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Cellosaurus NCI-H295R (CVCL_0458)

[Text version]

Cell line name NCI-H295R
Synonyms NCI H295R; NCIH295R; H295R; H-295R; H295R-S1
Accession CVCL_0458
Resource Identification Initiative To cite this cell line use: NCI-H295R (RRID:CVCL_0458)
Comments Characteristics: Grows in a medium called Nu-Serum type I.
Doubling time: 25 hours (PubMed=29371329).
Omics: SNP array analysis.
Derived from sampling site: Adrenal gland.
Sequence variations Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45Pro (c.133T>C); ClinVar=VCV000017589; Zygosity=Heterozygous (from parent cell line).
Disease Adrenal cortex carcinoma (NCIt: C9325)
Adrenocortical carcinoma (ORDO: Orphanet_1501)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0456 (NCI-H295)
Children:
CVCL_5V09 (HAC13)CVCL_S898 (HAC15)CVCL_5V08 (HAC50)
Sex of cell Female
Age at sampling 48Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS

Markers:
AmelogeninX
CSF1PO10,12
D2S133825
D3S135815,16
D5S81812
D7S8209,12
D8S117913
D13S31713
D16S53911
D18S5117
D19S43313
D21S1132.2
FGA19.2,24
Penta D8
Penta E5,12
TH019.3
TPOX8
vWA17,18

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/H295R
Publications

PubMed=15541570; DOI=10.1016/j.mce.2003.12.020
Rainey W.E., Saner K., Schimmer B.P.
Adrenocortical cell lines.
Mol. Cell. Endocrinol. 228:23-38(2004)

PubMed=18000308; DOI=10.1677/JOE-07-0166
Samandari E., Kempna P., Nuoffer J.M., Hofer G., Mullis P.E., Fluck C.E.
Human adrenal corticocarcinoma NCI-H295R cells produce more androgens than NCI-H295A cells and differ in 3beta-hydroxysteroid dehydrogenase type 2 and 17,20 lyase activities.
J. Endocrinol. 195:459-472(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=21924324; DOI=10.1016/j.mce.2011.08.041
Wang T., Rainey W.E.
Human adrenocortical carcinoma cell lines.
Mol. Cell. Endocrinol. 351:58-65(2012)

PubMed=22266826; DOI=10.1055/s-0031-1298019
Wang T., Rowland J.G., Parmar J., Nesterova M., Seki T., Rainey W.E.
Comparison of aldosterone production among human adrenocortical cell lines.
Horm. Metab. Res. 44:245-250(2012)

PubMed=23951072; DOI=10.1371/journal.pone.0071022
Nishi H., Arai H., Momiyama T.
NCI-H295R, a human adrenal cortex-derived cell line, expresses purinergic receptors linked to Ca(2)(+)-mobilization/influx and cortisol secretion.
PLoS ONE 8:E71022-E71022(2013)

PubMed=29371329; DOI=10.1530/ERC-17-0447
Kiseljak-Vassiliades K., Zhang Y., Bagby S.M., Kar A., Pozdeyev N., Xu M., Gowan K., Sharma V., Raeburn C.D., Albuja-Cruz M.B., Jones K.L., Fishbein L., Schweppe R.E., Somerset H.L., Pitts T.M., Leong S., Wierman M.E.
Development of new preclinical models to advance adrenocortical carcinoma research.
Endocr. Relat. Cancer 25:437-451(2018)

Cross-references
Cell line collections ATCC; CRL-2128
CLS; 300483/p789_NCI-H295R
KCB; KCB 201509YJ
Cell line databases/resources CCRID; 3111C0001CCC000256
IGRhCellID; NCIH295R
Lonza; 378
Ontologies BTO; BTO:0002588
CLO; CLO_0008082
EFO; EFO_0002289
MCCL; MCC:0000355
Chemistry resources ChEMBL-Cells; CHEMBL3307998
ChEMBL-Targets; CHEMBL612593
Gene expression databases ArrayExpress; E-MTAB-38
Other Wikidata; Q25323725
Polymorphism and mutation databases Cosmic; 1172633
Cosmic; 1191070
Cosmic; 1622873
Cosmic; 2364101
Cosmic; 2574494
Progenetix; CVCL_0458
Entry history
Entry creation04-Apr-2012
Last entry update20-May-2021
Version number20