Cellosaurus LoVo (CVCL_0399)

Cell line name LoVo
Synonyms LOVO
Accession CVCL_0399
Resource Identification Initiative To cite this cell line use: LoVo (RRID:CVCL_0399)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
Doubling time: 37 hours (PubMed=1260746); 52 hours (PubMed=25984343); ~48 hours (DSMZ).
Microsatellite instability: Instable (MSI-high) (PubMed=9000147; PubMed=10674020; PubMed=12661003; PubMed=24042735; PubMed=25926053; Sanger).
Sequence variation: APC p.Arg1114Ter, p.Met1431fs*42 and p.Arg2816Gln (PubMed=24755471).
Sequence variation: KRAS p.Gly13Asp (PubMed=24755471).
Sequence variation: SMAD2 p.Ala292Val (PubMed=24755471).
Sequence variation: Has no TP53 mutation (PubMed=16418264).
Omics: Deep exome analysis.
Omics: Deep quantitative phosphoproteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: miRNA expression profiling.
Omics: N-glycan profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Colon adenocarcinoma (NCIt: C4349)
Derived from metastatic site: Left supraclavicular lymph node.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_5J42 (LoVo-6-luc-1); CVCL_5237 (LoVo-92); CVCL_4Y03 (LoVo-Luc#2); CVCL_V402 (LoVo-MEK-R); CVCL_6C49 (LoVo/DR5); CVCL_4V50 (LoVo/irinotecan); CVCL_4V75 (LoVo/L-OHP); CVCL_8375 (NCOL-1)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; CLS; Cosmic-CLP; DSMZ; ECACC; JCRB; KCLB; PubMed=11416159; PubMed=15771911; PubMed=25877200; RCB

Markers:
AmelogeninX,Y
CSF1PO11,13,14 (ATCC; ECACC; JCRB)
11,14 (Cosmic-CLP)
11,12,14 (DSMZ)
10,11,13,14 (AddexBio; CLS; PubMed=25877200)
10,14 (KCLB)
11,12,13 (RCB)
D13S3178,11
D16S5399,12 (AddexBio; ATCC; CLS; Cosmic-CLP; ECACC; JCRB; PubMed=15771911; PubMed=25877200; RCB)
9,11,12 (DSMZ)
D18S5113,18 (CLS; PubMed=15771911; PubMed=25877200)
13,17,18 (PubMed=11416159)
D19S43314,15 (PubMed=25877200)
15 (PubMed=15771911)
D21S1129,31.2,32.2 (CLS)
29,31.2 (PubMed=15771911; PubMed=25877200)
D2S133816,17,18 (PubMed=15771911)
17,18 (PubMed=25877200)
D3S135814,18 (KCLB; PubMed=25877200_584429)
14,16 (PubMed=15771911)
14,17 (PubMed=25877200_130903)
D5S81811,12,13 (AddexBio; ATCC; DSMZ; JCRB; RCB)
11,13 (CLS; Cosmic-CLP; ECACC; KCLB; PubMed=25877200)
D7S8209.3,10,11 (AddexBio; ATCC; DSMZ; PubMed=25877200)
10,11 (CLS; Cosmic-CLP; ECACC; JCRB; KCLB; RCB)
D8S11799,10 (PubMed=15771911)
10 (PubMed=25877200)
FGA18,20
Penta D9,10,14 (CLS)
10,14 (PubMed=25877200)
Penta E10,16
SE3319.2,20.2,25.2
TH019.3
TPOX8,9
vWA17,18 (AddexBio; ATCC; CLS; Cosmic-CLP; ECACC; JCRB; KCLB; PubMed=11416159; PubMed=25877200; RCB)
16,17,19 (DSMZ)
17,19 (PubMed=15771911)
Web pages http://www.pawefish.path.cam.ac.uk/ColonCellLineDescriptions/LoVo.html
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/l/cell-lines-detail-208.html
http://tcpaportal.org/mclp/
Publications

PubMed=1260746
Drewinko B., Romsdahl M.M., Yang L.Y., Ahearn M.J., Trujillo J.M.
Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line.
Cancer Res. 36:467-475(1976)

PubMed=7104989; DOI=10.1016/0165-4608(82)90076-0
Chen T.-R., Hay R.J., Macy M.L.
Karyotype consistency in human colorectal carcinoma cell lines established in vitro.
Cancer Genet. Cytogenet. 6:93-117(1982)

PubMed=9000147
Cottu P.H., Muzeau F., Estreicher A., Flejou J.-F., Iggo R., Thomas G., Hamelin R.
Inverse correlation between RER+ status and p53 mutation in colorectal cancer cell lines.
Oncogene 13:2727-2730(1996)

