Cellosaurus MG-63 (CVCL_0426)

Cell line name MG-63
Synonyms M-G63; MG63
Accession CVCL_0426
Resource Identification Initiative To cite this cell line use: MG-63 (RRID:CVCL_0426)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: MD Anderson Cell Lines Project.
Doubling time: 38 hours (PubMed=218153); 28.3 hours (PubMed=19363654).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Metabolome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Misspelling: MGG3; In figure 4 of PubMed=2823272.
Disease Osteosarcoma (NCIt: C9145)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_LM95 (3AB-OS)CVCL_IR36 (MG-63.3A)CVCL_9U34 (MG63/DXR1000)
CVCL_R705 (MG63.2)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): ATCC; CLS; Cosmic-CLP; ECACC; JCRB; KCLB; PubMed=25877200; RCB; TKG

Markers:
AmelogeninX,Y
CSF1PO10,12
D13S31711
D16S53911 (ATCC; CLS; Cosmic-CLP; ECACC; PubMed=25877200; RCB; TKG)
11,12 (JCRB)
D18S5113 (CLS)
16 (PubMed=25877200)
D21S1128,29 (CLS)
30 (PubMed=25877200)
D3S135815,17 (CLS)
15 (KCLB; PubMed=25877200)
D5S81811,12
D7S82010
D8S117911,15 (CLS)
13 (PubMed=25877200)
FGA20,21 (CLS)
21,25 (KCLB; PubMed=25877200)
Penta D11,13 (CLS)
9,13 (PubMed=25877200)
Penta E12 (CLS)
11,12 (PubMed=25877200)
TH019.3
TPOX8,11
vWA16,19
Web pages http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-91.html
http://tcpaportal.org/mclp/
Publications

PubMed=883813
Billiau A., Edy V.G., Heremans H., Van Damme J., Desmyter J., Georgiades J.A., De Somer P.
Human interferon: mass production in a newly established cell line, MG-63.
Antimicrob. Agents Chemother. 12:11-15(1977)

PubMed=218153; DOI=10.1159/000225298
Heremans H., Billiau A., Cassiman J.J., Mulier J.C., de Somer P.
In vitro cultivation of human tumor tissues. II. Morphological and virological characterization of three cell lines.
Oncology 35:246-252(1978)

PubMed=2823272; DOI=10.1073/pnas.84.21.7716
Masuda H., Miller C., Koeffler H.P., Battifora H., Cline M.J.
Rearrangement of the p53 gene in human osteogenic sarcomas.
Proc. Natl. Acad. Sci. U.S.A. 84:7716-7719(1987)

PubMed=7873286; DOI=10.1016/8756-3282(94)90305-0
Clover J., Gowen M.
Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype?
Bone 15:585-591(1994)

PubMed=8617485; DOI=10.1016/S0046-8177(96)90115-X
Scotlandi K., Serra M., Manara M.C., Benini S., Sarti M., Maurici D., Lollini P.-L., Picci P., Bertoni F., Baldini N.
Immunostaining of the p30/32MIC2 antigen and molecular detection of EWS rearrangements for the diagnosis of Ewing's sarcoma and peripheral neuroectodermal tumor.
Hum. Pathol. 27:408-416(1996)

PubMed=12645653; DOI=10.1016/S0165-4608(02)00685-4
Ozaki T., Neumann T., Wai D.H., Schaefer K.-L., van Valen F., Lindner N., Scheel C., Bocker W., Winkelmann W., Dockhorn-Dworniczak B., Horst J., Poremba C.
Chromosomal alterations in osteosarcoma cell lines revealed by comparative genomic hybridization and multicolor karyotyping.
Cancer Genet. Cytogenet. 140:145-152(2003)

PubMed=15736406
Pautke C., Schieker M., Tischer T., Kolk A., Neth P., Mutschler W., Milz S.
Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts.
Anticancer Res. 24:3743-3748(2004)

PubMed=19160414; DOI=10.1002/jcp.21667
Di Fiore R., Santulli A., Ferrante R.D., Giuliano M., De Blasio A., Messina C., Pirozzi G., Tirino V., Tesoriere G., Vento R.
Identification and expansion of human osteosarcoma-cancer-stem cells by long-term 3-aminobenzamide treatment.
J. Cell. Physiol. 219:301-313(2009)

