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Cellosaurus NCI-H157 (CVCL_0463)

[Text version]

Cell line name NCI-H157
Synonyms NCl-H157; NCI H157; H157; H-157; NCI-157
Accession CVCL_0463
Resource Identification Initiative To cite this cell line use: NCI-H157 (RRID:CVCL_0463)
Comments Problematic cell line: Contaminated. NCI-H157 and NCI-H1264 have been shown to be identical.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00412.
Doubling time: 53 hours (in RPMI 1640 + 10% FBS), 90 hours (in ACL-3 + BSA) (PubMed=3940644).
Omics: Deep proteome analysis.
Omics: miRNA expression profiling.
Omics: Protein expression by reverse-phase protein arrays.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Sequence variations CDKN2A p.Glu69Ter (c.205G>T) (p.Gly83Val; c.248G>T) (PubMed=30038707).
KRAS p.Gly12Arg (c.34G>C) (PubMed=1311061; PubMed=12068308).
TP53 p.Glu298Ter (c.892G>T) (PubMed=1311061).
Disease Squamous cell lung carcinoma (NCIt: C3493)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_VD88 (H157R)
Originate from same individual CVCL_3970 ! NCI-H1264
Sex of cell Male
Age at sampling 59Y
Category Cancer cell line
STR profile Source(s): ATCC; CCRID

Markers:
AmelogeninX
CSF1PO12
D2S133821,22
D3S135817,18
D5S81810,13
D7S82012
D8S117914,16
D13S31712
D16S53912,13
D18S5113,15
D19S43311,13
D21S1132
FGA22,23
TH017,9
TPOX6,12
vWA15

Run an STR similarity search on this cell line
Web pages http://www.cellresource.cn/fdetail.aspx?id=113
https://www.atcc.org/support/faqs/05d55/Cross-contamination%20of%20ATCC%20CRL-5802%20and%20ATCC%20CRL-5860-931.aspx
https://www.atcc.org/support/faqs/78ebc/Contaminating%20Line%20for%20ATCC%20CRL5802%20vs%20ATCC%20CRL5860-932.aspx
https://www.atcc.org/support/faqs/bb641/Misidentified%20Cell%20Lines%20ATCC%20CRL-5802%20and%20ATCC%20CRL-5860-930.aspx
http://iclac.org/wp-content/uploads/Cross-Contaminations_v9_distribution.xlsx
Publications

PubMed=6148444; DOI=10.1093/jnci/73.4.801
Morstyn G., Russo A., Carney D.N., Karawya E., Wilson S.H., Mitchell J.B.
Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.
J. Natl. Cancer Inst. 73:801-807(1984)

PubMed=3940644
Brower M., Carney D.N., Oie H.K., Gazdar A.F., Minna J.D.
Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.
Cancer Res. 46:798-806(1986)

PubMed=1311061
Mitsudomi T., Steinberg S.M., Nau M.M., Carbone D., D'Amico D., Bodner S., Oie H.K., Linnoila R.I., Mulshine J.L., Minna J.D., Gazdar A.F.
p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features.
Oncogene 7:171-180(1992)

PubMed=1563005
Giaccone G., Battey J., Gazdar A.F., Oie H.K., Draoui M., Moody T.W.
Neuromedin B is present in lung cancer cell lines.
Cancer Res. 52:2732s-2736s(1992)

PubMed=8626706; DOI=10.1074/jbc.271.19.11477
Quinn K.A., Treston A.M., Unsworth E.J., Miller M.-J., Vos M., Grimley C., Battey J., Mulshine J.L., Cuttitta F.
Insulin-like growth factor expression in human cancer cell lines.
J. Biol. Chem. 271:11477-11483(1996)

PubMed=8806092; DOI=10.1002/jcb.240630505
Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. 63 Suppl. 24:32-91(1996)

PubMed=9649128; DOI=10.1038/bjc.1998.361
Yi S., Chen J.-R., Viallet J., Schwall R.H., Nakamura T., Tsao M.-S.
Paracrine effects of hepatocyte growth factor/scatter factor on non-small-cell lung carcinoma cell lines.
Br. J. Cancer 77:2162-2170(1998)

PubMed=11005564; DOI=10.1038/sj.neo.7900094
Kohno T., Sato T., Takakura S., Takei K., Inoue K., Nishioka M., Yokota J.
Mutation and expression of the DCC gene in human lung cancer.
Neoplasia 2:300-305(2000)

PubMed=11369051; DOI=10.1016/S0165-4608(00)00363-0
Luk C., Tsao M.-S., Bayani J., Shepherd F., Squire J.A.
Molecular cytogenetic analysis of non-small cell lung carcinoma by spectral karyotyping and comparative genomic hybridization.
Cancer Genet. Cytogenet. 125:87-99(2001)