PubMed=9000572
Hoang J.-M., Cottu P.H., Thuille B., Salmon R.J., Thomas G., Hamelin R.
BAT-26, an indicator of the replication error phenotype in colorectal cancers and cell lines.
Cancer Res. 57:300-303(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9515795
Sparks A.B., Morin P.J., Vogelstein B., Kinzler K.W.
Mutational analysis of the APC/beta-catenin/Tcf pathway in colorectal cancer.
Cancer Res. 58:1130-1134(1998)

PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3
Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.
Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.
Eur. J. Cancer 35:1724-1729(1999)

PubMed=10612807; DOI=10.1002/(SICI)1098-2264(200002)27:2<183::AID-GCC10>3.0.CO;2-P
Ghadimi B.M., Sackett D.L., Difilippantonio M.J., Schrock E., Neumann T., Jauho A., Auer G., Ried T.
Centrosome amplification and instability occurs exclusively in aneuploid, but not in diploid colorectal cancer cell lines, and correlates with numerical chromosomal aberrations.
Genes Chromosomes Cancer 27:183-190(2000)

PubMed=10737795; DOI=10.1073/pnas.97.7.3352
Rowan A.J., Lamlum H., Ilyas M., Wheeler J., Straub J., Papadopoulou A., Bicknell D.C., Bodmer W.F., Tomlinson I.P.M.
APC mutations in sporadic colorectal tumors: a mutational 'hotspot' and interdependence of the 'two hits'.
Proc. Natl. Acad. Sci. U.S.A. 97:3352-3357(2000)

PubMed=11226274; DOI=10.1073/pnas.041603298
Abdel-Rahman W.M., Katsura K., Rens W., Gorman P.A., Sheer D., Bicknell D.C., Bodmer W.F., Arends M.J., Wyllie A.H., Edwards P.A.W.
Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement.
Proc. Natl. Acad. Sci. U.S.A. 98:2538-2543(2001)

PubMed=11314036; DOI=10.1038/sj.onc.1204211
Forgacs E., Wren J.D., Kamibayashi C., Kondo M., Xu X.L., Markowitz S.D., Tomlinson G.E., Muller C.Y., Gazdar A.F., Garner H.R., Minna J.D.
Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.
Oncogene 20:1005-1009(2001)

PubMed=11414198; DOI=10.1007/s004320000207
Lahm H., Andre S., Hoeflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E., Gabius H.J.
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=11526487; DOI=10.1038/sj.onc.1204611
Gayet J., Zhou X.-P., Duval A., Rolland S., Hoang J.-M., Cottu P.H., Hamelin R.
Extensive characterization of genetic alterations in a series of human colorectal cancer cell lines.
Oncogene 20:5025-5032(2001)

PubMed=12584437; DOI=10.1159/000068544
Melcher R., Koehler S., Steinlein C., Schmid M., Mueller C.R., Luehrs H., Menzel T., Scheppach W., Moerk H., Scheurlen M., Koehrle J., Al-Taie O.
Spectral karyotype analysis of colon cancer cell lines of the tumor suppressor and mutator pathway.
Cytogenet. Genome Res. 98:22-28(2002)

PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.-F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)

PubMed=15771911; DOI=10.1016/j.cancergencyto.2004.08.023
Melcher R., Maisch S., Koehler S., Bauer M., Steinlein C., Schmid M., Kudlich T., Schauber J., Luehrs H., Menzel T., Scheppach W.
SKY and genetic fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1.
Cancer Genet. Cytogenet. 158:84-87(2005)

PubMed=16418264; DOI=10.1073/pnas.0510146103
Liu Y., Bodmer W.F.
Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines.
Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006)

PubMed=16854228; DOI=10.1186/1476-4598-5-29
Bandres E., Cubedo E., Agirre X., Malumbres R., Zarate R., Ramirez N., Abajo A., Navarro A., Moreno I., Monzo M., Garcia-Foncillas J.
Identification by real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues.
Mol. Cancer 5:29-29(2006)

PubMed=19927377; DOI=10.1002/gcc.20730
Knutsen T., Padilla-Nash H.M., Wangsa D., Barenboim-Stapleton L., Camps J., McNeil N., Difilippantonio M.J., Ried T.
Definitive molecular cytogenetic characterization of 15 colorectal cancer cell lines.
Genes Chromosomes Cancer 49:204-223(2010)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=20606684; DOI=10.1038/sj.bjc.6605780
Bracht K., Nicholls A.M., Liu Y., Bodmer W.F.
5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.
Br. J. Cancer 103:340-346(2010)

