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Cellosaurus Jurkat E6.1 (CVCL_0367)

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Cell line name Jurkat E6.1
Synonyms JurkatE6-1; Jurkat E6-1; Jurkat, Clone E6-1; Jurkat Clone E6-1; Jurkat (clone E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1
Accession CVCL_0367
Resource Identification Initiative To cite this cell line use: Jurkat E6.1 (RRID:CVCL_0367)
Comments Group: Space-flown cell line (cellonaut).
Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Doubling time: 20.7 +- 2.2 hours (PubMed=10468210).
Omics: Cell surface proteome.
Omics: Deep phosphoproteome analysis.
Omics: Deep quantitative phosphoproteome analysis.
Omics: Deep RNAseq analysis.
Omics: Genome sequenced.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: SNP array analysis.
Anecdotal: Have been flown in space on shuttle flights STS-76, STS-80 and STS-95 to study apoptosis and cytoskeleton modifications (PubMed=9707173; PubMed=10832058; PubMed=11481229; PubMed=11669127).
Derived from sampling site: Peripheral blood.
Sequence variations Heterozygous for BAX p.Glu41Argfs*19 (c.121delG) (ClinVar=VCV000009512) (PubMed=29739316).
Heterozygous for BAX p.Glu41Glyfs*33 (c.121dupG) (ClinVar=VCV000009511) (PubMed=29739316).
Heterozygous for FBXW7 p.Arg505Cys (c.1513C>T) (ClinVar=VCV000069961) (from parent cell line).
Heterozygous for INPP5D p.Gln345Ter (c.1033C>T) (PubMed=29739316).
Heterozygous for INPP5D c.1097+1065_1097+1112del47 (PubMed=29739316).
Homozygous for MSH2 p.Arg711Ter (c.2131C>T) (ClinVar=VCV000090903) (PubMed=29739316).
Homozygous for MSH6 p.Phe1088Serfs*2 (c.3261delC) (ClinVar=VCV000089363) (from parent cell line).
Heterozygous for SYK p.Met34Hisfs*3 (c.98_99insG) (PubMed=7592872; PubMed=29739316).
Heterozygous for TP53 p.Arg196Ter (c.586C>T) (ClinVar=VCV000043589) (PubMed=29739316).
Disease Childhood T acute lymphoblastic leukemia (NCIt: C7953)
Precursor T-cell acute lymphoblastic leukemia (ORDO: Orphanet_99861)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0065 (Jurkat)
CVCL_6819 (10B10)CVCL_X694 (1G5)CVCL_A9P6 (Foxp3 reporter (Luc)-Jurkat)
CVCL_J124 (J-beta2.7)CVCL_0354 (J.CaM1.6)CVCL_R862 (J.CaM2)
CVCL_6410 (J.gamma1)CVCL_1316 (J.RT3-T3.5)CVCL_2530 (J45.01)
CVCL_JL96 (JKBulk)CVCL_LH47 (Jurkat 1F2)CVCL_WN96 (Jurkat E6.1 NF-kappaB::eGFP)
CVCL_DS01 (Jurkat J77)CVCL_DF51 (Jurkat NucLight Red)CVCL_JL98 (JXBulk)
CVCL_A9P4 (NF-kappaB reporter (Luc)-Jurkat)CVCL_6429 (P116)CVCL_D588 (T-REx-Jurkat)
Sex of cell Male
Age at sampling 14Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS; DOI=10.1101/2020.07.06.190801; ECACC; KCLB; PubMed=11416159; PubMed=25877200

CSF1PO10,11,12,13 (DOI=10.1101/2020.07.06.190801)
11,12 (ATCC; CCRID; CLS; ECACC; KCLB; PubMed=25877200)
D3S135815 (CCRID; KCLB)
15,17 (CLS; DOI=10.1101/2020.07.06.190801; PubMed=25877200)
D7S8208,10 (CLS; ECACC)
8,12 (ATCC; CCRID; DOI=10.1101/2020.07.06.190801; KCLB; PubMed=25877200)
D8S117912,13,14,15 (DOI=10.1101/2020.07.06.190801)
13,14 (CCRID; PubMed=11416159; PubMed=25877200)
D18S5112,13,20,21 (DOI=10.1101/2020.07.06.190801)
13,21 (CCRID; CLS; PubMed=11416159; PubMed=25877200)
D21S1130.2,31.2,33.2 (DOI=10.1101/2020.07.06.190801)
31.2,33.2 (CCRID; CLS; PubMed=11416159; PubMed=25877200)
Penta D11,13
Penta E10,12
vWA18 (ATCC; CCRID; CLS; ECACC; KCLB; PubMed=11416159; PubMed=25877200)
18,19 (DOI=10.1101/2020.07.06.190801)

Run an STR similarity search on this cell line
Web pages http://www.nibsc.org/Default.aspx?pageid=597&id=0027&theme=default

PubMed=8316623; DOI=10.2307/3578190
Evans H.H., Ricanati M., Horng M.-F., Jiang Q., Mencl J., Olive P.
DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.
Radiat. Res. 134:307-315(1993)

PubMed=7592872; DOI=10.1074/jbc.270.44.26533
Fargnoli J., Burkhardt A.L., Laverty M., Kut S.A., van Oers N.S.C., Weiss A., Bolen J.B.
Syk mutation in Jurkat E6-derived clones results in lack of p72syk expression.
J. Biol. Chem. 270:26533-26537(1995)

