Cellosaurus NCI-H460 (CVCL_0459)

Cell line name NCI-H460
Synonyms NCI.H460; H460; H-460; NCIH460; NCI-HUT-460; NCI-460
Accession CVCL_0459
Resource Identification Initiative To cite this cell line use: NCI-H460 (RRID:CVCL_0459)
Comments Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: EGFR genetic alteration cell panel (ATCC TCP-1027).
Part of: JFCR39 cancer cell line panel.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
Part of: NCI60 cancer cell line panel.
Doubling time: 33 hours (in RPMI 1640+10% FBS), 60 hours (in ACL-3), 42 hours (in ACL-3+BSA) (PubMed=3940644); 17.8 hours (NCI-DTP); ~23 hours (DSMZ).
Microsatellite instability: Stable (MSS) (Sanger).
Sequence variation: Homozygous for KRAS p.Gln61His (PubMed=17088437).
Sequence variation: Heterozygous for PIK3CA p.Glu545Lys (PubMed=17088437).
Sequence variation: Homozygous for STK11 p.Gln37Ter (PubMed=17088437).
Omics: Array-based CGH.
Omics: CNV analysis.
Omics: Deep exome analysis.
Omics: Deep phosphoproteome analysis.
Omics: Deep proteome analysis.
Omics: Deep RNAseq analysis.
Omics: Fluorescence phenotype profiling.
Omics: lncRNA expression profiling.
Omics: Metabolome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Disease Large cell lung carcinoma (NCIt: C4450)
Derived from metastatic site: Pleural effusion.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children: CVCL_LB67 (NCI-H460 MX10); CVCL_J264 (NCI-H460-Luc); CVCL_5J23 (NCI-H460-luc2); CVCL_5J07 (NCI-H460-Red-Fluc); CVCL_3969 (NCI-H460M); CVCL_KZ69 (PathHunter NCI-H460 CDC25A Degradation); CVCL_8113 (SEG-1)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; Cosmic-CLP; DSMZ; KCLB; PubMed=19372543; PubMed=25877200

Markers:
AmelogeninX,Y
CSF1PO11,12
D13S31713
D16S5399
D18S5113,15
D19S43314
D21S1130
D2S133817,25
D3S135815,18
D5S8189,10
D7S8209,12
D8S117912
FGA21,23
Penta D11,13
Penta E5
TH019.3
TPOX8 (AddexBio; ATCC; Cosmic-CLP; DSMZ; PubMed=19372543; PubMed=25877200)
9.3 (KCLB)
vWA17
Web pages https://dtp.cancer.gov/discovery_development/nci-60/cell_list.htm
https://strap.nci.nih.gov/celline_detail.php?sample_id=12
http://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-613.html
http://tcpaportal.org/mclp/
Publications

PubMed=3940644
Brower M., Carney D.N., Oie H.K., Gazdar A.F., Minna J.D.
Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.
Cancer Res. 46:798-806(1986)

PubMed=2041050; DOI=10.1093/jnci/83.11.757
Monks A., Scudiero D.A., Skehan P., Shoemaker R.H., Paull K., Vistica D.T., Hose C., Langley J., Cronise P., Vaigro-Wolff A., Gray-Goodrich M., Campbell H., Mayo J., Boyd M.
Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.
J. Natl. Cancer Inst. 83:757-766(1991)

PubMed=1563005
Giaccone G., Battey J., Gazdar A.F., Oie H., Draoui M., Moody T.W.
Neuromedin B is present in lung cancer cell lines.
Cancer Res. 52:2732s-2736s(1992)

PubMed=8385084; DOI=10.1111/j.1349-7006.1993.tb02851.x
Suzuki S., Takahashi T., Nakamura S., Koike K., Ariyoshi Y., Takahashi T., Ueda R.
Alterations of integrin expression in human lung cancer.
Jpn. J. Cancer Res. 84:168-174(1993)

PubMed=8626706; DOI=10.1074/jbc.271.19.11477
Quinn K.A., Treston A.M., Unsworth E.J., Miller M.-J., Vos M., Grimley C., Battey J., Mulshine J.L., Cuttitta F.
Insulin-like growth factor expression in human cancer cell lines.
J. Biol. Chem. 271:11477-11483(1996)

PubMed=8806092; DOI=10.1002/jcb.240630505
Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. 63 Suppl. 24:32-91(1996)

PubMed=10700174; DOI=10.1038/73432
Ross D.T., Scherf U., Eisen M.B., Perou C.M., Rees C., Spellman P., Iyer V., Jeffrey S.S., Van de Rijn M., Waltham M., Pergamenschikov A., Lee J.C.F., Lashkari D., Shalon D., Myers T.G., Weinstein J.N., Botstein D., Brown P.O.
Systematic variation in gene expression patterns in human cancer cell lines.
Nat. Genet. 24:227-235(2000)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Modi S., Kubo A., Oie H., Coxon A.B., Rehmatulla A., Kaye F.J.
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=15746151; DOI=10.1093/hmg/ddi092
Izumi H., Inoue J., Yokoi S., Hosoda H., Shibata T., Sunamori M., Hirohashi S., Inazawa J., Imoto I.
Frequent silencing of DBC1 is by genetic or epigenetic mechanisms in non-small cell lung cancers.
Hum. Mol. Genet. 14:997-1007(2005)