PubMed=12068308; DOI=10.1038/nature00766
Davies H., Bignell G.R., Cox C., Stephens P., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=19472407; DOI=10.1002/humu.21028
Blanco R., Iwakawa R., Tang M., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.
A gene-alteration profile of human lung cancer cell lines.
Hum. Mutat. 30:1199-1206(2009)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20624269; DOI=10.1186/1756-9966-29-75
Du L., Schageman J.J., Irnov I., Girard L., Hammond S.M., Minna J.D., Gazdar A.F., Pertsemlidis A.
MicroRNA expression distinguishes SCLC from NSCLC lung tumor cells and suggests a possible pathological relationship between SCLCs and NSCLCs.
J. Exp. Clin. Cancer Res. 29:75-75(2010)

PubMed=22961666; DOI=10.1158/2159-8290.CD-12-0112
Byers L.A., Wang J., Nilsson M.B., Fujimoto J., Saintigny P., Yordy J., Giri U., Peyton M., Fan Y.H., Diao L., Masrorpour F., Shen L., Liu W., Duchemann B., Tumula P., Bhardwaj V., Welsh J., Weber S., Glisson B.S., Kalhor N., Wistuba I.I., Girard L., Lippman S.M., Mills G.B., Coombes K.R., Weinstein J.N., Minna J.D., Heymach J.V.
Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.
Cancer Discov. 2:798-811(2012)

PubMed=23381221; DOI=10.3892/or.2013.2266
Suzuki M., Shiraishi K., Eguchi A., Ikeda K., Mori T., Yoshimoto K., Ohba Y., Yamada T., Ito T., Baba Y., Baba H.
Aberrant methylation of LINE-1, SLIT2, MAL and IGFBP7 in non-small cell lung cancer.
Oncol. Rep. 29:1308-1314(2013)

PubMed=25120651; DOI=10.3892/ol.2014.2234
Suzuki M., Ikeda K., Shiraishi K., Eguchi A., Mori T., Yoshimoto K., Shibata H., Ito T., Baba Y., Baba H.
Aberrant methylation and silencing of IRF8 expression in non-small cell lung cancer.
Oncol. Lett. 8:1025-1030(2014)

PubMed=26361996; DOI=10.1016/j.jprot.2015.09.003
Grundner-Culemann K., Dybowski J.N., Klammer M., Tebbe A., Schaab C., Daub H.
Comparative proteome analysis across non-small cell lung cancer cell lines.
J. Proteomics 130:1-10(2016)

PubMed=30038707; DOI=10.18632/oncotarget.25642
Du L., Zhao Z., Suraokar M., Shelton S.S., Ma X., Hsiao T.-H., Minna J.D., Wistuba I., Pertsemlidis A.
LMO1 functions as an oncogene by regulating TTK expression and correlates with neuroendocrine differentiation of lung cancer.
Oncotarget 9:29601-29618(2018)

Cross-references
Cell line collections ATCC; CRL-5802 - Discontinued
KCB; KCB 200814YJ
KCLB; 90157 - Discontinued
Cell line databases/resources CCRID; 3111C0001CCC000113
IGRhCellID; H157GEO
LINCS_LDP; LCL-1596
Ontologies BTO; BTO:0002283
EFO; EFO_0003118
MCCL; MCC:0000360
Biological sample resources BioSample; SAMN03151855
BioSample; SAMN03471738
Chemistry resources ChEMBL-Cells; CHEMBL3307706
ChEMBL-Targets; CHEMBL614783
Gene expression databases ArrayExpress; E-MTAB-783
GEO; GSM6296
GEO; GSM253365
GEO; GSM434267
GEO; GSM784238
GEO; GSM794274
Other Wikidata; Q54907822
Polymorphism and mutation databases Cosmic; 722053
Cosmic; 724867
Cosmic; 801588
Cosmic; 844816
Cosmic; 852006
Cosmic; 876171
Cosmic; 877258
Cosmic; 903569
Cosmic; 911847
Cosmic; 917987
Cosmic; 929971
Cosmic; 930486
Cosmic; 932921
Cosmic; 934546
Cosmic; 953987
Cosmic; 949208
Cosmic; 980960
Cosmic; 1028954
Cosmic; 1032432
Cosmic; 1146881
Cosmic; 1219061
Cosmic; 1239910
Cosmic; 1320185
Cosmic; 2560227
Cosmic; 2648437
Cosmic; 2668267
Cosmic; 2668329
IARC_TP53; 495
IARC_TP53; 28242
Proteomic databases PRIDE; PXD002556
Entry history
Entry creation04-Apr-2012
Last entry updated12-Mar-20
Version number28