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Cellosaurus MKN45 (CVCL_0434)

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Cell line name MKN45
Synonyms MKN-45; MKN 45
Accession CVCL_0434
Resource Identification Initiative To cite this cell line use: MKN45 (RRID:CVCL_0434)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: JFCR39 cancer cell line panel.
Part of: JFCR45 cancer cell line panel.
Part of: MD Anderson Cell Lines Project.
Doubling time: 30-33 hours (PubMed=3962675); 16 hours (PubMed=2105279); ~60 hours (DSMZ).
Microsatellite instability: Stable (MSS) (PubMed=23671654; Sanger).
Omics: Deep exome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Caution: Was indicated not to have a TP53 mutation in PubMed=1370612 and PubMed=15900046.
Misspelling: MKN46; In Cosmic 968349.
Misspelling: MNK-45; Occasionally.
Misspelling: MNK45; Occasionally.
Misspelling: NKM-45; Occasionally.
Misspelling: NKM45; Occasionally.
Sequence variations Heterozygous for TP53 p.Arg110Cys (c.328C>T) (CCLE; Cosmic-CLP).
HLA typing Source: PubMed=25960936
Class I
HLA-AA*24:02,24:02
HLA-BB*52:01
HLA-CC*12:02,12:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.54
Native American0
East Asian, North85.37
East Asian, South9.42
South Asian0
European, North0
European, South3.67
Disease Gastric adenocarcinoma (NCIt: C4004)
Derived from metastatic site: Liver.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_7668 (GTL-16)CVCL_XE84 (MKN45-Cas9-526)CVCL_UW57 (MKN45-EGFP)
CVCL_J262 (MKN45-Luc)CVCL_QY39 (MKN45/F2R)CVCL_9645 (MKN45/TP)
CVCL_RM02 (MKN45G)CVCL_Y476 (MKN45P)CVCL_Z941 (MKN45R0.05)
CVCL_Z942 (MKN45R0.08)
Sex of cell Female
Age at sampling 62Y
Category Cancer cell line
STR profile Source(s): CCRID; Cosmic-CLP; DSMZ; JCRB; KCLB; PubMed=25877200; RCB

Markers:
AmelogeninX
CSF1PO12
D3S135815,16
D5S81810,11
D7S82010,11
D8S117913,17
D13S3178,11
D16S53910
D18S5116
D19S43314,16.2
D21S1131
FGA19 (KCLB)
19,24 (CCRID; PubMed=25877200)
Penta D10
Penta E10
TH017
TPOX8
vWA19

Run an STR similarity search on this cell line
Web pages http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-496.html
http://tcpaportal.org/mclp/
http://www.cellresource.cn/fdetail.aspx?id=229
Publications

PubMed=6862145; DOI=10.20772/cancersci1959.74.2_240
Naito S., Inoue S., Kinjo M., Tanaka K.
Thromboplastic and fibrinolytic activities of cultured human gastric cancer cell lines.
Gann 74:240-247(1983)

PubMed=3986962; DOI=10.1093/carcin/6.4.549
Watatani M., Ikenaga M., Hatanaka T., Kinuta M., Takai S.-I., Mori T., Kondo S.
Analysis of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced DNA damage in tumor cell strains from Japanese patients and demonstration of MNNG hypersensitivity of Mer xenografts in athymic nude mice.
Carcinogenesis 6:549-553(1985)

PubMed=3962675; DOI=10.1111/j.1440-1827.1986.tb01461.x
Motoyama T., Hojo H., Watanabe H.
Comparison of seven cell lines derived from human gastric carcinomas.
Acta Pathol. Jpn. 36:65-83(1986)

PubMed=2105279; DOI=10.1002/ijc.2910450117
Watson S.A., Durrant L.G., Morris D.L.
The effect of the E2 prostaglandin enprostil, and the somatostatin analogue SMS 201 995, on the growth of a human gastric cell line, MKN45G.
Int. J. Cancer 45:90-94(1990)

PubMed=1778766; DOI=10.1111/j.1349-7006.1991.tb01816.x
Takeshima E., Hamaguchi M., Watanabe T., Akiyama S., Kataoka M., Ohnishi Y., Xiao H.Y., Nagai Y., Takagi H.
Aberrant elevation of tyrosine-specific phosphorylation in human gastric cancer cells.
Jpn. J. Cancer Res. 82:1428-1435(1991)

