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Cellosaurus MeWo (CVCL_0445)

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Cell line name MeWo
Synonyms MEWO; Mewo; Me Wo; Me-Wo; Mevo; SK-MEL-MeWo; Mel-MeWo; BI-Mel; EST50
Accession CVCL_0445
Resource Identification Initiative To cite this cell line use: MeWo (RRID:CVCL_0445)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: COSMIC cell lines project.
Part of: ERK genetic alteration cell panel (ATCC TCP-1033).
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Doubling time: ~32 hours (PBCF).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Array-based CGH.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: HLA class I peptidome analysis by proteomics.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations Homozygous for CDKN2A p.Arg80Ter (c.237_238CC>TT) (c.237_238delinsTT) (p.Pro94Leu; c.280_281CC>TT) (PubMed=17260012; PubMed=23851445; ATCC).
FGFR1 p.Pro252Ser (c.754C>T) (PubMed=23851445).
Heterozygous for MAPK3 p.Pro246Ser (c.736C>T) (ATCC).
TP53 p.Gln317Ter (c.949C>T) (PubMed=17260012; PubMed=23851445).
HLA typing Source: PubMed=15592718
Class I
HLA-AA*02:01,26:01
HLA-BB*14:02,38:01
HLA-CC*08:02,12:03
Class II
HLA-DPDPB1*04:01,04:02
HLA-DQDQB1*03:01:01,05:01
HLA-DRDRB1*11:01,01:02

Source: PubMed=25960936
Class I
HLA-AA*02:01,26:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African2.39
Native American1.31
East Asian, North0.6
East Asian, South0.77
South Asian7.24
European, North25.84
European, South61.86
Disease Cutaneous melanoma (NCIt: C3510)
Derived from metastatic site: Lymph node.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_AZ60 (MeWo CIS 0.01)CVCL_AZ74 (MeWo CIS 1)CVCL_AZ61 (MeWo ETO 0.1)
CVCL_AZ62 (MeWo ETO 1)CVCL_AZ75 (MeWo FOTE 4)CVCL_AZ76 (MeWo FOTE 40)
CVCL_Y663 (MeWo LNI 6I)CVCL_AZ63 (MeWo VIN 0.5)CVCL_AZ77 (MeWo VIN 5)
CVCL_U937 (MeWo-LC1)CVCL_J260 (Mewo-Luc)
Sex of cell Male
Age at sampling 78Y
Category Cancer cell line
STR profile Source(s): ATCC; CLS; Cosmic-CLP; ESTDAB; JCRB; PubMed=25877200

Markers:
AmelogeninX,Y
CSF1PO12 (ATCC; CLS; Cosmic-CLP; JCRB; PubMed=25877200)
10,12 (ESTDAB)
D3S135817
D5S81812,13
D7S82010,12
D8S117913,15
D13S3178 (Cosmic-CLP; ESTDAB)
8,9 (ATCC; CLS; JCRB; PubMed=25877200)
D16S53910,12
D18S5114,17
D21S1130,32.2
FGA22
Penta D10
Penta E5 (CLS)
5,15 (PubMed=25877200)
TH017,9
TPOX8,10 (ATCC; CLS; Cosmic-CLP; JCRB; PubMed=25877200)
9,11 (ESTDAB)
vWA15

Run an STR similarity search on this cell line
Web pages https://physics.cancer.gov/docs/bioresource/skin/NCI-PBCF-HTB65_MeWo_SOP-508.pdf
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-532.html
Publications

PubMed=978138; DOI=10.1084/jem.144.4.873
Shiku H., Takahashi T., Oettgen H.F., Old L.J.
Cell surface antigens of human malignant melanoma. II. Serological typing with immune adherence assays and definition of two new surface antigens.
J. Exp. Med. 144:873-881(1976)

PubMed=1067619; DOI=10.1073/pnas.73.9.3278
Carey T.E., Takahashi T., Resnick L.A., Oettgen H.F., Old L.J.
Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells.
Proc. Natl. Acad. Sci. U.S.A. 73:3278-3282(1976)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Fogh J., Fogh J.M., Orfeo T.
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Fogh J., Wright W.C., Loveless J.D.
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

DOI=10.1007/BF00199208
Bruggen J., Sorg C., Macher E.
Membrane associated antigens of human malignant melanoma V: Serological typing of cell lines using antisera from nonhuman primates.
Cancer Immunol. Immunother. 5:53-62(1978)

PubMed=6933476; DOI=10.1073/pnas.77.7.4260
Houghton A.N., Taormina M.C., Ikeda H., Watanabe T., Oettgen H.F., Old L.J.
Serological survey of normal humans for natural antibody to cell surface antigens of melanoma.
Proc. Natl. Acad. Sci. U.S.A. 77:4260-4264(1980)

DOI=10.1007/978-1-4615-7228-2_39
Houghton A.N., Oettgen H.F., Old L.J.
Malignant melanoma. Current status of the search for melanoma-specific antigens.
(In) Immunodermatology. Comprehensive Immunology, Vol 7; Safai B., Good R.A. (eds.); pp.557-576; Springer; Boston (1981)

DOI=10.1007/BF00205883
Bruggen J., Macher E., Sorg C.
Expression of surface antigens and its relation to parameters of malignancy in human malignant melanoma.
Cancer Immunol. Immunother. 10:121-127(1981)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
Wright W.C., Daniels W.P., Fogh J.
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=7017212; DOI=10.1093/jnci/66.6.1003
Pollack M.S., Heagney S.D., Livingston P.O., Fogh J.
HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.
J. Natl. Cancer Inst. 66:1003-1012(1981)

