Cellosaurus MRC-5 (CVCL_0440)

Cell line name MRC-5
Synonyms MRC5; MRC 5; MRCV; MRC-V; Medical Research Council cell strain-5
Accession CVCL_0440
Resource Identification Initiative To cite this cell line use: MRC-5 (RRID:CVCL_0440)
Comments Group: Vaccine production cell line.
Biotechnology: Used for the production of the Human Diploid Cell Vaccine (HDCV) against rabies (Trade name: Imovax Rabies).
Biotechnology: Used for the production of the Hepatitis A vaccines Avaxim, Epaxal, Havrix and Vaqta.
Biotechnology: Used for the production of the live varicella virus vaccine (Trade name: Varivax).
Biotechnology: Used for the production of the live zoster vaccine (Trade name: Zostavax).
Characteristics: Senesces at 26 PDL (PubMed=17395773).
Doubling time: ~34 hours (lot 06022011), ~1.5 days (lot 05202005), ~2 days (lot 05192003), 36 hours (lot 041392), ~5 days (lot 031098), ~38 hours (lot 04212016) (JCRB).
Omics: DNA methylation analysis.
Omics: Proteome analysis by 2D-DE.
Omics: Proteome analysis by 2D-DE/MS.
Omics: Transcriptome analysis.
Misspelling: MCR-5; Occasionally.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_2837 (A9(Neo1))CVCL_2838 (A9(Neo11))CVCL_2844 (A9(Neo19))
CVCL_2846 (A9(Neo20))CVCL_2848 (A9(Neo4))CVCL_F664 (Flow13000)
CVCL_JG29 (HuS-L12/10)CVCL_4W54 (HuSI-L23)CVCL_RG58 (iPSC D1)
CVCL_RG59 (iPSC D4)CVCL_RB14 (IRR-MRC-5)CVCL_4W64 (L12.28-1/1)
CVCL_U651 (L12.9-2(1))CVCL_U661 (L23immo)CVCL_E260 (MRC-5 CV1)
CVCL_2621 (MRC-5 pd13)CVCL_2622 (MRC-5 pd19)CVCL_2623 (MRC-5 pd25)
CVCL_2624 (MRC-5 pd30)CVCL_JF54 (MRC-5 PDL12)CVCL_H749 (MRC-5 PDL27)
CVCL_H748 (MRC-5 PDL40)CVCL_8B13 (MRC-5 PDL5.51)CVCL_2625 (MRC-5 SV1 TG1)
CVCL_2626 (MRC-5 SV1 TG2)CVCL_3027 (MRC-5-30)CVCL_3028 (MRC-5-40)
CVCL_3029 (MRC-5-50)CVCL_D690 (MRC-5V1)CVCL_2627 (MRC-5V2)
CVCL_D923 (MRC-iPS-1)CVCL_D924 (MRC-iPS-10)CVCL_D925 (MRC-iPS-100)
CVCL_D926 (MRC-iPS-101)CVCL_8343 (MRC-iPS-11)CVCL_D927 (MRC-iPS-12)
CVCL_D928 (MRC-iPS-13)CVCL_D929 (MRC-iPS-14)CVCL_D930 (MRC-iPS-15)
CVCL_D931 (MRC-iPS-16)CVCL_D932 (MRC-iPS-17)CVCL_8367 (MRC-iPS-18)
CVCL_D933 (MRC-iPS-19)CVCL_D934 (MRC-iPS-2)CVCL_D935 (MRC-iPS-20)
CVCL_D936 (MRC-iPS-21)CVCL_8426 (MRC-iPS-22)CVCL_D937 (MRC-iPS-23)
