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Cellosaurus MDCK (CVCL_0422)

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Cell line name MDCK
Synonyms MDCK (NBL-2); NBL-2; Madin-Darby Canine Kidney; Madin Darby Canine Kidney
Accession CVCL_0422
Resource Identification Initiative To cite this cell line use: MDCK (RRID:CVCL_0422)
Comments Group: Vaccine production cell line.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Caution: There seems to be two distinct cell lines which were assigned NCBI_Iran catalog number C426.
Species of origin Canis lupus familiaris (Dog) (Canis familiaris) (NCBI Taxonomy: 9615)
Breed/subspecies: Cocker Spaniel.
Hierarchy Children:
CVCL_4164 (DoCl1 (S+L-))CVCL_WL43 (hCK)CVCL_GY95 (Luc9.1)
CVCL_2A36 (MDCK 33016)CVCL_DF69 (MDCK 9B9-1B1)CVCL_DF70 (MDCK 9B9-1E4)
CVCL_AP67 (MDCK Sky1023)CVCL_AP68 (MDCK Sky10234)CVCL_AP69 (MDCK Sky3851)
CVCL_V359 (MDCK Tet-Off)CVCL_WL59 (MDCK-6A8)CVCL_K177 (MDCK-AD)
CVCL_AP66 (MDCK-B-702)CVCL_AP70 (MDCK-C11)CVCL_0423 (MDCK-C7)
CVCL_DF31 (MDCK-chAbcb1)CVCL_AQ31 (MDCK-E [Korea])CVCL_Y089 (MDCK-E [Taiwan])
CVCL_AP75 (MDCK-F)CVCL_0592 (MDCK-I)CVCL_0424 (MDCK-II)
CVCL_IZ19 (MDCK-P-gp)CVCL_JX35 (MDCK-Protein Free)CVCL_IQ72 (MDCK-S)
CVCL_IQ73 (MDCK-SF101)CVCL_IQ74 (MDCK-SF102)CVCL_IQ75 (MDCK-SF103)
CVCL_WL74 (MDCK-SFS)CVCL_Z936 (MDCK-SIAT1)CVCL_WC98 (MDCK/IgR)
CVCL_B033 (MDCK.1)CVCL_B034 (MDCK.2)CVCL_DE56 (MDCK.5F1)
CVCL_2586 (MDCK.P3)CVCL_WL70 (MDCK.SUS1)CVCL_K241 (MDCK/60)
CVCL_WL76 (ssf-MDCK)CVCL_6452 (Super Dome)CVCL_6453 (Super Tube)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages https://en.wikipedia.org/wiki/Madin-Darby_Canine_Kidney_Cells
https://www.atcc.org/support/faqs/5889f/Morphology%20of%20ATCC%20CCL34%20MDCK-444.aspx
https://www.phe-culturecollections.org.uk/media/137096/mcdk-cell-line-profile.pdf
http://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/mdck/mdckcells.html
http://en.vircell.com/products/mdck-cell-line/
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-89.html
http://www.cellresource.cn/fdetail.aspx?id=78
http://www.cellresource.cn/fdetail.aspx?id=417
http://www.cellresource.cn/fdetail.aspx?id=518
http://www.cellresource.cn/fdetail.aspx?id=527
http://www.cellresource.cn/fdetail.aspx?id=780
http://www.cellresource.cn/fdetail.aspx?id=915
Publications

PubMed=13901412; DOI=10.1126/science.138.3536.42
Green I.J.
Serial propagation of influenza B (Lee) virus in a transmissible line of canine kidney cells.
Science 138:42-43(1962)

PubMed=5918973; DOI=10.3181/00379727-122-31293
Gaush C.R., Hard W.L., Smith T.F.
Characterization of an established line of canine kidney cells (MDCK).
Proc. Soc. Exp. Biol. Med. 122:931-935(1966)

PubMed=222773; DOI=10.1083/jcb.81.3.635
Rindler M.J., Chuman L.M., Shaffer L., Saier M.H. Jr.
Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).
J. Cell Biol. 81:635-648(1979)

PubMed=12667817; DOI=10.1016/S0042-6822(02)00064-8
Romanova J., Katinger D., Ferko B., Voglauer R., Mochalova L., Bovin N., Lim W., Katinger H., Egorov A.
Distinct host range of influenza H3N2 virus isolates in Vero and MDCK cells is determined by cell specific glycosylation pattern.
Virology 307:90-97(2003)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=21819694
Omeir R.L., Teferedegne B., Foseh G.S., Beren J.J., Snoy P.J., Brinster L.R., Cook J.L., Peden K., Lewis A.M. Jr.
Heterogeneity of the tumorigenic phenotype expressed by Madin-Darby canine kidney cells.
Comp. Med. 61:243-250(2011)

PubMed=21982418; DOI=10.1186/1471-2121-12-43
Dukes J.D., Whitley P., Chalmers A.D.
The MDCK variety pack: choosing the right strain.
BMC Cell Biol. 12:43-43(2011)

PubMed=24058646; DOI=10.1371/journal.pone.0075014
Lugovtsev V.Y., Melnyk D., Weir J.P.
Heterogeneity of the MDCK cell line and its applicability for influenza virus research.
PLoS ONE 8:E75014-E75014(2013)

PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

Cross-references
Cell line collections AddexBio; P0014003/4928
ATCC; CCL-34
ATCC; CRL-6253 - Discontinued
BCRC; 60004
BCRJ; 0168
BEI_Resources; NR-2628
CCLV; CCLV-RIE 0083
CCTCC; GDC0012
CCTCC; GDC0401
CLS; 602280/p823_MDCK_(NBL-2)
ECACC; 84121903
ECACC; 85011435
IBRC; C10556
IZSLER; BS CL 64
JCRB; IFO50071
JCRB; JCRB9029
KCB; KCB 2006105YJ
KCLB; 10034
NCBI_Iran; C426
RCB; RCB0995
Cell line databases/resources CLDB; cl3417
CLDB; cl3418
CLDB; cl3419
CLDB; cl3420
CLDB; cl3421
CLDB; cl3422
CLDB; cl3423
CLDB; cl3424
CLDB; cl3425
CCRID; 3111C0001CCC000078
CCRID; 3111C0002000000045
CCRID; 3131C0001000400014
CCRID; 3131C0001000400024
CCRID; 3142C0001000000012
CCRID; 3142C0001000000147
FCS-free; 41-14-47-1-8-9
FCS-free; 41-14-321-2-3-3
FCS-free; 41-14-427-1-4-3
FCS-free; 41-14-585-1-18-2
FCS-free; 41-14-587-1-18-2
FCS-free; 41-14-588-2-18-2
Lonza; 153
TOKU-E; 2403
TOKU-E; 3636
Ontologies BTO; BTO:0000837
CLO; CLO_0007646
CLO; CLO_0007647
CLO; CLO_0050861
MCCL; MCC:0000319
MeSH; D061985
Chemistry resources ChEMBL-Cells; CHEMBL3308370
ChEMBL-Targets; CHEMBL614068
Other Wikidata; Q28335011
Entry history
Entry creation04-Apr-2012
Last entry updated05-Jul-2019
Version number26