Roy N., Chan A., Beitsma M., Westerveld A., Speleman F., Versteeg R.
Deletion mapping in neuroblastoma cell lines suggests two distinct tumor suppressor genes in the 1p35-36 region, only one of which is associated with N-myc amplification.
Oncogene 10:291-297(1995)

PubMed=9516836; DOI=10.1016/S0959-8049(97)00319-5
Van Roy N., Laureys G., Van Gele M., Opdenakker G., Miura R., van der Drift P., Chan A., Versteeg R., Speleman F.
Analysis of 1;17 translocation breakpoints in neuroblastoma: implications for mapping of neuroblastoma genes.
Eur. J. Cancer 33:1974-1978(1997)

DOI=10.1007/0-306-46872-7_2
Thiele C.J.
Neuroblastoma.
(In) Human cell culture. Vol. 1. Cancer Cell Lines part 1; Masters J.R.W., Palsson B.O. (eds.); pp.21-53; Kluwer Academic Publishers; New York (1999)

PubMed=11550280; DOI=10.1002/gcc.1174
Van Roy N., Van Limbergen H., Vandesompele J., Van Gele M., Poppe B., Salwen H.R., Laureys G., Manoel N., De Paepe A., Speleman F.
Combined M-FISH and CGH analysis allows comprehensive description of genetic alterations in neuroblastoma cell lines.
Genes Chromosomes Cancer 32:126-135(2001)

PubMed=12210830; DOI=10.1002/jnr.10330
Yoshida S., Narita T., Taga T., Ohta S., Takeuchi Y.
Malignant rhabdoid tumor shows incomplete neural characteristics as revealed by expression of SNARE complex.
J. Neurosci. Res. 69:642-652(2002)

PubMed=12702577
Saito-Ohara F., Imoto I., Inoue J., Hosoi H., Nakagawara A., Sugimoto T., Inazawa J.
PPM1D is a potential target for 17q gain in neuroblastoma.
Cancer Res. 63:1876-1883(2003)

PubMed=16822308; DOI=10.1186/1471-2407-6-177
Dam V., Morgan B.T., Mazanek P., Hogarty M.D.
Mutations in PIK3CA are infrequent in neuroblastoma.
BMC Cancer 6:177-177(2006)

PubMed=22213050; DOI=10.1002/ijc.27415
Gawecka J.E., Geerts D., Koster J., Caliva M.J., Sulzmaier F.J., Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Int. J. Cancer 131:1556-1568(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

PubMed=28350380; DOI=10.1038/sdata.2017.33
Harenza J.L., Diamond M.A., Adams R.N., Song M.M., Davidson H.L., Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033-170033(2017)

Cross-references
Cell line collections ATCC; CCL-127
BCRC; 60014
BCRJ; 0377
CLS; 300148/p666_IMR-32
Coriell; GM03320
DSMZ; ACC-165
ECACC; 86041809
ICLC; HTL96021
IFO; IFO50283
IZSLER; BS TCL 29
JCRB; JCRB9050
KCB; KCB 2011100YJ
KCLB; 10127
RCB; RCB1895 - Discontinued
TKG; TKG 0207
Cell line databases/resources CLDB; cl2878
CLDB; cl2879
CLDB; cl2880
CLDB; cl2881
CLDB; cl2882
CLDB; cl2883
CLDB; cl2885
CLDB; cl2886
CLDB; cl2887
CLDB; cl2888
CCLE; IMR32_AUTONOMIC_GANGLIA
CCRID; 3111C0001CCC000282
CCRID; 3131C0001000700145
CGH-DB; 78-1
CGH-DB; 9095-4
LINCS_LDP; LCL-1965
Lonza; 971
Ontologies BTO; BTO:0000934
CLO; CLO_0006948
CLO; CLO_0016777
EFO; EFO_0001195
MCCL; MCC:0000242
Biological sample resources BioSample; SAMN03472412
BioSample; SAMN03473082
Chemistry resources ChEMBL-Cells; CHEMBL3307656
ChEMBL-Targets; CHEMBL614585
Gene expression databases GEO; GSM453632
GEO; GSM563362
GEO; GSM692859
GEO; GSM887162
GEO; GSM888234
GEO; GSM1366406
GEO; GSM1669934
GEO; GSM2371237
GEO; GSM2394364
Polymorphism and mutation databases Cosmic; 688067
Cosmic; 759892
Cosmic; 801746
Cosmic; 920239
Cosmic; 922658
Cosmic; 947702
Cosmic; 1019944
Cosmic; 1037352
Cosmic; 1099149
Cosmic; 1109111
Cosmic; 1237544
Cosmic; 1518066
Cosmic; 1526626
Cosmic; 2058113
Cosmic; 2131590
Cosmic; 2301580
Cosmic; 2393630
Cosmic; 2485944
Proteomic databases PRIDE; PXD000589