PubMed=17088437; DOI=10.1158/1535-7163.MCT-06-0433
Ikediobi O.N., Davies H., Bignell G.R., Edkins S., Stevens C., O'Meara S., Santarius T., Avis T., Barthorpe S., Brackenbury L., Buck G., Butler A., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Hunter C., Jenkinson A., Jones D., Kosmidou V., Lugg R., Menzies A., Mironenko T., Parker A., Perry J., Raine K., Richardson D., Shepherd R., Small A., Smith R., Solomon H., Stephens P., Teague J.W., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Reinhold W.C., Weinstein J.N., Stratton M.R., Futreal P.A., Wooster R.
Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.
Mol. Cancer Ther. 5:2606-2612(2006)

PubMed=17332333; DOI=10.1158/0008-5472.CAN-06-3339
Okabe T., Okamoto I., Tamura K., Terashima M., Yoshida T., Satoh T., Takada M., Fukuoka M., Nakagawa K.
Differential constitutive activation of the epidermal growth factor receptor in non-small cell lung cancer cells bearing EGFR gene mutation and amplification.
Cancer Res. 67:2046-2053(2007)

PubMed=19372543; DOI=10.1158/1535-7163.MCT-08-0921
Lorenzi P.L., Reinhold W.C., Varma S., Hutchinson A.A., Pommier Y., Chanock S.J., Weinstein J.N.
DNA fingerprinting of the NCI-60 cell line panel.
Mol. Cancer Ther. 8:713-724(2009)

PubMed=19472407; DOI=10.1002/humu.21028
Blanco R., Iwakawa R., Tang M., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.
A gene-alteration profile of human lung cancer cell lines.
Hum. Mutat. 30:1199-1206(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=22336246; DOI=10.1016/j.bmc.2012.01.017
Kong D., Yamori T.
JFCR39, a panel of 39 human cancer cell lines, and its application in the discovery and development of anticancer drugs.
Bioorg. Med. Chem. 20:1947-1951(2012)

PubMed=22384151; DOI=10.1371/journal.pone.0032096
Lee J.-S., Kim Y.K., Kim H.J., Hajar S., Tan Y.L., Kang N.-Y., Ng S.H., Yoon C.N., Chang Y.-T.
Identification of cancer cell-line origins using fluorescence image-based phenomic screening.
PLoS ONE 7:E32096-E32096(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.K., Yu J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L., Winckler W., Reich M., Li N., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22628656; DOI=10.1126/science.1218595
Jain M., Nilsson R., Sharma S., Madhusudhan N., Kitami T., Souza A.L., Kafri R., Kirschner M.W., Clish C.B., Mootha V.K.
Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation.
Science 336:1040-1044(2012)

PubMed=23381221; DOI=10.3892/or.2013.2266
Suzuki M., Shiraishi K., Eguchi A., Ikeda K., Mori T., Yoshimoto K., Ohba Y., Yamada T., Ito T., Baba Y., Baba H.
Aberrant methylation of LINE-1, SLIT2, MAL and IGFBP7 in non-small cell lung cancer.
Oncol. Rep. 29:1308-1314(2013)

PubMed=23856246; DOI=10.1158/0008-5472.CAN-12-3342
Abaan O.D., Polley E.C., Davis S.R., Zhu Y.J., Bilke S., Walker R.L., Pineda M., Gindin Y., Jiang Y., Reinhold W.C., Holbeck S.L., Simon R.M., Doroshow J.H., Pommier Y., Meltzer P.S.
The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology.
Cancer Res. 73:4372-4382(2013)

PubMed=23933261; DOI=10.1016/j.celrep.2013.07.018
Moghaddas Gholami A., Hahne H., Wu Z., Auer F.J., Meng C., Wilhelm M., Kuster B.
Global proteome analysis of the NCI-60 cell line panel.
Cell Rep. 4:609-620(2013)

PubMed=24135919; DOI=10.1038/ncomms3617
Balbin O.A., Prensner J.R., Sahu A., Yocum A., Shankar S., Malik R., Fermin D., Dhanasekaran S.M., Chandler B., Thomas D., Beer D.G., Cao X., Nesvizhskii A.I., Chinnaiyan A.M.
Reconstructing targetable pathways in lung cancer by integrating diverse omics data.
Nat. Commun. 4:2617-2617(2013)