PubMed=1846553; DOI=10.1038/bjc.1991.14
Durrant L.G., Watson S.A., Hall A., Morris D.L.
Co-stimulation of gastrointestinal tumour cell growth by gastrin, transforming growth factor alpha and insulin like growth factor-I.
Br. J. Cancer 63:67-70(1991)

PubMed=1933850
Yamada Y., Yoshida T., Hayashi K., Sekiya T., Yokota J., Hirohashi S., Nakatani K., Nakano H., Sugimura T., Terada M.
p53 gene mutations in gastric cancer metastases and in gastric cancer cell lines derived from metastases.
Cancer Res. 51:5800-5805(1991)

PubMed=2061947; DOI=10.1093/jnci/83.12.866
Watson S.A., Durrant L.G., Wencyk P.M., Watson A.L., Morris D.L.
Intracellular gastrin in human gastrointestinal tumor cells.
J. Natl. Cancer Inst. 83:866-871(1991)

PubMed=1370612; DOI=10.1016/S0006-291X(05)80133-0
Matozaki T., Sakamoto C., Matsuda K., Suzuki T., Konda Y., Nakano O., Wada K., Uchida T., Nishisaki H., Nagao M., Kasuga M.
Missense mutations and a deletion of the p53 gene in human gastric cancer.
Biochem. Biophys. Res. Commun. 182:215-223(1992)

PubMed=1486568; DOI=10.1016/0165-4608(92)90350-H
Rege-Cambrin G., Scaravaglio P., Carozzi F., Giordano S., Ponzetto C., Comoglio P.M., Saglio G.
Karyotypic analysis of gastric carcinoma cell lines carrying an amplified c-met oncogene.
Cancer Genet. Cytogenet. 64:170-173(1992)

DOI=10.1016/B978-0-12-333530-2.50014-9
Sekiguchi M., Suzuki T.
Gastric tumor cell lines.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.287-316; Academic Press; New York (1994)

PubMed=9023415; DOI=10.1006/cimm.1996.1062
Seki N., Hoshino T., Kikuchi M., Hayashi A., Itoh K.
HLA-A locus-restricted and tumor-specific CTLs in tumor-infiltrating lymphocytes of patients with non-small cell lung cancer.
Cell. Immunol. 175:101-110(1997)

PubMed=9247707; DOI=10.1080/15216549700202901
Hatakeyama S., Gao Y.-H., Ohara-Nemoto Y., Kataoka H., Satoh M.
Expression of bone morphogenetic proteins of human neoplastic epithelial cells.
Biochem. Mol. Biol. Int. 42:497-505(1997)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=9665481; DOI=10.1016/S0002-9440(10)65561-7
Paciucci R., Vila M.R., Adell T., Diaz V.M., Tora M., Nakamura T., Real F.X.
Activation of the urokinase plasminogen activator/urokinase plasminogen activator receptor system and redistribution of E-cadherin are associated with hepatocyte growth factor-induced motility of pancreas tumor cells overexpressing Met.
Am. J. Pathol. 153:201-212(1998)

PubMed=11107048; DOI=10.1046/j.1440-1827.2000.01117.x
Yokozaki H.
Molecular characteristics of eight gastric cancer cell lines established in Japan.
Pathol. Int. 50:767-777(2000)

PubMed=15723654; DOI=10.1111/j.1349-7006.2005.00016.x
Takada H., Imoto I., Tsuda H., Sonoda I., Ichikura T., Mochizuki H., Okanoue T., Inazawa J.
Screening of DNA copy-number aberrations in gastric cancer cell lines by array-based comparative genomic hybridization.
Cancer Sci. 96:100-110(2005)

PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Nakatsu N., Yoshida Y., Yamazaki K., Nakamura T., Dan S., Fukui Y., Yamori T.
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)

PubMed=15900046; DOI=10.1093/jnci/dji133
Mashima T., Oh-hara T., Sato S., Mochizuki M., Sugimoto Y., Yamazaki K., Hamada J., Tada M., Moriuchi T., Ishikawa Y., Kato Y., Tomoda H., Yamori T., Tsuruo T.
p53-defective tumors with a functional apoptosome-mediated pathway: a new therapeutic target.
J. Natl. Cancer Inst. 97:765-777(2005)

PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
Murai Y., Hayashi S., Takahashi H., Tsuneyama K., Takano Y.
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
Pathol. Res. Pract. 201:109-115(2005)