PubMed=19363654; DOI=10.1007/s10585-009-9259-6
Su Y.-X., Luo X.-J., He B.-C., Wang Y., Chen L., Zuo G.-W., Liu B., Bi Y., Huang J.-Y., Zhu G.-H., He Y., Kang Q., Luo J.-Y., Shen J.-K., Chen J., Jin X.-Q., Haydon R.C., He T.-C., Luu H.-H.
Establishment and characterization of a new highly metastatic human osteosarcoma cell line.
Clin. Exp. Metastasis 26:599-610(2009)

PubMed=19787792; DOI=10.1002/gcc.20717
Ottaviano L., Schaefer K.-L., Gajewski M., Huckenbeck W., Baldus S., Rogel U., Mackintosh C., de Alava E., Myklebost O., Kresse S.H., Meza-Zepeda L.A., Serra M., Cleton-Jansen A.-M., Hogendoorn P.C.W., Buerger H., Aigner T., Gabbert H.E., Poremba C.
Molecular characterization of commonly used cell lines for bone tumor research: a trans-European EuroBoNet effort.
Genes Chromosomes Cancer 49:40-51(2010)

PubMed=21519327; DOI=10.1038/labinvest.2011.72
Mohseny A.B., Machado I., Cai Y., Schaefer K.-L., Serra M., Hogendoorn P.C.W., Llombart-Bosch A., Cleton-Jansen A.-M.
Functional characterization of osteosarcoma cell lines provides representative models to study the human disease.
Lab. Invest. 91:1195-1205(2011)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23129384; DOI=10.1002/jcp.24272
Di Fiore R., Fanale D., Drago-Ferrante R., Chiaradonna F., Giuliano M., De Blasio A., Amodeo V., Corsini L.R., Bazan V., Tesoriere G., Vento R., Russo A.
Genetic and molecular characterization of the human osteosarcoma 3AB-OS cancer stem cell line: a possible model for studying osteosarcoma origin and stemness.
J. Cell. Physiol. 228:1189-1201(2013)

PubMed=25382592; DOI=10.1021/pr500907d
Lamego I., Duarte I.F., Marques M.P.M., Gil A.M.
Metabolic markers of MG-63 osteosarcoma cell line response to doxorubicin and methotrexate treatment: comparison to cisplatin.
J. Proteome Res. 13:6033-6045(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections ATCC; CRL-1427
BCRC; 60279
BCRJ; 0173
CLS; 300441/p2938_MG-63
ECACC; 86051601
ICLC; HTL99003
IFO; IFO50108
IZSLER; BS TCL 40
KCB; KCB 200528YJ
KCLB; 21427
NCBI_Iran; C555
RCB; RCB1890
TKG; TKG 0294
Cell line databases/resources CLDB; cl3462
CLDB; cl3463
CLDB; cl3464
CLDB; cl3465
CLDB; cl4934
CCLE; MG63_BONE
CCRID; 3111C0001CCC000080
CCRID; 3131C0001000700124
CCRID; 3142C0001000000073
Cosmic-CLP; 908131
GDSC; 908131
IGRhCellID; MG63
LINCS_LDP; LCL-1428
Lonza; 872
TOKU-E; 2429
Ontologies BTO; BTO:0001596
CLO; CLO_0007699
CLO; CLO_0007701
CLO; CLO_0050808
EFO; EFO_0002234
MCCL; MCC:0000323
Biological sample resources BioSample; SAMN03471910
BioSample; SAMN03472771
ENCODE; ENCBS091SUW
ENCODE; ENCBS462PMF
Chemistry resources ChEMBL-Cells; CHEMBL3308498
ChEMBL-Targets; CHEMBL614347
Gene expression databases GEO; GSM170251
GEO; GSM320828
GEO; GSM827317
GEO; GSM879217
GEO; GSM887314
GEO; GSM888390
GEO; GSM1670103
Polymorphism and mutation databases Cosmic; 755310
Cosmic; 908131
Cosmic; 931036
Cosmic; 931913
Cosmic; 1044085
Cosmic; 1070844
Cosmic; 1074394
Cosmic; 1082504
Cosmic; 1188475
Cosmic; 1529903