Cellosaurus NPA (CVCL_0467)

Cell line name NPA
Synonyms NPA-87; NPA'87; NPA 87; NPA87; NPA87-1; UCLA NPA-87-1; UCLA NPA871
Accession CVCL_0467
Secondary accession CVCL_6475
Resource Identification Initiative To cite this cell line use: NPA (RRID:CVCL_0467)
Comments Problematic cell line: Contaminated. Shown to be a M14 derivative (PubMed=18713817, PubMed=20143388, PubMed=21868764). Originally thought to originate from a thyroid gland papillary carcinoma.
Sequence variation: Heterozygous for BRAF p.Val600Glu (from parent cell line).
Sequence variation: Heterozygous for TP53 p.Gly266Glu (from parent cell line).
Disease Amelanotic melanoma (NCIt: C3802)
Derived from metastatic site: Subcutaneous; right buttock.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1395 (M14)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): PubMed=18713817; PubMed=21868764

Markers:
AmelogeninX
CSF1PO11
D13S31712
D16S5399,13
D18S5113,17
D21S1130
D3S135814,16
D5S81811,12
D7S8208
D8S117913
FGA21
TH016,7
TPOX8,11
vWA16,18
Web pages http://iclac.org/wp-content/uploads/Cross-Contaminations-v8_0.pdf
Publications

PubMed=8871538; DOI=10.1007/BF00128960
Boghaert E.R., Ain K., Taylor K., Greenberg V.L., Fowler C., Zimmer S.G.
Quantitative and qualitative differences in growth, invasion and lung colonization of an anaplastic and a papillary human thyroid cancer cell line in vitro and in vivo.
Clin. Exp. Metastasis 14:440-450(1996)

PubMed=18713817; DOI=10.1210/jc.2008-1102
Schweppe R.E., Klopper J.P., Korch C., Pugazhenthi U., Benezra M., Knauf J.A., Fagin J.A., Marlow L.A., Copland J.A., Smallridge R.C., Haugen B.R.
Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification.
J. Clin. Endocrinol. Metab. 93:4331-4341(2008)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

Cross-references
Cell line databases/resources TOKU-E; 4068
Ontologies BTO; BTO:0004771
MCCL; MCC:0000365
Biological sample resources BioSample; SAMN03151835
Polymorphism and mutation databases Cosmic; 685605
Cosmic; 686727
Cosmic; 849968
Cosmic; 886547
Cosmic; 918183
Cosmic; 928835
Cosmic; 931239
Cosmic; 990533
Cosmic; 1005456
Cosmic; 1021384
Cosmic; 1071908
Cosmic; 1094772
Cosmic; 1132596
Cosmic; 1152122
Cosmic; 1155264
Cosmic; 1155328
Cosmic; 1161613
Cosmic; 1162848
Cosmic; 1176608
Cosmic; 1699416