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Cellosaurus P39 (CVCL_0478)

Cell line name P39
Synonyms P39/TSU; P39-TSU; P39 TSU; P39TSU; P39/Tsugane; P39/TSUGANE; TSUGANE; Tsugane
Accession CVCL_0478
Resource Identification Initiative To cite this cell line use: P39 (RRID:CVCL_0478)
Comments Problematic cell line: Contaminated. Shown to be a HL-60 derivative (PubMed=12592342; PubMed=20143388; PubMed=20421273). Originally thought to originate from the peripheral blood of a 69 year old male with acute myeloid leukemia.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00255.
Derived from sampling site: Peripheral blood.
Sequence variations Homozygous for CDKN2A p.Arg80Ter (c.238C>T) (from parent cell line).
Heterozygous for NRAS p.Gln61Leu (c.182A>T) (PubMed=9379676).
Disease Adult acute myeloid leukemia (NCIt: C9154)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0002 (HL-60)
Sex of cell Female
Age at sampling 35Y
Category Cancer cell line
STR profile Source(s): JCRB

Markers:
AmelogeninX
CSF1PO13,14
D13S3178,11
D16S53911
D5S81811,12
D7S82011,12
TH017,8
TPOX8,11
vWA15,16
Web pages http://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
http://iclac.org/wp-content/uploads/Cross-Contaminations_v9_distribution.xlsx
Publications

DOI=10.1007/978-1-4757-1647-4_13
Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=6597119; DOI=10.20772/cancersci1959.75.12_1100
Nagai M., Seki S., Kitahara T., Abe T., Minato K., Watanabe S., Shimoyama M.
A novel human myelomonocytoid cell line, P39/Tsugane, derived from overt leukemia following myelodysplastic syndrome.
Gann 75:1100-1107(1984)

PubMed=9379676; DOI=10.1016/S0145-2126(97)00036-2
Sheng X.-M., Kawamura M., Ohnishi H., Ida K., Hanada R., Kojima S., Kobayashi M., Bessho F., Yanagisawa M., Hayashi Y.
Mutations of the RAS genes in childhood acute myeloid leukemia, myelodysplastic syndrome and juvenile chronic myelocytic leukemia.
Leuk. Res. 21:697-701(1997)

PubMed=12592342; DOI=10.1038/sj.leu.2402799
Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.
False leukemia-lymphoma cell lines: an update on over 500 cell lines.
Leukemia 17:416-426(2003)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20421273; DOI=10.3324/haematol.2010.022988
Steensma D.P.
P39/Tsugane cells are a false cell line contaminated with HL-60 cells and are not suitable for mechanistic studies in myelodysplastic syndromes.
Haematologica 95:1229-1230(2010)

PubMed=23955599; DOI=10.1038/ng.2731
Kon A., Shih L.-Y., Minamino M., Sanada M., Shiraishi Y., Nagata Y., Yoshida K., Okuno Y., Bando M., Nakato R., Ishikawa S., Sato-Otsubo A., Nagae G., Nishimoto A., Haferlach C., Nowak D., Sato Y., Alpermann T., Nagasaki M., Shimamura T., Tanaka H., Chiba K., Yamamoto R., Yamaguchi T., Otsu M., Obara N., Sakata-Yanagimoto M., Nakamaki T., Ishiyama K., Nolte F., Hofmann W.K., Miyawaki S., Chiba S., Mori H., Nakauchi H., Koeffler H.P., Aburatani H., Haferlach T., Shirahige K., Miyano S., Ogawa S.
Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.
Nat. Genet. 45:1232-1237(2013)

Cross-references
Cell line collections JCRB; JCRB0092
Ontologies MCCL; MCC:0000376
Biological sample resources BioSample; SAMN03151775
BioSample; SAMN03471068
Other Wikidata; Q54937278
Polymorphism and mutation databases Cosmic; 717681
Cosmic; 947362
Cosmic; 998740
Cosmic; 2089663