PubMed=9707173; DOI=10.1096/fasebj.12.11.1007
Lewis M.L., Reynolds J.L., Cubano L.A., Hatton J.P., Lawless B.D., Piepmeier E.H.
Spaceflight alters microtubules and increases apoptosis in human lymphocytes (Jurkat).
FASEB J. 12:1007-1018(1998)

PubMed=10468210; DOI=10.1016/S0891-5849(99)00074-X
Schoene N.W., Kamara K.S.
Population doubling time, phosphatase activity, and hydrogen peroxide generation in Jurkat cells.
Free Radic. Biol. Med. 27:364-369(1999)

PubMed=10832058; DOI=10.1016/S0531-5565(00)00090-5
Cubano L.A., Lewis M.L.
Fas/APO-1 protein is increased in spaceflown lymphocytes (Jurkat).
Exp. Gerontol. 35:389-400(2000)

PubMed=11481229; DOI=10.1096/fj.00-0820fje
Lewis M.L., Cubano L.A., Zhao B., Dinh H.-K., Pabalan J.G., Piepmeier E.H., Bowman P.D.
cDNA microarray reveals altered cytoskeletal gene expression in space-flown leukemic T lymphocytes (Jurkat).
FASEB J. 15:1783-1785(2001)

PubMed=11669127; DOI=10.1016/S0094-5765(01)00116-3
Schatten H., Lewis M.L., Chakrabarti A.
Spaceflight and clinorotation cause cytoskeleton and mitochondria changes and increases in apoptosis in cultured cells.
Acta Astronaut. 49:399-418(2001)

PubMed=15472075; DOI=10.1126/science.1102160
Weng A.P., Ferrando A.A., Lee W., Morris J.P. IV, Silverman L.B., Sanchez-Irizarry C., Blacklow S.C., Look A.T., Aster J.C.
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Science 306:269-271(2004)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=23933134; DOI=10.1016/j.jprot.2013.07.019
Storvold G.L., Landskron J., Strozynski M., Arntzen M.O., Koehler C.J., Kalland M.E., Tasken K., Thiede B.
Quantitative profiling of tyrosine phosphorylation revealed changes in the activity of the T cell receptor signaling pathway upon cisplatin-induced apoptosis.
J. Proteomics 91:344-357(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25894527; DOI=10.1371/journal.pone.0121314
Bausch-Fluck D., Hofmann A., Bock T., Frei A.P., Cerciello F., Jacobs A., Moest H., Omasits U., Gundry R.L., Yoon C., Schiess R., Schmidt A., Mirkowska P., Hartlova A., Van Eyk J.E., Bourquin J.-P., Aebersold R., Boheler K.R., Zandstra P., Wollscheid B.
A mass spectrometric-derived cell surface protein atlas.
PLoS ONE 10:E0121314-E0121314(2015)

PubMed=26546556; DOI=10.1016/j.jprot.2015.10.029
Nguyen T.D., Carrascal M., Vidal-Cortes O., Gallardo O., Casas V., Gay M., Phan V.C., Abian J.
The phosphoproteome of human Jurkat T cell clones upon costimulation with anti-CD3/anti-CD28 antibodies.
J. Proteomics 131:190-198(2016)

PubMed=28464451; DOI=10.1002/pmic.201600470
Tran T.T., Strozynski M., Thiede B.
Quantitative phosphoproteome analysis of cisplatin-induced apoptosis in Jurkat T cells.
Proteomics 17:1600470.1-1600470.11(2017)

PubMed=29739316; DOI=10.1186/s12864-018-4718-6
Gioia L., Siddique A., Head S.R., Salomon D.R., Su A.I.
A genome-wide survey of mutations in the Jurkat cell line.
BMC Genomics 19:334-334(2018)

Fernandez M.V., Hoffman H.K., Pezeshkian N., Tedbury P.R., van Engelenburg S.B., Freed E.O.
Elucidating the basis for permissivity of the MT-4 T-cell line to replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail.
bioRxiv 2020:07.06.190801-07.06.190801(2020)

Cell line collections ATCC; TIB-152
BCRC; 60424
BCRJ; 0125
CLS; 300223/p849_Jurkat_E61
ECACC; 88042803
KCB; KCB 94008YJ
KCLB; 40152
NCBI_Iran; C121
NIH-ARP; 177-450
Cell line databases/resources CLDB; cl2962
CCRID; 3111C0001CCC000075
CCRID; 3111C0001CCC000348
CCRID; 3111C0002000000049
CCRID; 3131C0001000700123
CCRID; 3142C0001000000080
Cell_Model_Passport; SIDM01244
TOKU-E; 2081
Ontologies BTO; BTO:0001948
CLO; CLO_0007045
MCCL; MCC:0000260
Biological sample resources BioSample; SAMN03472194
Chemistry resources PharmacoDB; Jurkat_Clone_E61_713_2019
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSE101101
GEO; GSM472928
GEO; GSM510511
GEO; GSM827151
GEO; GSM923424
GEO; GSM945267
GEO; GSM945268
Metabolomic databases MetaboLights; MTBLS789
Other Wikidata; Q54899132
Polymorphism and mutation databases Cosmic; 913419
Progenetix; CVCL_0367
Proteomic databases PRIDE; PRD000345
PRIDE; PXD000148
PRIDE; PXD000589
PRIDE; PXD002871
PRIDE; PXD004415
PRIDE; PXD010176
Entry history
Entry creation04-Apr-2012
Last entry update12-Jan-2021
Version number32