PubMed=7459858
Rousset M., Zweibaum A., Fogh J.
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=7175440; DOI=10.1084/jem.156.6.1755
Houghton A.N., Eisinger M., Albino A.P., Cairncross J.G., Old L.J.
Surface antigens of melanocytes and melanomas. Markers of melanocyte differentiation and melanoma subsets.
J. Exp. Med. 156:1755-1766(1982)

PubMed=6582512; DOI=10.1073/pnas.81.2.568
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=2068080; DOI=10.1073/pnas.88.14.6028
Cornil I., Theodorescu D., Man S., Herlyn M., Jambrosic J.A., Kerbel R.S.
Fibroblast cell interactions with human melanoma cells affect tumor cell growth as a function of tumor progression.
Proc. Natl. Acad. Sci. U.S.A. 88:6028-6032(1991)

PubMed=1383272; DOI=10.1172/JCI116007
Nip J., Shibata H., Loskutoff D.J., Cheresh D.A., Brodt P.
Human melanoma cells derived from lymphatic metastases use integrin alpha v beta 3 to adhere to lymph node vitronectin.
J. Clin. Invest. 90:1406-1413(1992)

PubMed=7908310; DOI=10.1080/09553009414550371
Muller W.-U., Bauch T., Streffer C., Niedereichholz F., Bocker W.
Comet assay studies of radiation-induced DNA damage and repair in various tumour cell lines.
Int. J. Radiat. Biol. 65:315-319(1994)

PubMed=9290701; DOI=10.1002/(SICI)1098-2744(199708)19:4<243::AID-MC5>3.0.CO;2-D
Jia L.-Q., Osada M., Ishioka C., Gamo M., Ikawa S., Suzuki T., Shimodaira H., Niitani T., Kudo T., Akiyama M., Kimura N., Matsuo M., Mizusawa H., Tanaka N., Koyama H., Namba M., Kanamaru R., Kuroki T.
Screening the p53 status of human cell lines using a yeast functional assay.
Mol. Carcinog. 19:243-253(1997)

PubMed=15592718; DOI=10.1007/s00262-004-0561-5
Rodriguez T., Mendez R., Roberts C.H., Ruiz-Cabello F., Dodi I.A., Lopez Nevot M.A., Paco L., Maleno I., Marsh S.G.E., Pawelec G., Garrido F.
High frequency of homozygosity of the HLA region in melanoma cell lines reveals a pattern compatible with extensive loss of heterozygosity.
Cancer Immunol. Immunother. 54:141-148(2005)

PubMed=17260012; DOI=10.1038/sj.onc.1210252
Jonsson G., Dahl C., Staaf J., Sandberg T., Bendahl P.-O., Ringner M., Guldberg P., Borg A.
Genomic profiling of malignant melanoma using tiling-resolution arrayCGH.
Oncogene 26:4738-4748(2007)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23851445; DOI=10.1158/1541-7786.MCR-13-0006
Dahl C., Christensen C., Jonsson G., Lorentzen A., Skjodt M.L., Borg A., Pawelec G., Guldberg P.
Mutual exclusivity analysis of genetic and epigenetic drivers in melanoma identifies a link between p14 ARF and RARbeta signaling.
Mol. Cancer Res. 11:1166-1178(2013)

PubMed=25960936; DOI=10.4161/21624011.2014.954893
Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.
A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.
OncoImmunology 3:E954893-E954893(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27600516; DOI=10.1007/s00262-016-1897-3
Gloger A., Ritz D., Fugmann T., Neri D.
Mass spectrometric analysis of the HLA class I peptidome of melanoma cell lines as a promising tool for the identification of putative tumor-associated HLA epitopes.
Cancer Immunol. Immunother. 65:1377-1393(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

Cross-references
Cell line collections ATCC; HTB-65
BCRC; 60540
BCRJ; 0358
CLS; 300285/p1765_MEWO
ECACC; 93082609
ICLC; HTL97019
JCRB; JCRB0066
Cell line databases/resources CLDB; cl3456
CLDB; cl3458
CCLE; MEWO_SKIN
Cell_Model_Passport; SIDM00545
Cosmic-CLP; 908128
DepMap; ACH-000987
ESTDAB; ESTDAB-050
LINCS_LDP; LCL-1253
Lonza; 437
Ontologies BTO; BTO:0003301
CLO; CLO_0007687
CLO; CLO_0007688
EFO; EFO_0006648
MCCL; MCC:0000341
Biological sample resources BioSample; SAMN01821578
BioSample; SAMN01821699
BioSample; SAMN03470821
BioSample; SAMN05292463
BioSample; SAMN10987991
Chemistry resources ChEMBL-Cells; CHEMBL3308024
ChEMBL-Targets; CHEMBL613300
GDSC; 908128
PharmacoDB; Mewo_918_2019
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM156016
GEO; GSM188224
GEO; GSM188254
GEO; GSM188313
GEO; GSM206525
GEO; GSM274688
GEO; GSM827470
GEO; GSM887310
GEO; GSM888386
GEO; GSM1670097
GEO; GSM3039511
GEO; GSM3039514
GEO; GSM3039515
Other Wikidata; Q54905336
Polymorphism and mutation databases Cosmic; 721835
Cosmic; 886829
Cosmic; 908128
Cosmic; 928689
Cosmic; 932713
Cosmic; 933141
Cosmic; 972284
Cosmic; 1047647
Cosmic; 1060422
Cosmic; 1132584
Cosmic; 1238113
Cosmic; 1303046
Cosmic; 1995506
Cosmic; 2036702
Cosmic; 2159442
Cosmic; 2163794
Cosmic; 2651875
IARC_TP53; 26074
LiGeA; CCLE_829
Entry history
Entry creation04-Apr-2012
Last entry update02-Jul-2020
Version number34