PubMed=18804159; DOI=10.1016/j.ygeno.2008.08.002
Jung J.-J., Jeung H.-C., Chung H.C., Lee J.O., Kim T.S., Kim Y.T., Noh S.H., Rha S.Y.
In vitro pharmacogenomic database and chemosensitivity predictive genes in gastric cancer.
Genomics 93:52-61(2009)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22531121; DOI=10.2739/kurumemedj.58.73
Koga A., Aoyagi K., Imaizumi T., Miyagi M., Shirouzu K.
Comparison between the gastric cancer cell line MKN-45 and the high-potential peritoneal dissemination gastric cancer cell line MKN-45P.
Kurume Med. J. 58:73-79(2011)

PubMed=22336246; DOI=10.1016/j.bmc.2012.01.017
Kong D., Yamori T.
JFCR39, a panel of 39 human cancer cell lines, and its application in the discovery and development of anticancer drugs.
Bioorg. Med. Chem. 20:1947-1951(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23671654; DOI=10.1371/journal.pone.0063056
Lu Y., Soong T.D., Elemento O.
A novel approach for characterizing microsatellite instability in cancer cells.
PLoS ONE 8:E63056-E63056(2013)

PubMed=24807215; DOI=10.1038/ncomms4830
Liu J., McCleland M., Stawiski E.W., Gnad F., Mayba O., Haverty P.M., Durinck S., Chen Y.-J., Klijn C., Jhunjhunwala S., Lawrence M., Liu H., Wan Y., Chopra V., Yaylaoglu M.B., Yuan W., Ha C., Gilbert H.N., Reeder J., Pau G., Stinson J., Stern H.M., Manning G., Wu T.D., Neve R.M., de Sauvage F.J., Modrusan Z., Seshagiri S., Firestein R., Zhang Z.
Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.
Nat. Commun. 5:3830-3830(2014)

PubMed=25960936; DOI=10.4161/21624011.2014.954893
Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:E954893-E954893(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections CCTCC; GDC0220
DSMZ; ACC-409
IBRC; C10137
JCRB; JCRB0254
KCLB; 80103
NCBI_Iran; C615
RCB; RCB1001
Cell line databases/resources CCLE; MKN45_STOMACH
CCRID; 3111C0001CCC000229
Cell_Model_Passport; SIDM00247
CGH-DB; 120-1
CGH-DB; 9022-4
Cosmic-CLP; 925340
DepMap; ACH-000356
LINCS_LDP; LCL-1535
Ontologies BTO; BTO:0001225
CLO; CLO_0007733
CLO; CLO_0050797
EFO; EFO_0002832
MCCL; MCC:0000329
Biological sample resources BioSample; SAMN03473237
BioSample; SAMN03473585
BioSample; SAMN10988555
Chemistry resources ChEMBL-Cells; CHEMBL3307603
ChEMBL-Targets; CHEMBL614354
GDSC; 925340
PharmacoDB; MKN45_938_2019
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM552364
GEO; GSM562395
GEO; GSM618013
GEO; GSM618014
GEO; GSM618015
GEO; GSM618016
GEO; GSM827532
GEO; GSM828836
GEO; GSM844607
GEO; GSM887324
GEO; GSM888400
GEO; GSM1374681
GEO; GSM1670113
Other Wikidata; Q28473066
Polymorphism and mutation databases Cosmic; 685596
Cosmic; 801339
Cosmic; 848147
Cosmic; 848373
Cosmic; 873711
Cosmic; 877042
Cosmic; 887256
Cosmic; 888827
Cosmic; 897535
Cosmic; 918497
Cosmic; 922696
Cosmic; 925340
Cosmic; 926114
Cosmic; 968349
Cosmic; 983727
Cosmic; 1001651
Cosmic; 1001655
Cosmic; 1007602
Cosmic; 1090461
Cosmic; 1187283
Cosmic; 1223589
Cosmic; 1329185
Cosmic; 1436026
Cosmic; 1460707
Cosmic; 1518241
Cosmic; 1518255
Cosmic; 1582430
Cosmic; 1627265
Cosmic; 1995510
Cosmic; 2036663
IARC_TP53; 21633
IARC_TP53; 30213
LiGeA; CCLE_725
Entry history
Entry creation04-Apr-2012
Last entry updated19-Dec-2019
Version number32