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Cellosaurus MDBK (CVCL_0421)

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Cell line name MDBK
Synonyms MDBK (NBL-1); NBL-1; Madin-Darby Bovine Kidney; Madin Darby Bovine Kidney
Accession CVCL_0421
Resource Identification Initiative To cite this cell line use: MDBK (RRID:CVCL_0421)
Comments Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Doubling time: ~35 hours (DSMZ).
Derived from sampling site: Kidney.
Species of origin Bos taurus (Bovine) (NCBI Taxonomy: 9913)
Hierarchy Children:
CVCL_DD22 (BHH2C)CVCL_DD23 (BHH3)CVCL_DD24 (BHH8)
CVCL_DD19 (CRIB-1)CVCL_DD25 (M2B)CVCL_DD20 (MDBK clone 955-1)
CVCL_DD21 (MDBK clone 958-9)CVCL_VD89 (MDBK DeltaGPI)CVCL_RT35 (MDBK-221)
CVCL_4541 (MDBK-Bu100)CVCL_E073 (MDBK-NST)CVCL_0I74 (MDBK-PES)
CVCL_RX00 (MDBK-t2)CVCL_3021 (MDBK.P3)CVCL_IZ74 (MDBK/441)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages http://www.cells-talk.com/index.php/page/copelibrary?key=MDBK
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-133.html
https://www.microscopyu.com/gallery-images/madin-darby-bovine-kidney-epithelial-cells-mdbk-line
http://www.cellresource.cn/fdetail.aspx?id=288
http://www.cellresource.cn/fdetail.aspx?id=448
http://www.cellresource.cn/fdetail.aspx?id=512
http://www.cellresource.cn/fdetail.aspx?id=1307
Publications

PubMed=13567776; DOI=10.3181/00379727-98-24111
Madin S.H., Darby N.B. Jr.
Established kidney cell lines of normal adult bovine and ovine origin.
Proc. Soc. Exp. Biol. Med. 98:574-576(1958)

PubMed=14207851; DOI=10.1093/jnci/33.2.347
Nelson-Rees W.A., Kniazeff A.J., Darby N.B. Jr.
Preservation of bulk chromatin with decrease in number of chromosomes in cells of an established bovine kidney line.
J. Natl. Cancer Inst. 33:347-361(1964)

PubMed=5988125; DOI=10.1007/BF00326444
Nelson-Rees W.A., Darby N.B. Jr., Siegel S.T.
Clonal derivatives of a bovine kidney cell line (MDBK) and continuing preservation of total chromosomal material.
Chromosoma 18:70-78(1966)

PubMed=5587551; DOI=10.1159/000129961
Nelson-Rees W.A., Kniazeff A.J., Darby N.B. Jr.
Debut and accumulation of centric fusion products: an index to age of certain cell lines.
Cytogenetics 6:436-450(1967)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=24973239; DOI=10.1099/vir.0.065995-0
Richter M., Reimann I., Schirrmeier H., Kirkland P.D., Beer M.
The viral envelope is not sufficient to transfer the unique broad cell tropism of Bungowannah virus to a related pestivirus.
J. Gen. Virol. 95:2216-2222(2014)

Cross-references
Cell line collections ATCC; CCL-22
ATCC; CRL-6071 - Discontinued
BCRC; 60126
BCRJ; 0167
CCLV; CCLV-RIE 0084
CCLV; CCLV-RIE 0261
CCTCC; GDC0108
CCTCC; GDC0290
CLS; 600396/p848_MDBK_(NBL-1)
DSMZ; ACC-174
ECACC; 90050801
IZSLER; BS CL 63
JCRB; IFO50014
JCRB; JCRB9028
KCB; KCB 90031YJ
KCLB; 10022
NCBI_Iran; C500
RCB; RCB0180
Cell line databases/resources CLDB; cl3412
CLDB; cl3413
CLDB; cl3414
CLDB; cl3415
CLDB; cl3416
CCRID; 3111C0001CCC000288
CCRID; 3111C0002000000076
CCRID; 3131C0001000400007
CCRID; 3153C0001000000033
FCS-free; 40-4-47-1-8-6
Lonza; 925
Ontologies BTO; BTO:0000836
CLO; CLO_0007642
CLO; CLO_0007643
CLO; CLO_0050865
MCCL; MCC:0000318
Other Wikidata; Q54904675
Entry history
Entry creation04-Apr-2012
Last entry update02-Jul-2020
Version number27