PubMed=20831567; DOI=10.1111/j.1582-4934.2010.01170.x
Ma Y.-L., Zhang P., Wang F., Moyer M.P., Yang J.-J., Liu Z.-H., Peng J.-Y., Chen H.-Q., Zhou Y.-K., Liu W.-J., Qin H.-L.
Human embryonic stem cells and metastatic colorectal cancer cells shared the common endogenous human microRNA-26b.
J. Cell. Mol. Med. 15:1941-1954(2011)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24042735; DOI=10.1038/oncsis.2013.35
Ahmed D., Eide P.W., Eilertsen I.A., Danielsen S.A., Eknaes M., Hektoen M., Lind G.E., Lothe R.A.
Epigenetic and genetic features of 24 colon cancer cell lines.
Oncogenesis 2:E71-E71(2013)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35
Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Hill Meyers B., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002-7002(2015)

DOI=10.1101/092767
Roumeliotis T.I., Williams S.P., Goncalves E., Zamanzad G.F., Aben N., Michaut M., Schubert M., Wright J.C., Yang M., Alsinet C., Dienstmann R., Guinney J., Beltrao P., Brazma A., Stegle O., Adams D.J., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Choudhary J.S.
Genomic determinants of protein abundance variation in colorectal cancer cells.
bioRxiv 2016:092767-092767(2016)

PubMed=26537799; DOI=10.1074/mcp.M115.051235
Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J., Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E., Tollenaar R.A., Rombouts Y., Wuhrer M.
N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.
Mol. Cell. Proteomics 15:124-140(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections AddexBio; C0009011/380
ATCC; CCL-229
BCRC; 60148
BCRJ; 0332
CCLV; CCLV-RIE 1151
CLS; 300266/p487_LOVO
DSMZ; ACC-350
ECACC; 87060101
ICLC; HTL99028
IFO; IFO50067
IZSLER; BS TCL 205
JCRB; JCRB9083
KCLB; 10229
NCI-DTP; LOVO
RCB; RCB1639
TKG; TKG 0344 - Discontinued
Cell line databases/resources CLDB; cl3248
CLDB; cl3249
CLDB; cl3250
CLDB; cl4981
CCLE; LOVO_LARGE_INTESTINE
CCRID; 3111C0001CCC000164
CCRID; 3131C0001000700082
ColonAtlas; LOVO
Cosmic-CLP; 907790
GDSC; 907790
LINCS_LDP; LCL-1181
Lonza; 1021
SKY/M-FISH/CGH; 2878
Ontologies BTO; BTO:0000666
CLO; CLO_0007377
CLO; CLO_0007378
CLO; CLO_0050632
EFO; EFO_0006639
MCCL; MCC:0000293
Biological sample resources BioSample; SAMN03470964
BioSample; SAMN03471476
BioSample; SAMN03472104
BioSample; SAMN03472362
BioSample; SAMN03473360
ENCODE; ENCBS024NHD
ENCODE; ENCBS080BPN
Chemistry resources ChEMBL-Cells; CHEMBL3307691
ChEMBL-Targets; CHEMBL614721
Gene expression databases GEO; GSM206517
GEO; GSM274719
GEO; GSM274720
GEO; GSM274729
GEO; GSM513820
GEO; GSM514296
GEO; GSM741268
GEO; GSM784014
GEO; GSM887274
GEO; GSM888349
GEO; GSM1346882
GEO; GSM1374627
GEO; GSM1374628
GEO; GSM1374629
GEO; GSM1374630
GEO; GSM1448163
GEO; GSM1670055
Metabolomic databases MetaboLights; MTBLS227
Polymorphism and mutation databases Cosmic; 711256
Cosmic; 720330
Cosmic; 724842
Cosmic; 738931
Cosmic; 873702
Cosmic; 876724
Cosmic; 887220
Cosmic; 889534
Cosmic; 897741
Cosmic; 905003
Cosmic; 907790
Cosmic; 913886
Cosmic; 948858
Cosmic; 985997
Cosmic; 995396
Cosmic; 1043567
Cosmic; 1057754
Cosmic; 1066209
Cosmic; 1122327
Cosmic; 1132566
Cosmic; 1132691
Cosmic; 1184084
Cosmic; 1184329
Cosmic; 1187308
Cosmic; 1223145
Cosmic; 1312303
Cosmic; 1466817
Cosmic; 1479597
Cosmic; 1482522
Cosmic; 1486133
Cosmic; 1524331
Cosmic; 1552182
Cosmic; 1571770
Cosmic; 1676729
Cosmic; 1609489
Cosmic; 1708412
Cosmic; 1803948
Cosmic; 1927244
Cosmic; 1945867
Cosmic; 1995488
Cosmic; 2267319
Cosmic; 2301996