CVCL_D938 (MRC-iPS-24)CVCL_8433 (MRC-iPS-25)CVCL_D939 (MRC-iPS-26)
CVCL_8434 (MRC-iPS-27)CVCL_D940 (MRC-iPS-28)CVCL_D941 (MRC-iPS-29)
CVCL_D942 (MRC-iPS-3)CVCL_D943 (MRC-iPS-30)CVCL_D944 (MRC-iPS-31)
CVCL_D945 (MRC-iPS-32)CVCL_D946 (MRC-iPS-33)CVCL_D947 (MRC-iPS-34)
CVCL_D948 (MRC-iPS-35)CVCL_D949 (MRC-iPS-36)CVCL_D950 (MRC-iPS-37)
CVCL_D951 (MRC-iPS-38)CVCL_D952 (MRC-iPS-39)CVCL_D953 (MRC-iPS-4)
CVCL_D954 (MRC-iPS-40)CVCL_D955 (MRC-iPS-41)CVCL_D956 (MRC-iPS-42)
CVCL_D957 (MRC-iPS-43)CVCL_D958 (MRC-iPS-44)CVCL_D959 (MRC-iPS-45)
CVCL_D960 (MRC-iPS-46)CVCL_D961 (MRC-iPS-47)CVCL_D962 (MRC-iPS-48)
CVCL_D963 (MRC-iPS-49)CVCL_D964 (MRC-iPS-5)CVCL_D965 (MRC-iPS-50)
CVCL_D966 (MRC-iPS-51)CVCL_D967 (MRC-iPS-52)CVCL_D968 (MRC-iPS-53)
CVCL_D969 (MRC-iPS-54)CVCL_D970 (MRC-iPS-55)CVCL_D971 (MRC-iPS-56)
CVCL_D972 (MRC-iPS-57)CVCL_D973 (MRC-iPS-58)CVCL_D974 (MRC-iPS-59)
CVCL_D975 (MRC-iPS-6)CVCL_D976 (MRC-iPS-60)CVCL_D977 (MRC-iPS-61)
CVCL_D978 (MRC-iPS-62)CVCL_D979 (MRC-iPS-63)CVCL_D980 (MRC-iPS-64)
CVCL_D981 (MRC-iPS-65)CVCL_D982 (MRC-iPS-66)CVCL_D983 (MRC-iPS-67)
CVCL_D984 (MRC-iPS-68)CVCL_D985 (MRC-iPS-69)CVCL_D986 (MRC-iPS-7)
CVCL_D987 (MRC-iPS-70)CVCL_D988 (MRC-iPS-71)CVCL_D989 (MRC-iPS-72)
CVCL_D990 (MRC-iPS-73)CVCL_D991 (MRC-iPS-74)CVCL_D992 (MRC-iPS-75)
CVCL_D993 (MRC-iPS-76)CVCL_D994 (MRC-iPS-77)CVCL_D995 (MRC-iPS-78)
CVCL_D996 (MRC-iPS-79)CVCL_D997 (MRC-iPS-8)CVCL_D998 (MRC-iPS-80)
CVCL_D999 (MRC-iPS-81)CVCL_E000 (MRC-iPS-82)CVCL_E001 (MRC-iPS-83)
CVCL_E002 (MRC-iPS-84)CVCL_E003 (MRC-iPS-85)CVCL_E004 (MRC-iPS-86)
CVCL_E005 (MRC-iPS-87)CVCL_E006 (MRC-iPS-88)CVCL_E007 (MRC-iPS-89)
CVCL_E008 (MRC-iPS-9)CVCL_E009 (MRC-iPS-90)CVCL_E010 (MRC-iPS-91)
CVCL_E011 (MRC-iPS-92)CVCL_E012 (MRC-iPS-93)CVCL_E013 (MRC-iPS-94)
CVCL_E014 (MRC-iPS-95)CVCL_E015 (MRC-iPS-96)CVCL_E016 (MRC-iPS-97)
CVCL_E017 (MRC-iPS-98)CVCL_E018 (MRC-iPS-99)CVCL_C975 (MRC5-iPS12)
CVCL_C974 (MRC5-iPS17)CVCL_V234 (MRC5-iPS2)CVCL_C976 (MRC5-RiPS-1.11)
CVCL_C977 (MRC5-RiPS-1.2)CVCL_C978 (MRC5-RiPS-1.3)CVCL_C979 (MRC5-RiPS-1.8)
CVCL_C980 (MRC5-RiPS-1.9)CVCL_Y625 (SR2)
Sex of cell Male
Age at sampling 14FW
Category Finite cell line
STR profile Source(s): AddexBio; ATCC; JCRB; KCLB; PubMed=11416159; PubMed=21637780; PubMed=25877200; RCB

