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Cellosaurus MC3T3-E1 (CVCL_0409)

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Cell line name MC3T3-E1
Synonyms Mc3T3-E1; MC3T3E1; MC-3T3-E1; MC 3T3-E1
Accession CVCL_0409
Resource Identification Initiative To cite this cell line use: MC3T3-E1 (RRID:CVCL_0409)
Comments Group: Space-flown cell line (cellonaut).
Doubling time: ~1-2 days (DSMZ).
Omics: SNP array analysis.
Anecdotal: Have been flown in space on shuttle flights STS-56, STS-76, STS-81 and STS-84 to study growth and differentiation in microgravity (PubMed=8612673; PubMed=12638602).
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Parent: CVCL_0D74 (MC3T3)
Children:
CVCL_VT93 (MC3T3-E1 Subclone 11)CVCL_5437 (MC3T3-E1 Subclone 14)CVCL_VT94 (MC3T3-E1 Subclone 17)
CVCL_VT95 (MC3T3-E1 Subclone 20)CVCL_5438 (MC3T3-E1 Subclone 24)CVCL_VT96 (MC3T3-E1 Subclone 26)
CVCL_5439 (MC3T3-E1 Subclone 30)CVCL_VT97 (MC3T3-E1 Subclone 35)CVCL_5440 (MC3T3-E1 Subclone 4)
CVCL_VT98 (MC3T3-E1 Subclone 8)
Age at sampling <1D
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/MC3T3
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-393.html
http://www.cellresource.cn/fdetail.aspx?id=12
Publications

DOI=10.2330/joralbiosci1965.23.899
Kodama H.-A., Amagai Y., Sudo H., Kasai S., Yamamoto S.
Establishment of a clonal osteogenic cell line from newborn mouse calvaria.
Jpn. J. Oral Biol. 23:899-901(1981)

PubMed=6826647; DOI=10.1083/jcb.96.1.191
Sudo H., Kodama H.-A., Amagai Y., Yamamoto S., Kasai S.
In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria.
J. Cell Biol. 96:191-198(1983)

PubMed=8612673; DOI=10.1006/excr.1996.0116
Hughes-Fulford M., Lewis M.L.
Effects of microgravity on osteoblast growth activation.
Exp. Cell Res. 224:103-109(1996)

PubMed=10352097; DOI=10.1359/jbmr.1999.14.6.893
Wang D., Christensen K., Chawla K., Xiao G., Krebsbach P.H., Franceschi R.T.
Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential.
J. Bone Miner. Res. 14:893-903(1999)

PubMed=12638602
Hughes-Fulford M.
Changes in gene expression and signal transduction in microgravity.
J. Gravit. Physiol. 8:P1-P4(2001)

DOI=10.4051/ibc.2010.2.4.0014
Yoshino K., Saijo K., Noro C., Nakamura Y.
Development of a simple method to determine the mouse strain from which cultured cell lines originated.
Interdisciplinary Bio Central 2:1-9(2010)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, de Villena F.P.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847-847(2014)

Cross-references
Cell line collections ATCC; CRL-2953 - Discontinued
CCTCC; GDC0188
DSMZ; ACC-210
ECACC; 99072810
KCB; KCB 200786YJ
RCB; RCB1126
Cell line databases/resources CLDB; cl40
CCRID; 3111C0001CCC000012
FCS-free; 185-5-353-1-3-3
Lonza; 521
Ontologies BTO; BTO:0001957
CLO; CLO_0007582
CLO; CLO_0050208
MCCL; MCC:0000303
Other Wikidata; Q54904201
Entry history
Entry creation04-Apr-2012
Last entry updated05-Jul-2019
Version number18