PubMed=24279929; DOI=10.1186/2049-3002-1-20
Dolfi S.C., Chan L.L.-Y., Qiu J., Tedeschi P.M., Bertino J.R., Hirshfield K.M., Oltvai Z.N., Vazquez A.
The metabolic demands of cancer cells are coupled to their size and protein synthesis rates.
Cancer Metab. 1:20-20(2013)

PubMed=24670534; DOI=10.1371/journal.pone.0092047
Varma S., Pommier Y., Sunshine M., Weinstein J.N., Reinhold W.C.
High resolution copy number variation data in the NCI-60 cancer cell lines from whole genome microarrays accessible through CellMiner.
PLoS ONE 9:E92047-E92047(2014)

PubMed=25120651; DOI=10.3892/ol.2014.2234
Suzuki M., Ikeda K., Shiraishi K., Eguchi A., Mori T., Yoshimoto K., Shibata H., Ito T., Baba Y., Baba H.
Aberrant methylation and silencing of IRF8 expression in non-small cell lung cancer.
Oncol. Lett. 8:1025-1030(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z., Liu H., Degenhardt J., Mayba O., Gnad F., Liu J., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=27377824; DOI=10.1038/sdata.2016.52
Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.
Sci. Data 3:160052-160052(2016)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X., Egan R.K., Liu Q., Mironenko T., Mitropoulos X., Richardson L., Wang J., Zhang T., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=27807467; DOI=10.1186/s13100-016-0078-4
Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.
A map of mobile DNA insertions in the NCI-60 human cancer cell panel.
Mob. DNA 7:20-20(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

Cross-references
Cell line collections AddexBio; C0016003/39
ATCC; HTB-177
BCRC; 60373
CCLV; CCLV-RIE 0962
DSMZ; ACC-737
IZSLER; BS TCL 197
KCB; KCB 2010102YJ
KCLB; 30177
NCI-DTP; NCI-H460
Cell line databases/resources CLDB; cl1548
CCLE; NCIH460_LUNG
CCRID; 3111C0001CCC000355
CCRID; 3131C0001000700039
CGH-DB; 199-1
CGH-DB; 9186-4
Cosmic-CLP; 905943
GDSC; 905943
IGRhCellID; NCI-H460%20GEO
LINCS_LDP; LCL-1784
SKY/M-FISH/CGH; 2798
Ontologies BTO; BTO:0002207
CLO; CLO_0003601
CLO; CLO_0008089
EFO; EFO_0003044
MCCL; MCC:0000356
Biological sample resources BioSample; SAMN03471184
ENCODE; ENCBS398KLJ
ENCODE; ENCBS446PPO
ENCODE; ENCBS460OUE
ENCODE; ENCBS514AAA
ENCODE; ENCBS515AAA
ENCODE; ENCBS641PGO
ENCODE; ENCBS686KKP
ENCODE; ENCBS798ZFS
ENCODE; ENCBS814QPR
Chemistry resources ChEMBL-Cells; CHEMBL3307677
ChEMBL-Targets; CHEMBL396
Gene expression databases GEO; GSM2152
GEO; GSM50216
GEO; GSM50279
GEO; GSM206493
GEO; GSM253335
GEO; GSM274737
GEO; GSM274738
GEO; GSM385522
GEO; GSM385533
GEO; GSM434334
GEO; GSM513936
GEO; GSM514322
GEO; GSM750809
GEO; GSM784193
GEO; GSM794288
GEO; GSM799343
GEO; GSM799406
GEO; GSM827469
GEO; GSM844652
GEO; GSM844651
GEO; GSM847076
GEO; GSM887430
GEO; GSM888510
GEO; GSM1153414
GEO; GSM1181352
GEO; GSM1181361
GEO; GSM1374746
GEO; GSM1374747
GEO; GSM1374748
GEO; GSM1557134
GEO; GSM2124676
Polymorphism and mutation databases Cosmic; 722064
Cosmic; 724876
Cosmic; 755469
Cosmic; 844595
Cosmic; 875852
Cosmic; 877263
Cosmic; 877407
Cosmic; 897508
Cosmic; 905943
Cosmic; 911996
Cosmic; 931371
Cosmic; 947356
Cosmic; 980964
Cosmic; 1006534
Cosmic; 1017826
Cosmic; 1028956
Cosmic; 1032436
Cosmic; 1044245
Cosmic; 1047102
Cosmic; 1089231
Cosmic; 1092621
Cosmic; 1146917
Cosmic; 1154605
Cosmic; 1152502
Cosmic; 1188592
Cosmic; 1219077
Cosmic; 1239922
Cosmic; 1305349
Cosmic; 1312334
Cosmic; 1434967
Cosmic; 1436013
Cosmic; 1477420
Cosmic; 1802310
Cosmic; 1870275
Cosmic; 1945869
Cosmic; 1995579
Cosmic; 1998463
Cosmic; 2042872
Cosmic; 2125199
Cosmic; 2433756
Cosmic; 2630413