Markers:
AmelogeninX,Y
CSF1PO11,12
D13S31711,14
D16S5399,11
D18S5115,21
D21S1131.2
D3S135815,17
D5S81811,12
D7S82010,11
D8S117913
FGA21,23
Penta D12
Penta E12,16
TH018
TPOX8
vWA15
Web pages https://en.wikipedia.org/wiki/MRC-5
https://strap.nci.nih.gov/celline_detail.php?sample_id=68
http://micro.magnet.fsu.edu/primer/techniques/fluorescence/gallery/cells/mrc5/mrc5cells.html
http://en.vircell.com/products/mrc-5-cell-line/
http://www.who.int/biologicals/BS%202077%20MRC-5.pdf
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-380.html
Publications

PubMed=4316953; DOI=10.1038/227168a0
Jacobs J.P., Jones C.M., Baille J.P.
Characteristics of a human diploid cell designated MRC-5.
Nature 227:168-170(1970)

PubMed=932048; DOI=10.1016/0092-1157(76)90018-4
Jacobs J.P.
The status of human diploid cell strain MRC-5 as an approved substrate for the production of viral vaccines.
J. Biol. Stand. 4:97-99(1976)

PubMed=1031681
Jacobs J.P.
Updated results on the karyology of the WI-38, MRC-5 and MRC-9 cell strains.
Dev. Biol. Stand. 37:155-156(1976)

PubMed=211143
Friedman H.M., Koropchak C.
Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of viruses from clinical specimens.
J. Clin. Microbiol. 7:368-371(1978)

PubMed=7065527; DOI=10.1164/arrd.1982.125.2.222
Hay R.J., Williams C.D., Macy M.L., Lavappa K.S.
Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.
Am. Rev. Respir. Dis. 125:222-232(1982)

PubMed=1965304; DOI=10.1002/elps.1150111203
Celis J.E., Dejgaard K., Madsen P., Leffers H., Gesser B., Honore B., Rasmussen H.H., Olsen E., Lauridsen J.B., Ratz G., Mouritzen S., Basse B., Hellerup M., Celis A., Puype M., Van Damme J., Vandekerckhove J.
The MRC-5 human embryonal lung fibroblast two-dimensional gel cellular protein database: quantitative identification of polypeptides whose relative abundance differs between quiescent, proliferating and SV40 transformed cells.
Electrophoresis 11:1072-1113(1990)

PubMed=10752125
Polianskaia G.G., Efremova T.N., Sakuta G.A.
The effect of mycoplasmal contamination of human embryonic lung cell line MRC-5 on the karyotypic variability.
Tsitologiia 42:190-195(2000)

PubMed=17395773; DOI=10.1634/stemcells.2006-0504
Sudo K., Kanno M., Miharada K., Ogawa S., Hiroyama T., Saijo K., Nakamura Y.
Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.
Stem Cells 25:1610-1617(2007)

PubMed=19657000
Rubporn A., Srisomsap C., Subhasitanont P., Chokchaichamnankit D., Chiablaem K., Svasti J., Sangvanich P.
Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line.
Cancer Genomics Proteomics 6:229-237(2009)

PubMed=21637780; DOI=10.1371/journal.pgen.1002085
Nishino K., Toyoda M., Yamazaki-Inoue M., Fukawatase Y., Chikazawa E., Sakaguchi H., Akutsu H., Umezawa A.
DNA methylation dynamics in human induced pluripotent stem cells over time.
PLoS Genet. 7:E1002085-E1002085(2011)

PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

CLPUB00391
Wadman M.
The vaccine race: science, politics, and the human costs of defeating disease.
(In) ISBN 9780525427537; pp.1-436; Viking; New York (2016)

Cross-references
Cell line collections AddexBio; T0016002/4922
ATCC; CCL-171
BCRC; 60023
BCRJ; 0180
CLS; 300395/NA
ECACC; 97112601 - Discontinued
ICLC; HL95001
IZSLER; BS CL 68
JCRB; IFO50073
JCRB; JCRB9008
KCB; KCB 89023YJ
KCB; KCB 200412YJ
KCLB; 10171
NCBI_Iran; C125
RCB; RCB0211
RCB; RCB0218
Cell line databases/resources CLDB; cl3563
CLDB; cl3564
CLDB; cl3566
CLDB; cl3567
CLDB; cl3568
CLDB; cl5168
CCRID; 3111C0001CCC000044
CCRID; 3111C0002000000002
CCRID; 3131C0001000200012
CCRID; 3142C0001000000032
CCRID; 3153C0001000000002
Ontologies BTO; BTO:0001590
CLO; CLO_0007865
CLO; CLO_0050055
EFO; EFO_0002835
MCCL; MCC:0000336
Biological sample resources BioSample; SAMN01821580
BioSample; SAMN01821702
BioSample; SAMN01821735
BioSample; SAMN03473183
Chemistry resources ChEMBL-Cells; CHEMBL3308499
ChEMBL-Targets; CHEMBL614181
Gene expression databases GEO; GSM248209
GEO; GSM248210
GEO; GSM520665
GEO; GSM520666
GEO; GSM520667
GEO; GSM579888
GEO; GSM579889
GEO; GSM607969
Other Wikidata; Q3273166
Proteomic databases PRIDE; PXD001909